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KARAKTERISASI PROTEASE Bacillus subtilis A1 InaCC B398 YANG DIISOLASI DARI TERASI SAMARINDA Yati Sudaryati Soeka; Sulistiani Sulistiani
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.694

Proteases is enzyme that breaks the peptide bond to produce amino acids and simpler peptides. This enzyme can be isolated from a variety of sources such as plants, animals and microbe. Alkaline proteases of microbial origin possess considerable industrial potential due to their biochemical diversity and wide applications in tannery, food, medicinal formulations and detergents. The objectives of the research was to determine the characteristics of the protease enzyme produced by strain A1, including incubation time, substrate concentration azokasein, the optimum temperature and pH also stability. The effect of some metal ions as activators or inhibitors of the protease enzyme activity measured with a spectrophotometer at ? 280 nm. Strain A1 was identified by using 16S rDNA sequencing and phylogenetic analysis based on Neighbor Joining method. Strain A1 protease activity was qualitatively demonstrated the presence of a clear zone around the colonies in the medium containing 1% skim milk. Result showed that the highest activity were incubation time of three days, temperature of 50 ºC and pH 8.5 were 87.35 U/mL, 83.44 U/mL and 93.11 U/mL, respectively. Effect of metal ions in the form of divalent and monovalent cations at a concentration of 1 mM on protease A1 activated by divalent cations CaCl2, MnCl2 while divalent cations CuCl2, HgCl2 and monovalent cations KCl, NaCl were inhibitors of each enzyme activity. Result from molecular identification based on 16S rDNA sequence and phylogenetic analysis using Neighbor Joining method suggested that strain A1 was Bacillus subtilis. The strain was registered in the InaCC collection (no. B 398).

PELAKSANAAN PERJANJIAN KERJA ANTARA KARYAWAN DENGAN PT. BAHARI SANDI PRATAMA PEKANBARU DIKAITKAN KINERJA KARYAWAN Sulistiani Sulistiani; Zulfikar Jayakusuma; Dasrol Dasrol
Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum Vol 6, No 2 (2019): Juli - Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum

Show Abstract | Download Original | Original Source | Check in Google Scholar

According to Law Number 13 of 2003 concerning Manpower contains the rights and obligations of workers. One of the obligations of workers contained in this Manpower Act, namely in Article 102 paragraph 2, the performance of workers is the obligation of workers after the company gives its rights to workers, PT. Bahari Sandi Pratama Pekanbaru is a sea transportation shipping company that has a target every year. The purpose of this study is to determine the implementation of work agreements between employees and PT. Bahari Sandi Pratama Pekanbaru is associated with employee performance. This type of research is classified in the type of sociological legal research, namely research on the effectiveness of existing law, the nature of this research is descriptive research that describes systematically, the facts and characteristics of the object under study appropriately. Research conducted at PT. Bahari Sandi Pratama Pekanbaru, while the population and sample are all parties involved in this study, both the chief financial officer and employees. Sources of data used are primary and secondary data, data collection techniques used by interviews, questionnaires and literature review. From the results of the research problem there are two main things that can be concluded, First, the implementation of the work agreement between the employee and PT. Bahari Sandi Pratama Pekanbaru was not carried out properly. Employees have defaulted by not carrying out what was promised. Secondly, internal factors, namely employees themselves, excessive workloads which lead to concurrent positions and multiple duties and the lack of strict application of sanctions from the company itself, listen to external factors namely a less comfortable work environment that does not increase employee performance. The author's suggestion, First, should the parties in the work agreement must fulfill what is their rights and obligations as agreed between the employee and PT. Bahari Sandi Pratama Pekanbaru. Second, to minimize the causes of employees not improving performance at PT. Bahari Sandi Pratama Pekanbaru should the parties be able to pursue all rights and obligations properly for the good of both parties Keywords: work agreements, performance, employees, employer.

PELAKSANAAN PERJANJIAN KERJA ANTARA KARYAWAN DENGAN PT. BAHARI SANDI PRATAMA PEKANBARU DIKAITKAN KINERJA KARYAWAN Sulistiani Sulistiani; Zulfikar Jayakusuma; Dasrol Dasrol
Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum Vol 6, No 2 (2019): Juli - Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum

