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Journal : Life Science

PENGGUNAAN EM4 DAN MOL LIMBAH TOMAT SEBAGAI BIOAKTIVATOR PADA PEMBUATAN KOMPOS W, Deasy Amalia; Widiyaningrum, Priyantini
Life Science Vol 5 No 1 (2016): April 2016
Publisher : Universitas Negeri Semarang

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Abstract

Penggunaan bioaktivator dalam proses pengomposan berfungsi untuk mempercepat degradasi bahan organik, sehingga diharapkan mempercepat waktu terbentuknya kompos dengan kriteria yang diinginkan. Tujuan penelitian ini untuk : (a) mengetahui fluktuasi suhu, kelembaban dan pH harian pada proses pengomposan menggunakan bioaktivator MOL limbah tomat dan EM4, serta (b) mengetahui kualitas fisik dan kimia kompos yang dihasilkan. Penelitian didesain eksperimen lapang menggunakan rancangan acak dua perlakuan dengan 10 ulangan pengamatan. Perlakuan yang dicobakan adalah penggunaan bioaktivator MOL limbah tomat dan EM4. Bahan baku kompos terdiri dari sampah daun dan kotoran kambing dengan perbandingan 3:2, dan proses pengomposan berlangsung selama 3 minggu. Data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa parameter fisik kompos kedua perlakuan memperoleh skor 30 pada masing-masing kriteria warna,bau dan tekstur. Parameter kimia (kadar air, pH, C/N rasio, P2O5 dan K2O) pada kompos dengan MOL limbah tomat berturut-turut : kadar air 58,3%; pH 7,26, C/N rasio 13,98, P2O5 0,38 dan K2O 0.05). Penelitian ini menyimpulkan bahwa berdasarkan grafik fluktuasi suhu, kelembaban dan pH harian menunjukkan proses pengomposan berlangsung normal, Dalam waktu 3 minggu, parameter fisik (warna, bau, tekstur) dan parameter kimia khususnya C/N rasio kompos telah memenuhi standar kualitas menurut kriteria SNI 19-7030-2004. Utilization of bioactivator in the composting process aims to accelerate the degradation of organic materials, so the compost is mature more quickly and according to the criteria expected. The purpose of this study was to: (a) know the daily fluctuations in temperature, humidity and pH on the composting process using local microorganisms (MOL) of tomatoes waste and EM4, and (b) know the physical and chemical quality of the compost produced. This study was field experiment using randomized design with two treatments and 10 replications. The treatment is applied is the addition of bioactivator MOL of tomatoes waste and EM4. Compost material consisting of leaves garbage and goat manure with ratio of 3 : 2. The composting process is conducted for 4 weeks. The data were analyzed descriptively. The results showed that physical parameters of compost (colour, smell and texture) of both treatment obtained a score of 30 on each criteria. The chemical parameters on the compost with MOL of tomatoes waste, respectively: water content 58.3%; pH 7.26, C/ N ratio 13.98, P2O5 0.38%. and K2O 0.05%). The study concluded based on the graph daily fluctuations in temperature, humidity and pH, indicating that the composting process is running normally. Within 4 weeks, the physical and chemical parameters of the compost has fulfilled quality standards according to the criteria of SNI 19-7030-2004.
Viabilitas Dua Isolat Lokal Nematoda Entomopatogen pada Berbagai Variasi pH Media dan Uji Mortalitasnya terhadap Rayap Tanah Satria, Arif Bayu; Widiyaningrum, Priyantini; Ngabekti, Sri
Life Science Vol 7 No 1 (2018): April 2018
Publisher : Universitas Negeri Semarang

