Yulianti, Novi Dwi
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Exploration of Pathogenic Microbes and Antagonistic Agents in Apple Production Centers in Malang Regency Abadi, Abdul Latief; Choliq, Fery Abdul; Trianti, Irisa; Maknunin, Lu'lu'il; Yulianti, Novi Dwi
Plantropica: Journal of Agricultural Science Vol. 9 No. 1 (2024)
Publisher : Department of Agronomy, Faculty of Agriculture, Brawijaya University

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Abstract

The apple farming group is one of the built of Department of Plant Pests and Diseases, Agricultural Faculty, Brawijaya University. In 2019, this departmen received a request from apple farmers in Madiredo Village, Pujon District to held an assistance programs of apple cultivation and environmentally pest control. This is related with the increasing of fruit rot and other diseases which is caused a decrease of apple production especially in Malang Regency. In general, apple farmers still rely on disease control using chemical fungicides which can have a negative impact on the environment and create resistance to pathogenic fungi. Therefore, this research was carried out to explore and study the potential of biological agents in apple production centers in Malang Regency, specifically in Poncokusumo, Pujon, and Batu District on conventional fields and mixed fields. This research was held observationally through fields surveys and farmer interviews, took soil samples and disease samples then then isolating, identifying and testing for antagonism at laboratory. Based on interviews and observation of symptomology, two dominant diseases were found, namely fruit rot disease (Gloeosporium sp.) and marsonina leaf spot disease (Marssonina coronaria). The results of exploration of the rhizospheric apple plants obtained isolates on conventional fields in Kec. Pujon has the highest abundance of fungal colonies compared to other regions. Based on the results of the antagonism test for pathogenic fungi and biological agents, it was found that the isolate codes KH.C, KK.A, JK.B, JK.J, and BK.B had the highest percentage of inhibition or the most effective in controlling the pathogen Gloeosporium sp. So in the next, the species can be identified molecularly.
Efficacy of Biofungicide with Active Ingredients Trichoderma sp. Against Late Blight Disease (Phytophthora infestans) in Potato Plants Abadi, Abdul Latief; Trianti, Irisa; Choliq, Fery Abdul; Sektiono, Antok Wahyu; Yulianti, Novi Dwi
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1591

Abstract

The cultivation of potatoes in Indonesia faces challenges, particularly a decline in production attributed to the devastating impact of late blight caused by the fungus P. infestans. Traditional control methods involve the frequent use of chemical pesticides, which pose environmental and health risks. To address this, the research aimed to assess the efficacy of biofungicide containing Trichoderma sp. as a biological agent in controlling late blight on potatoes. The study, conducted in Sumberbrantas Village, Bumiaji District, Batu City, East Java, and the plant disease laboratory at Brawijaya University from January to April 2023, employed both in vivo and in vitro experiments. A Randomized Block Design (RBD) was used for in vivo research, while a Completely Randomized Design (CRD) was employed for in vitro studies, each comprising five concentration treatments with five repetitions. Parameters such as disease intensity, efficacy level, potato production, and biofungicide inhibition were observed. Analysis of variance and the Tukey Honestly Significant Difference (HSD) test were applied to the data and processed using Microsoft Excel. The results indicated that biofungicide with Trichoderma sp. concentrations of 6 ml/l, 4.5 ml/l, 3 ml/l, and 1.5 ml/l effectively reduced the intensity of late blight on potatoes, with efficacy levels exceeding 50%. The highest potato production occurred with a concentration of 6 ml/l, yielding 15.44 tons/ha, followed by 14.67 tons/ha for the 4.5 ml/l concentration. The biofungicide with a concentration of 6 ml/l exhibited the lowest disease intensity at 14.28% and the highest efficacy at 83.08%. Inhibition tests demonstrated that concentrations of 6 ml/l and 4.5 ml were highly effective, inhibiting P. infestans growth by an average of 62.04% and 59.90%, respectively. These findings highlight the potential of biofungicide with Trichoderma sp. in managing late blight on potatoes, providing a sustainable and environmentally friendly alternative to chemical pesticides.
Stabilitas Hasil 30 Genotip Padi (Oryza sativa L.) di Tiga Lokasi Yulianti, Novi Dwi; Waluyo, Budi
Produksi Tanaman Vol. 6 No. 9 (2018)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

