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All Journal Jurnal Perikanan Universitas Gadjah Mada Journal of Aquaculture Science Journal of Aquaculture and Fish Health Kontribusia : Research Dissemination for Community Development Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Journal of Marine and Coastal Science Journal of Fisheries & Marine
Rahayu Kusdarwati
Airlangga University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences

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The Effect of Coconut Shell Liquid Smoke (Cocos nucifera) Fish on Nile Tilapia Hematology (Oreochromis niloticus) Tested the Challenge Aeromonas hydrophila Rozi Rozi; Roy C. Prijantono; Sudarno; Rahayu Kusdarwati
Journal of Aquaculture Science Vol 7 No 1 (2022): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v7i1.236

Abstract

Aeromonas hydrophila adalah penyakit bakteri pathogen yang berperan penting dalam menyebabkan kematian pada komoditas ikan nila. Pada penelitian ini, pakan yang mengandung asap cair tempurung kelapa yang dapat diaplikasikan sebagai imunostimulan untuk mengobati penyakit Motile Aeromonad Sepricemia (MAS) pada ikan Nila. Penelitian ini bertujuan untuk mengetahui pengaruh asap cair tempurung kelapa pada uji aktivitas antibakteri secara In vitro dan uji tantang A. hydrophyla secara uji In vivo. Tahapan uji invitro meliputi uji MIC, uji sensitivitas asap cair, dan antibiotik ampicillin sebagai kontrol. Selanjutnya pada uji invivo dengan mencampurkan setiap 100gr pakan yang mengandung asap cair dengan dosis 1%, 3%, 5%, 7% dan 9% (v/w). Selanjutnya uji tantang tantang dilakukan dengan menyuntikan suspensi bakteri A. hydrophila dengan dosis 106 sel/cfu sebanyak 0,1 ml secara oral pada pakan dan variabel pengamatan meliputi total eritrosit, hemoglobin, kadar hematokrit dan gejala klinis ikan nila. Analisis data menggunakan Anova dan diuji DMRT dengan selang kepercayaan 95%. Hasil penelitian menunjukan bahwa konsentrasi asap cair terbaik secara invitro dengan MIC 1% dan sensitivitas asap cair 9% dengan diameter 5.63 cm, sedangkan uji tantang yang paling efektif pada perlakuan 3% dapat menurunkan infeksi A. hydrophyla pada ikan nila dengan jumlah total eritrosit (106/L): 2,27±0,15, total hemoglobin (g/dL): 8,03±0,21, dan kadar hematokrit (%): 21,35±1.34 (p < 0.05). Ikan nila yang diuji tantang dengan A. hydrophila pada perlakuan kontrol menunjukkan gejala sirip geripis, sisik mengelupas, hemoragic, dan exopthalmia. Sedangkan perlakuan yang diberikan asap cair melalui pakan menunjukan kondisi ikan nila lebih baik pada gejala klinis dan analisis hematologi daripada ikan yang tidak diberikan perlakuan.
Development of Entrepreneurial Spirit and Women Empowerment at Pesantren Griya Khadijah Surabaya through Diversification Training on Fishery Products as Food Commodities with High Nutritional Value Putri Desi Wulan Sari; Luthfiana Aprilianita Sari; Rahayu Kusdarwati
Kontribusia : Research Dissemination for Community Development Vol 5 No 1 (2022): KONTRIBUSIA
Publisher : OJS Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.763 KB) | DOI: 10.30587/kontribusia.v5i1.2965

Abstract

The development of an entrepreneurial spirit can be started even with minimal capital. Fishery products with high nutritional value are one of the opportunities that can be developed by community groups as independent businesses in the context of increasing the economy. Santriwati at the Griya Khadijah Islamic Boarding School Surabaya is a national asset that has great potential, role, and opportunity in influencing the social and economic life of the surrounding community. This community service activity aims to improve the competence of female students at the Griya Khadijah Islamic Boarding School, as a role model for hafidzah-preneur education, to be able to develop an entrepreneurial spirit and reflect women's empowerement with good character.
Antibacterial Activity of Crude Extract Red Algae Eucheuma spinosum Against Staphylococcus epidermidis Bacteria Growth Nindhita Yusvantika; Rahayu Kusdarwati; Laksmi Sulmartiwi
Journal of Marine and Coastal Science Vol. 11 No. 3 (2022): SEPTEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v11i3.38286

