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KEEFEKTIFAN PUNTUNG ROKOK SEBAGAI PENGENDALI Gloeosporium fructigenum PADA BUAH APEL ( EFFECTIVITY OF CIGARETTE BUTTS AS CONTROL AGENT OF Gloeosporium fructigenum ON APPLE) Suharti, Woro Sri; Wachjadi, Muljo; Feti, Ruth
Pembangunan Pedesaan Vol 10, No 2 (2010)
Publisher : Pembangunan Pedesaan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The objective of the research was to determine the effectivity of cigarette butts extract to control the growth of Gloeosporium fructigenum causing apple bitter rot in vitro and in vivo. Randomized block design was used with factorial pattern for both in vitro and in vivo treatments. The first factor for in vitro treatment was kinds of solvent, i.e., water and ethanol. The second one was type of cigarette consisted of filtered cigarette butts, non-filtered cigarette butts and sliced tobacco. The third factors were the concentration of cigarette butts extract, and sliced tobacco (10, 30, and 50%). The first factor for in vivo treatment was concentration of non-filtered cigarette butts extract with water solvent (10, 20, and 30%), the second one was fruit soaking time (10, 15, and 20 minutes). Variables measured on in vitro research were diameter of the fungi, conidia size, and inhibition of conidial growth. Variables measured on in vivo one were incubation period, area of symptoms, effectiveness of cigarette butts extracts, rate of infection, and sensory test. The research results showed that the extract of non-filtered cigarette butts and sliced tobacco both with water and ethanol as a solvent had ability to inhibit the growth of G. fructigenum in vitro. Filtered cigarette butts extract was effective to control the G. fructigenum in vivo
Various pH Media Influence Production of Pseudomonas fluorescens P20 Raw Secondary Metabolites for Controlling Damping-off (Pythium sp.) in Cucumber Seedlings Loekas Soesanto; Siti Latifah; Abdul Manan; Endang Mugiastuti; Woro Sri Suharti
AGRIVITA, Journal of Agricultural Science Vol 44, No 1 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i1.2730

Abstract

 This research aims to determine the best pH media for the production of Pseudomonas fluorescens P20 raw secondary metabolites, its effect on controlling damping-off, and on cucumber seedling growth. In vitro test uses completely randomized design with four replicates and seven treatments consisted of pH 5.0; 5.5; 6.0; 6.5; 7.0; 7.5; and 8.0. In planta test uses a randomized block design with three replicates and ten treatments consisting of control, mancozeb 80%, and raw secondary metabolites with pH 7.0 and 7.5, and 4 concentration levels, i.e., 5, 10, 15, and 20%. Variables observe population density, inhibition ability, protease and chitinase qualitatively, germination ability, incubation period, disease incidence, the area under disease progress curve (AUDPC), crop height, number of leaves, root length, and crop fresh weight. The result shows that the best pH for the production of raw secondary metabolites is 7.0, indicated by population density as 5.68 × 1024 cfu/ml, inhibition ability as 50.8%, and the best protease and chitinase qualitatively. Application of the secondary metabolites on pH 7.0 could suppress disease intensity, incubation period, and AUDPC as 66.67, 77%, and 0%-day, respectively, and increase crop height, the number of leaves, root length, and crop fresh weight as 57.65, 37.19, 63, and 74%, respectively.
Nutrient Uptake, Chlorophyll Content, and Yield of Rice (Oryza sativa L.) Under the Application of PGPR Consortium Purwanto Purwanto; Woro Sri Suharti
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 3 (2021): December 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i3.31990

