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Seduhan Bubuk Kopi Robusta Spray Dryed Meningkatkan Viabilitas Monosit yang Dipapar Eksotoksin Streptococcus mutans (In Vitro) Fiftiani Syarah; I Dewa Ayu Susilawati; Nadie Fatimatuzzahro
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 16 No 1 (2019)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v16i1.19956

Abstract

Monocytes response against bacterial exotoxins can cause cell damage, due to the production of antimicrobial compounds such as oxidants and free radicals. Spray dryed robusta coffee brewed contained antioxidants that was alleged to be able counteract oxidants and free radicals leading to prevent cell damage. This research aimed to prove the effect of spray dryed robusta coffee brew (SDRCB) to increase monocytes viability after being exposed by exotoxins of Streptococcus mutans (in vitro). This in vitro experimental study used the post test only control group design. The object of study was human peripheral blood monocytes, isolated by means of gradient centrifugation method. Monocyte viability was analyzed by trypan blue assay. The parameter was the percentage viable monocytes. Data were analyzed using Anova and LSD. Monocytes incubated with SDRCB showed a significant (p<05) higher viability (96.95%) than monocytes without SDRCB (94,27%). Spray dryed robusta coffee brew proved to be able increase viability of monocytes after S.mutans exposure (in vitro).
Kajian Pustaka: Sumber Reactive Oxygen Species (ROS) Vaskular I Dewa Ayu Susilawati
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 18 No 1 (2021)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v18i1.27959

Abstract

Increasing amount of vascular reactive oxygen species (ROS) play a key role in the pathogenesis of almost all stages of cardiovascular diseases (CVD), such as atherosclerosis, hypertension, myocardial infartion, etc. This paper aimed to present a brief review of the important vascular sources of ROS. Vascular ROS is mainly produced by enzymatic sources in the vascular cells, i.e. phagocytic inflammatory cells (neutrophils, monocytes/macrophages), and nonphagocytic cells (endothelium, smooth muscle cell and fibroblast). Enzymatic sources of vascular ROS are NADPH oxidase, xanthine oxidase, eNOS, lipoxygenase, cyclooxygenase, and mitochondrial electron transport. In conclusion, over production of ROS induce oxidative burst and will be detrimental for cardiovascular health. A better understanding of ROS would be worwhile for developing method for preventing CVD based on ROS inhibition.
Mekanisme Hipotetikal Efek Konsumsi Ikan Menurunkan Resiko Serangan Jantung I Dewa Ayu Susilawati
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 15 No 1 (2018)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v15i1.17905

Abstract

Potential maritime Indonesia related health science has not been widely explored. Studies in Indonesia about the potential of marine resources to improve health are still very little. This article summarized a reports from magazines and journals in foreign countries about the potential fish consumption lowers the risk of heart attack. Allegedly this potential is related to the content of unsaturated fatty acids omega-3 (n-3) on fish. The report of observations, clinical , animal and in vitro studies have demonstrated the benefits of omega-3 prevent and treat cardiovascular disease. Although the mechanism is not clear, but it has been shown that the cardioprotection effect of n-3 fatty acids may be caused by its role influencing biological effects on lipid and lipoprotein metabolism, platelet function, arterial cholesterol distribution, vascular function and inflammatory response
Periodontal infection is a “silent killer”* I Dewa Ayu Susilawati
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 8 No 1 (2011)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Periodontal infection contained at many pro-oxidatif toxity components which is easily spreading through flood circulation syslimicly, and potentially acts as antigent and induces inflamating response inflamation processes is a front line defence respons to infection. However it is very dangerous if inflamation is oceure is a vital asam such as in brain and heart, could be of a life threatering. The spreading of Periodontal infection through Blodd steam is usually asymoptpmatically. Thas patients are unaware that their left is in a thred Periodontal infection is a “silent killer”.
Antibacterial activity of robusta and arabica coffee husks extracts against Lactobacillus acidophilus: Experimental study Ishimora, Marina Erlysa; Prasetya, Rendra Chriestedy; Susilawati, I Dewa Ayu
Padjadjaran Journal of Dental Researchers and Students Vol 7, No 3 (2023): October 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v7i3.48658

Abstract

Efek Ekstrak Ampas Seduhan Bubuk Kopi Dalam Menghambat Produksi Malondialdehid Pada Isolat Monosit Perifer Manusia yang Distimuli Dengan Eksotoksin Streptococcus mutans Fawwazi, Rafif Hanif; Harmono, Happy; Susilawati, I Dewa Ayu
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 21 No 1 (2024)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/stoma.v21i1.47332

Abstract

Monocytes as non-specific immune cells provide an early response to pathogens and foreign objects that enter the body. When exposed to pathogens or foreign objects, monocytes experience an increase in the number of free radicals. Free radicals activate phospholipase enzymes that break down membrane phospholipids into phosphate, glycerol and fatty acids. Unsaturated fatty acids (PUFAs) react with free radicals to undergo peroxidation to produce malondialdehyde (MDA). It takes antioxidant compounds that can reduce excess free radicals in the body. Coffee grounds as solid waste from the consumption of processed coffee products are not used and disposed of, it is suspected that they can be used as antioxidants. This study aims to determine the effect of coffee grounds steeping antioxidants on the profile of MDA levels in monocytes stimulated by the S. mutans exotoxin (In Vitro). Research was done in experimental post test only control group design. The object of this study was human peripheral blood monocytes, which were isolated by the Gradient Density method. Following in vitro exposure to the exotoxin S. mutans, the monocyte isolates were subjected to treatment with brewed coffee grounds extract at three different concentrations (0.25 mg/ml, 0.50 mg/ml, and 0.75 mg/ml). The MDA levels were subsequently determined by employing the TBArs method on a UV-Vis spectrophotometer. The results of MDA levels were analyzed using One Way Anova and LSD. The MDA level data showed significance (p<0.05) with the average MDA level in the Negative Control group1.49 g/ml; Positive Control 10.62 g/ml; T1 8.15 g/ml; T2 6.59 g/ml; and T3 4.69 g/ml. It can be concluded that the extract of brewed coffee grounds can reducing MDA levels in monocytes exposed to the exotoxin of S. mutans.
Antibacterial Activity of Robusta Instant Coffee Powder Against Acidogenic and Aciduric Species of Streptococcus mutans Bacteria Lestari, Pujiana Endah; Susilawati, I Dewa Ayu; Harmono, Happy; Dewanti, I Dewa Ayu Ratna; Masari, Al; Nailufar, Khanun; Morauli, Fatricia
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.50812

Abstract

Streptococcus mutans is a major species that causes dental caries due to its acidogenic and aciduric properties. Coffee beans contain several compounds, such as caffeine, phenols, tannins, trigonelline, chlorogenic acid, and caffeic acid, which are known for their antibacterial properties. Robusta instant coffee powder is a stable product that is not easily contaminated with bacteria, fungi, and yeast. It is a zero-waste and soluble material. This study aimed to analyze the antibacterial activity of robusta instant coffee powder (RICP) against S. mutans. The antibacterial activity of RICP against S. mutans was tested using three antimicrobial susceptibility tests: 1) disk diffusion method, 2) minimum inhibitory concentrations (MIC), and 3) minimum bactericidal concentration (MBC). The results of the disk diffusion method test showed that the RICP concentration of 0.2 g/mL to 0.8 g/mL against S. mutans had an inhibition zone of 31.14 mm to 42.01 mm. MIC and MBC values of RICP against S. mutans were both 0.0313 g/mL. In conclusion, robusta instant coffee powder (RICP) demonstrates antibacterial activity against the acidogenic and aciduric S. mutans bacteria.