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Journal : FLUIDA

Aktivitas Bacillus subtilis pada Media Tulang Ayam dalam Pembentukan Gelatin Sudrajat Harris Abdulloh; Bintang Iwhan Moehady; Irwan Hidayatulloh
Fluida Vol 13 No 1 (2020): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v13i1.2221

Abstract

ABSTRAK Gelatin merupakan bahan tambahan yang banyak terdapat pada industri makanan, minuman, dan tekstil. Gelatin terbentuk secara kimia melalui proses asam dan basa atau dengan bantuan aktivitas mikroorganisme. Proses kimia pembentukan gelatin membutuhkan waktu antara satu sampai lima minggu, sedangkan proses dengan aktivitas mikroorganisme terbentuk antara 12 sampai 24 jam. Penelitian ini bertujuan untuk membuat gelatin dengan menggunakan bakteri Bacillus subtilis ATCC No. 21228 serta bahan baku tulang ayam. Aktivitas bakteri Bacillus subtilis akan merombak kolagen dalam tulang ayam menjadi gelatin. Kondisi operasi yang digunakan yaitu: temperatur 45, 50, dan 55°C; pH 7, 8, dan 9; dan konsentrasi 120 g/L, 160 g/L, 200 g/L dan 240 g/1. Analisis terhadap produk yang diperoleh menggunakan uji kandungan protein dan uji pembentukan gelatin. Hasil penelitian menunjukkan kondisi optimum terjadi pada pH = 9, T = 45°C, konsentrasi (C) = 200 g/L, dan konversi rata-rata = 72,25%. Kata kunci: Gelatin, Bacillus subtilis, limbah tulang ayam ABSTRACT Gelatin is an additive that is widely available in the food, beverage, and textile industries. It is formed chemically through acid and alkaline processes or with the help of microorganism activity. The chemical process for gelatin takes 1-5 weeks, while the process with microorganism activity occur only 12-24 hours. This study aims to make gelatin using the bacteria Bacillus subtilis ATCC No.21228 from raw material for chicken bones. The activity of the Bacillus subtilis bacteria will convert collagen in chicken bones into gelatin. The operating conditions used in this work were: temperature under 45, 50, and 55°C; pH between 7, 8, and 9; and concentrations of 120 g/L, 160 g/L, 200 g/L and 240 gr/1. The result tested to obtain the existing protein and gelatin content. The results showed that the optimum conditions occurred at pH = 9, T = 45°C, concentration = 200 g/L, and the average conversion = 72.25%. Keywords: Gelatin, Bacillus subtilis, chicken bone waste
Seeding and Acclimatization for Aerobic Processing of Restaurant Wastewater with Sequencing Batch Reactor Herawati Budiastuti; Laily Isna Ramadhani; Sudrajat Harris Abdulloh; Ananda Dea Maharani; Melina Melina; Robby Sudarman
Fluida Vol 16 No 1 (2023): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v16i1.4521

Abstract

Restaurant wastewater has a relatively high organic matter content, so it needs to be treated to meet the specified quality standard. One of the technologies that can be used in restaurant wastewater treatment is Sequencing Batch Reactor (SBR) technology. The purpose of this study is to set up an aerobic SBR system with seeding and acclimatization treatments to reduce the amount of organic matter in restaurant wastewater when a shock load occurs. The research was done using wastewater from a restaurant in Bandung and activated sludge from the food industry in Bogor as seeds for microorganisms. In this study, the seeding process was carried out by introducing 25% activated sludge and 75% nutrients into the reactor, and the acclimatization process was carried out by introducing a specific ratio of nutrients and wastewater into the reactor gradually until the waste concentration reached 100%. The parameters tested were COD, MLVSS, DO, pH, and temperature. During the seeding procedure, the initial COD value of 3,200 mg/L declined. It began to stabilize on the seventh day, with a COD value of 1,080 mg/L. The COD removal reached a relatively stable condition in the acclimatization process starting on day 2, where COD decreased from the original 1,280 mg/L to 480 mg/L.
Efektivitas Katalis Heterogen Fly Ash (FA-SO3H) pada Sintesis Biodiesel dari Palm Fatty Acid Distillate Abdulloh, Sudrajat Harris; Andrijanto, Eko; Saripudin, Saripudin; Putri, Anindya Indrita; Nurul, Dhea; Elizabeth, Lidya
Fluida Vol. 17 No. 1 (2024): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v17i1.5286

Abstract

The increase in fuel use along with the decline in production of petroleum exploration activities encourages efforts to find alternative fuels, one of which is biodiesel from PFAD (Palm Fatty Acid Distillate) with production reaching 6,000,000 tons while its utilization is still low. Biodiesel production from PFAD using Fly ash (FA)-SO3H catalyst which is a heterogeneous catalyst through esterification method. FA used in this study was wet impregnated using 6M sulfuric acid (H2SO4) for 24 hours, so that the highest acid concentration was obtained at a calcination temperature of 500 ⁰C of 1.1654 mmol/gram. The research was conducted with Response Surface Method (RSM) with Box Behnken Design model in Design Expert 13 software and 13 runs were obtained. The resulting model from RSM was obtained as optimum conditions at catalyst loading (%-w) 5-10, methanol:PFAD molar ratio (n/n) 10:1-30:1, reaction time 3-5 hours and temperature 60 ⁰C. Based on the experimental results and ANOVA, the high conversion is influenced by the high catalyst loading and methanol:PFAD ratio (n/n), with the conversion obtained of 72.89% at catalyst loading of 10%-w, methanol:PFAD molar ratio of 20 (n/n) and time for 5 hours.
The Effect of Plasma Electrolysis for Biodiesel Synthesis Using Waste Cooking Oil as a Raw Material and KOH Catalyst Shoelarta, Shoerya; Saripudin; Fedianto, Rahmat Dani Dwi; Darojat , Moch Adli; Abdulloh, Sudrajat Harris; Hidayatulloh, Irwan; elizabeth, lidya
Fluida Vol. 16 No. 2 (2023): FLUIDA
Publisher : Department of Chemical Engineering, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v16i2.3987

Abstract

The high consumption of cooking oil in Indonesia is directly proportional to the production of used cooking oil waste that can pollute the environment. To overcome this problem, used cooking oil is used as a biodiesel feedstock using the plasma electrolysis method. Plasma electrolysis is a synthesis process by applying a high voltage to the process. This study aims to determine the effect of the plasma electrolysis method with variations in voltage (400 - 500 volts) and the type of reagent (methanol and ethanol) on % yield and characteristics of biodiesel. The process is carried out by reacting used cooking oil with a reagent that is given a voltage until an electric discharge occurs and two layers are formed and the calculation of the specific energy requirements for the synthesis process is carried out. The results show that at a voltage of 500 volts with methanol reagent produces a yield of 85.73% with characteristics according to the SNI 7128:2015 standard such as density 888.36 Kg/m3, viscosity 5.79 Cst, acid number 0.39 mg - KOH/g, content ester 97.56% and flash point 117oC with the exception of water content 0.373%, with a specific energy requirement of 1758,031 J/mL.