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All Journal Jurnal Ilmu Pertanian Indonesia Buletin Peternakan BIOTROPIA - The Southeast Asian Journal of Tropical Biology ANNALES BOGORIENSES Annales Bogorienses
Yantyati Widyastuti
Pusat Penelitian Bioteknologi-LIPI, Jl. Raya Bogor KM.46 Cibinong 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Veterinary Other

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The Effect of Honey on Bacterial Growth, Protein Degradation, Amino Acids Contents, and Volatile Compounds of Milks at Storage Khusniati, Tatik; Widyastuti, Yantyati
Annales Bogorienses Vol. 10 No. 1 (2005): Annales Bogorienses
Publisher : BRIN

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Abstract

Pasteurized milks spoiled at refrigerated storage due to growth of psychrotrophic bacteria. Honey which contain antibacterial and aromatic compounds may be used as supplement to inhibit psychrotrophic bacterial activities. To know nutritional and flavor compounds of milks with and without honey, effect of honey on bacterial growth protein degradation, amino acids, and volatile compounds of stored milk were detected. Bacterial growth, protein degradation, amino acids, and favor compounds were detected by total plate counts. formol titration, HPLC, and GCMS, respectively. The results how that bacterial growth and protein degradations in honey milks were lower than that without honey. Bacterial growth (5.2x103 -9.3x106 cfu/mL) and protein degradation (2.37-2.59%) in honey whole milks were lower than that (6.2x104-6.5x107 cfu/mL) (2.54-2.88%) in skim milks, respectively P<0.05). At 10 days after use by date, changing between amino acids contents in whole milks with and without honey were more significant than that or skim milk (P<0.05); and volatile compounds percentages in honey whole milks were higher than that without honey. While that in honey skim milks vice versa. Honey caused decreasing bacterial growth and protein degradation, changing amloo a id 'contents and producing volatile compounds of stored milk, and honey whole milk were better than honey skim milks.
Ecological Study of Rare-Actinomycetes in Soils and Leaf-Litters Lisdiyanti, Puspita; Ratnakomala, Shanti; Ridwan, Roni; Widyastuti, Yantyati; Otoguro, Misa; Ando, Katsuhiko
Annales Bogorienses Vol. 15 No. 2 (2011): Annales Bogorienses
Publisher : BRIN

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A total of 268 strains of Actinomycetes were isolated from soil and leaf-litter samples collected in Jambi (Sumatera) and Cibinong (West Java) using three selective isolation methods. All the isolates were identified by morphological characteristic and by analysis of 16S rDNA sequence. On the basis of their morphology and 16S rDNA sequence, 164 isolates were belonged to the Streptomyces Group and 104 isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Furthermore, 40% of isolates in Streptomyces Group and 62% of isolates in Non-Streptomyces Group are supposed to be new taxa. It is indicated the richness of Actinomycetes in these two area. The genus Streptomyces is the most abundant in soil samples, occupying 60-75% of all isolates; while the genus Actinoplanes is mainly found in leaf-litter samples (70%). The use of selective isolation media is important to elucidate the microbial diversity.
Diversity of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Lisdiyanti, Puspita; Tamura, Tomohiko; Ratnakomala, Shanti; Ridwan, Roni; Kartina, Gina; Lestari, Yulin; Ando, Katsuhiko; Widyastuti, Yantyati
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
Publisher : BRIN

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A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia using SDS-YE (SY) and Rehydration and Centrifugation (RC) isolation methods and Humic Acid Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus Streptomyces is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genus Actinoplanes is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species.
Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Susilaningsih, Dwi; Okazaki, Fumiyoshi; Yopi, Yopi; Widyastuti, Yantyati; Harayama, Shigeaki
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
Publisher : BRIN

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Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth).
Role of Lactobacillus helveticus on Flavor Formation in Cheese: Amino Acid Metabolism Widyastuti, Yantyati; Lisdiyanti, Puspita; Tisnadjaja, Djadjat
Annales Bogorienses Vol. 18 No. 1 (2014): Annales Bogorienses
Publisher : BRIN

