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All Journal Jurnal Kimia Mulawarman JURNAL ATOMIK Jurnal Ilmiah Sains al Kimiya : Jurnal Ilmu Kimia dan Terapan
Winni Astuti
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Mulawarman University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Energy Environmental Science Mathematics Physics

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SKRINING BAKTERI DARI AIR SUNGAI KARANG MUMUS SEBAGAI PENGHASIL ENZIM PROTEASE, SELULASE DAN LIPASE Dhita Novalia Amantha; Winni Astuti; Eva Marliana
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1402

Abstract

Bacteria from polluted waters have the potential to produce extracellular hydrolase enzymes, namely protease enzymes, cellulase and lipase. This study was conducted to isolate bacteria from the water of the Karang Mumus River around Segiri Market, Samarinda as a producer of hydrolase enzymes. The bacteria were isolated in a nutrient-dense agar (NA) medium by the streak method. The results of bacterial isolation were obtained from 14 single colonies of bacteria. Single colonies of bacteria are selected to produce hydrolase enzymes in the form of proteases, cellulases and lipases. The results of the selection of hydrolase enzyme-producing bacteria were 3 protease-producing isolates, 10 cellulase-producing isolates, and 5 lipase-producing isolates.
SELEKSI AMILASE PENDEGRADASI PATI MENTAH BAKTERI DARI AIR GALIAN BEKAS TAMBANG BATU BARA DI DESA LOA BAHU Akbar Akbar; Winni Astuti; Djihan Ryn Pratiwi
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1546

Abstract

Raw starch-degrading amylase (RSDA) is a type of hydrolase enzyme that can degrade raw starch into glucose without going through the heating and gelatinization process. This study was conducted to select RSDA-producing bacteria from ex-coal mine water samples in Loa Bahu Village, Sungai Kunjang, Samarinda, with various types of raw starch such as rice, wheat, corn, potatoes, sago, and cassava. The selection of bacteria-producing RSDA enzymes was carried out using agar nutrient media containing raw starch, the positive test was marked by the formation of a clear zone around the bacterial colony after the addition of iodine solution. A total of 13 bacterial isolates (AT 1 - AT 13) were tested qualitatively by RSDA. The selection results showed that 4 bacterial isolates were capable of degrading raw rice starch, 4 bacterial isolates were capable of degrading raw corn starch, 2 bacterial isolate was capable of degrading raw cassava starch, 1 bacterial isolate was capable of degrading raw potato starch, 1 isolate was capable of degrading raw wheat starch, and 2 bacterial isolates were able to degrade raw sago starch. The bacterial isolate with the code AT3 is capable of degrading raw starch of rice, corn, potatoes, wheat and sago.
Co-Authors agita rachmala ginting Agung Prasetyo Akbar Akbar Anggun Ridha Avitri Asjayani Kurnia Sari Ayu Mellyanti Dhita Novalia Amantha Djihan Ryn Pratiwi Djihan Ryn Pratiwi, Djihan Ryn Erwin Erwin Eva Marliana Eva Marliana Eva Marliana, Eva M Nur Yasin Fahrul Fhozi Medi Hendra Prasetyo, Agung Putri, Amanda Aulia Rabiatul Adawiyah Rahmat Gunawan Rita Hairani Saibun Sitorus Saleh, Chairul Sherly Pricilia Subur P Pasaribu Sukamto, Ika Sumiyarsi Wihda Nisa Alhayyu