Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

0.92

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia The Journal of Experimental Life Sciences (JELS) Agrointek Jurnal Pangan dan Agroindustri Jurnal Keteknikan Pertanian Tropis dan Biosistem Jurnal Teknologi Pertanian agriTECH Indonesian Journal of Biotechnology UNEJ e-Proceeding Chimica et Natura Acta Agroindustrial Technology Journal Menara Perkebunan

AGUSTIN KRISNA WARDANI

Universitas Brawijaya

Author-ID : 412509

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience

Published : 64 Documents Claim Missing Document

Claim Missing Document

Articles

Title

Optimasi Fermentasi Bagas Tebu oleh Zymomonas mobilis CP4 (NRRL B-14023) untuk Produksi Bioetanol Atmiral Ernes; Lia Ratnawati; Agustin Krisna Wardani; Joni Kusnadi
agriTECH Vol 34, No 3 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Second generation bioethanol can be produced from fermentation of natural renewable materials, such as agricultural crops, as well as from industrial and domestic waste. The present study was aimed to optimize the fermentation process (inoculum concentration, urea concentration, and fermentation time) for ethanol production from sugarcane bagasse by Zymomonas mobilis CP4 using response surface methodology (RSM) central composite experimental design (CCD). The RSM model predicted the optimum value of ethanol content was 1.257% (v/v) at inoculum concentration 15% (v/v), urea concentration 0.3% (w/v), and fermentation time 45 h. Based on the experiment, the ethanol concentration was 1.213% (v/v), which was in close agreement with the predicted value. Ethanol yield of this experiment was 0.479 with fermentation efﬁ ciency of 93.9%. The results presented here proved a signiﬁ cant contribution of Z. mobilis CP4 to the production of bioethanol from sugarcane bagasse.ABSTRAKBioetanol generasi kedua dapat diproduksi dari fermentasi bahan terbarukan, seperti produk hasil pertanian, dan limbah atau hasil samping pengolahan industri dan rumah tangga. Tujuan penelitian ini adalah optimasi parameter fermentasi yang meliputi konsentrasi inokulum, konsentrasi urea, dan lama fermentasi untuk produksi etanol dari bagas tebu oleh Zymomonas mobilis CP4 dengan menggunakan response surface methodology (RSM) central composite experimental design (CCD). Kondisi respon yang optimal berdasarkan prediksi model diperoleh pada konsentrasi inokulum 15% (v/v), konsentrasi urea 0,3% (b/v), dan lama fermentasi 45 jam, dengan prediksi respon kadar etanol sebesar 1,257% (v/v). Berdasarkan hasil penelitian, kadar etanol optimal diperoleh sebesar 1,213% (v/v), yang menunjukkan hasil yang tidak berbeda jauh dengan prediksi model. Yield etanol yang diperoleh sebesar 0,479 dengan eﬁ siensi fermentasi 93,9%. Hasil penelitian ini membuktikan bahwa strain bakteri Zymomonas mobilis CP4 memiliki potensi yang cukup menjanjikan sebagai mikroba penghasil etanol.

Produksi Etanol dari Tetes Tebu oleh Saccharomyces cerevisiae Pembentuk Flok (NRRL – Y 265) Agustin Krisna Wardani; Fenty Nurtyastuti Eka Pertiwi
agriTECH Vol 33, No 2 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The potential use of sugar cane molasses by flocculant Saccharomyces cerevisiae in ethanol production was investigated. In order to minimize the negative effect of calcium on yeast growth, pretreated sugar cane molasses with dilute acid was performed. The influence of process parameters such as sugar concentration and inoculum concentration were evaluated for enhancing bioethanol production. Result showed that maximum ethanol concentration of 8,792% (b/v) was obtained at the best condition of inoculum concentration 10% (v/v) and sugar concentration 15% (b/v). Based on the experimental data, maximum yield of ethanol production of 65% was obtained. This result demonstrated the potential of molasses as promising biomass resources for ethanol production.ABSTRAKEfisiensi produksi bioetanol diperoleh melalui ketepatan pemilihan jenis mikroorganisme, bahan baku, dan kontrol proses fermentasi. Alternatif proses untuk meminimalisasi biaya produksi etanol adalah dengan mengeliminasi tahap pemisahan sentrifugasi sel dari produk karena memerlukan biaya instalasi dan biaya perawatan yang tinggi. Proses sentrifugasi merupakan tahapan penting untuk memisahkan sel mikroba dari medium fermentasi pada produksi bioetanol. Untuk meminimalisir biaya produksi akibat proses tersebut digunakan inokulum Saccharomyces cerevisiae pembentuk flok dan tetes tebu sebagai sumber gula. Penelitian ini bertujuan untuk mendapatkan konsentrasi penambahan inokulum Saccharomyces cerevisiae pembentuk flok dan konsentrasi sumber gula dalam tetes tebu yang tepat dalam produksi etanol yang maksimum. Saccharomyces cerevisiae sebanyak 5%, 10%, dan 15% (v/v) diinokulasikan pada medium tetes tebu hasil pretreatment dengan kandungan gula 15%, 20%, dan 25% (b/v) pada pH 5. Fermentasi dilakukan pada suhu 30°C dan agitasi 100 rpm selama 72 jam. Etanol tertinggi didapat pada kondisi konsentrasi inokulum 10% (v/v) dan konsentrasi sumber gula 15% (b/v) yaitu sebesar 8, 792% (b/v) dengan yield etanol sebesar 65%.