Abstract

Indigenous paddy soil rhizobacteria are one alternative to restore biological fertility and soil health. Plant Growth Promotion Rhizobacteria (PGPR) that act as biofertilizer will help to increase the availability of nutrients and promote the plant growth. The objective of this research was to study the effect of the PGPR consortium indigenous paddy soil to nutrient uptake, chlorophyll content, and yield of rice. This research was arranged by Randomized Block Design with the treatment was the combination of plant Growth Promotion Rhizobateria isolates originated from paddy soil. The treatments consisted of control, Rhizobium sp. LM-5, R08 isolate, R011 isolate, Rhizobium sp. LM-5 + R08 isolate, Rhizobium sp. LM-5 + R011 isolate, R08 + R011 isolates, Rhizobium sp. LM-5 + R08 + R011 isolates. The result showed that the application of the PGPR consortium was able to increase the root growth of rice plants thereby increasing nutrient uptake, leaf chlorophyll content, and plant biomass. Application of single strain R11 isolate and the consortium of Rhizobium sp. LM-5 + R11 isolate were capable of giving the highest grain yield of 64.99 and 62.80 g plant-1 respectively. These finding were PGPR consortium between IAA-producing bacteria combined with Rhizobium sp. LM-5 as N2 fixing bacteria in increasing nutrient uptake, chlorophyll contents and crop yields, it can be recommended that PGPR consortium as a biofertilizer formula in rice cultivation
Uji Viabilitas dan Virulensi Fusarium oxysporum f.sp. zingiberi Isolat Boyolali dan Temanggung Setelah Disimpan Tujuh Belas Tahun dalam Tanah Steril Soesanto, Loekas; Fakhiroh, Zaqiatul; Suharti, Woro Sri
Jurnal Fitopatologi Indonesia Vol. 18 No. 2 (2022): Maret 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.2.91-99

Abstract

Viabilitas dan Virulensi Fusarium oxysporum f. sp. zingiberi Asal Boyolali dan Temanggung Setelah Disimpan Tujuh Belas Tahun di Dalam Tanah Steril Fusarium oxysporum f. sp zingiberi merupakan cendawan penyebab busuk rimpang pada jahe. Patogen ini dapat bertahan di tanah selama bertahun-tahun tanpa tanaman inang. Penelitian bertujuan untuk menguji viabilitas dan virulensi 21 galur F. oxysporum f. sp. zingiberi setelah disimpan tujuh belas tahun di dalam tanah steril. Variabel yang diamati adalah warna dan diameter koloni, bentuk makrokonidium dan mikrokonidium, waktu pertumbuhan, berat kering miselium, kepadatan konidium, masa inkubasi, luas serangan pada rimpang, selisih bobot basah rimpang, dan indeks sampah. Hasil penelitian menunjukkan bahwa semua galur F. oxysporum f. sp. zingiberi asal Temanggung dan Boyolali yang disimpan di tanah steril selama 17 tahun mampu tumbuh baik pada medium PDA dan mengisi penuh cawan petri antara 11–36 hari. Selain itu, semua galur menyebabkan gejala penyakit pada rimpang jahe var. Gajah. Isolat dengan tingkat virulensi yang rendah ditandai oleh masa inkubasi yang panjang (6–12 hari setelah inokulasi) dan luas area rimpang terserang yang terkecil.
PENGOLAHAN SAMPAH ORGANIK MENJADI ECO-ENZYME DI DESA KOTAYASA, SUMBANG, BANYUMAS GUNA MENGATASI PERMASALAHAN SAMPAH DAN MENINGKATKAN PEREKONOMIAN MASYARAKAT Heryawati, Amelia Putri; Adijana, Reksa Satria; Muthia, Difa Sri; Faiq, Daffa Naufal; Suharti, Woro Sri
Jurnal Dinamika Pengabdian Vol. 9 No. 2 (2024): JURNAL DINAMIKA PENGABDIAN VOL. 9 NO. 2 MEI 2024
Publisher : Departemen Budidaya Pertanian Fakultas Pertanian UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jdp.v9i2.21277

