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Profil Kadar Hemoglobin pada Wanita Pre-Eklampsia Berat Dibandingkan dengan Wanita Hamil Normal Tiaranissa, Azhalia; W.B, Siti Candra; Sriwahyuni, Endang
Majalah Kesehatan FKUB Vol 1, No 3 (2014)
Publisher : Faculty of Medicine Universitas Brawijaya

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Abstract

Pre-eklampsia adalah penyakit yang terdiri dari hipertensi dan proteinuria pada wanita hamil yang terjadi mulai minggu ke-20 kehamilan sampai minggu ke-6 setelah melahirkan. Pada wanita pre-eklampsia ditemui kelainan hematologis yaitu gangguan degradasi heme dan hemokonsentrasi yang menyebabkan peningkatan kadar hemoglobin dibandingkan dengan wanita hamil normal. Penelitian ini bertujuan untuk mengetahui profil kadar hemoglobin pada wanita pre-eklampsia berat dibandingkan dengan wanita hamil normal. Penelitian ini merupakan penelitian deskriptif analitik dengan cross sectional di RSSA Malang. Jumlah sampel yang digunakan yaitu 60 sampel wanita hamil dengan usia kehamilan > 20 minggu, 30 wanita pre-eklampsia berat dan 30 wanita hamil normal sebagai kontrol. Pengambilan darah dan pengukuran kadar hemoglobin dilakukan di laboratorium Patologi Klinik RSSA Malang. Hasil uji independent T-test didapatkan nilai signifikan (p = 0.000) yang menunjukkan ada perbedaan yang bermakna pada kadar hemoglobin antara wanita pre-eklampsia berat dengan wanita hamil normal. Rata-rata kadar hemoglobin pada wanita hamil dengan pre-eklampsia berat adalah 13,26 ± 0,95 dan rata-rata kadar hemoglobin pada wanita hamil normal adalah 10,74 ± 1,07. Penelitian ini juga menunjukkan adanya peningkatan kadar hemoglobin pada wanita pre-eklampsia berat. Penelitian ini menyimpulkan bahwa kadar hemoglobin pada wanita pre-eklampsia berat lebih tinggi daripada kadar hemoglobin wanita hamil normal. Kata kunci : Hamil normal, Kadar haemoglobin, Pre-eklampsia berat.
PENGARUH PEMBERIAN EFEK JINTEN HITAM (NIGELLA SATIVA) TERHADAP APOPTOSIS TERHADAP SEL EPITEKL BRONKIOLUS DAN APOPTOSIS LIMFOSIT T SALURAN NAFAS BRONCHIOLUS PADA MODEL MENCIT ASTHMA Sriwahyuni, Endang; NUR ARIFAH, IMAMA KHALIS
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 6, No 2 (2010): Desember 2010
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.22219/sm.v6i2.1060

Abstract

Abstract   Airway remodelling refers to the structural changes that occur in the airway wall in asthma.  These is  irreversible changes to the airway, with damage of bronchiolus epithelium because the  airway epithelium undergoes apoptosis  as one of its manifestations. Empirically black seed has been known as bronchial asthma therapy for many years ago. The purpose of the research is to test the effect of black seed (Nigella sativa L.) extract to apoptosis of bronchiolus epithelium and apoptosis of T lymphocytes the airway bronchial asthma on asthma mouse model. Female mice were used in the expeimental laboratory research and allergic mouse model was got by given ovalbumin twice within 3 weeks intraperitoneally and 3 times per week within 6 weeks by inhalation. Black seed extract is given in 3 different dose (0,024 cc/day, 0,048 cc/day and 0,096 cc/day) for 9 weeks. Sample was chosen randomly to share it within 5 group containing 6 mice in every group ; negative control group (without any treatment, n=6), positive control group (sensitizied with ovalbumin, n=6), JH1 group (sensitizied with ovalbumin and treating with first dose of black seed extract, n=6), JH2 group (sensitizied with ovalbumin and treating with second dose of black seed extract, n=6), JH3 group (sensitizied with ovalbumin and treating with third dose of black seed extract, n=6). The parametric that is used in this research is the change in the color of the nucleus of bronchiolus epithelium and T lymphocytes the airway bronchial asthma shown the occurrence of browning apoptosis. The result shown a significant increase of apoptosis of bronchiolus epithelium on  positive control group to compare with negative control group. The apoptosis of bronchiolus  epithelium on JH1 shown an increase compare with positive control group. For JH2 and JH3 groups shown a decrease the apoptosis of bronchial epithelium compare with positive control group. And the research indicate that the unsignificant aptosis decrease were happen on  a group with positive control instead of the group with negative control. Meanwhile, on JH1 group, apoptosis decrease were happening more significantly than the positive control group does, the aptosis increases were happen on JH2 and JH3 group, but its not capable to exceeding the positive control group apoptosis amount The conclusions is the black seed (Nigella sativa L.) extract can decrease apoptosis of bronchiolus epthelium on asthma mouse model in the third dose as the best result and the doses of black seed extract that were giving to the experiment mice can not increasing the apoptosis T Lymphocyte, thus requiring further research to figure out the perfect doses to increasing against respiratory tract apoptosis T Lymphocyte.   Keyword: asthma, black seed, the apoptosis of bronchiolus epithelium, apoptosis limfosit T.
Pemberian Kombinasi Vitamin C dan E Peroral Memperbaiki Kerusakan Hepar Akibat Paparan Rokok Kretek Sub Kronik Muliartha, I Ketut Gede; Sriwahyuni, Endang; Yuliawati, Yuliawati
Jurnal Kedokteran Brawijaya Vol 25, No 1 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.627 KB) | DOI: 10.21776/ub.jkb.2009.025.01.5

