Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Jurnal Natur Indonesia JURNAL TEKNOLOGI LINGKUNGAN Media Penelitian dan Pengembangan Kesehatan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Kimia Terapan Indonesia Bionature
Yati Sudaryati Soeka
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Materials Science & Nanotechnology Public Health

Published : 23 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 23 Documents
Search

 1   2   3 
SEMI PURIFIKASI DAN UJI REAKSI TRANSGLUKOSILASI P-GLUKOSIDASE DARI ASPERGILLUS PULVERULENTUS Sulistyo, Joko; Soeka, Yati Sudaryati; Dewi, Purnama
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1263

Abstract

An extracellular p-glucosidase[EC 3.2.1.21]derived from Asvereillus vulverulentus were separated and partially purified by successive chromatographies and its some characterization and transglucosylation capacity were studied.The purification protocol included precipitation with ammonium sulphate,gel filtration,ion exchange chromatography and native polyacrylamide gel electrophoresis.The enzyme readily hydrolysed cellobiose to form transglucosylation products in the present of primary alcohol acceptors.This P-glucosidase was stable at temperatures up to 70 °C and from pH 2.5 to 8.5, while its highest activity was in the pH 4.5 at 65°C.
KARAKTERISASI PROTEASE BACILLUS SUBTILIS A1 INACC B398 YANG DIISOLASI DARI TERASI SAMARINDA Soeka, Yati Sudaryati; Sulistiani, Sulistiani
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.694

Abstract

Proteases is enzyme that breaks the peptide bond to produce amino acids and simpler peptides. This enzyme can be isolated from a variety of sources such as plants, animals and microbe. Alkaline proteases of microbial origin possess considerable industrial potential due to their biochemical diversity and wide applications in tannery, food, medicinal formulations and detergents. The objectives of the research was to determine the characteristics of the protease enzyme produced by strain A1, including incubation time, substrate concentration azokasein, the optimum temperature and pH also stability. The effect of some metal ions as activators or inhibitors of the protease enzyme activity measured with a spectrophotometer at ? 280 nm. Strain A1 was identified by using 16S rDNA sequencing and phylogenetic analysis based on Neighbor Joining method. Strain A1 protease activity was qualitatively demonstrated the presence of a clear zone around the colonies in the medium containing 1% skim milk. Result showed that the highest activity were incubation time of three days, temperature of 50 ºC and pH 8.5 were 87.35 U/mL, 83.44 U/mL and 93.11 U/mL, respectively. Effect of metal ions in the form of divalent and monovalent cations at a concentration of 1 mM on protease A1 activated by divalent cations CaCl2, MnCl2 while divalent cations CuCl2, HgCl2 and monovalent cations KCl, NaCl were inhibitors of each enzyme activity. Result from molecular identification based on 16S rDNA sequence and phylogenetic analysis using Neighbor Joining method suggested that strain A1 was Bacillus subtilis. The strain was registered in the InaCC collection (no. B 398).
AKTIVITAS AKTINOMISETES DARI BANGKA-BELITUNG KOLEKSI BIDANG MIKROBIOLOGI, PUSLIT BIOLOGI- LIPI DALAM MEMPRODUKSI ENZIM KITINASE Soeka, yati Sudaryati; Triana, Evi; Setianingrum, Ninu
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.075 KB) | DOI: 10.29122/jtl.v11i3.1187

Abstract

The aim of the research was to know the capability of actinomycetes isolate from Bangka Belitung, which stored at Microbiology-LIPI Culture Collection, in producing chitinase enzyme. This isolate which could produce chitinolitic enzyme, signed by clear zone at medium contain 1% chitine. The chitinase activity of the isolate which incubated for 1-7 days in the room temperature was analyzed by spectrophotometer in λ 584 nm. The result of this experiment was highest chitinase activities with incubated for 3 days, were 1.66 . 10-2 U/ml. Maximum chitinase activities was found at 1% starch soluble substrate 2.83 . 10-2, pH 8.0 and at 50°C condition were 9.3 . 10-2 and 12.98 .10-2 U/ml respectively.Key words : chitinase, clear zone, spectrophotometer
BIOTRANSFORMASI PIROKATEKOL GLIKOSIDA MENGGUNAKAN KULTUR SUSPENSI SEL SOLANUM MAMMOSUM L. Soeka, Yati Sudaryati; Sulistyo, Joko
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.01 KB) | DOI: 10.24002/biota.v15i2.2704

