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All Journal agriTECH Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Abdi Insani Bioscientist : Jurnal Ilmiah Biologi Journal of Science and Science Education (JoSSEd) Jurnal Warta Desa (JWD) Prosiding Konferensi Nasional PKM-CSR Jurnal Pijar MIPA Jurnal Pepadu Samota Journal of Biological Sciences (SJBIOS) Biodiversitas Journal of Biological Diversity
Sarkono Sarkono
Program Studi Biologi Fakultas MIPA Universitas Mataram, Mataram, Indonesia
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Isolation and Identification of Acid-Forming Bacteria in Arabica Coffee Fruit (Coffea arabica) During Wet Fermentation Qothrunnada Amira Salma; Sarkono Sarkono; Aida Muspiah
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.7335

Abstract

The distinctive flavor and aroma possessed by Arabica coffee are an attraction for coffee enthusiasts. Post-harvest processing of coffee such as coffee fruit fermentation also contributes to the formation of the flavor and aroma of Arabica coffee. In this study, spontaneous fermentation was conducted by adding distilled water, with treatments on fermentation days 4 and 7 aimed at detecting acid-forming bacteria that played a role in the fermentation process. The bacterial isolates were characterized through macroscopic and microscopic observations, physiological, and biochemical tests. Identification of bacterial isolates was performed using the profile matching method based on Bergey’s Manual of Systematic Bacteriology (Holt et al., 1994) and Axellson (2004) until the genus level. The identified bacterial genera were Asaia, Gluconobacter, Neoasaia, Saccharibacter, and Lactobacillus.
Fishmeal-Based Media Supports Growth and Endospore Production of Locally-Isolated Lysinnibacillus sphaericus and Induces its Toxicity to 3rd Instar Aedes aegypti Larvae in Laboratory Conditions Bambang Fajar Suryadi; Ika Mustikasari; Zuriatun Annisa; Sarkono; Galuh Tresnani
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.7361

Abstract

The aim of this study was to determine whether fishmeal-based media could be used to grow L. sphaericus and induce its toxicity against Aedes aegypti larvae. Three concentrations (10, 20 and 30%) of fishmeal-based media were used to grow L. sphaericus isolate Bs2-1-2. Cell growth and endospore production were observed every 12 hours for 72 hours. The lethal concentration was measured every 24 hours for 72 hours of fermentation. The highest cell concentration was found in L. sphaericus grown on the media with 30% fishmeal concentration (3.03x1008 cells/mL), followed by 20% concentration (2.43x1008 cells/mL) and the lowest at 10% concentration (2.20x1008 cells/mL). At the end of fermentation, the highest concentration was found in L. sphaericus grown on 30% fishmeal-based media (1.51x1008 cells/mL), followed by 20% media (6.95x1007 cells/mL) and 10% media (3.21x1007 cells/mL). After 72-hour incubation, the highest endospore concentration was achieved by L. sphaericus grown on 20% (2.51x1008 cells/mL) and 10% (2.19x1008 cells/mL) fishmeal-based media. Initial larval toxicity of L. sphaericus showed the highest mortality on 20 and 30% fishmeal-based media (both reaching 53.33%), while 10% fishmeal-based media gave only 26.67% larval mortality. The LC50 value at 72 hours was achieved by L. sphaericus cultured on 30% fishmeal-based media (2.47 x 1008 cells/mL), followed by 20% concentration (4.82 x 1008 cells/mL) and 10% concentration (9.01 x 1009 cells/mL). The conclusion of this study was all concentrations of fishmeal-based media could support cell growth, endospore production and larval toxicity induction of L. sphaericus.
Isolation of Bacillus subtilis from Several Locations in Mataram and its Toxicity to Aedes aegypti Mosquitoes in the Laboratory Alya Supriyani; Laila Annisa Rahmah; Sarkono Sarkono; Galuh Tresnani; Bambang Fajar Suryadi
Samota Journal of Biological Sciences Vol 5 No 1 (2025): Samota Journal of biological Sciences
Publisher : University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/kc6ken06

