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All Journal Buletin Peternakan ANNALES BOGORIENSES Livestock and Animal Research Jurnal Ilmiah Ilmu-Ilmu Peternakan Annales Bogorienses
Yantyati Widyastuti
Pusat Penelitian Bioteknologi-LIPI, Jl. Raya Bogor KM.46 Cibinong 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Ecological Study of Rare-Actinomycetes in Soils and Leaf-Litters Lisdiyanti, Puspita; Ratnakomala, Shanti; Ridwan, Roni; Widyastuti, Yantyati; Otoguro, Misa; Ando, Katsuhiko
Annales Bogorienses Vol. 15 No. 2 (2011): Annales Bogorienses
Publisher : BRIN

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Abstract

A total of 268 strains of Actinomycetes were isolated from soil and leaf-litter samples collected in Jambi (Sumatera) and Cibinong (West Java) using three selective isolation methods. All the isolates were identified by morphological characteristic and by analysis of 16S rDNA sequence. On the basis of their morphology and 16S rDNA sequence, 164 isolates were belonged to the Streptomyces Group and 104 isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Furthermore, 40% of isolates in Streptomyces Group and 62% of isolates in Non-Streptomyces Group are supposed to be new taxa. It is indicated the richness of Actinomycetes in these two area. The genus Streptomyces is the most abundant in soil samples, occupying 60-75% of all isolates; while the genus Actinoplanes is mainly found in leaf-litter samples (70%). The use of selective isolation media is important to elucidate the microbial diversity.
Diversity of Actinomycetes from Soil Samples Collected from Lombok Island, Indonesia Lisdiyanti, Puspita; Tamura, Tomohiko; Ratnakomala, Shanti; Ridwan, Roni; Kartina, Gina; Lestari, Yulin; Ando, Katsuhiko; Widyastuti, Yantyati
Annales Bogorienses Vol. 16 No. 1 (2012): Annales Bogorienses
Publisher : BRIN

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A total of 137 strains of Actinomycetes were isolated from 14 soil samples collected in 3 part of Lombok Island, Indonesia using SDS-YE (SY) and Rehydration and Centrifugation (RC) isolation methods and Humic Acid Vitamin (HV) agar as isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. On the basis of their morphology and 16S rRNA gene sequence, 67% of isolates were belonged to the Streptomyces Group and 33% of isolates were belonged to the Rare-Actinomycetes (Non-Streptomyces) Group. Nine families and 15 genera were found from the samples. It is indicated the richness of actinomycetes in these area. The genus Streptomyces is the most abundant in all soil samples, occupying 67% of all isolates by using SY method; while the genus Actinoplanes is mainly found by using RC as isolation method. Two strains belong to the genus Virgisporangium and Catenulispora that showed taxonomic interest need further study for describing as ne species.
Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Susilaningsih, Dwi; Okazaki, Fumiyoshi; Yopi, Yopi; Widyastuti, Yantyati; Harayama, Shigeaki
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
Publisher : BRIN

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Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth).
Role of Lactobacillus helveticus on Flavor Formation in Cheese: Amino Acid Metabolism Widyastuti, Yantyati; Lisdiyanti, Puspita; Tisnadjaja, Djadjat
Annales Bogorienses Vol. 18 No. 1 (2014): Annales Bogorienses
Publisher : BRIN

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Lactic acid bacteria, mainly lactobacilli, play an important role in cheese making. Their role can be divided into starters and non-starters or secondary microorganisms. Lactobacillus helveticus, an obligately homofermenter and thermophilic bacterium, has unique properties as a starter because of its ability to induce strong impact on cheese flavor. The bacteria are known to be prototrophic for 5 amino acids and auxotrophic for 13 amino acids. It is interesting that the conversion of aromatic amino acids, branch chain amino acids, and methionine into volatile and nonvolatile compounds by L. helveticus is thought to represent the rate-limiting step in the formation of mature flavor and aroma in cheese. The addition of a highly autolytic L. helveticus to a starter system could significantly increase the formation of flavor precursor and some volatile compounds during cheese ripening. This article focuses on the contribution of L. helveticus to flavour compound formation in cheese with particular emphasis on amino acid metabolism.
Selection of Lactiplantibacillus plantarum strains as inoculant of rice straw fermentation and its fermentation characteristics Mernawati, Mernawati; Meryandini, Anja; Widyastuti, Yantyati
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.65024

