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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences e-NERS e-CliniC Jurnal e-Biomedik JURNAL BIOMEDIK JURNAL KEDOKTERAN KOMUNITAS DAN TROPIK Jurnal Keperawatan YARSI Medical Journal Jurnal Promotif Preventif Medical Scope Journal (MSJ) KESANS : International Journal of Health and Science Grimsa Journal of Science Engineering and Technology Jurnal Inovasi Global
Tuda, Josef Sem Berth
Universitas Sam Ratulangi
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Arifin, Samsul; Fitri, Loeki Enggar; Sujuti, Hidayat; Hermansyah, Bagus; Endharti, Agustina Tri; Burhan, Niniek; Candradikusuma, Didi; Sulistyaningsih, Erma; Tuda, Josef Sem Berth; Zein, Umar
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
Co-Authors Abdul-Hamid, Nur A Agustina Tri Endharti Angle M. H. Sorisi Angle Sorisi Bagus Hermansyah Bakhtiar, Muh. Alvahrefi Bernadus, Janno Berty Bradly Burhan, Niniek C. Nagere, Andrew Cecilia S. Akay Cindy A. Batti Cindy Sandra D. Pijoh, Victor Didi Candradikusuma Erma Sulistyaningsih Finka Tangel, Finka Firra, Andi H. F. Greta J. P. Wahongan Herman Warouw Hidayat Sujuti Hiroyuki Matsuoka Ivan T. Sigarlaki, Ivan T. J. Kepel, Billy J. Runtuwene Jein Olifia Kapantow Kristin R. Walangare Labaiga, Natasya G.E. Loeki Enggar Fitri Luis, Renjer Lumbantobing, Greva R. I. M. H. Sorisi, Angle Megaria Rompas Meilany Tangkilisan Melisa Pantow Mengko, Stevano Mesaulawan, Septyarini Monica Thiosanto Muhammad Fikri Nakatsu, Masami Numansyah, Muhamad Patris J. Lombogia Rading, Fabio B. Ram Marnex Tampilang Regina C. Tawas Rianna J Sumampouw Rina Kundre Samsul Arifin Sartika, Sitha Seroan, Alfa Yoan Sorisi, Angle Maria Hasthee Sorisi, Angle Maria Hesthee Stefany P. Kristin, Stefany P. Stella E. Lumiu Sumual, Rama L. Tati Ponidjan Ticoalu, Eliza Rolanda Trovancia, Grace Umar Zein Umbas, Irene Megawati Van Basten, Juan Victor D. Pijoh Wahongan, Greta J.P. Wahongan, Greta Jane Pauline Wijaya, Raditya Putri Yohanes C. W. Kawulur