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All Journal Jurnal Natur Indonesia JURNAL TEKNOLOGI LINGKUNGAN Media Penelitian dan Pengembangan Kesehatan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Kimia Terapan Indonesia Bionature
Yati Sudaryati Soeka
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Materials Science & Nanotechnology Public Health

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SEMI PURIFIKASI DAN UJI REAKSI TRANSGLUKOSILASI P-GLUKOSIDASE DARI Aspergillus pulverulentus Joko Sulistyo; Yati Sudaryati Soeka; Purnama Dewi
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1263

Abstract

An extracellular p-glucosidase[EC 3.2.1.21]derived from Asvereillus vulverulentus were separated and partially purified by successive chromatographies and its some characterization and transglucosylation capacity were studied.The purification protocol included precipitation with ammonium sulphate,gel filtration,ion exchange chromatography and native polyacrylamide gel electrophoresis.The enzyme readily hydrolysed cellobiose to form transglucosylation products in the present of primary alcohol acceptors.This P-glucosidase was stable at temperatures up to 70 °C and from pH 2.5 to 8.5, while its highest activity was in the pH 4.5 at 65°C.
Pemanfaatan Limbah Kulit Udang untuk Menghasilkan Enzim Kitinase dari Streptomyces macrosporeus InaCC A454 Yati Sudaryati Soeka; Evi Triana
Jurnal Kimia Terapan Indonesia Vol 18, No 01 (2016)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.441 KB) | DOI: 10.14203/jkti.v18i01.43

Abstract

Kitinase (EC 3.2.1.14) adalah enzim yang dapat menghidrolisis ikatanpanjang polimer asetil-D-glukosamina (kitin) menjadi ikatan polimer kitinyang lebih pendek. Kitin banyak ditemukan pada cangkang hewaninvertebrata laut, terutama krustasea,serangga, moluska, dinding sel jamur.Limbah udang sangat penting sebagai sumber kitin. Penelitian ini bertujuanuntuk menentukan karakteristik enzim kitinase yang dihasilkan olehaktinomisetes asal P. Belitung dengan substrat kitin dari limbah udang,meliputi waktu inkubasi, pengaruh pH, suhu optimum serta stabilitas.Pengaruh beberapa ion-ion logam sebagai aktivator atau inhibitor terhadapaktivitas enzim kitinase diukur dengan spektrofotometer pada λ 584 nm.Strain aktinomisetes diidentifikasi secara molekuler berdasarkan 16S rDNAsekuen. Aktivitas kitinase secara kualitatif diperlihatkan dengan adanya zonabening di sekitar koloni pada media yang mengandung 1% koloidal kitin.Hasil menunjukkan bahwa aktivitas tertinggi pada waktu inkubasi lima hari,suhu 50ºC dan pH 8 berturut-turut sebesar 0,0304 U/mL, 0,0312 U/mL dan0,0421 U/mL. Pengaruh ion logam dalam bentuk kationdivalen danmonovalen masing-masing pada konsentrasi 1 mM diaktifkan olehkationdivalen CaCl2, MnCl2, CuCl2 dan kation monovalen KCl, NaCl.Identifikasi molekuler berdasarkan sekuen16S rDNA menunjukkan bahwaisolat aktinomisetes ini adalah Streptomyces macrosporeus. Strain ini telahdidaftarkan di kultur koleksi InaCC dengan nomor koleksi A 454. 
Bioproses Penguraian Limbah Cair Buangan Industri Pangan dan Non-Pangan Berbasis Biosurfaktan Yati Sudaryati Soeka; Joko Sulistyo
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

Biosurfactants are microbial metabolites that may be utilized to replace synthetic chemical surfactant inovercoming the problem of industrial wastewater pollution. Indications of the presence of biosurfactant activitymay be determined through measurement of emulsification and enzymatic activity of the tested microorganismson their growth media, which is indicated by decreasing the BOD and COD of the tested growth media containingwastewater material as an inducer added to the microbial growth medium. This research was conducted toexamine several potential microbial isolates that produce biosurfactants on growth media containing mixed wastewater. The results showed that some of the microbial cultures tested showed the potential to produce biosurfactantsas indicated by the emulsification and enzymatic activity of the tested microbial culture. Trial on mixed industrialwastewater showed that the tested biosurfactants were able to reduce the levels of BOD (440-1300 mg/l) and COD(1240-1760 mg/l) of the wastewater samples up to low BOD levels (82 mg/l) and COD (116 mg/l), respectively,thus that it meets the requirements of the established wastewater quality standards according to the IndonesianStandard regarding with the respective BOD (50-100 mg/l, 06-6989.9-2004 SNI) and COD (100-250 mg /l, 06-6989.2-2004 SNI).
Co-Authors Evi Triana Evi Triana Joko Sulistyo Joko Sulistyo Joko Sulistyo Maman Rahmansyah Naiola, Elidar Naiola, Elidar Napitupulu, Rostiati NR Purnama Dewi Purnama Dewi Rahayu, Sri Hartin Rostiati NR Napitupulu Setianingrum, Ninu Sulistiani ., Sulistiani Sulistiani Sulistiani, Sulistiani Widiasih, Lutfi Erlita Widiasih, Lutfi Erlita