Show Abstract | Download Original | Original Source | Check in Google Scholar

According to Law Number 13 of 2003 concerning Manpower contains the rights and obligations of workers. One of the obligations of workers contained in this Manpower Act, namely in Article 102 paragraph 2, the performance of workers is the obligation of workers after the company gives its rights to workers, PT. Bahari Sandi Pratama Pekanbaru is a sea transportation shipping company that has a target every year. The purpose of this study is to determine the implementation of work agreements between employees and PT. Bahari Sandi Pratama Pekanbaru is associated with employee performance. This type of research is classified in the type of sociological legal research, namely research on the effectiveness of existing law, the nature of this research is descriptive research that describes systematically, the facts and characteristics of the object under study appropriately. Research conducted at PT. Bahari Sandi Pratama Pekanbaru, while the population and sample are all parties involved in this study, both the chief financial officer and employees. Sources of data used are primary and secondary data, data collection techniques used by interviews, questionnaires and literature review. From the results of the research problem there are two main things that can be concluded, First, the implementation of the work agreement between the employee and PT. Bahari Sandi Pratama Pekanbaru was not carried out properly. Employees have defaulted by not carrying out what was promised. Secondly, internal factors, namely employees themselves, excessive workloads which lead to concurrent positions and multiple duties and the lack of strict application of sanctions from the company itself, listen to external factors namely a less comfortable work environment that does not increase employee performance. The author's suggestion, First, should the parties in the work agreement must fulfill what is their rights and obligations as agreed between the employee and PT. Bahari Sandi Pratama Pekanbaru. Second, to minimize the causes of employees not improving performance at PT. Bahari Sandi Pratama Pekanbaru should the parties be able to pursue all rights and obligations properly for the good of both parties Keywords: work agreements, performance, employees, employer.

Characterization of Protease Crude Extract from Indigenous Lactic Acid Bacteria and the Protein Degradation Capacity in Local Tuber and Cereal Paste Flour Tatik Khusniati; Nanda Sabbaha Nur Kasfillah; Vilya Syafriana; Resti Sofia Zahara; Padmono Citroreksoko; Sulistiani Sulistiani; Trisanti Anindyawati
Jurnal Kimia Terapan Indonesia Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Protease hidrolyzed protein in flour in order to more digest by human ulcer. Lactobacillus plantarum B110 and Lactobacillus satsumensis are indigenous lactic acid bacteria that produce protease. The objective of this research is to characterization of protease crude extract from indigenous lactic acid bacteria and the protein degradation capacity in local tuber and cereal paste flour. Tuber and cereal flour used were purple sweet potato (Dioscorea alata), cassava (Manihot esculenta), rice (Oryza sativa), corn (Zea mays) and wheat (Triticum) as comparison. Proteaseactivity was tested by Horikoshi method (1971) and protein degradation was by formol titration. Research results showed that optimum activities and stabilities of Lactobacillus plantarum B110 were at pH: 7.5, 45oC and pH:5.0-8.0, 35-50oC, while that L. satsumensis EN 38-32 were at pH: 7.0, 40oC and pH:6.0-8.0, 20-45oC. Increases in protein degradation capacity of the paste flour additional proteases crude extract from L. plantarum B110 were 0.0838% (purple sweat potato), 1.3299% (cassava), 0.5834% (corn), 0.7499% (rice) and 1.5551% (wheat as comparison); while that L. satsumensis EN 38-32 were 0.20% (purple sweet potato), 0.32% (cassava), 0.87% (corn), 1.17% (rice). Based on increases in protein degradation capacity, protease crude extract from L. plantarum B110Â and L. satsumensis EN 38- 32 were sequently better to hidrolyze protein of cassava and rice paste flour than thatother tuber and cereal.

PURIFIKASI PARSIAL DAN KARAKTERISASI ÃŸ-GALAKTOSIDASE Lactobacillus plantarum B123 INDIGENOS DAN HIDROLISIS LAKTOSA UNTUK PRODUKSI SUSU ULTRA HIGH TEMPERATURE RENDAH LAKTOSA Tatik Khusniati; Neny Mariyani; Hanifah Nuryani Lioe; Didah Nur Faridah; Abdul Choliq; Sulistiani Sulistiani
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Î²-Galactosidase is enzyme which hidrolyze lactose to glucose and galactose. This enzyme is used in production low lactose milk for consumption human which have lactose intolerance. Partial purification of Î²-galactosidase is important to be conducted to increaseÂ Î²-galactosidase activity in order to its hydrolysis potency on UHT milk lactose increased.This research was aimed to production by partially purification and characterization indigenous Î²-galactosidase from Lactobacillus plantarum B123, and lactose hydrolysis for production low lactose UHT milk. Partially purification were precipitation following dialysis. Characterization included optimazion and stabilization of enzyme, while lactose hydrolisis for production low lactose UHT milk was detected by enzymatic GOD-POD kit. The results showed that production of Î²-galactosidase by using partial purification increased from 21.51 Â± 0.23 U/mL (crude) to 106.34 Â± 0.56 U/mL (dialysis).Â The optimum crude Î²-galactosidase activity was reached in precipitation by using 60 % ammonium sulphate.Â The purity of crude Î²-galactosidase increased 3.71 times after precipitation, and 14.28Â timesÂ after dialysis. Characterization of Î²-galactosidase showed thatÂ optimum activities of crude and dialyzed Î²-galactosidase were at pH 6.5 and 50 oC, respectively. Stability of crude Î²-galactosidase incubated for 1 h were at pH: 5.0-8.5 and 25-50 Â°C. Specific activity of crude Î²-galactosidase was 15.05 U/mg protein, while that dialyzed Î²-galactosidase was 109.58 U/mg protein. Lactose hidrolysis to produce low lactose UHT milk showed that glucose concentration increased with the increase of hidrolysis time. Time needed to hidrolyze lactose 50 % with 4.8 U/mL Î²-galactosidase at 50Â°C was 6.08 h. In conclusion that indigenous Î²-galactosidase from Lactobacillus plantarum B123 purified partially can be used as lactose hidrolyzer in production of low lactose UHT milk.Key words : b-galactosidase, indigenous Lactobacillus plantarum B123, purification, lactose hidrolysis, UHT Milk

