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Profil Hormon dan Kinerja Reproduksi Ikan Sidat (Anguilla bicolor McClelland) yang Tertangkap di Perairan Segara Anakan Cilacap Nur Rachmawati, Farida; Susilo, Untung
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Ikan Sidat, Anguilla bicolor McClelland, pada kondisi alami atau budidaya jarang ditemukan pada fase matang gonad, sehingga upaya untuk melakukan pembenihan buatan sulit dilakukan. Oleh karena itu, diperlukan informasi yang berkaitan dengan aspek reproduksinya, karena masih terbatasnya informasi tersebut. Penelitian ini bertujuan mengetahui profil hormon dan kinerja reproduksi Ikan Sidat. Penelitian ini dilakukan dengan metode survey. Ikan Sidat diperoleh dari perairan segara Anakan Cilacap. Bobot gonad ikan ditimbang untuk mengamati tingkat kematangan gonad (GSI), sedangkan serum hormon steroid diukur menggunakan metode ELISA. Hasil penelitian menunjukkan bahwa nilai GSI (Gonado Somatic Index) berkisar antara 0,1â€“2,88%, sedangkan kadar hormon estradiol berkisar antara 9â€“458,94 (pg/ml); Progesteron 0,25â€“0,57 (ng/ml) dan Testosteron < 0,1 (ng/ml). Dengan demikian dapat disimpulkan bahwa perkembangan gonad ikan Sidat mengalami peningkatan seiring dengan peningkatan serum hormon steroid, khususnya estradiol dan progesteron.

Profil Hormon dan Kinerja Reproduksi Ikan Sidat (Anguilla bicolor McClelland) yang Tertangkap di Perairan Segara Anakan Cilacap Farida Nur Rachmawati; Untung Susilo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.103

Ikan Sidat, Anguilla bicolor McClelland, pada kondisi alami atau budidaya jarang ditemukan pada fase matang gonad, sehingga upaya untuk melakukan pembenihan buatan sulit dilakukan. Oleh karena itu, diperlukan informasi yang berkaitan dengan aspek reproduksinya, karena masih terbatasnya informasi tersebut. Penelitian ini bertujuan mengetahui profil hormon dan kinerja reproduksi Ikan Sidat. Penelitian ini dilakukan dengan metode survey. Ikan Sidat diperoleh dari perairan segara Anakan Cilacap. Bobot gonad ikan ditimbang untuk mengamati tingkat kematangan gonad (GSI), sedangkan serum hormon steroid diukur menggunakan metode ELISA. Hasil penelitian menunjukkan bahwa nilai GSI (Gonado Somatic Index) berkisar antara 0,1â€“2,88%, sedangkan kadar hormon estradiol berkisar antara 9â€“458,94 (pg/ml); Progesteron 0,25â€“0,57 (ng/ml) dan Testosteron < 0,1 (ng/ml). Dengan demikian dapat disimpulkan bahwa perkembangan gonad ikan Sidat mengalami peningkatan seiring dengan peningkatan serum hormon steroid, khususnya estradiol dan progesteron.

Efektivitas Zat Aktif Fipronil pada Berbagai Substrat Fagostimulan untuk Pengendalian Kecoak Jerman (Blattella germanica L.) Riska Isna Maula; Hery Pratiknyo; Untung Susilo; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

German cockroach (Blattella germanica L.), is a residential insect pest and vector of various diseases. To control its population, alternative chemical control models with Fipronil 0.03% are now being developed. Fipronil has been proven to be effective and has no resistance. To increase the effectiveness of the bait in controlling German cockroaches, a fipronil 0.03% combination needs to be developed with a phagostimulant bait of bananas, durian, erythisol sugar, and sugar formulation, so this study needs to be done. This study used an experimental method with a randomized block design (RBD), five treatments such as A: bait with a combination of banana phagostimulant and 0.03% fipronil, B: bait with a combination of durian phagostimulant and 0.03% fipronil, C: bait with a combination erythisol phagostimulant and 0.03% fipronil, D: bait with a combination of sugar phagostimulant and 0.03% fipronil, K: bait with a combination of matrix and fipronil 0.03% (control positive) was used for the interest test, and bait with a matrix without fipronil 0.03% (control negative) was used for mortality testing. The observations the average results for durian, banana, sugar formulated, erytrisol sugar and controls, respectively 20%, 20%, 21.6%, 12.4%, and 22.8%, while the results bait consumed with durian stimulation are 0.29g, banana 0.31g, sugar formulation 0.28g, erytrisol sugar 0.26g and control 0.24g. However, based on statistical tests, the results of the interest test with the feed consumption test between treatments showed no significant difference (p>0.05). The test results in mortality of cockroaches Germany's feed with phagostimulan durian, banana, formulations sugar, sugar erytrisol and control consecutively 98% 100 %, 98%, 98%, and 20%, based on the statistical test there is a significant difference (p<0.05), where the difference based on the Duncan test is their treatment compared to control.

