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Pengaruh Rasio Crude Enzim Aspergillus niger dan Trichoderma reesei terhadap Kadar Gula dan Bioetanol Hasil Fermentasi Kulit Buah Nangka (Artocarpus heterophyllus Lamk.) Diana Pertiwi; Trianik Widyaningrum
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p18

Bioethanol is ethanol derived from biological sources and can be used as an alternative fuel. Bioethanol can be made from materials that contain cellulose, for example jackfruit rind which contains 38.69% cellulose. The purpose of this study was to determine the effect of the ratio of crude enzymes Aspergillus niger and Trichoderma reesei to the sugar and bioethanol content of fermented jackfruit rind using Zymomonas mobilis. This type of research is experimental research. The experimental design used RAL with the independent variable ratio of crude Aspergillus niger and Trichoderma reesei with variants (0:0), (0:1), (1:0), (1:1), (1:2), (2:1 ), (1:3), and (3:1). The dependent variables were sugar content and bioethanol from fermented jackfruit rind. Sugar content was measured by the DNS method. Ethanol content was measured using an alcoholmeter. Experimental data were analyzed by ANOVA. The results showed that the ratio of crude enzymes Aspergillus niger and Trichoderma reesei affected the sugar content and bioethanol fermented jackfruit peel using Zymomonas mobilis. The highest sugar content in the crude enzyme ratio Aspergillus niger: Trichoderma reesei 1:0 of ??11.99% and the highest ethanol content of the crude ratio of 2: 1, which is 3.16%.

Pengaruh Konsentrasi Crude Enzim Bacillus subtilis IFO 13719 terhadap Kadar Gula dan Etanol Fermentasi Kulit Coffea arabica Menggunakan Zymomonas mobilis IFO 13756 Dheti Suraningsih; Trianik Widyaningrum
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i2.6097

Fossil energy is part of the needs of people in any country, including Indonesia. The need for fossil energy is increasing day by day. Therefore, efforts were made to find alternative raw materials from the non-food sector for the manufacture of ethanol. Cellulose material has potential as an alternative raw material for the manufacture of ethanol. One of the wastes that can be used is Arabica coffee skin. This study aims to determine the effect of the concentration of crude cellulase enzyme on sugar and ethanol content of fermented Arabica coffee husk using Zymomonas mobilis and to determine the concentration of crude cellulase enzyme which produces the highest sugar and ethanol content of fermented Arabica coffee husk using Zymomonas mobilis. This research is an experimental study with the independent variables, namely the concentration of crude cellulase enzymes, namely 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% (V/V), and the dependent variable is the sugar content of hydrolysis with crude cellulase enzyme and the ethanol content of fermented with Zymomonas mobilis. The results of the experimental research were analyzed using regression test, ANOVA, and Duncan's test. The addition of crude cellulase enzyme Bacillus subtilis influence the increase in sugar and bioethanol content of fermented Arabica coffee husks using Zymomonas mobilis. The highest sugar content in the P6 treatment (concentration of crude cellulase enzyme Bacillus subtilis 15%) was 0.93 g/mL. The highest levels of bioethanol in treatment P4 (crude cellulase enzyme Bacillus subtilis concentration 10%) was 4.46%.

Produksi Bioetanol Limbah Nasi Aking Fermentasi Menggunakan Zymomonas mobilis dengan Perlakuan Konsentrasi Crude Enzim Bacillus amyloliquefaciens Sekar Widyastanti; Trianik Widyaningrum
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i2.6249

Bioethanol fuel has the advantage of being more environmentally friendly than petroleum. Bioethanol is made from organic materials containing glucose. Baking rice contains 83.19% (w/w) carbohydrates and 29.70% (w/w) amylose which can be converted into bioethanol through hydrolysis and fermentation stages. This type of research is an experimental research, the initial stage carried out is the hydrolysis stage to obtain reducing sugars using crude Bacillus amyloliquefaciens enzymes with concentrations of 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15 %, and 17.5% for 6 days. The next step is the fermentation stage to convert reducing sugar into ethanol using Zymomonas mobilis at a concentration of 10% for 4 days. And the final result is the distillation stage and the calculation of bioethanol levels using an alcohol meter. During the hydrolysis and fermentation processes, the sugar content was measured using the DNS (Dinitrosalicylic acid) method and the highest average sugar content was obtained at 10% Bacillus amyloliquefaciens concentration, namely 1.417 g/mL. The highest bioethanol content obtained was 3.03% at 10% Bacillus amyloliquefaciens concentration and the lowest bioethanol content was 0.61% at 0% Bacillus amyloliquefaciens concentration.