Abstract

Desa Kotayasa, Kecamatan Sumbang, Kabupaten Banyumas merupakan desa yang memiliki permasalahan kemiskinan dan sampah yang menumpuk namun belum ada pengangkutan dan pengolahan sampah. Terdapat potensi dengan pengolahan sampah yang akan meningkatkan perekonomian masyarakat dan mengatasi masalah sampah. Produksi sampah organik dan anorganik cukup banyak setiap harinya, terutama sampah yang berasal dari pasar. Sampah pasar sebagian besar adalah sampah organik. Salah satu cara penanganan sampah organik yaitu dengan mengolahnya menjadi eco-enzyme. Eco-enzyme bermanfaat sebagai pembersih serba guna, sebagai pupuk untuk tanaman serta dapat digunakan sebagai pestisida alami untuk mengendalikan penyakit dan hama tanaman. Tujuan dari kegiatan ini adalah:                      1) Sosialisasi pembuatan eco-enzyme skala rumah tangga; 2) Pelatihan pembuatan eco-enzyme skala rumah tangga; 3) Pelatihan pembuatan eco-enzyme skala besar. Tahapan kegiatan meliputi: 1) Sosialisasi pembuatan eco-enzyme skala rumah tangga; 2) Pelatihan pembuatan eco-enzyme skala rumah tangga; 3) Pelatihan pembuatan eco-enzyme skala besar; dan 4) Evaluasi kegiatan. Hasil yang dicapai dari kegiatan pengabdian di Desa Kotayasa yaitu sosialisasi mengenai eco-enzyme yang tersampaikan dengan baik, praktik pembuatan eco-enzyme sebanyak 2 tong yang diamati oleh masyarakat. Kata kunci: Banyumas, Desa Kotayasa, Eco-enzyme, sampah organik. ABSTRACT Kotayasa Village in Sumbang Banyumas District is a village that has problems of poverty and garbage that has accumulated. However, there is no transportation and processing of waste. There is potential for waste processing which will improve the community's economy and overcome the waste problem. The production of organic and inorganic waste is quite a lot every day, especially waste that comes from the market. Market waste is mostly organic waste. One way to handle organic waste is to process it into eco-enzymes. Eco-enzyme is useful as a general-purpose cleaner, as a fertilizer for plants and can be used as a natural pesticide to control plant diseases and pests. The objectives of this activity are: 1) Dissemination of household-scale eco-enzyme production; 2) Training on household-scale for eco-enzyme production; 3) training on large-scale for eco-enzyme production. The activity stages include: 1) Dissemination of household-scale eco-enzyme production; 2) household-scale eco-enzyme production training; 3) Large-scale eco-enzyme production training; and 4) Activity evaluation. The results achieved from the community service activities in Kotayasa Village were socialization of the eco-enzyme which was well conveyed, the practice of making 2 barrels of eco-enzyme which was observed by the community. Keywords: Banyumas, eco-enzyme, organic waste, Kotayasa Village.
The Effect of Various pH Medium on the Secondary Metabollites Production from Trichoderma harzianum T10 to Control Damping Off on Cucumber Seedlings Chalimah, Nur; Soesanto, Loekas; Suharti, Woro Sri
Journal of Tropical Horticulture Vol 3, No 2 (2020): October 2020
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v3i2.52

Abstract

Damping-off is one of the main diseases in cucumber seedlings caused by Pythium sp. Secondary metabolites of Trichoderma harzianum T10 can conduct the control of the disease. The pH of the medium influences the production of secondary metabolites. The research aimed to determine the effective pH medium on production of T. harzianum T10 secondary metabolites, and the effect of the T. harzianum T10 secondary metabolites application in damping-off disease control also to the growth of cucumber seedling. The research was consist of two steps; 1) in vitro assay with various pH levels 5; 3; 3.5; 4; 4.5; 5.5; 6; 6.5; and 7, 2) In planta treatments consisted of control, fungicide (Mancozeb), secondary metabolites in pH 5 and 5.5 with the concentration of 5, 10 and 15% each. The research showed that: 1) the effective pH medium for the production of T. harzianum T10 secondary metabolites was 5 and 5.5. 2) application of the T. harzianum T10 secondary metabolites on pH 5 and 5.5 with a concentration of 5, 10, and 15% could decrease the disease incidence and support cucumber seedling growth.
Application of Bio P60 and Bio T10 Alone or in Combination Against Stem Rot of Pakcoy Soesanto, Loekas; Hiban, Atsil; Suharti, Woro Sri
Journal of Tropical Horticulture Vol 2, No 2 (2019): October 2019
Publisher : Yayasan Pertanian Tropika Indonesia (YPTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1104.904 KB) | DOI: 10.33089/jthort.v2i2.20