Peran Hormon Kortisol dalam Osmoregulasi Ikan Sidat, Anguilla bicolor, pada Lingkungan Bersalinitas Untung Susilo; Farida Nur Rachmawati; Sorta Basar Ida Simanjuntak
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.281

The osmoregulatory capacity of Anguilla bicolor in the sea water is influenced by hormonal activities. Therefore, the aim of this study was to know the influence of cortisol on osmoregulation of the eel at the different levels of salinity medium. An experimental method with six treatments on randomized completely design was used in this study. The treatments were (1) fish acclimated at water salinity 15 ppt without hormone injection; (2) fish acclimated at water salinity of 30 ppt without hormone injection; (3) fish acclimated at water salinity of 15 ppt and injected with 4 µg cortisol/gr body weight; (4) fish acclimated at water salinity 15 ppt and injected with 8 µg cortisol/gr body weight; (5) fish aclimated at water salinity of 30 ppt and injected with 4 µg cortisol/g body weight; (6) fish acclimated at water salinity of 30 ppt and injected with 8 µg cortisol/g body weight. All of the treatments were replicated four times. Data were analyzed using One way ANOVA followed by Least Significant Difference. The results showed that the cortisol has significant effect (P<.05) on plasma osmolality only at the early of acclimation on medium 30 ppt after injection, especially at 6 and 12 hours after cortisol treatment, and there was no significant efffect of cortisol treatment (P>.05) if the acclimation increased. Osmoregulatory capacity on medium of 15 ppt and 30 ppt were influenced by cortisol treatment at 6 hours acclimation (P<.05), but only that aclimated in medium opf 5 ppt was influenced by cortisol treatment at 12 hours acclimation. Increased acclimation on medium of 15 ppt and 30 ppt was not influenced by cortisol treatment (P>.05). Water body content was not influenced by cortisol treatment (P>.05) at all medium acclimation. At six hour acclimation, treatment cortisol has significant difference (P<.05) on hematocrite only at medium 30 ppt, but cortisol treatment has significant difference (P<.05) at medium 15 ppt and 30 ppt in 12 hours acclimation. Increased acclimation has no significant difference (P>.05) on treatment cortisol. Cortisol injection has no significant difference (P>.05) on plasma glucose on all medium and only on 7 days aclimation, the plasma glucose has significant difference (P<.05) after injected by cortisol. It could be concluded that cortisol treatment has a role on eel osmoregulation at early acclimation. Haematocrite account was also influenced by cortisol injection, but only at the early acclimation. Water body content, blood glucose and total body energy were not influenced by cortisol.

Aktivitas Protease dan Komposisi Proksimat Tubuh Ikan Sidat (Anguilla bicolor Mcclelland) pada Kondisi Puasa dan Pemberian Pakan Kembali Untung Susilo; Nuning Setyaningrum; Farida Nur Rachmawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.133

Protease activity and body proximate composition of eel, Anguilla bicolor McClelland., under starvation and refeeding conditions with three treatments and four replications in randomized completely design has been evaluated. The result showed that the protease activity of eel was no significant different (P>.05) between treatment, but body proximate composition has significant differences (P<.05), especially in protein and lipid contents at starvation condition. It can be concluded that the eel protease activity did not change under starvation and refeeding condition, but the lipid content decreased at starvation condition.

Aktivitas Protease dan Kadar Protein Tubuh Ikan Lele Dumbo (Clarias gariepinus) pada Kondisi Puasa dan Pemberian Pakan Kembali Wieka Mahalida Hanum; Untung Susilo; Slamet Priyanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.121

Three treatments and four replications in randomised completely design were conducted to reveal the protease activity and body protein content on catfish, Clarias gareipinus, under starvation and refeeding conditions. The result showed that the protease activity has no significant different (P>.05) in first week, but has significant different (P<.05) in second week after treatment. Body protein content has significant different (P<.05) in first and second weeks after treatment. We concluded that the protease activity and body protein content decreased at deprivation, but increased after two weeks refeeding.