Abstract

ABSTRACTFree radicals in cigarette smoke can destroy tissue and cells of the body. The activity of free radicals can bereduced by antioxidants from external and internal. The objective of this research is to investigate the effectsof vitamin C and vitamin E combination on the histopathology of rat liver exposed by subcronic cigarettesmoke. This research using true experimental study with post test only control group design. The sample othis research we used 32 Wistar rat, ages between 2-3 months, weight 150-200 grams divided 8 groupedCigarette smoke exposed for 10 weeks. Parameter will be measured is the number of liver cells damage(necrosis). Data analysis uses the One-Way ANOVA method followed by Turkey test. The result show thenumber of liver cells damage are significantly decrease. The conclution vitamin C and vitamin E combinationdecrease the number of the liver cells damage of the rat liver, that has been exposed to subcronic cigarettesmoke at optimal doze 0,20 mglg bw vitamin C and 0,04 lUlg bw vitamin E.Keywords: vitamin C, vitamin E, cigarette smoke, liver cells damage.
Stress Sel Imunokompete Mukosa Usus oleh Bahan Aktif Alkil Benzen Sulfonat (ABS) dalam Daterjen Sriwahyuni, Endang
Jurnal Kedokteran Brawijaya Vol 18, No 1 (2002)
Publisher : Fakultas Kedokteran Universitas Brawijaya

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Abstract

Stress Sel Imunokompete Mukosa Usus oleh Bahan Aktif Alkil Benzen Sulfonat (ABS) dalam Daterjen
Ekstrak Jinten Hitam Memperbaiki Penyempitan Jalan Nafas pada Model Mencit Asthma Sriwahyuni, Endang; Risza, Faradina; Yuni, Anita
Jurnal Kedokteran Brawijaya Vol 26, No 1 (2010)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (819.443 KB) | DOI: 10.21776/ub.jkb.2010.026.01.10

Abstract

ABSTRAKRemodeling jalan nafas pada asma merupakan perubahan yang bersifat ireversibel dan di tunjukkan dengan penebalan epitel. Asma merupakan kondisi hipersensitif jalan nafas yang disebabkan inflamasi dan ditunjukkan dengan penyempitan bronkus. Jinten hitam sudah digunakan untuk pengobatan asma sejak lama. Studi dilakukan untuk menguji efek jinten hitam dalam mencegah penebalan bronkus dan membuktikan efek bronkodilator dengan peningkatan lingkar lumen bronkus pada model mencit asma betina dengan eksperimental in vivo. Mencit model asma dikembangkan dengan memberikan sensitisasi allergen ovalbumin yang diberikan intraperitoneal dua kali dalam 3 minggu (haro 0 dan 14) dilakukan per inhalasi tiga kali seminggu selama 6 minggu. Ekstrak jinten hitam diberikan dalam 3 dosis (1,2g; 2,4 g and 4,8 g/kgBB/hari) selama 9 minggu. Hasil penelitian menunjukkan bahwa pemberian jintan hitam dapat mencegah penebalan epitel bronkus dengan dosisi efektif 2,4 g/Kg BB/hari dan meningkatkan lingkar lumen bronchial dengan dosis efektif 4,8 g/KgBB/hari. Dapat disimpulkan pemberian ekstrak jintan mencegah penebalan epital bronkus dan meningkatkan lingkar lumen bronchial pada mencit model asma.Kata Kunci : Asma, inflamasi, jinten hitam, lingkar lumen bronkus, penebalan epitel bronkus
PENGARUH GAYA BERENANG (GAYA BEBAS DAN GAYA DADA) TERHADAP PERUBAHAN VOLUME OKSIGEN MAKSIMUM Dennison, Dennison; Sriwahyuni, Endang; Wardhani, Viera
Jurnal Kedokteran Brawijaya Vol 19, No 2 (2003)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.957 KB) | DOI: 10.21776/ub.jkb.2003.019.02.5