Pengaruh Konsentrasi Crude Enzim Bacillus subtilis terhadap Kadar Gula dan Bioetanol Hasil Fermentasi Kulit Singkong Menggunakan Zymomonas mobilis Arief Abdillah Nurusman; Trianik Widyaningrum; Rifda Khairun Nisa; Listiatie Budi Utami
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9491

Bioethanol is an environmentally friendly and renewable alternative fuel. The use of alternative fuels is necessary because petroleum fuels make a major contribution to increasing greenhouse gas concentrations, especially CO2. Bioethanol can be obtained from materials containing carbohydrates such as cassava peel which has a high carbohydrate content, namely 4.55%. The aim of this research was to see the effect of the crude enzyme concentration of Bacillus subtilis on the results of sugar and bioethanol content of cassava peel fermentation with Zymomonas mobilis and to find out the crude enzyme concentration that had the greatest influence. This research is an experimental study with the independent variable, namely the concentration of crude cellulase enzyme B. subtilis with concentrations of 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% , and the dependent variables are sugar content from hydrolysis and bioethanol content from the fermentation process. The data obtained were analyzed using the ANOVA test and Duncan's test. The results of research on the effect of crude Bacillus subtilis cellulase enzyme concentrations of 8 concentrations have an influence on the amount of sugar and bioethanol produced from fermentation of cassava peel with Zymomonas mobilis. Crude Bacillus subtilis cellulase enzyme with a concentration of 17.5% had the greatest influence on sugar and bioethanol levels with a sugar content of 0.37% and a bioethanol content of 1.67%.

Pengaruh Rasio Crude Enzim Selulase Trichoderma reesei dan Aspergillus niger terhadap Kadar Gula dan Bioetanol Fermentasi Kulit Kacang Tanah (Arachis hypogaea L.) Menggunakan Zymomonas mobilis Trianik Widyaningrum; A’isyah Arroobi’atu Rizqiyah
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9487

The need for fuel oil in Indonesia is increasing along with the increase in the number of people's consumption in the fields of transportation, industry, and economy. While the source of petroleum fuel in Indonesia is running low. Therefore, it is necessary to utilize alternative energy, namely bioethanol. Bioethanol can be produced from organic waste that contains high cellulose, one of which is peanut shells. This study aims to determine the effect of the ratio of crude cellulase enzyme Trichoderma reesei and Aspergillus niger on sugar and ethanol content of peanut shell fermentation using Zymomonas mobilis, and to determine the ratio of crude cellulase enzyme that produces the highest sugar and ethanol content of peanut shell fermentation using Z. mobilis. This research is an experimental study with independent variables, namely the ratio of crude cellulase enzyme T. reesei : A. niger (0:0), (1:0), (0:1), (1:1), (1:2), (2:1), (1:3), and (3:1) and the dependent variable of sugar and biethanol content. Data analysis of experimental research results using ANOVA and DMRT test. The addition of crude cellulase enzyme from T. reesei and A. niger affects the sugar content and bioethanol from peanut shell fermentation. The ratio of crude cellulase enzyme from T. reesei and A. niger that produces the highest sugar content is in the treatment P5 ratio of crude enzyme T. reesei: A. niger (2:1) which is 0.14 g/mL and the highest ethanol content from groundnut shell fermentation is in the treatment of P8 crude enzyme ratio of T. reesei : A. niger (1:3) which is 2.23%.