Identifikasi Gen Transgenik pada Produk Susu Bubuk Kedelai dan Susu Formula Soya dengan Metode PCR (Polymerase Chain Reaction) Agustin Krisna Wardani; Annisa Alirsyah; Ana Fauziah
agriTECH Vol 37, No 3 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The need of soybean that reached up to 2,2 million tons per year has made Indonesia imports this commodity 1,62 million tons from countries that adopt Genetically Modified (GM) soybean. By the presence of GM soybeans in Indonesia, Genetically Modified Product (GMP) labelling has need to be done. Detection of GMP can be done by using PCR. The aim of this study was to determine the presence of trangenic genetic material in soy milk powder and soy formula products to classify as GMP. Another goal was to determine the optimum annealing temperature of the primers used. Based on this study, the optimum annealing temperature of the CaMV 35S primer and the EPSPS-CP4 primer was 60oC and 59oC. The NOS terminator primer’s optimum annealing temperature was not found. 6 soy milk powder samples and 5 soy formula samples are might be determined to be using transgenic soybeans due to the presence of EPSPS-CP4 genes and CaMV 35S promotor genes. Therefore, those 11 samples were classified as GMP.ABSTRAK Kebutuhan kedelai yang mencapai 2,2 juta ton/tahun memaksa Indonesia mengimpor sebanyak 1,62 juta ton. Sebagian besar kedelai impor berupa kedelai transgenik. Dengan munculnya kedelai transgenik di Indonesia, perlu adanya pelabelan Produk Rekayasa Genetika (PRG) untuk memenuhi hak-hak konsumen. Teknik yang dilakukan untuk mendeteksi PRG salah satunya menggunakan metode PCR. Penelitian ini bertujuan untuk mengetahui ada tidaknya gen transgenik pada produk susu bubuk kedelai dan formula soya, sehingga produk dapat digolongkan sebagai PRG atau tidak. Selain itu juga bertujuan untuk mengetahui suhu annealing optimum pada primer yang digunakan. Hasil penelitian didapatkan suhu annealing optimum primer CaMV 35S promotor adalah 60oC. Sedangkan untuk primer gen EPSPS-CP4 suhu annealing optimumnya 59oC. Untuk primer NOS terminator suhu annealing optimum tidak ditemukan. Dari amplifikasi DNA sampel, 6 sampel susu bubuk kedelai dan 5 sampel formula soya terdapat sisipan gen EPSPS-CP4 dan gen Promotor CaMV 35S. Dengan demikian 11 sampel tersebut dapat dikatakan sebagai PRG. Kata kunci: Produk rekayasa genetika; PCR; formula soya; susu kedelai bubuk; kedelai transgenik

Studi Komparasi: Produksi Bioetanol Nira Batang Kelapa Sawit oleh Flokulan dan Non- Flokulan Saccharomyces cerevisiae Kafidul Ulum; Indria Purwantiningrum; Retno Dwi Yustina; Untung Murdiyatmo; Agustin Krisna Wardani
agriTECH Vol 40, No 4 (2020)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Two types of yeast were used for bioethanol production from oil palm trunk sap, the flocculant Saccharomyces cerevisiae NCYC1195 and nonflocculant Saccharomyces cerevisiae Kyokai 7 (NCYC-479). Flocculant Saccharomyces cerevisiae is yeast that has ability to aggregate into flocks which precipitate rapidly in culture medium. The effect of urea as a nitrogen source was also investigated in this study. Some concentrations of urea were added i.e. 0%, 0.1%, 0.2%, and 0.3% (w/v) during fermentation. The purpose of this study is to obtain the best condition by strain of Saccharomyces cerevisiae and urea concentration for the highest ethanol production. The highest ethanol production and yield was obtained at 4.86% (v/v) and 0.52 (g/g) respectively, by nonflocculant Saccharomyces cerevisiae Kyokai 7 (NCYC-479) without the addition of urea.

