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PELATIHAN PEMBUATAN SABUN PADAT SPIRULINA UNTUK SISWA SMA/SMK DI SURABAYA DAN SIDOARJO Diah Karunia Binawati; Sukarjati; susie Amilah
Jurnal Penamas Adi Buana Vol 4 No 2 (2021): Jurnal Penamas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/penamas.vol4.no2.a2701

Abstract

Abstrak Spirulina adalah sianobakterium multiseluler, walaupun mirip dengan bakteri tetapi Spirulina mempunyai mekanisme fotosintesis yang mirip dengan yang ditemukan pada tanaman. Spirulina merupakan mikroalga sel tunggal yang hidup di air dan berkembang biak dengan cara membelah diri ini dikenal sebagai sumber nutrisi nabati terlengkap yang kaya akan asam amino esensial, vitamin, mineral dan karotenoid (Sitorus, 2015). Kandungan nutrisi Spirulina yang lengkap mengakibatkan Spirulina semakin banyak digunakan untuk kebutuhan manusia baik dalam bidang pangan maupun kecantikan. Pelatihan pembuatan sabun padat transparan Spirulina bertujuan untuk memberikan pengetahuan dan ketrampilan kepada siswa SMA/SMK di Surabaya dan Sidoarjo tentang pengaplikasian ilmu dan teknologi dalam menghasilkan sabun padat transparan untuk membersihkan dan menyehatkan kulit wajah. Bahan yang digunakan adalah Chip soap 450 g, Lexaine C 0,06 g, Carbowax liquid 0,03 g, Propilen glikol 0,03 g, Fixolite 12 g, Spirulina 9 g, Alkohol 96 % 30 ml, parfum. Hasil pelatihan ini memberikan informasi tentang penggunaan Spirulina untuk pembuatan sabun padat transparan dan mempraktekkan secara langsung pembuatan sabun padat transparan, sehingga diharapkan dapat menumbuhkan jiwa entrepreneur siswa. Kata kunci: sabun padat transparan, kulit wajah, Spirulina Abstract Spirulina is a multicellular cyanobacterium, although similar to bacteria, but Spirulina has a photosynthesis mechanism that is similar to those of plants. Spirulina is a single cell microalga that lives in the water and reproduce by dividing itself. Spirulina is known as the most complete source of vegetable nutrition because it’s rich of essential amino acid, vitamin, mineral, and carotenoids (Sitorus, 2015). The complete nutrition in Spirulina makes it more widely known and used to fulfill the human needs, both in the fields of food and beauty. The training of making Spirulina transparent solid soap aims to provide knowledge and skills to students of high school and vocational school in Surabaya and Sidoarjo regarding the application of science and technology in producing transparent solid soap to cleanse and nourish the facial skin. The ingredients used are Chip soap 450 g, Lexaine C 0,06 g, Carbowax liquid 0,03 g, Propilen glikol 0,03 g, Fixolite 12 g, Spirulina 9 g, Alcohol 96% 30 ml, and Perfume. The purpose of this study is to give information and direct practice about the use of Spirulina to make transparent solid soap, so this training is expected to grow the entrepreneurial sense of the students. Keywords: transparent solid soap, facial skin, Spirulina
PENGARUH EKSTRAK KULIT DELIMA (Punica granatum) DAN RUMPUT TEKI (Cyperus rotundus) TERHADAP MORTALITAS LARVA NYAMUK Aedes aegypti Diah Karunia Binawati; Susie Amilah
WAHANA Vol 63 No 2 (2014)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.66 KB) | DOI: 10.36456/wahana.v63i2.1126

