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All Journal Naturalis : Jurnal Penelitian Pengelolaan Sumberdaya Alam dan Lingkungan TERRA : Journal of Land Restoration AGRITROPICA : Journal of Agricultural Sciences Akta Agrosia
Marulak Simarmata
Agronomy Department, University of Bengkulu, Bengkulu 38371, Indonesia
Agriculture, Biological Sciences & Forestry

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Journal : Akta Agrosia

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Identifikasi Genetik Kultivar Padi Gogo dengan Menggunakan Marka RAPD Simarmata, Marulak; Rustikawati, Rustikawati
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

A research was conducted to identify the genetics of upland rice cultivars using random amplified polimorphic DNA (RAPD) markers. Five out of 60 random primers that capable to amplify the most number of markers were OPE-07 (AGATGCAGCC), OPE-15 (ACGCACAACG), OPH-13 (GACGCCACAC), OPH19 (CTGACCAGCC), and OPM-02 (ACAACGCCTC). The number of markers amplified were 5, 6, 8, 8, and 7, respectively. RAPD-PCR using these 5 primers arranged to DNA templates of 41 rice cultivars amplified 1127 RAPD markers. Polymorphic information content (PIC) of each primer were 0.79, 0.80, 0.87, 0.87, and 0.72, respectively. Cluster analysis using a UPGMA dendogram showed that the 41 cultivars were grouped into 9 clusters with genetic similarity index more than 90 percent.
Penentuan Kadar Garam Kultur Hara untuk Seleksi Toleransi Salinitas pada Padi Lokal Bengkulu Rustikawati, Rustikawati; Simarmata, Marulak; Turmudi, Edhi; Herison, Catur
Akta Agrosia Vol 17 No 2 (2014)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Bengkulu province area elevating from 0 m to over 1000 m above sea level possesses high rice germplasm diversity. To obtain salinity controlling gene(s) from those germplasm, it is required to determine a suitableprotocol. This research was objected to determine NaCl concentration to select Bengkulu rice landraces. The standard most salinity tolerant genotype used in this research was ’Kuning’, a local variety usually grown on tidal area of Seluma Regency for generations. The growing media was Yoshida nutrient culture supplemented with a series of NaCl concentration of 0, 2000, 4000, 6000, 8000 or 10000 ppm. Observation was done every 2 days to find out the response pattern of rice seedling growth on different NaCl concentration. NaCl stress level was determined at 90% seedling dead (LC90) at the eighth day. The results showed that the increase of plantheight was deceased at concentration of 6000 ppm. Based on the mathematical equation generated from the percent mortality data distribution, the LC90 was at 3910 ppm NaCl concentration.
The Growth and Yield of Sweet Corn under Minimum Tillage Technology by Using Various Doses of Herbicide Mixtures of Glyphosate and 2,4-D Simanuhuruk, Bilman Wilman; Simarmata, Marulak; Aprianto, Ayyi
Akta Agrosia Vol 22 No 2 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Glyphosate is a widely used herbicide for minimum tillage technology because it effectively kills broadleaf and grass weeds. Mixing glyphosate with other herbicide that has a different mechanism, such as 2,4-D may increase control efficacy and prevent the evolved of resistant weeds.  Research was conducted to obtain the effective dose of glyphosate and 2,4-D mixtures and to evaluate the effect of the herbicide mixtures on the growth rate and yield of sweet corn. Depression on summed dominance ratios (SDR) and increased number of dominant weeds were observed after the application of glyphosate and 2,4-d mixtures. The interaction of the herbicide mixtures was observed optimum at the doses of glyphosate and 2,4-D of 1.94 and 1.5 L ha-1, respectively, with maximum of net assimilation rates (NAR) at 4-6 weeks after planting of 0.0003617 g cm-2 days-1.  Plant growth rates (PGR) and NAR were observed maximum by a single treatment of glyphosate at a dose of 1.5 L ha-1 which were 2.23614 and 2.23607813 g cm-2 days-1, respectively.  Yield of sweet corn observed as fresh-weight of cobs was found maximum of 129.41 g with a single treatment of glyphosate at 2,018 L ha-1.
Population Characteristic and Genetic Relationship on 25 Bengkulu Heirloom Rice Based on Morpgological Traits Rustikawati, Rustikawati; Simarmata, Marulak; Pujiwati, Hesti; Herison, Catur
Akta Agrosia Vol 22 No 2 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Bengkulu Province has many local rice germplasms that have adapted to specific areas. The germplasm has certain character advantages so that it is maintained by farmers. The characteristics of each germplasm need to be identified in order to improve the properties of the existing germplasm. This study aimed to determine the performance, to estimate the value of genetic parameters, and to determine the genetic relationship of local rice genotypes collected from four districts in Bengkulu Province. The results showed that the ‘Kuning Sulaowangi’ genotype was the earliest in maturity had the highest number of tillers amongst genotypes. Genotypes which have significantly the highest average component yields were ‘Kunig Air Dingin’ and ‘Impera’.  Traits that have a high heritability estimated value were weight of 100 grains (91.046%) and grain weight per panicle (80.252%). The weight of 100 grains also has the highest genetic variability value. Thus increasing local rice production is possible. Based on the results of the analysis with the similarity coefficient of 55%, the local rice genotypes could be grouped into two groups.  Group I consisted of 9 genotypes namely ‘Kuning Pendek’, ‘Humbur’, ‘Cantik’, ‘Pandak Kelabu’, ‘Cisadane Putih’, ‘Kuning Sulaowangi’, ‘China Abang’, ‘China Putih’ and ‘Impera’. Other genotypes were in Group II.  Trait improvement can be done through hybridization between individuals of different groups. To develope early in maturity and high-yielding varieties can be done through hybridization of the ‘Kunig Sulaowangi’ and ‘Kunig Air Dingin’
Co-Authors Agus Martono Angraini, Dila Aprianto, Ayyi Aulia, Ewa Barchia, M. Faiz Barchia, M.Faiz Bieng Brata Clareza, Yessy Diputra, Opila Harta Dwi Wahyuni Ganefianti, Dwi Wahyuni EDHI TURMUDI Heri Dwi Putranto Herison, Catur HESTI PUJIwATI Maemunah Maemunah Mimi Sutrawati Mustopa Romdhon, Mustopa Rahman, Habibur Reftogustadinata, Fitrial Rustikawati Rustikawati Rustikawati Simanuhuruk, Bilman Wilman