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All Journal Jurnal Biologi Udayana agriTECH Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Abdi Insani Bioscientist : Jurnal Ilmiah Biologi Proceeding Biology Education Conference Journal of Science and Science Education (JoSSEd) Jurnal Warta Desa (JWD) Prosiding Konferensi Nasional PKM-CSR Jurnal Pijar MIPA Jurnal Pepadu
Sarkono Sarkono
Program Studi Biologi Fakultas MIPA Universitas Mataram, Mataram, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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PELATIHAN PEMBUATAN DAN APLIKASI PUGATI UNTUK BIBIT GAHARU PADA KELOMPOK WANITA TANI DEDARE GAWAH DI PUSUK LESTARI LOMBOK BARAT Ernin Hidayati; Sarkono Sarkono; Juarsahnim Juarsahnim; Moh. Andi Setiawan; Bambang Fajar Suryadi; Faturrahman Faturrahman
Jurnal Abdi Insani Vol 10 No 2 (2023): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v10i2.898

Abstract

Currently, an ex-situ agarwood conservation effort was conducted in the Pusuk Lestari Forest, West Lombok by importing the plant varieties from outside the area. The difference of climate between the origin and destination areas makes the seedlings susceptible to stress and so they need to be acclimatized. Microbial organic-biological fertilizer (PUGATI) is applied to healthy and stimulating the growth of the seedlings during the acclimatization process. Microbial organic-biological fertilizer (PUGATI) is applied to healthy and stimulating the growth of the seedlings during the acclimatization process. This activity aims to increase the knowledge and skills of the Dedare Gawah Women Farmers Group in Pusuk Lestari Village to produce and application of PUGATI. The activity was carried out in November 2022 at Pusuk Lestari Village. The main activities are carried out in four steps: knowledge transfer by lectures, skills transfer by group practice and PUGATI applications, and also planting agarwood seedlings. The lecture was delivered by the dedication team. The team of community service presented a lecture. The practice of producing PUGATI is carried out in groups. The PUGATI application is carried out directly on the gaharu seedlings at the time of planting in the Pusuk Lestari Forest. Participants took part in all steps of the activities enthusiastically characterized by the many questions raised from the participants. Participants are able to carry out the practice of produced PUGATI correctly and precisely. All participants appreciated this activity because the knowledge and skill of PUGATI applicable not only to agarwood seedlings but also to other plants. This is in line with the participant daily activities, such as planting vegetables and fruit. Participants considered that this activity was very useful because it provided new knowledge and skills for them.
Eksplorasi Potensi Bacillus spp. sebagai Bakteri Pemacu Pertumbuhan Tanaman di Hutan Primer Resort Kembang Kuning Anak Agung Putu Sidhiawan; Sukiman Sukiman; Sarkono Sarkono; Ernin Hidayati; Bambang Fajar Suryadi
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8403

Abstract

Kembang Kuning resort is one of the Mount Rinjani National Park Resorts that have high biodiversity, so it has the potential for plant growth-promoting bacteria. Bacillus sp. is one species of bacteria that has great potential as a plant growth-promoting bacteria. The purpose of this study was to isolate and determine the ability of phosphate solubilization, nitrogen-fixing and anti-fungal activity of Bacillus spp. isolated from the Primary Forest of Kembang Kuning Resort, Gunung Rinjani National Park. This research is an exploratory study consisting of the isolation of Bacillus sp., phosphate solubilization test with spot inoculation method on Pikovskaya Agar media, qualitative nitrogen fixing test using Jensen's Nitrogen - Free media, and anti-fungal activity test against Fusarium sp. with dual culture method. There were 21 isolates of the genus Bacillus successfully isolated in this study. The phosphate solubilization test showed that 16 bacterial isolates could solubilize phosphate, where the highest phosphate solubilization index was produced by isolate P3.1 namely 0,52. A total of one isolate, namely isolate P3.1, can fix nitrogen on Jensen's Nitrogen-Free medium. In the anti-fungal test against Fusarium sp., three bacterial isolates were obtained that were able to inhibit the growth of Fusarium sp., where the highest antagonism was produced by isolate P2.8 which was 46,66%. Bacillus spp. isolated from Kembang Kuning Resort has potential as PGPB in terms of crop protection.
Isolation and Identification of Acid-Forming Bacteria in Arabica Coffee Fruit (Coffea arabica) During Wet Fermentation Qothrunnada Amira Salma; Sarkono Sarkono; Aida Muspiah
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.7335