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Abstract

Nematoda entomopatogen (NEP) adalah suatu mikroorganisme yang berbentuk cacing berukuran 700-1200 mikron dan berada di dalam tanah. NEP isolat lokal mempunyai karakter ekologis berbeda-beda di setiap wilayah. pH memberikan pengaruh yang nyata terhadap mortalitas NEP. Informasi tentang faktor ekologis terutama toleransi terhadap pH media simpan yang belum diketahui. Tingginya penggunaan pestisida sintetik dapat mengakibatkan hal buruk bagi lingkungan. Agen pengendali hayati NEP memiliki nilai patogenitas yang tinggi terhadap rayap tanah. Tujuan penelitian ini adalah mengetahui pH media optimum terhadap viabilitas NEP dua isolat lokal dan mengetahui pengaruh dosis terhadap mortalitas rayap tanah selama 24 jam. Sampel yang digunakan adalah NEP isolat Semarang dan isolat Jember. Isolasi NEP menggunakan ulat hongkong kemudian di panen melalui proses white trap. Hasil analisis uji viabilitas antara dua isolat lokal berkecenderungan meningkat sejalan dengan naiknya level pH. Viabilitas tertinggi ditemukan pada suspensi dengan pH 8, kemudian pada pH 8,5 dan pH 9 cenderung menurun. Viabilitas terendah ditemukan pada pH 5. Pada pH 8 viabilitas NEP isolat Semarang mencapai 91.50% dan isolat Jember 97.14%. Hasil uji mortalitas terlihat bahwa mortalitas rayap tanah berkecenderungan meningkat sejalan dengan naiknya dosis yang diberikan. Pengaruh dosis NEP terhadap mortalitas rayap tanah level konsentrasi yang efektif pada pengamatan 24 jam adalah dosis 250 JI/ml. Terdapat pengaruh pH media terhadap viabilitas serta pH optimum antara isolat Semarang dan isolat Jember sama yaitu pH 8 yang mampu mempertahankan viabilitas NEP tertinggi. Isolat lokal Semarang dan Jember yang efektif berpengaruh terhadap mortalitas rayap tanah yaitu pada dosis NEP 250 JI/ml. Perlu dilakukan uji lanjut dosis yang efektif pada skala lapang untuk mendapatkan dosis yang tepat. Entomopathogenic nematodes (NEP) is a microorganism 700-1200 micron-sized worm-shaped and in the soil. NEP local isolates have different ecological character in each region. pH significant effect on mortality NEP. The high use of synthetic pesticides can lead to bad for the environment. NEP biological control agents have a high pathogenicity value against subterranean termites. The purpose of this study was to determine the optimum pH media on the viability of NEP two local isolate and determine the effect of dose on mortality of termites for 24 hours. The sample used was NEP Semarang isolates and isolates Jember. Isolation NEP use hongkong caterpillars then harvested through the white trap. The results of the analysis of the viability test between two local isolates tended to rise in line with increasing pH level. The highest viability was found in suspension with a pH of 8, then at pH 8.5 and pH 9 tends to decrease. Lowest viability was found at pH 5. At pH 8 isolates Semarang viability NEP reached 91.50% and 97.14% of isolates Jember. The test results shows that the mortality of termites land mortality tended to increase in line with the increase in the dose administered. Effect of dose on mortality NEP subterranean termites effective concentration level at 24 hours observation is a dose of 250 JI / ml. There is the influence of the media on the viability and the pH optimum pH between Semarang isolates and isolates of the same Jember is pH 8 were able to maintain the viability of the highest NEP. Jember local isolate Semarang and effective influence on the mortality of subterranean termites which the NEP dose of 250 JI / ml. Necessary to test further the effective dose on a scale field to get the proper dose.
Phytochemical Analysis Of Babadotan Leaves Extract In Flour Beet Control Solechatun, Solechatun; Widiyaningrum, Priyantini
Life Science Vol 9 No 1 (2020): April 2020
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v9i1.47143

Abstract

Tribolium castaneum is one of the warehouse insects that attack post-harvest products. More flour beetles are found in processed post-harvest products such as wheat flour and rice flour. this is because wheat flour has a higher protein content. This study aims to determine the effect of Ageratum conyzoides leaf extract in controlling flour beetles. The design of this study is a complete one-way random design. This study uses 5 different concentrations. Namely 0%, 25%, 50%, 75% and 100%. Babadotan leaf extract was macerated using 96% ethanol. There are 10 flour beetles. To determine the effect of babadotan leaf extract, this observation was carried out using a Y-olfactometer. In addition to knowing what anti-insecta compounds contained in babadotan leaf extract, the extract was analyzed by phytochemical analysis by GC-MS method. The results of the analysis of the effects of babdotan leaf extract were analyzed by Anova one-way statistics and advanced LSD test. One-way Anova results showed a difference in the effect of the concentration of babadotan leaf extract. increasing the amount of concentration shows different effects of repellent. Based on the results of the study, the use of 100% babadotan leaf extract concentration gives a high repellent effect in controlling flour beetles. Kumbang tepung (Tribolium castaneum) adalah salah satu serangga gudang yang menyerang produk pasca panen. Kumbang tepung lebih banyak ditemukan pada produk pasca panen yang sudah diolah seperti tepung gandum dan tepung beras. hal ini dikarenakan tepung gandum memiliki kandungan protein yang lebih tinggi. penelitian ini bertujuan untuk mengetahui efek dari ekstrak daun babadotan dalam mengendalikan kumbang tepung. Rancangan penelitian ini adalah rancangan acak lengkap satu arah. Penelitian ini menggunakan 5 konsentrasi yang berbeda. Yaitu 0%;25%;50%;75% dan 100%. Esktrak daun babadotan di maserasi dengan menggunakan etanol 96%. Kumbang tepung yang digunakan sebanyak 10 ekor. Untuk mengetahui efek dari ekstrak daun babadotan maka pengamatan ini dilakukan dengan menggunakan olfaktometer bentuk Y. Selain itu untuk mengetahui senyawa anti insecta apa yang terkandung dalam ekstrak daun babadotan, maka ekstrak di analisis fitokimia dengan metode GC-MS. Hasil analisis efek ekstrak daun babdotan dianalisis statistika Anova satu arah dan uji lanjut BNT. Hasil Anova satu arah menunjukkan adanya perbedaan pengaruh konsentrasi ekstrak daun babadotan. pertambahan jumlah konsentrasi menunjukkan efek repelen yang berbeda-beda. Berdasarkan hasil penelitian, penggunaan konsentrasi ekstrak daun babadotan seesar 100% memberikan efek repelen tinggi dalam mengendalikan kumbang tepung
Kombinasi Pakan Limbah Kulit Pisang dan Kulit Ari Kedelai terhadap Bobot Larva Hermetia illucens (BSF) dan Indeks Pengurangan Sampah rida nur afifah; Dyah Rini Indriyanti; Priyantini Widiyaningrum; Ning Setiati
Life Science Vol 12 No 2 (2023): November 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i2.70837

