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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) JPBIO (Jurnal Pendidikan Biologi) YARSI Medical Journal Health and Medical Journal JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Jurnal MedScientiae Jurnal Bioteknologi & Biosains Indonesia (JBBI) Cermin Dunia Kedokteran Jurnal Bioteknologi & Biosains Indonesia
Kris Herawan Timotius
Krida Wacana Christian University, Jalan Arjuna Utara 6, Jakarta 11510, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Jurnal MedScientiae

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Isolasi Bakteri Methicillin Resistant Staphylococcus aureus pada Gagang Pintu Gedung Kampus X di Jakarta Pasaribu, Donna Mesina Rosadini; Kelvin, Kelvin; Timotius, Kris Herawan
Jurnal MedScientiae Vol. 3 No. 1 (2024): April
Publisher : Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/JMedScientiae.v3i1.3062

Abstract

Bacteria are one of the component parts in every ecosystem. Staphylococcus aureus (S. aureus) is a normal surface flora of the human body, as a temporary contaminant on inanimate objects such as clothing, cutlery and doorknobs, but also a oportunistic even pathogen on the host. The mutated strain of S. aureus has a cross-resistant strain against all antibiotics of the Beta Lactam, Methicillin, Oxacillin and Flucloxacillin groups, called Methicillin Resistant Staphylococcus aureus (MRSA). This research is needed because MRSA is a strain of bacteria that can be everywhere, especially in public facilities, such as doorknobs, which can be a place of transmission (transfer) of bacteria from one host to another. The samples in this study were taken by swabbing from the door handle in building X, Jakarta. A total of 62 samples were then cultured on Mannitol Salt Agar (MSA) media, the growing isolate was tested by Gram staining, catalase test, and mannitol. Isolate suspected of Staphylococcus aureus, ascertained whether MRSA strain using 30 μg antibiotic C efoxitin (fox) disc. The results of this study showed that 58 (87%) isolates were Staphylococcus aureus and sensitive to C efoxitin antibiotics, 8 (13%) isolates, mnitol test negative, sensitive to Ceroxitin antibiotics. The study concluded that the bacterial isolate found was Staphylococcus aureus sensitive methicillin (MSSA), no MRSA was found.
Evaluasi Mikrovaskuler dengan Near Infrared Spectroscopy pada Pasien Kaki Diabetes Kartika, Ronald Winardi; Timotius, Kris Herawan
Jurnal MedScientiae Vol. 3 No. 1 (2024): April
Publisher : Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/JMedScientiae.v3i1.3074

Abstract

Peripheral Arterial Disease (PAP) requires good evaluation to reduce amputation rate. The aim of this study is to assess non-invasive detection methods in evaluating microvascular conditions in PAP. The potential use of Near Infrared Spectroscopy (NIRS) in identifying disorders in patients with PAP, either with or without DM, was also assessed. A systematic literature search was conducted through PUBMED until October 2023. Based on the literature review, it can be concluded that the use of NIRS in evaluating microvascular status in PAP is a non-invasive testing method that has expanded its role. Evaluation with NIRS provides metrics such as recovery time, deoxygenation, oxygen consumption (VO2), tissue oxygen saturation (StO2), total haemoglobin (HbT), and oxyhemoglobin area under the curve (O2 HbAUC). NIRS also has the potential to assist multidisciplinary teams in treating high-risk patients, especially in diabetic patients whose symptoms are compensated by peripheral neuropathy. This study shows that NIRS can be used as a useful tool in evaluating microvascular status in PAP, by providing important information that can be used to optimise patient care and prevent more serious complications. It is concluded that NIRS can be used as a tool to evaluate the effectiveness of treatment and prevent worsening of the condition of patients with PAP in diabetic foot.
Potensi Sekretom Mesenchymal Stem Cell pada Terapi Regeratif Penyakit Paru Fibrosis Kartika, Ronald Winardi; Timotius, Kris Herawan
Jurnal MedScientiae Vol. 3 No. 2 (2024): Agustus
Publisher : Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/JMedScientiae.v3i2.3142

Abstract

Fibrosis lung diseases such as chronic obstructive pulmonary disease, acute lung injury and post-Covid 19 fibrosis lung disease are considered as major health problems in the last decade. Cellular therapy with Mesenchymal Stem Cells (MSC) offers a novel therapeutic approach for fibrosis lung disease associated with anti-inflammatory, immunomodulatory, regenerative, pro-angiogenic and anti-fibrotic properties. Such therapeutic effects may be attributed to the MSC-secretome, which is made of free soluble proteins and extracellular vesicles (EVs). This review summarises some recent literature findings related to the efficacy and safety of MSC-derived products in pre-clinical models of lung diseases, demonstrating the biologically active substances contained in the MSC-secretome and its mechanisms involved in tissue regeneration. A perspective view is given on the secretome being a high-quality, safe and effective medicinal product,  
Co-Authors Ahmad Hamim Sadewa Donna Mesina Rosadini Pasaribu Flora Rumiati Hemi Sinorita Ignatio R. Haryono Ika Rahayu Kartika, Ronald Kartika, Ronald Winardi Kelvin Kelvin Margaretha Margretha, Margretha Puspasari, Monica Sancnia, Sancnia Santoso, Adit Widodo Santoso, Adit Widodo Sartika, Chyntia Retna Simamora, Adelina Steven, Michael Sudrajat, Susana Elya Teiseran, Virginia Marsella Tena Djuartina, Tena Veronika Maria Sidharta Wani Devita Gunardi