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All Journal HAYATI Journal of Biosciences Bioeksperimen: Jurnal Penelitian Biologi Jurnal Ilmu Pangan dan Hasil Pertanian Jurnal Surya Masyarakat Advance Sustainable Science, Engineering and Technology (ASSET) Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Advance Sustainable Science, Engineering and Technology (ASSET)
Dyah Ayu Widyastuti
Program Studi Biologi Fakultas Biologi UGM Yogyakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Public Health Other

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Comparison of Antioxidant Activity of Ethanolic, Methanolic, n-Hexan, and Aqueous Extract of Parkia speciosa Peel based on Half -Maximal Inhibitory Concentration Through Free Radical Inhibition Nurdyansyah, Fafa; Widyastuti, Dyah Ayu
Advance Sustainable Science, Engineering and Technology Vol 2, No 2 (2020): May-October
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v2i2.7129

Abstract

The objectives of this study was to determine the half maximum inhibitory concentration (IC50) from four types of Parkia speciosa peel extracts (ethanol, methanol, n-hexane, and aqueous)  through DPPH free radical inhibition. First Parkia’s peel extract made by drying the Parkia’s peel that has been sorted, then crushed and mashed with a blender. Parkia’s powder then macerated for 3 replication using each type of solvent and then solvent evaporation was carried out using a rotary vacuum evaporator. The evaporated extract produced then tested for antioxidant activity using the IC50 method and phytochemical screening was performed to analyze the potential content of functional compounds. The results showed that all types of solvents dissolve alkaloid compounds (except water extract), flavonoids, saponins, tannins, and phenols. IC50 values produced from the four types of petai bark extract using methanol, ethanol, water, and n-hexane solvents sequentially were 76.92; 111; 136; and 201 ppm. Methanol extract had the lowest IC50 value of 76.92 ppm which resulted that the methanol extract of petai skin had a strong (active) antioxidant strength compared to others.Keywords: Antioxidant, Exctract, Parkia’s peel, IC50
Altitude-dependent Variation in Antibacterial Properties of Red Ginger (Zingiber officinale var. rubrum): Implications for Natural Anti-Salmonella Agents Widyastuti, Dyah Ayu; Nuringtyas, Tri Rini; Rohman, Abdul; Santosa, Djoko
Advance Sustainable Science Engineering and Technology Vol. 8 No. 2 (2026): February-April
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v8i2.2781

Abstract

Red ginger contains diverse bioactive compounds with strong antioxidant and antibacterial activities. This study investigated the influence of growth location on the anti-Salmonella activity of red ginger extracts from seven regions in Java, Indonesia. The fractions were analyzed for total phenolic and flavonoid contents, as well as antioxidant capacity using DPPH and FRAP assays. Antibacterial activity was assessed against Salmonella using the Kirby-Bauer disk diffusion method. Results showed that methanol and ethyl acetate fractions exhibited the highest phenolic and flavonoid contents, while the chloroform fraction demonstrated the strongest radical scavenging activity. Extracts from Bumiaji and Lendah displayed the most potent anti-Salmonella activity (inhibition zone: 10.08 to 18.00 mm). These findings highlight that altitude and solvent polarity influence red ginger bioactivity, supporting its potential as a natural antibacterial source.
Co-Authors Abdul Rohman Andita Ayu Mandasari, Andita Ayu Dewi, Endah Rita Sulistyo Djoko Santosa fafa nurdyansyah, fafa Firdausi, Chilyatul Herawati, Dheasy Ipah Budi Minarti Kurnia, Muhammad Hardi Lussana Rossita Dewi Luthfiyah, Firya Maryam, Siti Ayu Maulana, Muhammad Iqbal Nurafifah, Diah Ayu Praptining Rahayu Tri Rini Nuringtyas