Abstract

The use of seaweed (Eucheuma spinosum) as a source of bioactive ingredients from algae has not been widely carried out, even though it has secondary metabolites that are thought to have antibacterial activity. The purpose of this study was to determine the inhibitory activity of the crude extract of red algae E. spinosum on the growth of Staphyloccus epidermidis bacteria in vitro. The working method used is an experimental and qualitative research design. Extraction of E. spinosum used maceration with 96% ethanol solvent and evaporated using a rotary evaporator to obtain a crude extract yield of 1.54%. The phytochemical screening test used thin layer chromatography and precipitation tests. The results of phytochemical screening showed that crude extract of Eucheuma spinosum contained alkaloids, flavonoids, terpenoids and saponins. These compounds are thought to have the potential to have antibacterial activity. The antibacterial test was based on a comparative study of the literature using the disc diffusion method (Kirby-Bauer test) with a positive control of Clindamycin 10μl/ml and a negative control of distilled water. Comparison of the literature on the results of the antibacterial activity test is thought to be effective for treating acne-causing bacteria, namely Staphylococcus epidermidis with an average inhibition of 26.5 mm (30 μl). The antibacterial activity is because seaweed contains secondary metabolites such as flavonoids, alkaloids, terpenoids, and saponins. The need for herbal therapy to treat diseases caused by Staphylococcus epidermidis bacteria to reduce bacterial resistance to antibiotics.
Pengaruh Perbandingan Konsentrasi Rumput Laut Terhadap Total Bakteri dan Ragi Kombucha (Kappaphycus Alvarezii) Selama Proses Produksi Rikky Leonard Rikky; Dwitha Nirmala; Rahayu Kusdarwati; Pamela
Journal of Aquaculture Science Vol 8 No 1 (2023): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v8i1.261

Abstract

The comparison of the concentration of Kappaphycus alvarezii seaweed is estimated to affect the total bacteria and yeast during the kombucha production process. The carbohydrate content in seaweed can be one of the factors for the formation of nata as a parameter for the success of making kombucha, as well as its effect on nutrition for bacterial and yeast growth. This study aims to determine the effect of comparing the concentration of seaweed on the total bacteria and yeast kombucha Kappaphycus alvarezii. The method used in this study is an experimental method with a completely randomized design (CRD) with six treatments and 4 replications. The results of this study indicate that the comparison of the concentration of Kappaphycus alvarezii seaweed affects the total bacteria and yeast during the kombucha making process. The total bacteria test showed that the total bacteria increased until the 3rd day and the highest total yield was found in the control treatment, which was 1,6x107 CFU/ml on the 3rd day. Then the total yeast also increased until the 3rd day and the highest yield was found in the control treatment of 2,3x107 CFU/ml.
Utilizing immunostimulants and probiotics for enhancing vannamei shrimp (Litopenaeus vannamei) aquaculture activities Syifania Hanifah Samara; Gunanti Mahasri; Rahayu Kusdarwati; Dheani Nur Ahadya Syifa Chaerunissa; Anggun Khoirun Nikmah; Elisabeth Benita Indarmastuti
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol 8, No 4 (2023): November 2023
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v8i4.11748

Abstract

Vannamei shrimp is one of the important fisheries commodities in Indonesia. This is because vannamei shrimp are expensive, popularly consumed by people around the world, and can be cultured. However, vannamei aquaculture activities are still hampered by disease, less production, and lack of technology implementation. Kedung Pandan Village (Jabon, Sidoarjo, East Java) is one of the potential areas for v vannamei shrimp aquaculture which is also constrained by this issues, so many farmers have gone out of business. This community service activity aims to increase the productivity of vannamei shrimp cultivation by administering immunostimulants and probiotics. In its implementation, this community service activity collaborates with "Putri Mandiri", a group of vaname shrimp farmers in Kedung Pandan Village. This activity is carried out in the stages of preparation, implementation of activities (socialization-demonstration), and evaluation.  Socialization and demonstration activities were carried out on August 9 2023 and were enthusiastically participated by residents. The results of this activity indicate that the knowledge of farmers in Kedung Pandan Village, Jabon, Sidoarjo has increased and can use immunostimulants and probiotics in vannamei shrimp farming activities.
Isolasi dan Identifikasi Bakteri Gram Negatif Pada Luka Ikan Maskoki (Carassius auratus) Akibat Infestasi Ektoparasit Argulus sp. [Isolation and Identification Gram Negative Bacteria At Lessions Of Gold Fish (Carassius auratus) By Infestation Ectoparasite Argulus sp. Kismiyati Kismiyati; Sri Subekti; R. Wahid Nur Yusuf; Rahayu Kusdarwati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 2 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i2.11678