Abstract

Dengue Haemorrhagic Fever (DHF) is nowadays one of the most common public health problems in Indonesia, which lately the number of patients and the range of dissemination tend to be increasing. The most simple and effective way to attempt termination of the life cycle of the mosquito Aedes aegypti is to kill mosquito larvae. Frankly, the act of using arviciding or chemical pesticides often cause these following problems such as environmental pollution, the emergence of resistance and disruption of the ecological balance. The use of plants as a natural pesticide that is environmental -friendly is one of the alternatives that could be done by the society. One of the ways is by using pomegranate peel extract (Punica granatum) and nut-grass extract (Cyperus rotundus). The skin of pomegranate contains saponins, flavonoids and polyphenols, flavonoids and polyphenols. While the nut-grass (Cyperus rotundus) contain alkaloids, flavonoids, cineol, pinene, siperon, rotunal, siperenon, and siperol. This study aims to determine whether there is the effect of giving pomegranate peel extract (Punica granatum) and extract of the nut-grass (Cyperus rotundus) against larvae of Aedes Agypti mortality during a 24-hour treatment. The research design used was a complete randomized design, with pomegranate peel extract treatment (0%, 5%, 10%, 15% and 20%), and extract the nut-grass (0%, 5%, 15% and 20%), each treatment was given 3 repetitions, and observed mortality of larvae of Aedes Agypti after treated for 24 hours. The results showed that 1. There is an effect of pomegranate peel extract against Aedes aegypti larvae mortality for 24 hours, and at a level of 20% has the highest mortality rate (12.5 ± 2.43) 2. There is an effect of nut-grass extract extract against Aedes aegypti larvae mortality for 24 hours, and at a level of 20% has the highest mortality rate (6 ± 1.14) 3. The extract of pomegranate skin has the ability to kill the larvae of Aedes aegypti greater than the extract of nut-grass
PENGGUNAAN BERBAGAI MEDIA TANAM TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TANAMAN BROKOLI (Brassica oleracea varitalica) DAN BABY KAILAN (Brassica oleracea var. Alboglabra baley) Susie Amilah Amilah
WAHANA Vol 59 No 2 (2012)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.563 KB) | DOI: 10.36456/wahana.v59i2.1216

Abstract

Brokoli (Brassica oleracea varitalica L. Kelompok Italica) dan baby kailan (Brassica oleracea var. Alboglabra Imlay) adalah tanaman sayuran yang termasuk dalam suku kubis-kubisan atau Brassicaceae. Dalam brokoli terdapat berbagai macam kandungan, diantaranya adalah tinggi kalium, serat , folat, Vitamin C, kalsium , Vitamin K, karoten, lutein dan rendah sodium. Tujuan penelitian ini ialah untuk mengetahui pengaruh berbagai media tanam terhadap pertumbuhan tanaman brokoli dan untuk mengetahui media tanam yang memberikan pengaruh paling balk terhadap pertumbuhan tanaman brokoli. Perlakuan meliputi 4 macam media tanam, yaitu campuran tanah dan sekam, tanah, pupuk kompos dan sekam, tanah, kotoran kambing dan sekam dan tanah, kotoran sapi dan sekam. Ulangan yang digunakan ialah 6X, dan sampel yang digunakan ialah 24 tanaman brokoli. Analisis data yang digunakan ialah uji F (anava) satu arch atau satu faktor dengan percobaan rancangan acak lengkap (RAL). Pada basil alchir analisis data pertumbuhan diperoleh Fhitung 12,5 > Ftabel 3,10 dan data berat diperoleh Fhitung 9,29>Ftabel 3,10 dengan taraf significant 5%, dengan demikian Ha ditolak dan Hl diterima, berarti ada pengaruh nyata terhadap pemberian perlakuan. Dari uji BNT, didapat basil bahwa penggunaan media tanam yang berupa tanah, pupuk kompos dan sekam memberikan pengaruh yang terbaik terhadap pertumbuhan dan berat tanaman Brokoli (Brasica oleracea varitalica) baby kailan (Brassica oleracea var. Alboglabra baley) karena pupuk kompos mengandung mikrobia dalam jumlah cukup yang berperanan dalam proses dekomposisi bahan organik sehingga balk digunakan sebagai media tanam.
EFFECT OF CHERRY LEAF (Muntinga calabora) BIOINSECTICIDES EXTRACT TOWARDS MORTALITY OF WORM SOIL (Agrotis ipsilon) AND ARMYWORM (Spodoptera exiqua) ON PLANT LEEK (Allium fistolum) Diah Karunia Binawati; Susie Amilah
WAHANA Vol 61 No 2 (2013)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.034 KB) | DOI: 10.36456/wahana.v61i2.1267

