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All Journal Alchemy : Journal of Chemistry Buletin Palawija Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Biosaintifika: Journal of Biology & Biology Education AGROMIX Agro Bali: Agricultural Journal Buletin Palawija
Eriyanto Yusnawan
Indonesian Legumes and Tuber Crops Research Institute (ILETRI),Jalan Raya Kendalpayak KM 8, PO BOX 66, Malang East Java, Indonesia, Phone :+62341 801468, Fax: +62341 801496,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Materials Science & Nanotechnology

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Pengaruh perlakuan refugia dan jarak tanam kedelai terhadap keragaman musuh alami : The effect of refugia treatment and soybean plant spacing on natural enemy diversity Uge, Emerensiana; Sari, Kurnia Paramita; Yusnawan, Eriyanto; Inayati, Alfi
AGROMIX Vol 14 No 2 (2023)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v14i2.3576

Abstract

Introduction: Soybean cultivation is inseparable from the attack of plant pests. Control of soybean pests, which generally use synthetic pesticides, negatively impacts food products, environmental health, and ecosystem sustainability. Planting flowering plants around bunds can be useful in increasing the diversity of natural enemies because they can attract and serve as microhabitats for natural enemies. This research to obtain the effect of refugia treatment and soybean spacing on the diversity of natural enemies in soybean plantations. Methods: The study was designed in randomized block design, consisting of refugia and non-refugia treatments and plant spacing of 40 cm x 25 cm (J1) and 50 cm x 25 cm (J2), which was repeated three times. Sampling was carried out in the vegetative and generative phases, using yellow traps and pitfalls installed in the field for 24 hours. Results: The results showed that the most trapped natural enemy families were in the refugia treatment. The total number of trapped natural enemies was highest in the vegetative and generative phases, namely in the refugia and 2 spacing treatments (R-J2). The family of Braconidae order Hymenoptera and the group of parasitoids had the highest numbers in the refugia and non-refugia treatments. The value of the Diversity Index (H') in the refugia treatment and both plant spacings in the two growth phases was moderate, while in the non-refugia treatment, the two plant spacings and both growth phases were small and medium. Generally, the evenness index (D) value for all treatments is known to be small and medium. Conclusion: Refugia treatment and plant spacing affect the number of natural enemy populations, which is expected to suppress the development of pests in soybean cultivation.
Identification of Bacterial Soft Rot in Potatoes From Batu City, Indonesia Berliana, Sonia; Abadi, Abdul Latief; Aini, Luqman Qurata; Yusnawan, Eriyanto
Agro Bali : Agricultural Journal Vol 8, No 3 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i3.2312

Abstract

Soft rot is a plant disease that can reduce both the quality and quantity of potato production, primarily caused by the Pectobacterium carotovorum. This study aims to isolate and identify the bacteria responsible for soft rot disease in potato tubers using molecular methods. Bacterial isolation was performed on a diseased potato tuber collected from Sumber Brantas Village, followed by a pathogenicity test, PCR, 16S rRNA gene sequencing, and phylogenetic analysis.  Based on macroscopic and microscopic examination, four isolates exhibiting soft rot symptoms were identified: P1, P2, K5, and K7. The 16S rRNA sequencing results and BLAST analysis showed that isolate K5 was closely related to Serratia rubidaea, isolate P2 was closely associated with Pectobacterium carotovorum, isolate P1 was closely related to Pseudomonas koreensis, and isolate K7 was closely associated with Ralstonia pseudosolanacearum. This study presents the first molecular characterization of bacteria responsible for soft rot in potato tubers originating from Batu City, East Java. The findings provide foundational insights that may support the development of molecular-based diagnostic tools and region-specific disease management strategies for potato cultivation in Indonesia.
In Vitro Assessment of Endophytic Bacteria Originating from Cycas rumphii and Dioon spinulosum as Stress-Tolerant Plant Growth-Promoting Bacteria Al Husni, Muhammad Fariq; Trianti, Irisa; Yusnawan, Eriyanto; Pamungkas, Bayu Aji; Putri, Sastia Prama; Aini, Luqman Qurata
AGRIVITA Journal of Agricultural Science Vol 48, No 1 (2026)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v48i1.5072

Abstract

The ability of cycads to grow in harsh environments is thought to be associated with endophytic bacteria in their roots. This study aimed to characterize endophytic bacteria from Cycas rumphii and Dioon spinulosum as stress-tolerant plant growth-promoting bacteria (PGPB). Fine roots of C. rumphii and D. spinulosum were isolated using serial dilution plating and characterized through colony morphological observation, hypersensitivity, antagonistic activity, PGP activity, and abiotic stress tolerance assays. Fourteen isolates were obtained (eight from C. rumphii and six from D. spinulosum), with higher abundance and diversity in D. spinulosum (2.51 × 10¹⁰ CFU/g; H′ = 2.016) than C. rumphii (4.64 × 10⁹ CFU/g; H′ = 1.752). All isolates were non-pathogenic; 92% fixed nitrogen, 92% solubilized phosphate, and one (DS1A) produced IAA (0.200 ppm). Four isolates showed antagonism against Xanthomonas campestris (0.40–0.67 cm inhibition) and tolerance to drought, salinity (5–10%), and moderate heat, with growth at pH 5–6. Molecular identification by 16S rRNA sequencing revealed that CR1A, CR1B, and DS1B belong to Stenotrophomonas maltophilia, whereas DS1A belongs to Bacillus sp. These findings highlight cycads as a source of endophytic PGPB candidates for supporting plant health, particularly when grown in the environmental stress condition areas.
Co-Authors Abdul Latief Abadi abdullah Taufiq Ahwalul Lailiyah, Ahwalul Al Husni, Muhammad Fariq Alfi Inayati Alfiyaturrohmah Alfiyaturrohmah, Alfiyaturrohmah Amanah, Amri Berliana, Sonia C.P Marquis C.P. Marquis Didik Harnowo Irisa Trianti Joko Purnomo Joko Susilo Utomo Koentjoro, Bambang Sri Luqman Qurata Aini N.A. Lee Noerwijati, Kartika Novita Nugrahaeni Nyoman Djinar Setiawina Pamungkas, Bayu Aji Putri, Sastia Prama Rachmawati Ningsih Selvi Marcellia SUTRISNO TITIK SUNDARI Tri Kustono Adi Uge, Emerensiana Uge, Emerensiana Yuliantoro Baliadi