Abstract

The distinctive flavor and aroma possessed by Arabica coffee are an attraction for coffee enthusiasts. Post-harvest processing of coffee such as coffee fruit fermentation also contributes to the formation of the flavor and aroma of Arabica coffee. In this study, spontaneous fermentation was conducted by adding distilled water, with treatments on fermentation days 4 and 7 aimed at detecting acid-forming bacteria that played a role in the fermentation process. The bacterial isolates were characterized through macroscopic and microscopic observations, physiological, and biochemical tests. Identification of bacterial isolates was performed using the profile matching method based on Bergey’s Manual of Systematic Bacteriology (Holt et al., 1994) and Axellson (2004) until the genus level. The identified bacterial genera were Asaia, Gluconobacter, Neoasaia, Saccharibacter, and Lactobacillus.
Fishmeal-Based Media Supports Growth and Endospore Production of Locally-Isolated Lysinnibacillus sphaericus and Induces its Toxicity to 3rd Instar Aedes aegypti Larvae in Laboratory Conditions Bambang Fajar Suryadi; Ika Mustikasari; Zuriatun Annisa; Sarkono; Galuh Tresnani
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.7361

Abstract

The aim of this study was to determine whether fishmeal-based media could be used to grow L. sphaericus and induce its toxicity against Aedes aegypti larvae. Three concentrations (10, 20 and 30%) of fishmeal-based media were used to grow L. sphaericus isolate Bs2-1-2. Cell growth and endospore production were observed every 12 hours for 72 hours. The lethal concentration was measured every 24 hours for 72 hours of fermentation. The highest cell concentration was found in L. sphaericus grown on the media with 30% fishmeal concentration (3.03x1008 cells/mL), followed by 20% concentration (2.43x1008 cells/mL) and the lowest at 10% concentration (2.20x1008 cells/mL). At the end of fermentation, the highest concentration was found in L. sphaericus grown on 30% fishmeal-based media (1.51x1008 cells/mL), followed by 20% media (6.95x1007 cells/mL) and 10% media (3.21x1007 cells/mL). After 72-hour incubation, the highest endospore concentration was achieved by L. sphaericus grown on 20% (2.51x1008 cells/mL) and 10% (2.19x1008 cells/mL) fishmeal-based media. Initial larval toxicity of L. sphaericus showed the highest mortality on 20 and 30% fishmeal-based media (both reaching 53.33%), while 10% fishmeal-based media gave only 26.67% larval mortality. The LC50 value at 72 hours was achieved by L. sphaericus cultured on 30% fishmeal-based media (2.47 x 1008 cells/mL), followed by 20% concentration (4.82 x 1008 cells/mL) and 10% concentration (9.01 x 1009 cells/mL). The conclusion of this study was all concentrations of fishmeal-based media could support cell growth, endospore production and larval toxicity induction of L. sphaericus.
Analisis bakteri rhizosfer pelarut fosfat pada tanaman jagung dari wilayah Empang Sumbawa, NTB Ernin Hidayati; Rosal Okjayansah; Faturrahman Faturrahman; Bambang Fajar Suryadi; Sarkono Sarkono; Sukiman Sukiman; Nur Indah Julisaniah
Jurnal Biologi Udayana Vol 28 No 1 (2024): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2024.v28.i01.p10

Abstract

Bakteri pelarut fosfat merupakan komponen ekosistem tanah yang berperan dalam membantu menyediakan fosfat bagi tanaman. Sampai saat ini, eksplorasi bakteri pelarut fosfat yang berasosiasi dengan perakaran tanaman terus dilakukan untuk mendapatkan isolat potensial. Tujuan penelitian ini adalah menganalisis bakteri rhizosfer pelarut fosfat pada tanaman jagung dari wilayah Empang Sumbawa, Nusa Tenggara Barat. Bakteri diisolasi dari tanah perakaran tanaman jagung yang berada pada fase pertumbuhan vegetatif dan fase generatif. Isolat diuji kemampuannya dalam melarutkan fosfat menggunakan medium Pikovskaya Agar. Pengujian dilakukan dengan teknik inokulasi titik. Zona bening yang terbentuk disekitar koloni bakteri mengindikasikan bahwa bakteri mampu melarutkan fosfat. Sebanyak 18 isolat bakteri rhizosfer berhasil diisolasi, 11 diantaranya menunjukkan kemampuan melarutkan fosfat. Isolat V6 dan V7 mempunyai kemampuan terbaik dibandingkan isolat lainnya, ditunjukkan dengan nilai indeks pelarutan fosfat sebesar 1,3 dan 1,54.
Co-Authors Ahmad Jupri AIDA MUSPIAH AIDA MUSPIAH Anak Agung Putu Sidhiawan Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Fajar Suryadi Bambang Setiaji Bambang Setiaji Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Ernin Hidayati Faturrahman Faturrahman Faturrahman Faturrahman Faturrahman Galuh Trenani Galuh Tresnani Ika Mustikasari Ika Nurhimaya Immy Suci Rohyani Islamul Hadi ISLAMUL HADI Juarsahnim Juarsahnim Jurnal Pepadu Langkah Sembiring Langkah Sembiring Moh. Andi Setiawan Nisful Mahdi Nur Indah Julisaniah Nur Indah Julisaniah Nurul Zulfa Fitriana Qothrunnada Amira Salma Rosal Okjayansah Sukarti Moeljopawiro Sukarti Moeljopawiro Sukiman . Suryadi, Bambang Fajar Yuliadi Zamroni Zuriatun Annisa