Abstract

Leek plant (Allium fistulosum) that grown at Village Treceh Sajen Mojokerto District Pacetoften suffer from some loss that caused by the attacks from pests such as worm soil (Agrotisipsilon) and armyworm (Spodoptera exiqua). The use of synthetic insecticides is very dangerous,because the residue will affect the health of consumers. Cherry plant (Muntinga calabura) containsactive substances such as tannins, saponins and flavonoids that can be used as bioinsecticides.This study aims to determine whether there is an effect of cherry leaf bioinsecticidesextract towards mortality of worm soil and armyworm in a in vitro manner, and to observe thegrowth of leek plant at harvest scallion age 2 months. The study design used is a CompleteRandomized Design, with 4 treatments using cherry leaf extract (0%, 2.5%, 5% and 7.5%), andgiven 6 replications. Samples of worm soil and armyworm caterpillars each are 120 which itsmortality will be observed for 24 hours. Leek plant sample are 60 plants. The results of dataanalysis showed a 24-hour LC50 value for worm soil is 4.240% and the 24-hour LC50 values forarmyworm is 3,434%. These results indicate that the cherry leaf extract is more effective toannihilate armyworm than it is to annihilate worm soil.To observe the effect of cherry leaf bioinsecticides treatment extract towards the growth ofleek plant leaves were harvested 2 months of age. Results of test F (0.024) indicates that there is asignificant effect of cherry leaf extract towards the growth of leek plants. The results of the LSDanalysis of these data showed that the P3 treatment with a concentration of 7.5% is the one thatproduces the best growth of the plant.
DAYA HAMBAT EKSTRAK SAMBILOTO (ANDROGRAPHIS PANICULATA) DENGAN BERBAGAI KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI SALMONELLA TYPHI Susie Amilah; Agus Prasetyo
WAHANA Vol 56 No 1 (2011)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (909.199 KB) | DOI: 10.36456/wahana.v56i1.1391

Abstract

Andrographis paniculata atau yang dikenal dengan nama sambiloto adalah tumbuhan yang biasa dipakai oleh masyarakat untuk mengobati typoid. Tujuan penelitian ini adalah untuk mengetahui daya hambat ekstrak sambiloto terhadap pertumbuhan Salmonella typhi dan mengetahui konsentrasi yang paling efektif dalam menghambat pertumbuhan bakteri Salmonella typhi. Bahan uji yang digunakan adalah biakan bakteri Salmonella typhi yang diisolasi dari penderita yang dirawat di RSU Dr. H. Koesnadi Bondowoso, yang kemudian diuji dengan menggunakan ekstrak sambiloto dengan berbagai konsentrasi. Adapun konsentrasi yang digunakan adalah konsentrasi 5%, 10%, 20%, 30%, dan 40%. Pengamatan jumlah koloni kuman dilakukan dengan metode Total Plate Count (TPC) . Data dianalisis menggunakan uji F yang dilanjutkan dengan LSD. Kesimpulan penelitian ini menunjukkan bahwa Andrographis paniculata atau sambiloto dengan berbagai konsentrasi memiliki kemampuan dalam menghambat pertumbuhan bakteri salmonella typhi. Konsentrasi yang memberikan daya hambat terbaik adalah konsentrasi ekstrak 40%. Dengan demikian masyarakat dapat menggunakan sambiloto sebagai obat alternatif dalam melakukan pengobatan terhadap penderita typoid.
Potensi Ekstrak Rimpang Pacing (Costus speciosus), Daun Srikaya (Annona squamosa L.) dan Kombinasinya Terhadap Jumlah Folikel Primer dan Sekunder Pada Mencit Betina (Mus musculus) Purity Sabila Ajiningrum; Susie Amilah; Prafikka Galuh Widyaningtyas
SNHRP Vol. 2 (2019): Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Ke 2 Tahun 2019
Publisher : LPPM Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.168 KB)