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Abstract

Lactic acid bacteria, mainly lactobacilli, play an important role in cheese making. Their role can be divided into starters and non-starters or secondary microorganisms. Lactobacillus helveticus, an obligately homofermenter and thermophilic bacterium, has unique properties as a starter because of its ability to induce strong impact on cheese flavor. The bacteria are known to be prototrophic for 5 amino acids and auxotrophic for 13 amino acids. It is interesting that the conversion of aromatic amino acids, branch chain amino acids, and methionine into volatile and nonvolatile compounds by L. helveticus is thought to represent the rate-limiting step in the formation of mature flavor and aroma in cheese. The addition of a highly autolytic L. helveticus to a starter system could significantly increase the formation of flavor precursor and some volatile compounds during cheese ripening. This article focuses on the contribution of L. helveticus to flavour compound formation in cheese with particular emphasis on amino acid metabolism.
THE PRESERVATION OF MILK WITH THE ADDITION OF ANTIBACTERIAL AND AROMATIC SUPPLEMENTS PRODUCED IN INDONESIA Khusniati, Tatik; Widyastuti, Yantyati
BIOTROPIA Vol. 15 No. 1 (2008): BIOTROPIA Vol. 15 No. 1 June 2008
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2008.15.1.5

Abstract

The preservation of milk with additional antibacterial and aromatic supplements, produced in Indonesia, was investigated. Organoleptic performances of milk with the addition of 10% supplements, made as juices, were tested by panellists, and the total bacteria, protease activities, lipase activities and acidities, were detected by total plate counts, azocasein method, modified dole extraction and base-acid titration, respectively. Out of the 27 supplemented skim and whole milk samples, 15 whole milk samples and 10 skim milk samples were selected as acceptable, based on their better organoleptic performances, their lower bacterial counts, protease and lipase activi­ties, and their acidities percentages which were not significantly different, compared to that of control, at 5 days after the expiry date (P
Diversity of Actinomycetes From Eka Karya Botanical Garden, Bali Ratnakomala, Shanti; Lisdiyanti, Puspita; Prayitno, Nita Rosalinda; Triana, Evi; Lestari, Yulin; Hastuti, Ratih Dwi; Widyastuti, Yantyati; Otoguro, Misa; Ando, Katsuhiko; Sukara, Endang
BIOTROPIA Vol. 23 No. 1 (2016): BIOTROPIA Vol. 23 No. 1 June 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.4 KB) | DOI: 10.11598/btb.2016.23.1.504

Abstract

A total of 29 actinomycetes strains were isolated and identified by full sequence analysis of the 16S rRNA gene. Samples were collected from Bali Island, Indonesia, including 18 soil and 20 leaf litter samples from Eka Karya Botanical Garden. Two isolation methods were used in this study: SDS-extract (SY) and Rehydration-Centrifugation (RC). Based on 16S rRNA gene analysis, the isolated actinomycetes were grouped into two genera. Molecular analysis of 16S rRNA gene similarities showed that the actinomycetes isolated from Eka Karya Botanical Garden were of diverse origin. Analysis of soil samples resulted in 144 isolates belonging to 24 genera and more than 87 species, with Streptomyces being the dominant genus. This was followed by Actinoplanes (25 isolates, 17%). From leaf litter samples, 85 isolates were obtained, belonging to 9 genera and more than 41 species. The most dominant genus was Catenuloplanes (42 isolates, 49%), followed by Actinoplanes (16 isolates, 19%).
Co-Authors ANDO KATSUHIKO Anja Meryandini Azizah, Malikah Azzahra, Fikri Fatimah DWI SUSILANINGSIH Endang Sukara Evi Triana Fidriyanto, Rusli GINA KARTINA Harayama, Shigeaki Harayama, Shigeaki Hastuti, Ratih Dwi KATSUHIKO ANDO Khusniati, Tatik Khusniati, Tatik MISA OTOGURO NITA ROSALINDA PRAYITNO Nur Rochmah Kumalasari Nurul Fitri Sar Okazaki, Fumiyoshi Okazaki, Fumiyoshi PUSPITA LISDIYANTI ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN SHANTI RATNAKOMALA Tisnadjaja, Djadjat Tisnadjaja, Djadjat TOMOHIKO TAMURA Watman, Muh Wulansih Dwi Astuti YOPI YOPI YULIN LESTARI