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All Journal Natural Science: Journal of Science and Technology Biocelebes AGROLAND: The Agricultural Sciences Journal

I Nengah Suwastika

Tadulako University

Author-ID : 397868

Agriculture, Biological Sciences & Forestry Computer Science & IT

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INDUKSI KALUS TANAMAN KAKAO (Theobroma cacao L.) KLON SULAWESI 1 (S1) PADA MEDIUM MS DENGAN KOMBINASI HORMON 2,4-D, BAP DAN AIR KELAPA Wilma Wilma; Muslimin Muslimin; I Nengah Suwastika
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Kakao's seed that can result same plant better with it superior parent indispensable. One of alternative is with utilize vegetative organ origin seed that resulting through network culture tech by processes embriogenesis somatik. This research constitute startup phase in embriogenesis which is plant callus induction kakao. Research was performed on month of March until August 2013 at Network culture Laboratory, kehutanan's faculty. Tadulako's university. Eksplan who is utilized as staminodia of kakao's flower part Sulawesi clone 1. attempt it is arranged bases fledged Random Design (RAL) with 4 conducts and 3 time replicate. Each one unit experimentaling to utilize 10 eksplan. Conduct that dicobakan which is MS0 + 1ppm 2,4 - D. + 0,1ppm BAP + Coconut Water 15% (KW 1 ), MS0 + 1 ppm 2,4 - D. + 0,2ppm BAP + Coconut Water 15% (KW 2 ), MS0 + 2 ppm 2,4 - D. + 0,1ppm BAP + Coconut Water 15% (KW 3 ) and MS0'S medium + 2 ppm 2,4 - D. + 0,2ppm BAP + Coconut Water 15% (KW 4 ). Observed parameter is while its appearance callus, eksplan's percentage that results callus, and callus morphology. Preserve is done at dark place on temperature 26 ºC. Result observationaling to point out all conduct that dicobakan that menginduksi can kakao's callus. MS0'S medium + 2 ppm 2,4 - D. + 0,1ppm BAP + Coconut Water 15% (KW 3 ) constituting best media for menginduksi callus that marked by its appearance chromatic callus turns white, get intermediet's type, greater relative callus mass, uniform and active clefts to begin 14 days culture afterses. Keywords: MS, 2,4 - D., BAP, Coconut water, Callus induction, Staminodia, Theobroma cacao L.

KAJIAN ETNOBOTANI TUMBUHAN PANGAN PADA MASYARAKAT SUKU SEKO DI DESA TANAH HARAPAN KECAMATAN PALOLO KABUPATEN SIGI SULAWESI TENGAH Bill Yorsan Yonathan; I Nengah Suwastika; Ramadhanil Pitopang
Biocelebes Vol. 10 No. 1 (2016)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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A research entitled “Ethnobotanical Study of food Plant in the Seko ethnic group in Tanah Harapan Village, Sigi Regency, District Central Sulawesi” has been carried out from March to April 2015. The research objective was to obtain the information of Plant diversity, part of the plant used as food and how to use as food. The research was done by methods that is using interview technique to 40 respondents with quisioner sheet. The result showed that there were fifty five (55) plants species that used by the Seko ethnic group in Tanah Harapan Village. The highest percentage that used in the part of plants were 40% of fruit. The Seko ethnic group in Tanah Harapan village utilize plants as food in a way that it can be consumed directly or to go through the first processing is cooked, boiled, fried, baked and coconut milk added. Key word : Seko Ethnic, Tanah Harapan Village, Sigi Central Sulawesi, Ethnobotanical Food Plant.