Abstract

One way to biologically control mosquitoes is by utilizing bacteria. Bacillus thuringiensis and Bacillus sphaericus are commonly used bacteria for this purpose. However, Bacillus subtilis has recently attracted the attention of researchers, as it is reported to have potential for use as a biopesticide. In this study, B. subtilis was isolated from several habitats in Mataram and West Lombok and tested on Aedes aegypti larvae to determine its potential toxicity. B. subtilis was isolated from the soil using a heating technique to separate it from other bacteria that do not have endospores and then grown at 33oC for 24 hours. The bacteria that grew were then tested on third-instar Ae. aegypti larvae. From several sampled locations, eight B. subtilis isolates with low toxicity levels were obtained. Toxic B. subtilis isolates were obtained from garden soil (isolate LT2C, 20%) and rhizosphere soil (isolate LB1A, 30%). The toxicity obtained is lower than the toxicity of B. thuringiensis and B. sphaericus, therefore further exploration is needed to be able to utilize B. subtilis as a biopesticide agent.
PELATIHAN PRODUKSI DAN APLIKASI PUPUK HAYATI MIKROBIAL UNTUK MENDUKUNG EKOPESANTREN DI PONPES SYAIKH ABDURRAHMAN, LOMBOK TIMUR Ernin Hidayati; Bambang F. Suryadi; Baiq Jelita; Fatahillah Ardhani; Almachita F. Mawatri; Windi S. Putri; Ega A. Kamula; Didik Y. Wahyu; Difaizti Alinsyirah; Ataya A. Maryadi; Puspa D. Salsabila; Anak A.A. Nara Kusuma; Sarkono; Faturrahman; Tri Mulyaningsih; Syarqul Ausath
Jurnal Abdi Insani Vol 13 No 5 (2026): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v13i5.3627

Abstract

Pondok Pesantren Syaikh Abdurrahman di Desa Kotaraja, Lombok Timur mengembangkan program ekopesantren dengan penanaman pohon gaharu (Gyrinops versteegii) di lahan pesantren. Permasalahan pertumbuhan bibit gaharu  adalah tingkat kelulushidupannya yang relatif rendah dan pertumbuhan awal yang relatif lama. Pemupukan merupakan salah satu solusi untuk meningkatkan kelulushidupan dan mempercepat pertumbuhan awal bibit gaharu. Tujuan kegiatan adalah untuk memberikan pengetahuan dan keterampilan sains praktis kepada santri berupa pembuatan pupuk hayati mikrobial dan aplikasi pemupukan pada bibit gaharu. Manfaat yang diperoleh dari kegiatan ini adalah santri memperoleh pengetahuan dan keterampilan praktis dalam membuat dan mengaplikasikan pupuk hayati mikrobial secara mandiri. Peserta dalam kegiatan ini adalah santri putra  dan santri putri berjumlah 20 orang.  Santri diberikan teori atau pemahaman mengenai pupuk dan pertumbuhan tanaman. Santri juga diajarkan dan didampingi praktek membuat pupuk organik hayati dan mengaplikasikannya pada bibit tanaman gaharu. Tingkat ketercapaian kegiatan dievaluasi secara kualitatif dan kuantitatif melalui kuesioner dan penilaian keterampilan langsung oleh tim pengabdian. Hasil menunjukkan bahwa sebelum kegiatan dilaksanakan, sebanyak 58,8 % peserta  telah memahami fungsi pupuk bagi tanaman, 47 % mengetahui contoh pupuk hayati mikrobial, 17,65 % mengetahui contoh pupuk hayati, 0% mengetahui cara membuat pupuk hayati mikrobial, serta 0% mengetahui cara mengaplikasikan pupuk tersebut pada tanaman. Setelah kegiatan, tingkat ketercapaian aspek tersebut telah 100%. Teori dan praktik yang diberikan selama kegiatan tidak hanya meningkatkan pemahaman dan keterampilan praktis pembuatan serta aplikasi pupuk hayati mikrobial dari segi jumlah peserta tapi juga kualitas pemahaman dan keterampilannya.
Identification of Potentially Pathogenic Bacteria in Fresh Marine Fish Sold at Kebon Roek Market, Mataram Baiq Kesy Juliani; Sarkono; Bambang Fajar Suryadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.19823