Abstract

AbstractOne of them is the problem of disease Argulus sp. is ektoparasite which often attacked in activity of good conducting of sea water and also freshwater. Argulus sp. attacking at part of fin, husk, gill and entire all its body exterior of him. attacked by fish is Argulus sp. will seen is thin, often rub body and arise red pock which later can generate secondary infection. Utilize to prove the existence of bacterium which emerge effect of lession (secondary infection) which because of bite of Argulus sp. that is by identifying bacterium and biochemical test. Media which used in identifying bacterium for example that is TSA (Tryptic Soy Agar), TSIA (Triple Soy Iron Agar), MIO (Motility Indol Ornithin), O/F medium (Oksidative/Fermentative), sugar test medium (maltosa, laktosa, arabinosa, inositol, manitol, sukrosa). Target of this research is to know the existence of gram negative bacterium and gram negative bacterium any kind of which is found at fish hurt of gold fish (Carassius auratus) effect of infestation by ectoparasite Argulus sp. Research which is use descriptive method. That is with infestation of Argulus sp. into containing aquarium of gold fish counted 1 tail and Argulus sp. counted 5, 10, 15, 20 tail. After Argulus sp. patch all at gold fish is later then perceived until gold fish arise lession effect of bite of Argulus sp. is afterwards identifyed by bacterium to know the bacterium that were formed on gold fish lesions. The result showed that the lesions of gold fish, isolation at TSA medium and identified by biochemical test to diagnose bacteriums found on gold fish lession. Identifications of the three gram negative bacterium as follow : Aeromonas hydrophilla, Pseudomonas flourescens, Flexibacter columnaris while for the Edwardsiella tarda was not be found in this study
Pengaruh Konsentrasi Kadmium Terhadap Perubahan warna Dan Persentase Jenis Kelamin Jantan Anakan Daphnia magna [Influences Concentration Of Cadmium To Colour Change And Male Sex Percentage Neonates Daphnia magna] Rahayu Kusdarwati; Alfi Hermawati W; Setyawati Sigit; A. Shofy Mubarak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11697

Abstract

Abstract Cadmium is one of dangerous heavy metal and it can cause water pollution. As an alternative early warning toward heavy metal in water, Daphnia magna can be used as biology indicator water pollution that caused by heavy metal. Daphnia magna is organism that ussualy used for bioassay in many progressing countries, because that organism have important role in freshwater ecology, short life cycle about 3 weeks and sensitive toward chemical environment. Grade of toxicity heavy metal can measured with lethal and sub lethal parameter. In Daphnia magna, sub lethal parameter that can be observe are colour changes and male sex percentage neonates. The purpose of this research was to know the colour changes and male sex percentage neonates Daphnia magna in different concentration of cadmium. Target of this research is to gets information about level colour changes and male sex percentage neonates Daphnia magna in different concentration of cadmium. The main parameter was colour changes and male sex percentage neonates and the secondary parameters were water quality (temperature, pH, dissolved oxygen and NH3) The result of research of giving different cadmium concentration to the adult female Daphnia magna not showing significant influence, nevertheless it is showing significant influence toward male sex percentage neonates Daphnia magna. The treatment with cadmium concentration 0,0004 mg/l produce 100% male neonates Daphnia magna. Water quality during research were in optimal conditions to support Daphnia magna life, those are temperature 26°C, pH range from 8,0 – 8,2 and dissolve oxygen (DO) range from 8,5 – 9,0 mg/l and ammonia 0 – 0,03 mg/l.
Co-Authors A. Shofy Mubarak Aldiansyah Budi Wardana Alfi Hermawati W Andriana Kusuma Wardhani Anggun Khoirun Nikmah Ayu Puspitarani Dahlia Dahlia Dandi Pradana Dheani Nur Ahadya Syifa Chaerunissa Dwitha Nirmala Elisabeth Benita Indarmastuti Gunanti Mahasri Hari Suprapto Kismiyati , Laksmi Sulmartiwi Linnya Prima Agustin Luthfiana Aprilianita Sari Muhamad Amin Nindhita Yusvantika Pamela Pipin Tri Anjani R. Wahid Nur Yusuf Rikky Leonard Rikky Roy C. Prijantono Rozi Rozi Samara, Syifania Hanifah Sari, Putri Desi Wulan Setyawati Sigit Sri Subekti Sudarno Sudarno Sudarno Sumini Sumini Woro Hastuti Setyantini Yudha Teguh Prayogi