Penggunaan Gadget Terhadap Perkembangan Sosial Anak Usia Dini di Raudhatul Athfal (RA) Fastabiqul Khairaat Kelurahan Airmadidi Atas Kecamatan Airmadidi Ismail K Usman; Sulfa Potiua; Sulistiani Sulistiani
Indonesian Journal of Early Childhood Education (IJECE) Vol 2 No 01 (2022): IJECE Volume 2 Nomor 1, Juni 2022
Publisher : Program Studi Pendidikan Islam Anak Usia Dini (PIAUD) Fakultas Tarbiyah dan Ilmu Keguruan IAIN Manado

Penelitian ini bertujuan untuk mengetahui bagaimana penggunaan dan dampak gadget di RA Fastabiqul Khairaat Kelurahan Airmadidi Atas Kecamatan Airmadidi dengan melihat kaitan antara perkembangan sosial emosional anak dan penggunaan gadget seperti bermain game, Youtube, dan lainnya. Penelitian ini merupakan penelitian kualitatif dengan menggunakan teknik observasi, wawancara, dan dokumentasi. Data yang diperoleh dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukan bahwa perkembangan sosial anak usia dini dalam penggunaan gadget masih ada yang belum berkembang dengan baik dan ada juga yang sudah sesuai harapan. Hal tersebut tak lepas dari pola asuh dan pengawasan oleh orang tua, karena sebagian besar orang tua terkesan memberikan gadget dan tidak terlalu khawatir dengan dampak yang akan ditimbulkan dari penggunaan gadget secara terus-menerus. Orang tua harus lebih berhati –hati dalam mengawasi dan memonitoring kegiatan anak dalam menggunakan gadget sehari-hari untuk meminimalisir sisi negatif yang ditimbulkan dari penggunaan gadget tersebut, dan seharusnya gadget digunakan dan dimanfaatkan untuk hal yang positif Kata kunci: Gadget, Perkembangan Sosial, Anak Usia Dini

Improving Learning Achievement Second Grade of Theme 5 Subtheme 3 Lesson 3 by Problem Based Learning Model Sulistiani Sulistiani
Social, Humanities, and Educational Studies (SHES): Conference Series Vol 4 (2021): Social, Humanities, and Educational Studies (SHEs): Conference Series (Special Issue 1
Publisher : Universitas Sebelas Maret

Berdasarkan observasi awal di SD Negeri 6 Suwawal terutama pada kelas 2 ditemukan masalah bagaimana cara membuat kalimat yang sopan dalam meminta maaf kepada orang lain karena melakukan suatu kesalahan, mengukur barang dan menjumlahkannya. Permasalahan dari kehidupan sehari-hari (soal cerita) yang membuat para peserta didik bingung untuk menentukan itu ditambah atau dikurangi. Mereka juga masih belum bisa menyanyi sambil menirukan sebuah gerakan alam. Tujuan dari penelitian ini adalah untuk mengetahui hasil belajar melalui penerapan model problem based learning (PBL) di SD Negeri 6 Suwawal pada tahun pelajaran 2020/2021. Subjek penelitian ini adalah siswa kelas 2 SD Negeri 6 Suwawal. Jumlah peserta didiknya adalah 20 peserta didik. Peserta didik terdiri dari 13 peserta didik laki- laki dan 7 peserta didik perempuan. Sebelum dilakukan penelitian (pra siklus) ketuntasan hasil belajarnya adalah 35% dan setelah penelitian siklus I menjadi 55% dan pada siklus II menjadi 85%. Hasil penelitian menunjukkan bahwa adanya peningkatan hasil belajar peserta didik. Dari penelitian ini dapat disimpulkan bahwa penerapan model problem based learning (PBL) dapat meningkatkan hasil belajar pada tema 5 subtema 3 pembelajaran 3.