GAMBARAN INDEKS MASSA TUBUH PENDERITA TUBERKULOSIS (TB) POSITIF YANG MELAKUKAN PENGOBATAN DI GERDUNAS TB PARU RUMAH SAKIT UNDATA PALU SULAWESI TENGAH Fadhalna Fadhalna; Ihwan Ihwan; I Nengah Suwastika
Biocelebes Vol. 11 No. 1 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research on the description of the patient's BMI (body mass index) of tuberculosis (TB) in the positive treatment of pulmonary tuberculosis at the unit of integrated movement prevention of tuberculosis Undata Hospital Palu has been implemented since April until September 2015. This research aimed to look at the dynamics of BMI of patients during treatment. The method used was the retrospective design patients who present within 2014, which qualified as research sample. There are 45 respondents of eligible subjects complete data up to the end of treatment. The results showed a changeof each category of BMI from the early phase to the final phase of treatment. In theearly phase of treatment, is very thin and thin BMI were 30 respondents fromcategory 1 and category 2, its hows that most patients with early diagnosis of tuberculosis have a BMI below normal. While in the early phase until the end of treatment showed a significant frequency changes in norma lBMI reaches 24.5% categorized 1 and 0.1% incategory 2. This is due to irregular treatment undergone by the patient from the early phase to the final phase of treatment. This indicates that the category 1 more treatment success which is marked by the increasing quality of BMI’s Patients return to normal. While in Category 2 does not show the amount of success, because with drawing treatment.

INDUKSI KALUS JARAK PAGAR (Jatropha curcas L.) PADA MEDIA MS DENGAN PENAMBAHAN BERBAGAI KONSENTRASI BAP (Benzyl Amino Purin) DAN 2,4-D (Dichlorophenoxy Acetic Acid) Haryati Baktiar; Muslimin Muslimin; I Nengah Suwastika
Biocelebes Vol. 11 No. 1 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This research was conducted from January to June 2015 at the Tissue Culture Laboratory, Faculty of Forestry, Tadulako University. The explant used in this study was the stem of seedling. Experimental was based on a completely randomized design (CRD) with 4 treatments, and 3 replication, with 3 explants on each unit. Growth Medias as treatments were H1 = MS0 + 0.5ppm BAP + 1.5 ppm 2.4-D; H2 = MS0 +0.5 ppm BAP + 2 ppm 2.4-D, H3 = MS0 + 0.5 ppm BAP + 2.5ppm 2.4-D, andH4 = MS0 + 0.5 ppm BAP + 3ppm 2.4-D. Parameters observation were time in callus appear, type of callus, color of callus, the weight of callus, morphology of callus cells. Callus was induched on those medium under dark condition at 26-280 C. The result of the research showed that all treatments can induced the callus. The Medium of ms0 + 0,5ppm BAP + 1.5 ppm of 2,4-D (H1) was the best media for callus induction, as it showed in short period of callus induction, the appearance of yellowish-white colors, highest in mass and volume of the callus cell, and showed uniform and active cell in proliferation.

UJIVIABILTAS INOKULUM JAMUR TIRAM PUTIH ( PLEUROTUS OSTREATUS ( JACQ) P. KUMM) DALAM BENTUK SEDIAAN CAIR Siti Fatmawati; Umrah Umrah; I Nengah Suwastika
Biocelebes Vol. 11 No. 1 (2017)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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The research aimed to know the best formulations of basic substrate inoculums white oyster mushrooms (Pleurotus ostreatus (Jacq) P. Kumm) in the best liquid forms and their viability on production media. The research was conducted at Laboratory Biotekchnology of Depertement of Biology Faculty of Matematics and Natural Sciences, starting from March until July 2015. The experimental designed used was a Completely Randomized Design (CRD) which consisted of seven treatment and three replications. The compositions of the treatment were the ratio among wood sawdust, rice brain, corn starch and calcium (g) in successive sequences as follows : P0 (0 : 50: 50 : 3); (P1 10 : 45 : 45 : 3); (P2 20 : 40 : 40 : 3); (P3 30 : 35 : 35 : 3); (P4 40 : 30 : 30 : 3); (P5 50 : 25 : 25 : 3); (P6 60 : 20 : 20 : 3). The planting media used in this research were production media. The observation parameters namely the ability fungal growth of inoculums that meets surface the media with viability a high. The result of the research showed that P6 was the best treatment among. In the viability of the inoculums grew well on media production surface with good viability at 8 days of incubation.