Abstract

ABSTRACT Swimming  is  frequently  used  to  improve  the  individual  physical  endurance.  Most  of  the  studies  have  proved  that  swimming  can improve the maximum oxygen volume effectively, but none of them compare the differences in the swimming styles. The study was aimed to analyse the differences of swimming styles in improving maximum oxygen volume, so it can be used as basis for physical training scheme. A pretest-posttest experimental design was conducted in two sample group  (front crawl stroke group and breast stroke  group)  consists  of  six  people  in  each  group.  The  samples  were  selected  based  on  questionaire  to  control  the  confounding variables, ie: sex, age, height, weight, training habit and health status (anemia, heart disease and respiratory distress). The training method were carried out in 12 session in 4 weeks (3 session per week) by increasing the swimming distance gradually. Each session arrangement was consists of warming up - training in short sets with rests - relaxation. The maximum oxygen volume was measured using Cooper method and the result was analyzed using independent sample t-test with SPSS 11. The result shows that there is a significant difference in maximum oxygen volume improvement  (t= -4.718, CI 95% = lower: -5.014; upper: -1.722, p= 0.002) in which the result in breast stroke group has  a higher improvement (mean: 6.313, sd: 1.378)
Pengaruh Faktor Sosial Ibu terhadap Keberhasilan Menyusui pada Dua Bulan Pertama Amin, Wirawati; Indrawan, I Wayan Agung; Sriwahyuni, Endang
Jurnal Kedokteran Brawijaya Vol 28, No 2 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (536.199 KB) | DOI: 10.21776/ub.jkb.2014.028.02.16

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Tingkat keberhasilan pemberian Air Susu Ibu pada dua bulan pertama masih rendah, padahal masa ini merupakan masa percepatan pertumbuhan pada bayi, disaat kebutuhan bayi meningkat. Penelitian ini bertujuan untuk menganalisis pengaruh faktor sosial ibu meliputi umur, pendidikan, pekerjaan, pengetahuan, Inisiasi Menyusu Dini (IMD), dukungan suami dan teknik menyusui terhadap keberhasilan menyusui pada dua bulan pertama di RSKDIA Pertiwi Makassar. Metode penelitian ini adalah observasional analitik, dengan purposive sampling sebanyak 131 di Rumah Sakit Khusus Daerah Ibu dan Anak Pertiwi Makassar pada Oktober 2013-Januari 2014. Pengukuran teknik menyusui digunakan lembar observasi/check list yang dinilai oleh peneliti sendiri, sedangkan penilaian keberhasilan menyusui dilakukan melalui kunjungan rumah setelah subjek pulang dari Rumah Sakit, yakni pada satu minggu, dua minggu dan delapan minggu setelah subjek melahirkan, dengan menggunakan kuesioner. Analisis hasil penelitian menggunakan uji Chi-Square. Hasil penelitian menemukan ada pengaruh positif antara pendidikan (p=0,006; OR=2,826), pekerjaan (p=0,001; OR=0,293), pengetahuan (p=0,000; OR=14,792), IMD (p=0,000; OR=6,771), dukungan suami (p=0,000; OR=10,988) dan teknik menyusui (p=0,001; OR=3,784) terhadap keberhasilan menyusui pada dua bulan pertama. Hasil penelitian ini menyimpukan bahwa ibu dengan tingkat pendidikan tinggi, tidak bekerja, mempunyai pengetahuan yang baik, melaksanakan IMD, mempunyai dukungan aktif dari suami, memiliki teknik menyusui yang baik dapat meningkatkan keberhasilan menyusui pada dua bulan pertama.Kata Kunci: Air Susu Ibu, faktor sosial ibu, keberhasilan menyusui dua bulan pertama
The Effect of Animal Hunting and Rounders Games on Locomotor and Manipulative Motion Capabilities Febrianto, Faizal Adin; Kartiko, Dwi Cahyo; Sriwahyuni, Endang
International Journal for Educational and Vocational Studies Vol 2, No 1 (2020): January 2020
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/ijevs.v2i1.2037

