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All Journal Meditory : The Journal of Medical Laboratory Jurnal Peduli Masyarakat BERNAS: Jurnal Pengabdian Kepada Masyarakat Aesculapius Medical Journal Warmadewa Minesterium Medical Journal Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika Folia Medica Indonesiana
Anak Agung Ayu Lila Paramasatiari
Universitas Warmadewa
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Penerapan Protokol Kesehatan Dalam Pencegahan Penularan Covid-19 pada Kelompok Karang Taruna Dharma Santhi Anak Agung Ayu Lila Paramasatiari; Putu Arya Suryandhita; Ni Wayan Widhidewi
Warmadewa Minesterium Medical Journal Vol. 4 No. 3 (2025): September 2025
Publisher : Warmadewa University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmmj.4.3.2025.249-254

Abstract

Penularan Covid-19 masih terjadi di tengah masyarakat. Salah satu pencegahan penularan Covid-19 adalah dengan cara vaksinasi dan melakukan edukasi kepada masyarakat. Partisipasi dari berbagai kalangan diperlukan dalam pencegahan penularan penyakit infeksi ini. Berdasarkan hasil wawancara dengan pihak dusun Darma Santhi ditemukan permasalahan bahwa kalangan pemuda pemudi yang terdapat di wilayah banjar tersebut tidak pernah mendapatkan sosialisasi dari pihak sekolah maupun puskesmas mengenai protokol kesehatan secara langsung selama pandemi ini. Solusi yang ditawarkan yakni dengan melakukan edukasi berupa penyuluhan kepada siswa, pendampingan protokol kesehatan pada saat proses pembelajaran dan memberikan bantuan kepada banjar terkait protokol kesehatan yang dapat digunakan pada sekolah di wilayah banjar tersebut. Hal ini ditawarkan untuk mencegah terjadinya kluster sekolah akibat penyebaran di dalam sekolah baik dari siswa maupun guru sekolah. Kegiatan ini akan diselenggarakan secara offline dengan menerapkan protokol kesehatan yang ketat dan dilakukan di ruang terbuka dengan jumlah minimal 10 orang. Target luaran berupa peningkatan pengetahuan, tersedianya sarana dan prasarana terkait protokol kesehatan di banjar.
Identifikasi Bakteri Klebsiella Sp dan Citrobacter Sp Hasil Isolasi dari Makanan dan Minuman di Beberapa Pasar Tradisional Denpasar Paramasatiari, Anak Agung Ayu Lila; Widhidewi, Ni Wayan; Suryanditha, Putu Arya
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 7 (2024): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v7i.3542

Abstract

Makanan dan minuman merupakan sumber energi bagi manusia dalam kehidupan sehari-hari. Pemilihan makan dan minuman yang tidak cermat mengakibatkan konsumen mengonsumsi makanan dan minuman yang terkontaminasi yang dapat menimbulkan penyakit. Penyakit yang ditimbulkan melalui makanan dan minuman yang terkontaminasi disebut dengan Foodhandler diseases. Penelitian ini bertujuan untuk mengidentifikasi bakteri Klebsiella sp dan Citrobacter sp pada Makanan dan Minuman di Beberapa Pasar Tradisional. Metode Penelitian ini menggunakan rancangan deskriptif eksplorasi. Pemilihan sampel dilakukan secara acak di Pasar tradisional di wilayah Denpasar. Setiap pasar dilakukan pengambilan sampel makanan dan minuman dilakukan secara random terdiri dari 5 sampel pada masing-masing pasar yang berasal dari 7 pasar tradisional di Denpasar. Pada penelitian ini sampel makanan dan minuman diisolasi menggunakan media selective Mac Conkey dan identifikasi menggunakan uji biokimia dengan Imvic Test.Hasil ppenelitian menunjukkan pada makanan dan minuman dari 7 pasar didapatkan terkontaminasi bakteri Klebsiella sp sebanyak 2 (5,7%) dan Citrobacter sp sebanyak 8 sampel (22%). Rekomendasi yang diberikan dengan edukasi dan pemeriksaan secara rutin makanan dan minuman yang dijual untuk keamanan konsumen
GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) PROFILING AND ANTIBACTERIAL ACTIVITY OF SECONDARY METABOLITES PRODUCED BY Bacillus sp. SA11 FROM MANGROVE SOIL Ni Kadek Widya Diari Ningsih; Anak Agung Gede Indraningrat; Anak Agung Ayu Lila Paramasatiari; Daegeun Choe
Meditory : The Journal of Medical Laboratory Vol. 13 No. 2 (2025): Meditory, Volume 13 No. 2 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i2.4317