Factors Affecting The Risk of Postpartum Depression Sulistiani Sulistiani; Sri Handayani; Cesa Septiana Pratiwi
Jurnal Health Sains Vol. 3 No. 10 (2022): Jurnal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v3i10.851

Postpartum depression is a common problem that occurs in the perinatal period around 7-13% of postpartum women can cause short-term and long-term effects that will have an impact on the mother, baby, and social environment. This study aimed to examine the evidence related to the factors that influence the risk of postpartum depression. Scoping review using the framework of Arksey and O'Malley, who conducted a focused review with the PEO framework, conducted a literature search using relevant databases and gray literature, selected relevant studies using inclusion and exclusion criteria, and conducted a critical assessment for assessment. article quality, perform data extraction, analyze and report results. 10 articles were selected and received Grade A. 10 articles were obtained from research conducted in developing countries, namely 2 articles from Nepal, 1 from Malaysia, 1 from Uganda, 2 articles from Indonesia, 1 from Egypt, 1 from Kosovo, 1 Iran, and 1 an article from Ethiopia 14 factors that influence the risk of postpartum depression, namely age, parity, gender, unplanned/expected pregnancy, family mental health history, social support, history of anxiety/depression, family problems, obstetric complications, parenting stress, type of delivery, financial problems, spouse violence. Screening and intervention programs for women prone to postpartum depression should be carried out during pregnancy and are recommended to address perinatal mental health problems and improve maternal mental health.

PERAN BIDAN PADA PENATALAKSANAAN TOKOFOBIA PADA WANITA HAMIL Sulistiani Sulistiani; Cesa Septiana Pratiwi
Jurnal Kesehatan - STIKes Prima Nusantara Vol 13 No 2 (2022): Jurnal Kesehatan
Publisher : LPPM Universitas Prima Nusantara Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35730/jk.v13i2.738

Latar Belakang: Tokofobia (Fobia melahirkan) adalah ketakutan seseorang dalam menghadapi proses persalinan yang dipengaruhi factor psikologis dan fisiologis. Tokophobia sering ditandai dengan ketakutan yang berlebihan dan disertai dengan menghindari untuk hamil bahkan memiliki keinginan untuk aborsi, dan menghindari untuk bersalin pervaginam yang mengakibatkan permintaan Caesar karena tokofobia sering terjadi. Tokofobia bisa terjadi karena memiliki takut akan nyeri persalinan, kekhawatiran terhadap bayinya, ataupun memiliki pengalaman traumatis atas persalinan sebelumnya.Tujuan Review: Untuk mengetahui peran bidan pada penatalaksanaan tokofobia (Fobia melahirkan) pada Wanita hamil.Metode: Metode Scoping review format: Population, the Phenomena of Interest and the Context (PICO).Kesimpulan: 6 artikel dilakukan review didapat hasil seleksi 5 dari negara maju dan 1 dari negara berkembang, terdapat 2 artikel dari Swedia, 1 artikel dari Norwegia, 1 dari Australia, 1 dari Finlandia dan 1 dari Iran. Tenaga Kesehatan terutama bidan memiliki peran penting dalam mengurangi rasa takut yang berlebihan salah satu cara dengan memberikan konseling dapat mengubah sudut pandang Wanita akan ketakutan yeng mereka alami dengan memberikan dukungan psikologis maupun fisiologis. Dukungan yang dilakukan oleh bidan dapat mengurangi angka permintaan Sectio Caesaria.

Hadis dan Ushul Fiqh: Studi Tentang Peran Hadis dalam Menentukan Hukum Islam Ola Alifiyanti Zahra Purnama; Sulistiani Sulistiani; Mamay Humaeroh; Hafidz Taqiyuddin
Hikmah : Jurnal Studi Pendidikan Agama Islam Vol. 2 No. 2 (2025): Juni: Hikmah : Jurnal Studi Pendidikan Agama Islam
Publisher : Asosiasi Riset Ilmu Pendidikan Agama dan Filsafat Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/hikmah.v2i2.878

In this study discusses the role of Hadith as the second source of Islamic law after the Qur'an, which serves as a guide to life in explaining and detailing Islamic laws. The author uses a qualitative approach to literature studies, collecting information from various books and relevant journals. The discussion covers the understanding of Hadith, its types, and its relationship with the Qur'an, including the challenges in understanding Hadith in the modern era. The results of this study show that Hadith is important in explaining the verses of the Qur'an that are general in nature and become the basis in the process of ijtihad. Conclusion in this study a deep understanding of the Hadith is very important for Muslims to run the teachings according to the guidance of the Prophet Muhammad.

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