Abstract

Application of Bio P60 and Bio T10 alone or in combination in suppressing stem rot of pakcoy and on pakcoy growth has been demonstrated. The research was carried out at the Plant Protection Laboratory and Screen House, Faculty of Agriculture, Jenderal Soedirman University. A split-plot design was used with application time (before and after inoculation of Sclerotium rolfsii) as main plot and Bio P60, Bio T10, and Bio P60 + Bio T10) as sub-plot. Observed variables were incubation period, disease intensity, crop height, number of leaves, and crop fresh weight. Results of the research showed that single and combined application of Bio T10 and Bio P60 did not differ in the suppression of stem-end rot in pakcoy. The combination of Bio T10 + Bio P60 was able to control the disease by delaying the incubation period and suppressing the disease intensity respectively by 37.48-39.16% and 54.77-6191% compared to controls. Combined Bio T10 + Bio P60 was able to improve plant height, number of leaves, and fresh weight of plants as 29.99-46.62, 24.39-35.07, and 71,17%, respectively, compared to controls. The results of this study suggest that the raw secondary metabolites of Bio P60 and Bio T10 either alone or in combination could be applied for the prevention or treatment of the diseases in pakcoy.
Salicylic Acid effectiveness as Resistance Inducer of Rice Plant Against Sheath Blight Pathogen Suharti, Woro Sri
Agrotechbiz : Jurnal Ilmiah Pertanian Vol 10 No 1 (2023): Agrotechbiz: JurnL Ilmiah Pertanian
Publisher : Universitas Panca Marga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51747/agrotechbiz.v10i1.1179

Abstract

This study aims to determine the effectiveness of salicylic acid in inhibiting the growth of Rhizoctonia solani in vitro and to determine the effect of salicylic acid in increasing rice plants' resistance to rice sheath blight disease. In the in vitro research stage, the antifungal activity of salicylic acid was assayed with 5 levels of concentration. The observed variable was the percentage of R. solani mycelium growth inhibition. The next stage of research was carried out in planta. The observed variables in the resistance assay were the pathosystem, growth, morphological, and physiological components. Based on the observed variables of pathosystem components, salicylic acid can reduce the intensity of rice sheath blight both in vitro and in planta. Based on observations of morphological and physiological components, the salicylic acid increased plant resistance by thickening the leaf epidermis and increasing the phenolic compounds.
Application of Biocontrol Product Bio P60 and NASA Liquid Organic Fertilizer on The Development of Fusarium Wilt and Yield of Shallot in Planta Soesanto, Loekas; Yusup, Adi Maulana; Sastyawan, Murti Wisnu Ragil; Mugiastuti, Endang; Suharti, Woro Sri
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.24558