KEANEKARAGAMAN KUMBANG CERAMBYCID (Coleoptera: Cerambycidae) PADA PERKEBUNAN KAKAO DI TIGA TIPE HABITAT rahmat rahmat; I Nengah suwastika; fahri fahri
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Penelitian ini bertujuan untuk mengetahui keanekaragaman kumbang Cerambycid (Coleoptera: Cerambycidae) pada perkebunan kakao di tiga tipe habitat. Penelitian telah dilakukan mulai bulan Juni 2017 hingga April 2018. Koleksi sampel dilakukan menggunakan Atrocarpus trap pada 3 tipe habitat perkebunan kakao yaitu habitat kakao pinggiran hutan (KPH), habitat kakao yang tidak terawat (KTT) dan habitat kakao terawat (KT). Total diperoleh sebanyak 381 individu, yang tergolong dalam 5 tribe, 7 genus dan 13 spesies. Nilai indeks keanekaragaman (H') dan kemerataan (E) tertinggi pada habitat kebun kakao tidak terawat (KTT). Indeks kesamaan Bray-Curtis (IBC), menunjukkan kesamaan antara kebun kakao di pinggiran hutan (KPH) dengan kebun kakao terawat (KT). Hasil dari penelitian ini menunjukkan bahwa tiga jenis perkebunan kakao pada penelitian ini, sangat mendukung keberadaan kumbang cerambycid dan tidak memiliki pengaruh yang signifikan terhadap kehidupan mereka.

INVENTARISASI JENIS DAN TINGKAT KESEHATAN POHON PELINDUNG PADA BEBERAPA TAMAN KOTA PALU SULAWESI TENGAH Sahril M Taher; I Nengah Suwastika; Ramadhanil Pitopang
Biocelebes Vol. 12 No. 3 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research entitled” Inventory species and levels of demage Shade Tree at some Park in Palu Town, Central Sulawesi”, has been conducted from July to August 2015. The research was aimed to inventory of all shade tree species and to determine the levels of damage in four (4) Parks in Palu Town. The data analysis was done on the percentage of wound compact to the diameter of a main trunk and the percentage of the damaged on the trunk the result of the research showed that, there were six (6) species of shade tree belong to five (5) families with individual number seventy four (74) of Trembesi (Samanea saman (Jack.) Merr.), Thirty five (35) of Mahoni (Swetenia mahagoni (L.) Jacq), Twenty eigh (28) of Katapang (Terminalia catappa Linn), six (6) of Banyam (Ficus benjamina Linn), Four (4) of Johar (Senna seamea Lamk), Two (2) of Kayu Jawa (Lannea coromandelica (Houtt.) Merr). The highest percentage of damage on these four (4) ports was found on of Trembesi (Samanea saman (Jacq.) Merr), which grows in GOR City Park. The average of wounded as 2.71 % and the percentage of damage on stems was 20 %.

UJI BEBERAPA FORMULA INOKULUM TERHADAP PRODUKSI TUBUH BUAH JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq.) P.Kummer.) Dana Sulistia; Umrah Umrah; I Nengah Suwastika
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15086

The study of test for several inoculum formulas on the production of white oyster mushroom body (Pleurotus ostreatus (Jacq.) P.Kummer.) conducted from April to September 2018 at Biotechnology laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. This study were aimed to determine whether the treatment of the inoculum formula tested on the production medium can form the white oyster mushroom fruit body and to find out which inoculum formula can form the optimum fruit body in the production medium. This study was conducted by Randomized Complete Design (RAL) consisted of 4 treatments and four replications, I1 (100% corn seeds), I2 (50% : 23%: 27%), I3 (50% : 17% : 33%) dan I4 (95% : 5%). The results showed that I 3 was the best inoculum for the growth of the white oyster mushroom fruit body compared to the other inoculums which were characterized by a wide mushroom hood, the length was long and the weight of fresh mushroom was high. The inoculum formula and the best dosage for body production of white oyster mushroom are found in treatment I3 (50% sawdust + 17% corn flour + 33% bran).

