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Journal : Nusantara Science and Technology Proceedings

Different Dietary Protein Sources Affect the Intestinal Tight Junctions in the Colon of Mice Rini , Dina Mustika; Pratiwi , Yunita Satya; Munarko , Hadi; Suzuki , Takuya
Nusantara Science and Technology Proceedings 1st International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0542

Abstract

This study examined the effects of some dietary protein sources on intestinal barrier function in the colon of mice. 24 female ICR mice were randomly grouped into 4 groups (n=6) fed casein, soybean protein, whey protein concentrate (WPC), and egg white diet for 28 days. In this study, we collected the colon to analyzed the TJs proteins. Cecal contents were also collected to analyze the SCFAs. Mice fed with WPC showed the highest serum LBP concentration. Soybean and casein groups had the highest expression of occludin in the colon of mice. Total SCFAs were not significantly different among the groups. Correlation analysis showed that there is a positive correlation between ZO-1 and ZO-2 with total SCFAs. There is a positive correlation between plasma LBP concentration with claudin-3 expression in the colon of mice. These results confirmed that various dietary protein intake has a significant impact on the regulation of intestinal barrier integrity and SCFAs production might be contributing to the observation.
Characteristics of Brown Rive var. Inpari 43 and Sonication Treatments for Extraction of Phenolic and Flavonoid Contents Hadi Munarko; Slamet Budijanto; Azis Boing Sitanggang; Feri Kusnandar
Nusantara Science and Technology Proceedings 1st International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0507

Abstract

This study aimed to characterize brown rice grain var. Inpari 43 and evaluate the effect of sonication time on total phenolic and flavonoid content. The result showed that Inpari 43 is classified as medium and slender grain which contains about 12% of moisture content. Maceration method was suitable for brown rice flour extraction. It accumulated 65–70 mg GAE/100g and 13–18 mg QE/100g of total phenolic content and flavonoid content, respectively, during 10 to 30 minutes extraction. The pearson correlation analysis indicated a strong positive correlation between extraction time and phenolic and flavonoid content. In conclusion, maceration for 30 minutes accumulates the highest phenolic and flavonoid content in brown rice flour var. Inpari 43.
Borax as a Non-Food Grade Addivite in the Perspective of Foof Safety and Human Resources Formation: A Literature Review Yunita Satya Pratiwi; Dina Mustika Rini; Hadi Munarko; Hadi Prayitno; Ristya Widi Endah Yani; Nadia Shoukat; Kaleem Ahmad
Nusantara Science and Technology Proceedings 1st International Conference Eco-Innovation in Science, Engineering, and Technology
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0518

Abstract

Many foodborne disease cases are still existing in Indonesia and worldwide. Chemicals that act as non-food grade additives is one of the cause. Borax is a chemical which still widely used for food in the community. The purpose of writing this scientific article is to analyze the impact of using borax as a non-food grade additive from the perspective of food safety and human resource formation. This article was based on scientific phenomena that occurred in studies conducted by the author in private, which was then analyzed more deeply based on a study of theories. As mentioned in government law, the use of borax for food additives is prohibited. However, borax is still widely used as food additives in the communities. This phenomenon is a threat to the assurance of food security for individuals and society, which in turn will have an impact on the output quality of human resources needed as the subject of future development. Several studies, investigated the negative effects of borax on the biological function of living things, showed that the use of borax significantly affects food safety in terms of human resources formation. Therefore, it needs both a preventive and curative solution for individuals, groups, and the government in an integrated manner. The use of borax in food is a threat to the assurance of food safety for individuals and society, which in turn will affect the quality of human resources so that a preventive and curative solution is needed.