Abstract

Pengembangan kontrasepsi dari bahan alami, terutama dari tanaman dapat dijadikan solusi sebagai bahan pengatur fertilitas. Berbagai senyawa bioaktif yang ada pada tumbuhan memiliki khasiat sebagai bahan pengatur fertilitas. Pacing (Costus speciosus) dan Srikaya (Annona squamosa L.) adalah salah satu dari sekian banyak tanaman yang dapat dimanfaaatkan sebagai bahan kontrasepsi. Penelitian ini bertujuan untuk membuktikan pengaruh pemberian ekstrak Rimpang pacing, daun Srikaya dan ekstrak kombinasinya terhadap jumlah folikel primer dan folikel sekunder pada mencit (Mus musculus). Jumlah folikel dihitung berdasarkan perkembangannya pada ovarium. Tiap mencit dihitung jumlah folikelnya pada tiga preparat dan pengamatan dilakukan di bawah mikroskop dengan perbesaran 40 kali. Penelitian ini menggunakan data yang dihitung menggunakan perangkat lunak SPSS. Metode analisis uji F (anova) satu arah dengan percobaan rancangan acak lengkap (RAL). Berdasarkan hasil penelitian menunjukkan adanya pengaruh pemberian ekstrak rimpang pacing, daun srikaya dan ekstrak kombinasinya terhadap jumlah folikel primer dan sekunder pada mencit. Penurunan jumlah folikel primer dan sekunder terbaik adalah pada pemberian ekstrak Rimpang Pacing dosis 250 mg/kg BB. Semakin tinggi dosis ekstrak yang diberikan maka semakin sedikit jumlah folikel yang terbentuk. Kata kunci : daun srikaya, jumlah folikel, rimpang pacing
PKM BUDIDAYA IKAN LELE (Clarias gariepinus) MENGGUNAKAN SISTEM BIOFLOK PADA GURU SMA SE-JAWA TIMUR Pungky SWK; Vivin Andriani; Diah Karunia Binawati; Sukarjati; Arif Yachya; Dyah Hariani; Susie Amilah
Jurnal Penamas Adi Buana Vol 6 No 02 (2023): Jurnal Penamas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/penamas.vol6.no02.a6427