FORMULASI MEDIA PRODUKSI JAMUR TIRAM (Pleurotus ostreatus) DENGAN SUPLEMENTASI AMPAS SAGU Sri Utami; Umrah umrah; I Nengah Suwastika
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15087

This study aims to know the basic media with sago pulp supplementation which can be formulated into a medium for producing white oyster mushrooms and also to know the comparison of the right media and supplements in the formulation to give maximum oyster mushrooms production. In this study using a comparison with sawdust substrate and sago pulp to see how effective the sago pulp substrate as a supplement media for the oyster mushroom production. the program used is experimental designed in Completely Randomized Design (CRD), consists of three replications so that twelve experiments were obtained. The treatment arrangement is M1: Base media without supplements (controls), M2: 90% base media + 10% sago pulp, M3: 80% basic media + 20% sago pulp, M4: base media 70% + sago pulp 30%. The observation parameters used in the study are mycelium growth, fruit body growth, biological efficiency and measurement of protein levels in the fruit body of the oyster mushroom. The results showed that the addition of sago pulp in the media of oyster mushroom production had no effect on the growth of oyster mushrooms. This can be seen from the media of production of M1 (without the addition of sago pulp) experiencing a higher growth rate of mycelium compared to other treatments. The addition of sago pulp has an effect on the levels of oyster mushroom protein. This can be seen from the protein content of oyster mushrooms in M2 production media which have high protein content. Normal levels of white oyster mushrooms in sawdust media range from 18% -20%. This is due to the mixing of wood powder as a production medium and 10% sago pulp as supplementation to produce more nutrient levels.

POTENSI EKONOMI PEMANFAATAN LIMBAH BATANG PISANG DAN LIMBAH JERAMI JAGUNG SEBAGAI SUBSTRAT DASAR UNTUK PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH umrah umrah; I Nengah Suwastika; Lina Mahardiana; Meryany Ananda; Novika
Biocelebes Vol. 15 No. 1 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i1.15490

Pertumbuhan miselium jamur tiram putih (Pleurotus ostreatus) pada substrat (batang pisang dan batang jagung) telah dilakukan di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam. Penelitian ini bertujuan untuk mengetahui: (a) pertumbuhan P. ostreatus pada substrat yang ditambahkan dengan limbah air kelapa dan limbah tempe; (b) karakteristik pertumbuhan P. ostreatus pada perlakuan berbagai jenis substrat. Penelitian ini dirancang berdasarkan Rancangan Acak Lengkap (RAL) yang terdiri dari enam perlakuan dan empat ulangan. Komposisi dari perlakuan adalah rasio antara batang pisang : batang jagung yaitu P1 (100%), P2 (80%: 20%), P3 (60%: 40%), P4 (40%: 60%), P5 (20% : 80%), P6 (100%). Hasil penelitian menunjukkan bahwa Perlakuan P6 lebih baik dibandingkan dengan perlakuan lain pada pertumbuhan miselium.

Co-Authors Agung, Muhammad Budi Akhmad Khumaidi Ali Djamhuri Anis Nurwidayati Anna Rufaida Aras, Mohammad Rezha Ari Sulistiami Asri Pirade Paserang Bill Yorsan Yonathan Cindy Claudia, Cindy Dana Sulistia Eny Yuniati Fadhalna Fadhalna Fahri Fahri Fahri Fahri Felma Tri Utami Frastika, Dian Gazali, Faris Muhammad Haliana Haliana Haliani Haliani Handayani, Mutia Harliana Harliana Haryati Baktiar Herlina Herlina Ihwan Ihwan Imelda, Silvia Jayawarsa, A.A. Ketut Jintan Jintan Laksono Trisnantoro Lina Mahardiana Marina Marina Mazidaturohmah, Mazidaturohmah Melisa S.M. Sorentina Meryany Ananda Mohammad Fahmi Musdalifah Musdalifah Muslimin Muslimin Ni Nyoman Lidyawati Niluh Made Dwi PYD Ningsi, Putri Suria Novika Nurul Aisyah Nurvita Nurvita Orryani Lambui Paserang, Asri P. Pranata, Adhi Rahmansyah Rahmansyah Rahmat Rahmat Ramadanil Pitopang Ramadhanil Pitopang Ramadhanil pitopang Ramadhanil Pitopang Rifka Rifka Riska Tajuddin Rosidah, Maya Sofiyanti Sahril M Taher Sari, Riska Sri Niken Ariati Sri Utami Talantan, Vrillia Marannu Umrah Umrah Urfiana Urfiana Waeniati Waeniati Waeniaty Waeniaty Weaniati Weaniati Wilma Wilma Yundari, Yundari Yunus, Fahrunnisa Yuzammi Yuzammi

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