Abstract

Elementary school-age children are very active so they prefer to play. Before starting the learning activities, the teacher designs a learning model that is interspersed with games so that the child can receive the material well and is not easily bored during the learning process. In other words, the activities carried out by children are more directed to the activities of playing and moving. One game that is loved and often played by children is hunting animals and baseball. The purpose of this study is to analyze: (1) studying and analyzing the effect of the application of animal hunting game learning models on locomotor and manipulative motion abilities. (2) to study and analyze the effect of the application of baseball game learning models to increase locomotor and manipulative motion skills. (3) to find out, study and analyze the effect of learning based on game hunting animals and baseball to increase locomotor and manipulative mobility. This type of research used in this study is quantitative with a research design using a two-group pre-test post-test design, with data analysis using paired sample t-test and independent sample t-test. The results showed: (1) there was an effect of the application of animal hunting games to locomotor and manipulative mobility. (2) there is an effect of the application of baseball games on locomotor and manipulative motion abilities. (3) in an animal hunting game, the locomotor base motion has an increase of 2.17 and manipulative motion has an increase of 5.1. In a baseball game, the locomotor base motion has an increase of 1.5 and manipulative base motion has an increase of 3.77. Based on the above analysis, it can be concluded that there is an increase in locomotor and manipulative movement abilities for each group after being given a game. Also, there are differences in influence between the animal hunting group and the baseball group as seen from the increase in locomotor motion through independent sample t-test where the game of animal hunting gives a better effect than the game of baseball.
Genistein To Decrease Matrix Metalloproteinase-2 (Mmp-2) And Matrix Metalloproteinase-9 (Mmp-9) Levels In Peritoneal Fluid Of Endometriosis On Mice Model Maharani Maharani; Endang Sri wahyuni; Sutrisno Sutrisno
JKM (Jurnal Kebidanan Malahayati) Vol 8, No 1 (2022): Vol. 8 No. 1, Januari 2022
Publisher : Program Studi Kebidanan Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkm.v8i1.5847