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Abstract

Uji multilokasi memegang peranan penting dalam pemuliaan tanaman dan penelitian lainnya di bidang agronomi. Tujuan dari penelitian ini ialah untuk mempelajari interaksi genotip x lingkungan dan mendapatkan genotip padi hibrida yang mempunyai hasil dan stabilitas lebih tinggi. Penelitian dilakukan di  lahan sewa milik PT. Bisi International, Tbk Farm Kambingan, Kediri. Bahan yang digunakan ialah 30 genotip padi yang terdiri dari 20 genotip padi hibrida yang diuji, 5 genotip pendamping, dan 5 genotip  pembanding. Menggunakan rancangan acak kelompok. Perlakuan berupa 30 genotip padi diulang 3 kali. Analisis varians dilakukan pada data yang di peroleh di setiap lokasi, uji homogenitas kuadrat tengah galat yang dilanjutkan dengan analisis varians gabungan. Perbedaan penampilan genotip diuji menggunakan least significant increases 5%. Keragaman disebabkan oleh lokasi, genotip, dan Interaksi genotip x lingkungan. Genotipe dengan nilai koefisien regresi lebih dari 1 maka akan beradaptasi dengan baik pada lingkungan optimal, dan bila koefisien regresi kurang dari 1 maka beradaptasi baik pada lingkungan marginal. Terdapat 8 genotip padi hibrida yang mempunyai hasil stabil dan beradaptasi luas, dan tiga genotip padi hibrida yang beradaptasi pada lingkungan produktif. Padi hibrida yang mempunyai penampilan stabil dan di atas rata-rata umum, yaitu HY 1617 RA 001 (7.1 t/ha), HY 1617 RA 003 (6.9 t/ha), HY1617RA010 (7.5 t/ha), HY 1617 RA 011 (7 t/ha), HY 1617 RA 012 (7.4 t/ha), HY 1617 RA 014 (7.7 t/ha), HY 1617 RA 015 (7.2 t/ha), dan HY 1617 RA 019 (7.5 t/ha). Padi hibrida yang beradaptasi pada lingkungan produktif ialah HY 1617 RA 016 (7.1 t/ha), HY 1617 RA 017 (7.1 t/ha), dan HY 1617 RA 020 (7.5 t/ha).Uji multilokasi memegang peranan penting dalam pemuliaan tanaman dan penelitian lainnya di bidang agronomi. Tujuan dari penelitian ini ialah untuk mempelajari interaksi genotip x lingkungan dan mendapatkan genotip padi hibrida yang mempunyai hasil dan stabilitas lebih tinggi. Penelitian dilakukan di lahan sewa milik PT. Bisi International, Tbk Farm Kambingan, Kediri. Bahan yang digunakan ialah 30 genotip padi yang terdiri dari 20 genotip padi hibrida yang diuji, 5 genotip pendamping, dan 5 genotip pembanding. Menggunakan rancangan acak kelompok. Perlakuan berupa 30 genotip padi diulang 3 kali. Analisis varians dilakukan pada data yang di peroleh di setiap lokasi, uji homogenitas kuadrat tengah galat yang dilanjutkan dengan analisis varians gabungan. Perbedaan penampilan genotip diuji menggunakan least significant increases 5%. Keragaman disebabkan oleh lokasi, genotip, dan Interaksi genotip x lingkungan. Genotipe dengan nilai koefisien regresi lebih dari 1 maka akan beradaptasi dengan baik pada lingkungan optimal, dan bila koefisien regresi kurang dari 1 maka beradaptasi baik pada lingkungan marginal. Terdapat 8 genotip padi hibrida yang mempunyai hasil stabil dan beradaptasi luas, dan tiga genotip padi hibrida yang beradaptasi pada lingkungan produktif. Padi hibrida yang mempunyai penampilan stabil dan di atas rata-rata umum, yaitu HY 1617 RA 001 (7.1 t/ha), HY 1617 RA 003 (6.9 t/ha), HY1617RA010 (7.5 t/ha), HY 1617 RA 011 (7 t/ha), HY 1617 RA 012 (7.4 t/ha), HY 1617 RA 014 (7.7 t/ha), HY 1617 RA 015 (7.2 t/ha), dan HY 1617 RA 019 (7.5 t/ha). Padi hibrida yang beradaptasi pada lingkungan produktif ialah HY 1617 RA 016 (7.1 t/ha), HY 1617 RA 017 (7.1 t/ha), dan HY 1617 RA 020 (7.5 t/ha).