Abstract

A syntesis of pyrocathecol glucoside was carried out by applying biotransformation cell culture suspension from calus of Solanum mammosum L., on modified medium of Murashige and Skoog (MS). A growth maximum volume at 15,5 ml of cell culture suspension of S. mommosum was achieved on day-8 incubation. The results showed that pyrocathecol glucoside as a bitransformation product that was obtained by application of Pyrocathecol at 50-200 ppm was determined by TLC and identified at Rf value of 0.82?0.83. Futhermore, the biotransformation products were determined by HPLC obtained from the cell culture suspension at concentration of 200 ppm pyrocathecol so that resulted in reaction products based on standard solution. The peaks number 1, 2 and 3 with retention time 2.53 min, 4.62 min and 7.58 min were appropriate to the retention time cellobiose, glucose and methyl ?-glucoside, respectively. Peak number 4 with retention time 8.52 min conformed to pyrocathecol-glucobioside as a product of side transfer and peak number 5 with retention time 10.52 min in line with the retention time of arbutin were pyrocathecol-glucoside as a transfer product expected from the result of biotransformation.
BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED POLYPHENOL GLYCOSIDE ON MICROBIAL GROWTH Soeka, Yati Sudaryati; Widiasih, Lutfi Erlita; Sulistyo, Joko
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3467

Abstract

ABSTRACTBiological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth. We have studied an indigenous bacterial strain produced a glycosyl transfer enzyme (CGTase) yielding polyphenol glycosides from a substrate of starch and polyphenol-aglycone. We observed that the CGTase derived from culture filtrate of some microbial strains (Candida rugosa, Bacillus megaterium, B. coagulans and B. polymixa) could synthesize transfer products in the presence of appropriate polyphenol-aglycones as their acceptors. An inhibitory effects of enzymatically synthesized polyphenol glycosides against bacterial growth was furthermore examined. It was found that polyphenol-glycoside, as one of the transfer products, exhibited high antibacterial activity on the growth of Bacillus subtilis and Escherichia coli, no effect when on Bacillus cereus.Key words : Cyclodextrin glucano transferase (CGTase), enzymatic transglycosylation,polyphenol glycoside, antibacterial activity.
OPTIMASI ENZIM ?-AMILASE DARI BACILLUS AMYLOLIQUEFACIENS O1 YANG DIINDUKSI SUBSTRAT DEDAK PADI DAN KARBOKSIMETILSELULOSA Soeka, Yati Sudaryati; Rahmansyah, Maman; ., Sulistiani
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2200

Abstract

 ABSTRACTBacterial code O1 had been isolated from the leaven of fermented cassava. Based on molecular analysis by partial sequences of 16S rDNA and the phylogenetic character interpretation with Neighbor Joining Method, the strain was identified as Bacillus amyloliquefaciens O1. Bacterial enzymatic activity of ?-amylase was clarified due to the affect of temperature and pH, and as well as its enzymatic stability to convert 2% soluble starch in 100 ml standard media. Aim of the study was to provide benefit in regard on ?-amylase application as crude enzyme extract from the bacteria. In this study, the bacterial strain was being activated to produce ?-amylase by modifying substrates containing cassava starch, rice bran (RB), and carboxymethylcellulose (CMC) in five times volumes (500 mL) of the first scale setting in the standard media.  The result, reducing sugar as a result of enzymatic activity process increased 40 and 55 times in the modified media containing RB and CMC, respectively after 24 hours incubation. In the next 24 hours observation, enzyme activity in bacterial culture based on the RB media was able to degrade amylum in the muslin material containing amylum which was plunged in the media, 1.23 times higher compared to bacterial culture based on the CMC media. Media formula used in the study was able to induce extracellular enzyme activity as well as bacterial culture growth. Keywords: ?-amylase, Bacillus amiloliquefaciens, rice bran, carboxymethylcellulose 
PROFIL VITAMIN, KALSIUM, ASAM AMINO DAN ASAM LEMAK TEPUNG JEWAWUT (SETARIA ITALICA L.) FERMENTASI Soeka, Yati Sudaryati; Sulistiani, Sulistiani
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3098