Abstract

Latar Belakang: Matrix metalloproteinases (MMPs) mempengaruhi progesivitas endometriosis, dalam invasi, perkembangan dan metastasis endometriosis. Penururan kadar MMP-2 dan MMP-9 merupakan terapi yang tepat untuk kasus endometriosis menggunakan terapi hormonal alami yaitu genistein.Tujuan: Penelitian ini bertujuan untuk mengetahui pemberian genistein dapat menurunkan kadar MMP-2 dan MMP-9 dalam cairan peritoneal mencit (Mus musculus) model endometriosis.Metode: Penelitian ini merupakan penelitian eksperimental yang dilakukan di Laboratorium Fisiologi Reproduksi Embriologi Fakultas Kedokteran Hewan Universitas Airlangga Surabaya dan Laboratorium Fisiologi Fakultas Kedokteran Universitas Brawijaya Malang. Sampel menggunakan mencit (Mus musculus) model endometriosis. Penelitian dibagi menjadi 8 kelompok, yaitu : kontrol negatif, kontrol positif dan perlakuan pemberian genistein dosis 50 mg/hari, 100 mg/hari, 200 mg/hari, 300 mg/hari, 400 mg/hari, 500 mg/hari. Pengukuran kadar MMP-2 dan MMP-9 menggunakan ELISA kit. Data hasil pengamatan dianalisis dengan uji ANOVA, Tukey dan Regresi.Hasil: Genistein mampu menurunkan kadar MMP-2 terendah pada dosis 50 mg/hari (5.61±0.69 ng/mL), 100 mg/hari (4.91±0.59 ng/mL) dan 400 mg/hari (4.95±0.32 ng/mL) bila dibandingkan dengan perlakuan dosis yang lain.  Genistein mampu menurunkan kadar MMP-9 terendah pada dosis 50 mg/hari (1.69±0.45 ng/mL), 100 mg/hari (1.29±0.32 ng/mL), 200 mg/hari (1.84±0.25 ng/mL) dan 400 mg/hari (1.42±0.11 ng/mL) bila dibaningkan dengan perlakuan dosis yang lain.Kesimpulan: Genistein dapat menurunkan kadar MMP-2 dan MMP-9 dalam cairan peritoneal mencit (Mus musculus) model endometriosis.Saran: Perlu studi lanjut untuk melihat pengaruh genistein pada hewan coba kelinci Kata Kunci: Endometriosis, Genistein, MMP-2, MMP-9 ABSTRACT Background: Matrix metalloproteinases (MMPs) affect the progression of endometriosis, in the invasion , development , and metastasis of endometriosis. Reducing levels of MMP-2 and MMP-9 is an appropriate therapy for endometriosis cases using natural hormonal therapy, namely genistein.Purpose: To know the effect of genistein on the level of MMP-2 and MMP-9 in peritoneal fluid of endometriosis mice model.Method: The design of this study was experimental, had been performed in Reproductive Physiology and Embryology Laboratory, Veterinary Medical Faculty of Airlangga University Surabaya and Physiology Laboratory, Medical Faculty of Brawijaya University Malang. The animal model was divided the 8 groups: negative group ,positive group, and treated group with genistein doses of 50 mg/day, 100 mg/day, 200 mg/day, 300 mg/day, 400 mg/day, and 500 mg /day (human dose). The measurement of MMP-2 and MMP-9 levels using ELISA kits. Thedata were analyzed with ANOVA, Tukey, and Regression tests.Result: Genistein was able to decrease the MMP-2 level in the lowest dose 50 mg/day (5.61±0.69 ng/mL), 100 mg/day (4.91±0.59 ng/mL) and 400 mg/day (4.95±0.32 ng/mL) when compared with other treatment dose. Genistein was able to decrease the MMP-9 level in the lowest dose 50 mg/day (1.69±0.45 ng/mL), 100 mg/day (1.29±0.32 ng/mL), 200 mg/day (1.84±0.25 ng/ mL) and 400 mg/day (1.42±0.11 ng/mL) when compared with other treatment doses.Conclusion: Genistein may decrease MMP-2 and MMP-9 levels in peritoneal fluid of endometriosis mice model.Suggestion: Further study is needed to see the effect of genistein on rabbit experimental animals. Keywords: Endometriosis, Genistein, MMP-2, MMP-9 
Pengaruh Faktor Internal Keluarga dan Peran Petugas Kesehatan terhadap Pemberian Asi Eksklusif Evalina Fajriani; Eko Sulistijono; Endang Sri Wahyuni
Majalah Kedokteran Indonesia Vol 68 No 8 (2018): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.68.8-2018-47

Abstract

Introduction: Great global attention isbeing given regarding the realization of exclusive breastfeeding, including by the Indonesian government. The realization of this in Indonesia has not achieved the desired percentage, including in Lombok Utara Regency, which is still approximately 61.2%. Other regions in the Province of NTB, one of which is Lombok Barat Regency, has been able to realize an exclusive breastfeeding percentage of 96.42%. Research on factors that affect exclusive breastfeeding in those two regions becomes very important to be done. Objectif: to find out the significance of the effect of internal family factors (family structure, mother’s education, father’s education, mother’s occupation, father’s occupation, mother’s knowledge, father’s knowledge, and history of antenatal care) and to find out the role of health officials in the promotion of exclusive breastfeeding at the region of the Kuripan Community Health Center, Lombok Barat Regency, and Gangga Community Health Center, Lombok Utara Regency. Method: The research design is a quantitative associative research; data collection was done by distributing questionnaires and data analysis used logistic regression. Results: Out of 170 respondents for the research, it was found that 54.1% did not exclusively breastfeed and the other 45.9% did. Family factorswere found to have an evaluation of moderate criteria in providing support (average score of 3.34): the level of the mother’s education was in general still low (37.65% only completed and did not complete elementary school), where the criteriais the same relative to the father’s education and mother’s occupation (the latter generally non-employees, by 81.18% and 80.59% respectively),while mother’s and father’s knowledge on exclusive breastfeeding was still at moderate criteria, the mothers generally partake in antenatalcare (78,8%), andthe role of health officials is still not yet optimal (average score of 3.25). The resulting logistic regression model fulfilled the fit criteria of the model, which means that each added variable in the model will increase the fit of the equation. The Wald test (partial significance) found that only mother’s knowledge and the role of health officials have a significant effect on the alpha 5.0% on the probability of mothers exclusively breastfeeding in the regions of the Kuripan and Gangga Community Health Centers. Conclusion: It can be stated that the vital role in realizing exclusive breastfeeding is that of the officials, who directly guided to increase maternal knowledge, awareness, and formation of behaviors