Preparation of student activity sheets results of research on the effect of crude bacillus subtilis enzyme concentration on sugar and ethanol content from sugarcane bagasse fermentation using zymomonas mobilis as biology teaching materials Widyaningrum, Trianik; Pertiwi, Hana Eka; Utami, Listiatie Budi
JURNAL BIOEDUKATIKA Vol. 12 No. 3 (2024)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/bioedukatika.v12i3.30697

Student Activity Sheets (SAS) are teaching materials that are systematically and interestingly arranged in the learning process. In this case, the results of the study are used as material for compiling SAS. The study aims to determine the potential of SAS teaching materials from the results of the study on the effect of crude Bacillus subtilis enzyme concentration on sugar and ethanol content from bagasse fermentation using Zymomonas mobilis , the effect of crude Bacillus subtilis enzyme concentration on sugar and ethanol content from bagasse fermentation using Zymomonas mobilis , and the concentration of crude Bacillus subtilis enzyme that has the most influence on sugar and ethanol content from bagasse fermentation using Zymomonas mobilis . The research was conducted in 2 stages, the first was an experimental study conducted using a Completely Randomized Design (CRD) with the independent variable being the concentration of crude Bacillus subtilis enzyme, namely 0; 2.5; 5; 7.5; 10; 12.5; 15; 17.5% and the dependent variable being the sugar and ethanol content of the fermented sugarcane bagasse using Zymomonas mobilis . Measurement of sugar content used the DNS method, while measurement of ethanol content used an alcohol meter. Second, the preparation of SAS used the ADDIE development model which was modified to ADDE. The data from the experimental research results were analyzed using the One Way ANOVA test, while the preparation of SAS was analyzed using quantitative descriptions.. The results of the study showed that the SAS results of the study on the sugar and ethanol content of the results of the study of sugarcane bagasse using Zymomonas mobilis were categorized as very good to be used as teaching materials for biotechnology materials. The concentration of crude Bacillus subtilis enzyme that had the most influence on the sugar and ethanol content of the fermentation results of sugarcane bagasse using Zymomonas mobilis was in treatment 4 with a concentration of 10%, namely 0.67 g/mL and the highest ethanol content, namely 1.89%.

Analysis of Biology Learning Difficulties in Virus Material for Grade X Students at Muhammadiyah 3 Yogyakarta Widyaningrum, Trianik; Anggaraini , Desi
JURNAL BIOEDUKATIKA Vol. 11 No. 3 (2023)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/bioedukatika.v11i3.30912

Learning is a basic process of human development. By learning, humans make changes so that their behavior develops. Learning is not just an experience, but learning is a process and not a result. In learning activities at school, educators are faced with a number of very diverse student characteristics. The learning difficulties found at SMA Muhammadiyah 3 Yogyakarta were found to be the average class score in biology subjects was still below the KKM. The purpose of this study was to determine the internal and external factors that cause students to experience learning difficulties and to determine the level of difficulty in learning biology in understanding virus material. This type of research is descriptive quantitative. The population used in this study was 152 with a sample of 79 students. This research was conducted on June 6-7, 2024. This study used a cluster sampling technique because the data sources used were very broad. Data collection techniques used questionnaires and interviews. Data collection instruments used questionnaires and interview guidelines. The data analysis technique for this study used descriptive quantitative analysis.The results of the study showed that the internal factors that caused students in class X (E2, E4 and E4) to have difficulty learning biology on the virus material were physiological factors in the moderate category, with a percentage of 48%, 49.11% and 50%. External factors that caused students in class X-E2 to have difficulty learning biology on the virus material were school factors with a percentage of 74% in the high category, for class X-E4 were school factors with a percentage of 75% in the high category and for class X-E5 were family factors with a percentage of 76.78% in the high category. The biology sub-materials that were considered difficult on viruses were types of viruses and the life cycle of viruses

Android-based STEM-AR and its contribution to elementary school students' environmental literacy Nasaruddin, Datuk Muhammad; Maryani, Ika; Sanjun, Cindy Joe; Sulisworo, Dwi; Widyaningrum, Trianik; Santosa, Achadi Budi
International Journal of Education and Learning Vol 7, No 1 (2025): April
Publisher : Association for Scientific Computing Electrical and Engineering(ASCEE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31763/ijele.v7i1.1818