Abstract

This study aimed to enumerate, detect, and identify potentially pathogenic bacteria contaminating fresh marine fish sold at Kebon Roek Market, Mataram City. This preliminary laboratory-based descriptive survey focused on the fish species most frequently purchased by consumers: eastern little tuna (Euthynnus sp.), sardinella (Sardinella sp.), and Indian mackerel (Rastrelliger sp.). Fish samples were collected from nine purposively selected vendors located in different sections of the market, comprising three vendors each from the front, middle, and rear areas. Laboratory analyses included determination of the Total Plate Count (TPC), bacterial isolation using selective media, and bacterial identification based on cellular morphology, Gram-staining reactions, and biochemical characteristics. The bacterial loads were 3.36 × 10⁶ CFU/g in eastern little tuna, 1.76 × 10⁶ CFU/g in Indian mackerel, and 4.85 × 10⁵ CFU/g in sardinella. The bacterial counts detected in eastern little tuna and Indian mackerel exceeded the maximum limits specified in the Indonesian National Standard SNI 7388:2009. Bacterial identification revealed the presence of potentially pathogenic bacteria, including Enterobacter spp., Citrobacter spp., Escherichia coli, Edwardsiella spp., Vibrio spp., and Aeromonas spp. These findings indicate that fresh marine fish sold at Kebon Roek Market may serve as a vehicle for transmitting pathogenic bacteria to consumers. Therefore, improved hygiene and sanitation practices are required throughout the fish distribution and retail chain to ensure food safety.
Lactic acid bacteria isolated from the digestive tract of spiny lobsters (Panulirus homarus) and their potency as probiotics FATURRAHMAN FATURRAHMAN; KIKI RIZKI AULIYAH; LARAS ALISHIA JUANDA; TRI WAHYU SETYANINGRUM; SARKONO SARKONO; BAMBANG FAJAR SURYADI; FARIQ AZHAR; BAYU PRIYAMBODO; WANDA QORIASMADILLAH
Biodiversitas Journal of Biological Diversity Vol. 26 No. 4 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260432

Abstract

Abstract. Faturrahman, Auliyah KR, Juanda LA, Setyaningrum TW, Sarkono, Suryadi BF, Azhar F, Priyambodo B, Qoriasmadillah W. 2025. Lactic acid bacteria isolated from the digestive tract of spiny lobsters (Panulirus homarus) and their potency as probiotics. Biodiversitas 26: 1827-1835. Spiny lobsters (Panulirus homarus) are an on-demand marine fishery commodity with significant economic value. However, collected data indicate that the lobsters exhibit low growth rates and high mortality due to disease infection caused by Vibrio spp. The provision of probiotics in feed represents a potential solution to this issue. Lactic acid bacteria (LAB) have been identified as probiotics due to their ability to counteract pathogenic bacteria and synthesize enzymes capable of hydrolyzing complex molecules, such as proteins, carbohydrates, and lipids, thereby promoting organismal growth. The objective of this study was to isolate LAB from the digestive tract of spiny lobsters as probiotic candidates. The selection of LAB isolates from the digestive tract was based on their ability to inhibit the growth of Vibrio harveyi bacteria and their capacity to produce a range of extracellular enzymes, including proteases, lipases, and amylases. The results demonstrated the isolation of seven LAB isolates, classified as Pediococcus, Enterococcus, and Lactobacillus genera, which exhibited the capacity to impede the proliferation of V. harveyi. The majority of the isolated LAB were found to possess the ability to hydrolyze protein, starch, and fat, in addition to exerting inhibitory effects against the growth of V. harveyi. Among the isolates, SP6 exhibited the most promising characteristics as a probiotic candidate by demonstrating robust protein and fat hydrolysis abilities, along with a significant capacity to inhibit the growth of V. harveyi.
Evaluation of Entomopathogenic Bacteria Isolated from Bamboo Rhizosphere Soil in Mataram City and Their Toxicity Against Instar III Aedes aegypti Larvae in a Laboratory Environment Bambang Fajar Suryadi; Ernin Hidayati; Faturrahman Faturrahman; Anak Agung Ngurah Nara Kusuma; Sarkono Sarkono
Jurnal Biologi Tropis Vol. 26 No. 4 (2026): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i4.13026