Effect of Sucrose and Starter on the Chemical and Organoleptic Properties of Fig Leaf Tea Water Kefir SY, Muhammad Zakwan Saputra; Wardani, Agustin Krisna
agriTECH Vol 44, No 4 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.82628

Water kefir is a fermented drink made by adding water kefir grains to a water solution containing dried fruit or sugar. This research aims to determine the effect of sucrose, starter, and interactions on the chemical characteristics (antioxidant activity, total acid, medium pH) and organoleptics of fig leaf tea water kefir and to determine the interaction of antioxidant activity with total acid and medium pH. The experiment used variations in starter concentration (5% and 8%) and sucrose concentration (3%, 6%, 9% and 12%). Water kefir was analyzed through chemical analysis (antioxidants, total acid, medium pH, and sugar content) and organoleptic tests (flavor, aroma, color, and overall preference). The results showed that variations in the addition of sucrose, starter, and interactions influenced the metabolic results (primary and secondary metabolites) of microorganisms in water kefir grains and statically had a significant effect on changes in chemical and organoleptic properties (flavor, aroma, and overall preference). The best chemical characteristic parameters were obtained in the B2S3 treatment (8% starter + 9% sucrose) with antioxidant activity of 51.27%, total acid of 0.70%, and medium pH of 3.53. Meanwhile, the best organoleptic parameters were obtained in the B2S4 treatment (8% starter + 12% sucrose) with a taste value of 5.56 (liked), an aroma value of 4.80 (slightly liked), a color value of 4.97 (slightly liked), and the overall liking level was 5.45 (liked), and the antioxidant activity of fig leaf tea water kefir had a strong relationship with total acid and medium pH. Furthermore, the addition of sucrose and different starters during the water kefir fermentation process affects the chemical and organoleptic properties of fig leaf tea water kefir. It is hoped that the results of this research will make fig leaf tea water kefir an alternative probiotic drink for sufferers of lactose intolerance and allergies to milk-based drinks.

Co-Authors - Fadlurrahman, - . Harijono Adi Syamsuri Agustin Krisna Wardhani Ajeng Astrini Brahmanti AJI SUTRISNO Aji Sutrisno Aji Sutrisno Ajrullah, Mauludy Jutta Ana Fauziah Anita Fitri Astuti Anita Fitri Astuti Anita Kusuma Finalissari Annisa Alirsyah Annisa Fadlilah Koos Cahyani Annisa Fadlilah Koos Cahyani Asma' Khoirun Nisa Atmiral Ernes Azmi Nahdhiyati Fathimah Azmy Nahdhiyati Fathimah, Azmy Nahdhiyati BAMBANG SUSILO Budiasih Wahyuntari Christina Ekawati Halim Dadang Suhendar Deden Eris Devi arianty Dian Widya Ningtyas Dwi Arinda Syahputri Dwi Arinda Syahputri Dwi Okta Indriani Dwi Okta Indriani Efendi Oulan Gustav Hakim Nata Buana Eko Sutrisno Hawusiwa Eko Sutrisno Hawusiwa ekwan nofa wiratno Ekwan Nofa Wiratno Ekwan Nofa Wiratno Elok Puji Kurnia Sari Endrika Widyastuti Erni Sofia Murtini Erryana Martati Fabryana Noor Anggita Putri Fabryana Noor Anggita Putri Fadeli Muhammad Habibie Faula Libna Nabela Fenty Nurtyastuti Eka Pertiwi Feronika Heppy Sriherfyna Fithri Choirun Nisa Grace Wijaya Gunawan, Ellen Fenix Halimatus Sa'diyah Hardanti, Sri Harsojo Harsojo Harsojo Harsojo Harsojo Harsojo Helmy Aditya Prabowo, Helmy Aditya Husna, Afifa Ika Rachmawati Wardani Ika Rachmawati Wardani Ika Yuli Andarti Ika Yuli Andarti Indah Kusumawardini Indria Purwantiningrum IS HELIANTI Is Helianti IS HELIANTI Jamhari Jamhari Joni Kusnadi Kafidul Ulum Kezia Abib Yerah Tjandra Kharisma Nafia Safitri Laili One Januarista Lauren Chrisya Wiguna Lia Oriana Nindita Lia Ratnawati Luqvia Noer Islami Syamsudin Maria Ulfah Marisa Zakiya Ulfa Marisa Zakiya Ulfa, Marisa Zakiya Mochamad Nurcholis Muhamad Taufiqul Naufal Muhamad Tommy Adrian Muhamad Tommy Adrian Muhammad Nur Sigit Harianto Muhammad Nur Sigit Harianto Niknik Nurhayati NIKNIK NURHAYATI Nur Ida Panca Nugrahini Pramesti, Nadya Shafa Pratidina Andayani Prawiro, Sumarno Reto Prestasia Budi Lestari Reno Nasrudin Salas Retno Dwi Yustina Rhytia Ayu C. Putri Riris Wahyuhapsari Riris Wahyuhapsari Risqia Adinda Putri RR. Ella Evrita Hestiandari RUTH CHRISNASARI RUTH CHRISNASARI Silvy Novita Antrisna Putri Silvy Novita Antrisna Putri, Silvy Novita Antrisna Sindy Hamadi Suharjono Suharjono Suminar Diyah Nugraheni SY, Muhammad Zakwan Saputra TATI NURHAYATI Tri Ardyati Tri Yudani MR UNTUNG MURDIYATMO Untung Murdiyatmo Venisa Yosephi Vicha Vitalaya Masduki Vindy Irmanita Vindy Irmanita, Vindy Widya Dwi Rukmi Putri Windra Prayoga Yuliandri, Rahmat

Title Search

Found 5 Documents Search Journal : agriTECH

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search
Journal : agriTECH