Abstract

The Cultivation of high-density fish requires artificial feed with high protein content for growth. The higher the protein contained in the feed, the more expensive the feed price, while the feed is the largest cost reaching 60-70% of the production cost. In addition to the problem of feed in high-stocking density fish farming, of course, the waste disposal is also getting higher, which causes failure in the cultivation business. To overcome these problems, it is necessary to make a breakthrough in the cultivation system, one of which is catfish farming using a biofloc system by utilizing heterotrophic bacteria proven to reduce ammonia waste discharge in ponds. The results of this study need to be immediately socialized through training activities for high school teachers. The pretest and post-test results of trainees based on the percentage of activity objectives showed an increase with an average of 55%. It is hoped that the results of the training for high school biology teachers will not only get new knowledge about the application of biofloc to catfish farming. The knowledge gained can be disseminated to students in their schools to support better biology learning
Leaf and Petiole Extract of Centella Asiatica are Potential for Antifertility and Antimicrobial Material Amilah, Susie; Sukarjati, Sukarjati; Rachmatin, Diaz P; Masruroh, Masruroh
Folia Medica Indonesiana Vol. 55, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Ethanol extract of leaves and petiole of pegagan (Centella asiatica) is potential as antimicrobial and antifertility. This study was to investigate whether ethanol extracts of leaf and petiole of Centella asiatica has potential as an antimicrobial and can decrease sperm quality and spermatogenic cells of mice. This was a randomized controlled study using Centella asiatica plant from which we made ethanol extract. The extract of the leaves and petiole of Centella asiatica was subjected to antimicrobial test using the bacteria S. aureus, S. epidermidis, and A. aerogenes. Then the observation was done after a 24-hour-old bacterial cultures by measuring the diameter of the clear zone in mm. Thereafter, the extract was given to randomly grouped mice that were divided into two groups receiving the leaf extract in one group and the petiole exract in another for 35 days. On day 36 the mice were sacrificed for spermatozoa quality and testicular histology examination. Statistical analyses used in this study were ANOVA and LSD tests. There was inhibition zone difference between leaf and petiole (p=0.000). Inhibition zone between bacteria S. epidermidis, S. aureus and A. aerogenes showed no difference (p=0.198). Inter-dose inhibition zone showed difference (p=0.000). Centella asiatica leaf extract showed inhibition zone better than that of Centella asiatica petiole extract. In spermatozoa quality test, there was effect of Centella asiatica leaf extract on sperm motility (p<0.05), viability (p<0.05), morphology (p<0.05) and concentration (p<0.05). No effect of pegagan petiole extract was found on sperm motility (p<0.05), viability (p<0.05), abnormal morphology (p<0.05) and concentration (p<0.05). Highest degradation of spermatozoa quality was obtained from Centella asiatica petiole extract at a concentration of 150 mg/kg bw. Spermatogenic cell test results showed no effect of Centella asiatica leaf extract in decreasing spermatogonia (p=0.000), spermatocytes (p=0.000), and spermatids (p=0.000) counts. No effect of pegagan petiole extract in decreasing the number of spermatogonia, spermatocytes and spermatids. The highest reduction spermatogenic cells of seminiferous tubules was obtained by Centella asiatica petiole extract in a concentration of 150 mg/kg bw. In conclusion: Centella asiatica leaf and petiole extract has antimicrobial potency as contra-ceptives
Role of immunoglobulin g (igg) from the induction of escherichia coli pili adhesion protein isolated from infertile male semen with 32.2 kda molecular weight as opsonin and anti-adhesion an in vitro escherichia coli infection Sukarjati, Sukarjati; Amilah, Susie
Folia Medica Indonesiana Vol. 53, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Escherichia coli (E. coli), a major cause of male genital tract infections, is asymptomatic and may result in male infertility. We have succeeded in isolating and characterizing proteins of E. Pili coli isolates from semen of infertile men who function as adhesin with a molecular weight (MW) 32.2 kDa. This study aims to prove the ability of IgG results adhesion proteins induced pili of E. MW coli 32.2 kDa as opsonin to determine the value of the activity and phagocytic capacity and as an anti- adhesion by calculating the average number of E. coli that attached to human spermatozoa. E. coli infertile men's semen were cultured using standard bacteriology. Peritoneal macrophages were isolated from mice. Spermatozoa from donors were prepared using Sil with Select Plus. IgG was obtained from mice immunized with (1) PBS (control), (2) E. coli pili adhesion protein isolated from infertile men semen with MW of 32.2 kDa and (3) weakened E. coli isolated from infertile men's semen. Phagocytic activity value was determined by counting the number of cells activated macrophage phagocytosis process in 100 cells. Phagocytic capacity value was determined by counting the number of bacteria ingested by 25 macrophages. Anti-adhesion test was done by counting the number of bacteria attached to 100 spermatozoa. The results of this study showed difference (p=0.000) in phagocytic activity and phagocytic capacity (p=0.000) between treatment (1) and (2), and between treatment (1) to (3). However, treatment (2) and (3) did not differ neither in phagocytic activity (p=0.693) nor in phagocytosis capacity (p=0.125). Anti-adhesion test produces difference (p=0.000) in the number of E. coli that attached to human spermatozoa between treatments (1) and treatment (2), and between treatments (1) and (3). The number of E. coli that attached to human spermatozoa between treatment (2) and treatment (3) was not significantly different (p=0.371). In conclusion, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa can increase phagocytic activity and capacity as well as serve as an anti- adhesion. Thus, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa is protective against in vitro E. coli infection, so that it can be used as material to prevent male reproductive tract infections due to E. coli.
Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System Sukarjati, Sukarjati; Amilah, Susie; Sudjarwo, Sudjarwo
Folia Medica Indonesiana Vol. 54, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Escherichia coli (E. coli) is the leading cause of male genital tract infection with no symptoms of infertility. Protein E. coli pili hemagglutinin isolated from infertile male sperm with 32.2 kDa MW acts as adhesion in spermatozoa. This study aimed to prove whether E. coli pili adhesin 32.2 kDa MW is toxic to male reproductive system. Samples consisted of spermatozoa of 30 guinea pigs divided into three groups: control, immunized with E. coli pili adhesin 32.2 kDa MW protein, and transurethral infected E. coli. Observations of sperm motility, vitality and morphology were performed under a microscope. MDA levels and sperm DNA damage were measured by a spectrophotometer and comet assay method and observed using a fluorescent microscope. There was no difference between control and immunization group of E. coli pili adhesin in motility (p=0.499), vitality (p=0.817) and morphology (p=0.176); between control and transuretral infection groups in motility (p=0.000), vitality (p=0.000) and morphology (p=0.000); and between control and both treatment groups in motility (p=0.001), vitality (p=0,000) and morphology (p=0.000). Histologic analysis showed E. coli pili adhesin of 32.2 kDa MW immunization group did not suffer from testicular tissue damage, while the positive group showed a deterioration of seminiferous tubular cells. MDA levels differed between immunization group E. coli pili, transurethral infection group, and control (p=0.024) and between transurethral and control (p=0.007) groups. However, between control and immunized group with E. coli pili protein showed no difference (p=0.251). DNA damage differed (p=0.000) between immunized group with E. coli pili, transurethral infection and control group; between control and transurethral infected group (p=0.000); and between transurethral infection group and E. coli pili protein immunization group (p=0.000). However, between control and E. coli pili immunization group showed no difference (p=0.600). In conclusion, E. coli pili adhesin 32.2 kDa MW protein is not toxic for sperm quality and the quality of sperm molecules.