Abstract

Objective: This study aimed to select Lactiplantibacillus plantarum strains suitable for inoculant of rice straw fermentation and to evaluate its fermentation characteristics.Methods: The experiment was conducted at the Feed Bioprocess Laboratory, Research Center for Applied Zoology, National Research and Innovation Agency (BRIN). This experiment was designed using a 3×4 factorial arrangement in a completely randomized design (CRD). The first factor was inoculant (1%), and the second was incubation time (7, 14, 28, and 58 days). The first stage was selection of 9 strains of L.  plantarum, based on the ability to lowering the pH and produce lactic acid. The second stage was observing the growth curve of the selected strains of L. plantarum as inoculant for rice straw fermentation. The third stage was monitoring of the rice straw fermentation characteristics, including pH values, concentration of lactic acid, water-soluble carbohydrates (WSC) and NH3-N, also dry matter, ash and crude protein. Data were analyzed based on analysis of variance, and if there was a significant effect, the data were further analyzed with Duncan’s Multiple Range Test.Results: The selected strains L. plantarum Ca098 and L. plantarum 1A-2 showed significant interaction result (p<0.05) compared with treating inoculant and ensiling days. The fermentation time influenced (p<0.05) the quality of rice straw fermentation including pH, concentration of lactic acid, WSC, NH3-N, and crude protein.Conclusions: L. plantarum Ca098 showed the best rice straw fermentation characteristics, with fleigh value of 163.86±5.97 at 58 days of fermentation. L. plantarum Ca098 showed the highest lactic acid, the lowest pH and improve crude protein content.
Karakteristik Endapan Cairan Rumen Sapi asal Rumah Potong Hewan sebagai Feed Supplement Budiansyah, Agus; Resmi, Resmi; Nahrowi, Nahrowi; Wiryawan, Komang G; Suhartono, Maggy T; Widyastuti, Yantyati
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 14 No 1 (2011): Mei 2011
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.854 KB) | DOI: 10.22437/jiiip.v0i0.829

Abstract

The aims of this experiment were to  identify and characterize of sediment product from cattle rumen liquor as a source of amino acids, minerals and vitamins. The sediment were obtained as pellets upon centrifugation of rumen liquor at 10,000 g for 10 minutes for collection of supernatant. The sediment were evaluated for amino acids, minerals and vitamins composition and chararacterized for the pH,  solubility of dry matter, specific density, bulk and compacted bulk densities and angle of response. Result of the experiment showed that sediment contained higher minerals:  Na, K and Fe compared with the commercial premix, but lower in B-vitamins and amino acids. The composition of mineral Na, K, and Fe from rumen liquor of local cattle was 13.47%, 7.73 % and 14.52 %,  while Na, K, and Fe from  rumen liquor of  imported cattle was 18.40%, 10.25%, and 14.52% respectively. The sediment had pH range from 10.01-10.03, the dry matter solubility  was 35.5% up to 39.1%. The sediment from imported cattle had higher specific density, bulk and compacted bulk densities and angle of response than that of local cattle. It is concluded that sediment from cattle rumen liquor contained high Na, K and Fe, low amino acids and B-vitamin, high pH and low solubility.
Co-Authors A Budiansyah ANDO KATSUHIKO Anja Meryandini Azzahra, Fikri Fatimah DWI SUSILANINGSIH Endang Sukara Fidriyanto, Rusli GINA KARTINA Harayama, Shigeaki Harayama, Shigeaki I Komang Gede Wiryawan KATSUHIKO ANDO Khusniati, Tatik Khusniati, Tatik Maggy T Suhartono Mernawati, Mernawati MISA OTOGURO Nahrowi Nahrowi Nur Rochmah Kumalasari Nurul Fitri Sar Okazaki, Fumiyoshi Okazaki, Fumiyoshi PUSPITA LISDIYANTI Resmi Resmi ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN SHANTI RATNAKOMALA Tisnadjaja, Djadjat Tisnadjaja, Djadjat TOMOHIKO TAMURA Watman, Muh Wulansih Dwi Astuti YOPI YOPI YULIN LESTARI