Abstract

ABSTRACTFoxtail millet (Setaria italica L.) is tropical cereal grains of Poaceae. Foxtail millet starch content is quite high, so it has the potential to be used as food raw material; This study has been conducted by making foxtail millet flour fermented with starter bacteria of cellulolytic and amylolytic Bacillus amyloliquifaciens B7 and lactic acid bacteria of Lactobacillus plantarum SU-LS537 which can degrade phytic acid. Parameters measured in the fermentation of foxtail millet was amount of vitamin E, B6 and B12, calcium, essential and non essential amino acids, essential and non essential fatty acids. Fermented foxtail millet decreased vitamin content. A ten fold increase content of calcium concentrations, essential amino acids (histidine, threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysis), non-essential amino acids (aspartic acid, glutamic acid, serine, glycine, arginine, alanine, proline, tyrosine, and cysteine), the fatty acid (lauric , palmitic) and decrease of fatty acid stearic (non essential fatty acids). Bacillus amyloliquifaciens B7 fermentation increased oleic acid but it decreased linoleic acid while Lactobacillus plantarum SU-LS537 fermentation increased linoleic acid, but it decreased oleic acid.Keywords: jewawut (Setaria italica L.), flour, fermentation, Bacillus amyloliquifaciens B7, Lactobacillus plantarum SU-LS537
Aktivitas Antimutagen Isoflavon Glikosida Hasil Transglikosilasi Enzimatik CGT-ase Bacillus macerans Joko Sulistyo; Yati Sudaryati Soeka
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.2629

Abstract

It has been known that isoflavone have biological activities such as antioxidant, antibacteria, antimutagenesis, and anticancer. Isoflavone aglycone uses such as genistein, daidzein and glycitein are limited since they are unstable and uneasily to dissolve in water. Through enzymatic transglycosylation reaction, its stability and solubility could be improved. In this study, genistin (isoflavone glycoside) was synthesized from genistein (isoflavone aglycone) by application of transfer reaction using enzyme cyclodextrin glucanotransferase (CGT-ase) of Bacillus macerans. Identification of the product was determined by TLC with methanol: chloroform (1:3, v:v) as eluent. Rf value 0.75 of the synthesized product was close to the Rf value standard of authentic genistin glycoside. The synthesized genistin was furthermore assayed to determine its antimutagenesis activity according to the Ames methode on E. minimal glucose media had been precultured with a mutant strain of Salmonella thypimurium TA98. The tested bacterial strain was induced with aflatoksin B1 as mutagen which had been activated with a lever homogenate. The result showed that the solubility and some biological properties of the synthesized genistin were improved higher than that of genistein, while it was found to be lower than that of the commercial genistin.
Biotransformasi Pirokatekol Glikosida Menggunakan Kultur Suspensi Sel Solanum mammosum L. Yati Sudaryati Soeka; Joko Sulistyo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2704

Abstract

A syntesis of pyrocathecol glucoside was carried out by applying biotransformation cell culture suspension from calus of Solanum mammosum L., on modified medium of Murashige and Skoog (MS). A growth maximum volume at 15,5 ml of cell culture suspension of S. mommosum was achieved on day-8 incubation. The results showed that pyrocathecol glucoside as a bitransformation product that was obtained by application of Pyrocathecol at 50-200 ppm was determined by TLC and identified at Rf value of 0.82−0.83. Futhermore, the biotransformation products were determined by HPLC obtained from the cell culture suspension at concentration of 200 ppm pyrocathecol so that resulted in reaction products based on standard solution. The peaks number 1, 2 and 3 with retention time 2.53 min, 4.62 min and 7.58 min were appropriate to the retention time cellobiose, glucose and methyl α-glucoside, respectively. Peak number 4 with retention time 8.52 min conformed to pyrocathecol-glucobioside as a product of side transfer and peak number 5 with retention time 10.52 min in line with the retention time of arbutin were pyrocathecol-glucoside as a transfer product expected from the result of biotransformation.
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) Joko Sulistyo; Yati Sudaryati Soeka; Evi Triana; Rostiati NR Napitupulu
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.
Co-Authors Evi Triana Evi Triana Joko Sulistyo Joko Sulistyo Joko Sulistyo Maman Rahmansyah Naiola, Elidar Naiola, Elidar Napitupulu, Rostiati NR Purnama Dewi Purnama Dewi Rahayu, Sri Hartin Rostiati NR Napitupulu Setianingrum, Ninu Sulistiani ., Sulistiani Sulistiani Sulistiani, Sulistiani Widiasih, Lutfi Erlita Widiasih, Lutfi Erlita