Abstract

Hermetia illucens, often called BSF larvae, is an insect that has a high enough protein content so that it becomes an alternative animal feed that fulfills a high and quality protein source for animal feed. Bioconversion that utilizes organic materials by involving living organisms. Utilization of waste materials from banana peels and soybean husks as feed media for BSF larvae. This study aims to analyze the results of feeding a combination of banana peels and soybean epidermis on weight, waste reduction index and protein and fat content in BSF larvae. This study used an experimental method with the RAL technique (completely randomized design) with five treatments and four repetitions A : 100% banana peel waste, B : 75% banana peel + 25% soybean husk, C : 50% epidermis + 50% peel banana, D : 25% banana peel + 25% soybean epidermis, E : 100% soybean epidermis using 2kg of feed medium for each treatment. The results showed that the heaviest final weight was in treatment D (25% banana peel and 75% soybean epidermis) with an average final larval weight of 0.15 grams, the waste reduction index was 3.78%/day. The highest protein content in this study was in treatment A (100% banana peel) namely 48.97% and the highest fat content was in treatment D (25% banana peel and 75% soybean husk) namely 32.69%.
Co-Authors - Wulandari, - Abadia Delima Abdillah, M.L. Adelia Rizki Purwidya Pertami, Adelia Rizki Purwidya Aditya Marianti Afifah, Nuryatul Afifah, Nuryatul Ananda, Rahimma Artantia Andin Irsadi Andreas Priyono Budi Prasetyo Ari Yuniastuti Astuti, Rafita Puji Aulia, Ike Nur Aulia, Ike Nur Aurilia Hakim, Tri Bambang Priyono Banu Wicaksono Candrawati, Devy Dalaila, Isvana Defi Fahrul Meilina, Defi Fahrul Diennurrahma, Aufa Dyah Rini Indriyanti Elawati, Nunung Eni Eling Purwantoyo Endah Peniati Enni Suwarsi Rahayu Erlianti, Susi Erlianti, Susi Fatria Fadillah Nur, Wibowo Febriana, Ayu Kurnia Febriana, Ayu Kurnia Feri Dwi Agustina Feri Dwi Agustina Haris, Amnan Indriastuti Indriastuti, Indriastuti Karim, Maftukhatul Khasanah, Minnathul Khofilla, Tyas Komari, Muh. Krispinus Kedati Pukan Kustiani, Eri Kustiani, Eri Labibah, V.R. Lina Herlina Lisdiana Lisdiana Lisdiana, Lisdiana Lisdiana Maria Ayu Puspita Martien Herna Susanti Maulana, M. Zuhrufi Mauludah, Nurul Meidina Rahmawati Melinda, Aura Tirsa Minnathul Khasanah Muhammad Abdullah Muhammad Ian Nugraha, Muhammad Ian Muthiati, Aisyaah Na'imah, Nuning Nadrotun Nana Kariada Trimartuti Niken Subekti Ning Setiati Ning Setiati Ning Setiati Nitta Jayanti, Nitta Nugrahaningsih WH, Nugrahaningsih Nugroho Edi Kartijono Nur Cholifah NUR CHOLIFAH Nur Rahayu Utami Nur, Wibowo Faria Fadillah Nurfa Anung Anidityas, Nurfa Anung Nurul Fitria Awalliyah, Nurul Fitria Oktafiyani, Adila Partaya, Partaya Pramesti Dewi Rani, Bone Kartika rida nur afifah Rohman, Alif Khoirur Romadhoni, Mar`atush Sholihah Saiful Ridlo Saraswati, Shinta Almayra Satria, Arif Bayu Siahaan, Evi Verawati Sigit Saptono Siti Farida Siti Harnina Bintari Solechatun, Solechatun Sri Mulyani Endang Susilowati, Sri Mulyani Endang Sri Ngabekti Sri Widarti, Sri Supriyanto Supriyanto Suwarti Suwarti Tri Hastutiningsih Tuti Widianti Usman Usman Utami, Annastesia Berliyan W, Deasy Amalia Wahyu Widodo Wicaksono, Banu Widyastuti, Novi Wulandari -, Wulandari Wulandari Wulandari Yati, Ernallah Yatin Mulyono Yustinus Ulung Anggraito