Abstract

Background: Bacterial infections are a major global health concern, including in Indonesia.  This has led to an increased effort to find new antibacterial compounds in natural resources. Bacillus sp. SA11, with reported antibacterial potential, was previously isolated from mangrove soil in Denpasar, Bali. This study further investigated its antibacterial activity.Methods: Secondary metabolites were extracted from Bacillus sp. SA11 liquid culture using ethyl acetate. The extract was then screened for antibacterial activity against Escherichia coli ATCC 25922, Streptococcus mutans FNCC 0405, Staphylococcus aureus ATCC 25923, and Klebsiella pneumoniae ATCC 70060 using the Kirby-Bauer method (three replicates). The chemical composition of the extract was analysed via GC-MS.Results: The ethyl acetate extract showed inhibition zones for E. coli, S. mutans, S. aureus, and K. pneumoniae of 11.7 ± 1.11 mm, 10.22 ± 1.5 mm, 8.7 ± 0.94 mm, and 7.2 ± 0.67 mm, respectively. GC/MS analysis identified 165 compounds, 20 of them have been  previously linked to antibacterial activities. The five most abundant compounds are n-hexadecanoic acid (6.91%), 2-butoxyethyl acetate (6.3%), Bis(2-ethylhexyl) phthalate (4.45%), Phthalic acid, di(2-propylpentyl) ester (4.45%) and Benzene, 1,3,5-trimethyl- (Mesitylene).Conclusion: These findings provide preliminary evidence that Bacillus sp. SA11, isolated from the mangrove ecosystem, has the potential to produce antibacterial compounds. 
Antibacterial activity of aloe vera in inhibiting the growth of methicillin-resistant Staphylococcus aureus Ni Putu Ratih Permata Dewi Arya; Anak Agung Ayu Lila Paramasatiari; Ni Wayan Widhidewi; Marta Setiabudy
Journal of Clinical Microbiology and Infectious Diseases Vol. 6 No. 1 (2026): Available online : 1 June 2026
Publisher : Indonesian Society for Clinical Microbiology (Perhimpunan Dokter Spesialis Mikrobiologi Klinik Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/jcmid.v6i1.112

Abstract

Introduction: Antibiotic resistance is an increasing global health problem, particularly associated with Methicillin-Resistant Staphylococcus aureus (MRSA), which shows resistance to β-lactam antibiotics and can cause severe infections. This condition has encouraged the exploration of alternative antibacterial agents derived from natural sources. Aloe vera contains various bioactive compounds, such as saponins, flavonoids, tannins, and phenolic compounds, which potentially exhibit antibacterial activity. This study aimed to determine the effectiveness of Aloe vera extract in inhibiting the growth of MRSA. Methods: This experimental laboratory study employed a posttest-only control group design using the disk diffusion method on Mueller–Hinton agar. Aloe vera extract was tested at concentrations of 15μg/mL, 20μg/mL, 25μg/mL, and 50μg/mL. Vancomycin and 0.9% NaCl were used as positive and negative controls, respectively. Antibacterial activity was evaluated by measuring the diameter of inhibition zones. Data were analyzed descriptively. Result: The findings indicated that all concentrations of Aloe vera extract failed to produce inhibition zones against MRSA, with inhibition diameters of 0.00 mm. The positive control showed strong antibacterial activity, whereas the negative control exhibited no inhibitory effect. Conclusion: Aloe vera extract at concentrations of 15%, 20%, 25%, and 50% was not effective in inhibiting the growth of Methicillin-Resistant Staphylococcus aureus (MRSA) under in vitro conditions
Co-Authors Anak Agung Istri Shita Darmajayanti Anak Agung Istri Shita Darmajayanti Anak Agung Oka Lely budiapsari, putu indah Choe, Daegeun Daegeun Choe I Gusti Ngurah Arjana I Gusti Rai Tirta I Komang Medy Wedhangga I Made Dwiky Vinandyanata I Nyoman Sutapa I Wayan Darwata Ida Ayu Putu Pradnya Dewi Ida Ayu Putu Pradnya Dewi Indraningrat, Anak Agung Gede Kapti I Nengah Made Kristya Pitaloka Gangga Dewi Made Kristya Pitaloka Gangga Dewi Maharani, Ni Luh Putu Silfia Maheswari, Ida Ayu Kartika Marta Setiabudy Ni Kadek Widya Diari Ningsih Ni Putu Aniek Mahayani, Ni Putu Aniek Ni Putu Indah Kusumadewi Riandra Ni Putu Ratih Permata Dewi Arya Ni Wayan Widhidewi Putu Arya Suryandhita Putu Arya Suryandhita Putu Arya Suryanditha Putu Sannia Dewi Putu Sannia Dewi Putu Sutisna Rika Wulandari Salsabila, Unik Hanifah Sri Masyeni, Dewa Ayu Putri Trisnia, Putu Ayunda