This study is motivated by the low environmental literacy and academic achievement of elementary school students. The study aims to measure the effect of Android-based STEM-AR on students' environmental literacy. A quantitative research method with a One Group Pretest-Posttest Design was used. A total of 31 fifth-grade elementary school students in Yogyakarta, Indonesia, were randomly selected from nine public schools. The research instruments included a lesson plan and an Augmented Reality (AR) medium accessible via Android smartphones. STEM was used as a reference for structuring subject matter. The treatment was conducted four times, each lasting 70 minutes. Students' environmental literacy was measured before and after the treatment using a validated multiple-choice test. Data analysis employed a t-test with a 5% significance level. The Paired Sample Test results showed a sig(2-tailed) value of 0.001 0.05, which led to the rejection of the null hypothesis and acceptance of the alternative hypothesis. This study contributes to proving that Android-based STEM-AR positively influences elementary school students' environmental literacy.

Pengaruh Rasio Crude Enzim Selulase Trichoderma reesei dan Aspergillus niger terhadap Kadar Gula dan Bioetanol Fermentasi Kulit Kacang Tanah (Arachis hypogaea L.) Menggunakan Zymomonas mobilis Widyaningrum, Trianik; Rizqiyah, Aâ€™isyah Arroobiâ€™atu
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9487

The need for fuel oil in Indonesia is increasing along with the increase in the number of people's consumption in the fields of transportation, industry, and economy. While the source of petroleum fuel in Indonesia is running low. Therefore, it is necessary to utilize alternative energy, namely bioethanol. Bioethanol can be produced from organic waste that contains high cellulose, one of which is peanut shells. This study aims to determine the effect of the ratio of crude cellulase enzyme Trichoderma reesei and Aspergillus niger on sugar and ethanol content of peanut shell fermentation using Zymomonas mobilis, and to determine the ratio of crude cellulase enzyme that produces the highest sugar and ethanol content of peanut shell fermentation using Z. mobilis. This research is an experimental study with independent variables, namely the ratio of crude cellulase enzyme T. reesei : A. niger (0:0), (1:0), (0:1), (1:1), (1:2), (2:1), (1:3), and (3:1) and the dependent variable of sugar and biethanol content. Data analysis of experimental research results using ANOVA and DMRT test. The addition of crude cellulase enzyme from T. reesei and A. niger affects the sugar content and bioethanol from peanut shell fermentation. The ratio of crude cellulase enzyme from T. reesei and A. niger that produces the highest sugar content is in the treatment P5 ratio of crude enzyme T. reesei: A. niger (2:1) which is 0.14 g/mL and the highest ethanol content from groundnut shell fermentation is in the treatment of P8 crude enzyme ratio of T. reesei : A. niger (1:3) which is 2.23%.

Pengaruh Konsentrasi Crude Enzim Bacillus subtilis terhadap Kadar Gula dan Bioetanol Hasil Fermentasi Kulit Singkong Menggunakan Zymomonas mobilis Nurusman, Arief Abdillah; Widyaningrum, Trianik; Nisa, Rifda Khairun; Utami, Listiatie Budi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9491

Bioethanol is an environmentally friendly and renewable alternative fuel. The use of alternative fuels is necessary because petroleum fuels make a major contribution to increasing greenhouse gas concentrations, especially CO2. Bioethanol can be obtained from materials containing carbohydrates such as cassava peel which has a high carbohydrate content, namely 4.55%. The aim of this research was to see the effect of the crude enzyme concentration of Bacillus subtilis on the results of sugar and bioethanol content of cassava peel fermentation with Zymomonas mobilis and to find out the crude enzyme concentration that had the greatest influence. This research is an experimental study with the independent variable, namely the concentration of crude cellulase enzyme B. subtilis with concentrations of 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% , and the dependent variables are sugar content from hydrolysis and bioethanol content from the fermentation process. The data obtained were analyzed using the ANOVA test and Duncan's test. The results of research on the effect of crude Bacillus subtilis cellulase enzyme concentrations of 8 concentrations have an influence on the amount of sugar and bioethanol produced from fermentation of cassava peel with Zymomonas mobilis. Crude Bacillus subtilis cellulase enzyme with a concentration of 17.5% had the greatest influence on sugar and bioethanol levels with a sugar content of 0.37% and a bioethanol content of 1.67%.

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