Abstract

Aedes aegypti is an important vector of dengue and other mosquito-borne diseases. Entomopathogenic bacteria from the bamboo rhizosphere may provide a potential biological approach for controlling its larvae. This study aimed to isolate entomopathogenic bacteria that are able to suppress Aedes aegypti mosquito larvae in a laboratory environment. Bamboo rhizosphere soil samples were collected at several selected bamboo rhizosphere locations around Mataram City. Two hundred and fifty grams of soil was collected from a depth of 5-10 cm below the surface and placed into pre-sterilized containers. The soil in the containers was stored in a shaded area before use in the study. The bacteria were isolated by heat treatment the soil mixed with sterile distilled water (10% concentration), then conducted to serial dilution and spreading it on Nutrient Agar media supplemented with Amoxicillin and Streptomycin. The growing colonies of bacteria were then tested for their toxicity on Instar III Aedes aegypti larvae in the laboratory to determine their toxicity. Bacterial isolation from all bamboo rhizosphere locations yielded two entomopathogenic bacterial species, Bacillus thuringiensis and Bacillus sphaericus. B. thuringensis isolated from several locations exhibited toxicity to instar III A. aegypti larvae ranging from 30% to 60%, while B. sphaericus exhibited toxicity ranging from 10% to 30%. The toxicity values demonstrated by these two bacteria were categorized as low to moderate. Differences in toxicity levels between isolates confirm the influence of habitat on the bacteria's biological characteristics. This finding reinforces the role of indigenous microorganisms as an environmentally friendly and sustainable source of vector control.
Co-Authors Ahmad Jupri AIDA MUSPIAH AIDA MUSPIAH Almachita F. Mawatri Alya Supriyani Anak A.A. Nara Kusuma Anak Agung Ngurah Nara Kusuma Anak Agung Putu Sidhiawan Ataya A. Maryadi Baiq Jelita Baiq Kesy Juliani Bambang F. Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Setiaji Bambang Setiaji BAYU PRIYAMBODO Didik Y. Wahyu Difaizti Alinsyirah Ega A. Kamula Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati FARIQ AZHAR Fatahillah Ardhani Faturrahman Faturrahman Faturrahman Faturrahman FATURRAHMAN FATURRAHMAN Galuh Trenani Galuh Tresnani Galuh Tresnani Ika Mustikasari Ika Nurhimaya Immy Suci Rohyani Islamul Hadi ISLAMUL HADI Juarsahnim Juarsahnim Jurnal Pepadu KIKI RIZKI AULIYAH Laila Annisa Rahmah Langkah Sembiring Langkah Sembiring LARAS ALISHIA JUANDA Moh. Andi Setiawan Nisful Mahdi Nur Indah Julisaniah Nur Indah Julisaniah Nurul Zulfa Fitriana Puspa D. Salsabila Qothrunnada Amira Salma Rosal Okjayansah Sukarti Moeljopawiro Sukarti Moeljopawiro Sukiman . Syarqul Ausath Tri Mulyaningsih TRI WAHYU SETYANINGRUM WANDA QORIASMADILLAH Windi S. Putri Yuliadi Zamroni Zuriatun Annisa