Aktivitas Amilase dan Laju Metabolisme Ikan Lele Dumbo (Clarias gariepinus) pada Kondisi Puasa dan Pemberian Pakan Kembali Esti Dwi Pratiwi; Untung Susilo; Slamet Priyanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.123

The study to determine amylase activity and metabolic rate of catfish, Clarias gariepinus under starvation and refeeding conditions has been conducted with three treatments and four replications in randomized completely design. The result showed that the amylase activity was significant different (P<.05) only at second week after treatment and metabolic rate was significant different (P<.050 at first and second week after treatment. Conclutions, the amylase activity and metabolic rate of catfish have been decreased at starvation, especially at second week, but it recovery at refeeding condition.

PENGGUNAAN EM4 DALAM PAKAN BUATAN UNTUK MENINGKATKAN KEEFISIENAN PAKAN DAN PERTUMBUHAN IKAN NILA GIFT (Oreochromis sp.) Farida Nur Rahmawati; Untung Susilo; Bambang Hariyadi
Agroland: Jurnal Ilmu-ilmu Pertanian Vol 13, No 3 (2006)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.563 KB)

The fish growth is influenced by the quality of food consumed. EM4 with difference doses (0, 5, 10, and 15 ml/kg diet) was carried out to investigate feed efficiency and the growth of nila gift. An experimental method based on completely randomized design in four replicates was used for 35 days. nila gifts with 12 – 18 g in body weight were used. Fishes were fed twice daily as much as 5% of their body weight and unfed one was taken an hour after feeding. The result showed that the differences of EM4 on diet did not affect feed efficiency and the growth of nila gift. However, EM4 suplementation on diet could increase its protein content.

Pengaruh Harga, Persepsi Risiko Dan Kepercayaan Terhadap Minat Beli Pada Ud. Tri Jaya Rizki Abadi Di Kabupaten Kediri Suharyati Suharyati; Enni Sustiyatik; M Dian Ruhamak; Untung Susilo; Wahyu Dede Gustiawan
RISK : Jurnal Riset Bisnis dan Ekonomi Vol. 2 No. 1 (2021)
Publisher : Universitas Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/risk.v2i1.1809

Penelitian ini bertujuan untuk mengetahui pengaruh harga, persepsi risiko dan kepercayaan terhadap minat beli senapan angin studi kasus pada konsumen UD. Tri Jaya Rizki Abadi. Jenis penelitian yang digunakan dalam penelitian ini adalah survei. Populasi pada penelitian ini adalah konsumen dari UD. Tri Jaya Rizky Abadi pada bulan November sebanyak 194 orang dan bulan Desember sebanyak 158 orang sehingga total 252 orang. Sampel pada penelitian ini sebanyak 146 orang. Hasil penelitian menunjukkan bahwa : harga berpengaruh positif terhadap minat beli, yang kedua persepsi risiko berpengaruh terhadap minat beli, yang ketiga kepercayaan tidak berpengaruh secara signifikan terhadap minat beli. Jika semua variabel diolah secara bersama-sama hasilnya positif.

Comparison of Reproductive Aspects of the Tropical Eel Anguilla bicolor (McClelland 1884) in Freshwater and Estuarine Habitats Rachmawati, Farida Nur; Susilo, Untung; Aya, Frolan
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 3 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.3.260-266

The tropical anguillid eel, Anguilla bicolor McClelland, experiences significant growth up to the adult size in both freshwater and estuarine ecosystems, encountering salinity gradients that may impact their reproduction. Therefore, this study aimed to investigate the reproductive aspects of A. bicolor collected from Serayu River (freshwater) and Segara Anakan (estuary), Cilacap, Central Java, during July 2020 field surveys. The examined parameters included eye index (EI), fin index (FI), gonadosomatic index (GSI), hepatosomatic index (HSI), and sex ratio of eel specimens in freshwater and estuary. Furthermore, the total length (TL) and body weight (BW) ranged from 260 - 630 mm and 18 - 419 g in freshwater, and 260 - 630 mm and 18 - 447 g in estuary. Significant differences (P<0.01) were observed in the reproductive characteristics between the two habitats (P<0.01), with higher eye index, fin index, gonadosomatic index, and hepatosomatic index in freshwater-caught eels than the values obtained in estuary inhabitants. The mean of these four parameters were 6.99 ± 2.98%, 4.08 ± 0.71%, 1.70 ± 1.13%, and 1.92% ± 1.07 in freshwater, while 3.48 ± 1.60%, 3.45 ± 0.479%, 1.12 ± 0.23%, and 0.28 ± 0.19% were found in estuary. The sex ratio was significantly affected by habitat, with female eels dominating in freshwater (62.5%) compared to estuary (32%). These results suggested that eels in freshwater habitats might exhibit early gonadal maturation compared to those in estuary.

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