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All Journal agriTECH Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Abdi Insani Bioscientist : Jurnal Ilmiah Biologi Journal of Science and Science Education (JoSSEd) Jurnal Warta Desa (JWD) Prosiding Konferensi Nasional PKM-CSR Jurnal Pijar MIPA Jurnal Pepadu Samota Journal of Biological Sciences (SJBIOS)
Sarkono Sarkono
Program Studi Biologi Fakultas MIPA Universitas Mataram, Mataram, Indonesia
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Isolation and Identification of Acid-Forming Bacteria in Arabica Coffee Fruit (Coffea arabica) During Wet Fermentation Qothrunnada Amira Salma; Sarkono Sarkono; Aida Muspiah
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.7335

Abstract

The distinctive flavor and aroma possessed by Arabica coffee are an attraction for coffee enthusiasts. Post-harvest processing of coffee such as coffee fruit fermentation also contributes to the formation of the flavor and aroma of Arabica coffee. In this study, spontaneous fermentation was conducted by adding distilled water, with treatments on fermentation days 4 and 7 aimed at detecting acid-forming bacteria that played a role in the fermentation process. The bacterial isolates were characterized through macroscopic and microscopic observations, physiological, and biochemical tests. Identification of bacterial isolates was performed using the profile matching method based on Bergey’s Manual of Systematic Bacteriology (Holt et al., 1994) and Axellson (2004) until the genus level. The identified bacterial genera were Asaia, Gluconobacter, Neoasaia, Saccharibacter, and Lactobacillus.
Fishmeal-Based Media Supports Growth and Endospore Production of Locally-Isolated Lysinnibacillus sphaericus and Induces its Toxicity to 3rd Instar Aedes aegypti Larvae in Laboratory Conditions Bambang Fajar Suryadi; Ika Mustikasari; Zuriatun Annisa; Sarkono; Galuh Tresnani
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.7361

Abstract

The aim of this study was to determine whether fishmeal-based media could be used to grow L. sphaericus and induce its toxicity against Aedes aegypti larvae. Three concentrations (10, 20 and 30%) of fishmeal-based media were used to grow L. sphaericus isolate Bs2-1-2. Cell growth and endospore production were observed every 12 hours for 72 hours. The lethal concentration was measured every 24 hours for 72 hours of fermentation. The highest cell concentration was found in L. sphaericus grown on the media with 30% fishmeal concentration (3.03x1008 cells/mL), followed by 20% concentration (2.43x1008 cells/mL) and the lowest at 10% concentration (2.20x1008 cells/mL). At the end of fermentation, the highest concentration was found in L. sphaericus grown on 30% fishmeal-based media (1.51x1008 cells/mL), followed by 20% media (6.95x1007 cells/mL) and 10% media (3.21x1007 cells/mL). After 72-hour incubation, the highest endospore concentration was achieved by L. sphaericus grown on 20% (2.51x1008 cells/mL) and 10% (2.19x1008 cells/mL) fishmeal-based media. Initial larval toxicity of L. sphaericus showed the highest mortality on 20 and 30% fishmeal-based media (both reaching 53.33%), while 10% fishmeal-based media gave only 26.67% larval mortality. The LC50 value at 72 hours was achieved by L. sphaericus cultured on 30% fishmeal-based media (2.47 x 1008 cells/mL), followed by 20% concentration (4.82 x 1008 cells/mL) and 10% concentration (9.01 x 1009 cells/mL). The conclusion of this study was all concentrations of fishmeal-based media could support cell growth, endospore production and larval toxicity induction of L. sphaericus.
Isolation of Bacillus subtilis from Several Locations in Mataram and its Toxicity to Aedes aegypti Mosquitoes in the Laboratory Alya Supriyani; Laila Annisa Rahmah; Sarkono Sarkono; Galuh Tresnani; Bambang Fajar Suryadi
Samota Journal of Biological Sciences Vol 5 No 1 (2025): Samota Journal of biological Sciences
Publisher : University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/kc6ken06

Abstract

One way to biologically control mosquitoes is by utilizing bacteria. Bacillus thuringiensis and Bacillus sphaericus are commonly used bacteria for this purpose. However, Bacillus subtilis has recently attracted the attention of researchers, as it is reported to have potential for use as a biopesticide. In this study, B. subtilis was isolated from several habitats in Mataram and West Lombok and tested on Aedes aegypti larvae to determine its potential toxicity. B. subtilis was isolated from the soil using a heating technique to separate it from other bacteria that do not have endospores and then grown at 33oC for 24 hours. The bacteria that grew were then tested on third-instar Ae. aegypti larvae. From several sampled locations, eight B. subtilis isolates with low toxicity levels were obtained. Toxic B. subtilis isolates were obtained from garden soil (isolate LT2C, 20%) and rhizosphere soil (isolate LB1A, 30%). The toxicity obtained is lower than the toxicity of B. thuringiensis and B. sphaericus, therefore further exploration is needed to be able to utilize B. subtilis as a biopesticide agent.
Identification of Potentially Pathogenic Bacteria in Fresh Marine Fish Sold at Kebon Roek Market, Mataram Baiq Kesy Juliani; Sarkono; Bambang Fajar Suryadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.19823

Abstract

This study aimed to enumerate, detect, and identify potentially pathogenic bacteria contaminating fresh marine fish sold at Kebon Roek Market, Mataram City. This preliminary laboratory-based descriptive survey focused on the fish species most frequently purchased by consumers: eastern little tuna (Euthynnus sp.), sardinella (Sardinella sp.), and Indian mackerel (Rastrelliger sp.). Fish samples were collected from nine purposively selected vendors located in different sections of the market, comprising three vendors each from the front, middle, and rear areas. Laboratory analyses included determination of the Total Plate Count (TPC), bacterial isolation using selective media, and bacterial identification based on cellular morphology, Gram-staining reactions, and biochemical characteristics. The bacterial loads were 3.36 × 10⁶ CFU/g in eastern little tuna, 1.76 × 10⁶ CFU/g in Indian mackerel, and 4.85 × 10⁵ CFU/g in sardinella. The bacterial counts detected in eastern little tuna and Indian mackerel exceeded the maximum limits specified in the Indonesian National Standard SNI 7388:2009. Bacterial identification revealed the presence of potentially pathogenic bacteria, including Enterobacter spp., Citrobacter spp., Escherichia coli, Edwardsiella spp., Vibrio spp., and Aeromonas spp. These findings indicate that fresh marine fish sold at Kebon Roek Market may serve as a vehicle for transmitting pathogenic bacteria to consumers. Therefore, improved hygiene and sanitation practices are required throughout the fish distribution and retail chain to ensure food safety.
Co-Authors Ahmad Jupri AIDA MUSPIAH AIDA MUSPIAH Alya Supriyani Anak Agung Putu Sidhiawan Baiq Kesy Juliani Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Setiaji Bambang Setiaji Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Faturrahman Faturrahman Faturrahman Galuh Trenani Galuh Tresnani Galuh Tresnani Ika Mustikasari Ika Nurhimaya Immy Suci Rohyani Islamul Hadi ISLAMUL HADI Juarsahnim Juarsahnim Jurnal Pepadu Laila Annisa Rahmah Langkah Sembiring Langkah Sembiring Moh. Andi Setiawan Nisful Mahdi Nur Indah Julisaniah Nur Indah Julisaniah Nurul Zulfa Fitriana Qothrunnada Amira Salma Rosal Okjayansah Sukarti Moeljopawiro Sukarti Moeljopawiro Sukiman . Suryadi, Bambang Fajar Yuliadi Zamroni Zuriatun Annisa