Abstract

Shallots are indispensable in all aspects of human life, and shallot production is always facing Fusarium wilt disease. Chemical control of the disease has failed, and environmentally friendly control alternatives are needed. One of them is Bio P60 biocontrol product combined with liquid organic fertilizer. Aims of the research was to assess the effectiveness of NASA liquid organic fertilizer and Bio P60 in controlling Fusarium wilt and its impact on shallot growth and yield in planta. This research was conducted in the field using polybag, for five months. Completely randomized block design was used comprising of two components with 16 treatments and 3 replicates. The variables observed were incubation period, disease intensity, AUDPC, plant height, number of leaves, number of tubers, plant wet weight, tuber wet weight, plant dry weight, and tuber dry weight. The results showed that the application of Bio P60 five times was the most excellent treatment in postponing the incubation period by 61.71%, suppressing disease incidence by 66.67%, and reducing AUDPC by 69.84%, increase growth and yield components such as plant height by 30.75%, number of leaves by 40.7%, number of bulbs by 75.6%, bulb fresh weight by 104.53%, blub dry weight by 51.1%, crop fresh weight by 48.24%, and crop dry weight by 49.77% compared to the control. The fertilizer application has no significant effect on all variables. There was no interaction between Bio P60 and NASA on all variables.
PENERAPAN PEMUPUKAN TANAMAN BUAH DALAM POT DI SDIT MUTIARA ILMU SOKARAJA UNTUK MENDUKUNG KELESTARIAN LINGKUNGAN SEKOLAH Tini, Etik Wukir; Suharti, Woro Sri; Andreas, Roy
Jurnal Dinamika Pengabdian Vol. 5 No. 1 (2019): JURNAL DINAMIKA PENGABDIAN VOL. 5 NO. 1 OKTOBER 2019
Publisher : Departemen Budidaya Pertanian Fakultas Pertanian UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jdp.v5i1.8119

Abstract

ABSTRAKKegiatan pengabdian yang dilakukan antara lain transfer pengetahuan dan teknologi dengan cara penyuluhan dan praktek langsung berupa pelatihan meliputi: pelatihan pemeliharaan tanaman buah dalam pot dan pemupukan tanaman buah dalam pot. Pelatihan sosialisasi teknik penanaman tanaman buah dengan bibit unggul dan pemupukan NPK dan pupuk daun pada tanaman buah, upaya untuk meningkatkan pertumbuhan dan produksi pada tanaman. Tanaman buah yang ditanam dalam pot antara lain jambu air, jambu biji, kelengkeng, belimbing, jeruk dan mangga. Metode yang diterapkan adalah ceramah, praktek langsung, dan pelatihan dengan dibuat kelompok yang masing-masing anggotanya berjumlah 5 sampai 6 orang. Target luaran peningkatan pengetahuan tentang budidaya pembibitan tanaman jambu air citra dan belimbing yang baik dan efisien sebesar di atas 70%, peningkatan keterampilan tentang budidaya pembibitan tanaman jambu air citra dan belimbing yang efisien dan baik sebesar 70%, dan peningkatan optimalisasi lahan pekarangan dengan penanaman tanaman buah dalam pot sebesar 40%. Hasil yang dicapai dari kegiatan pengabdian sebagai berikut: 1) Kegiatan pengabdian berbasis riset telah dilaksanakan dengan baik dan mendapat respon yang positif dari murid, Kepala Sekolah, guru, wali murid, maupun karyawan SDIT Mutiara Ilmu Sokaraja; 2) Pemupukan adalah NPK 10 g/tanaman jambu biji dan jeruk, 20 g/tanaman jambu air, 30 g/tanaman mangga, 40 g/tanaman belimbing, dan pupuk daun 1 g/l; dan 3) SDIT Mutiara Ilmu Sokaraja menginginkan kegiatan lanjutan berupa membungakan bibit tanaman buah di dalam pot, sehingga diharapkan kegiatan pengabdian dapat dilaksanakan untuk tahun kedua (pada tahun 2019).Kata Kunci: Tanaman Buah dalam Pot, Pemupukan. ABSTRACTThe activities carried out include transfer of knowledge and technology by means of counseling and direct practice in the form of training including: training in the maintenance of fruit trees in pots and fertilizing fruit plants in pots. Training on socialization of techniques for planting fruit trees with superior seeds and fertilizing NPK and leaf fertilizers on fruit trees, efforts to increase growth and production in plants. Fruit plants planted in pots include water guava, guava, longan, star fruit, oranges and mangoes. The methods applied were lectures, direct practice, and training with groups of 5 to 6 student. Output targets for increasing knowledge of good and efficient guava seedling and sta rfruit plant nursery cultivation are above 70%, improvement in skills of efficient and good image guava plant nursery and star fruit cultivation by 70%, and increased optimization of yard area by planting plants fruit in pots by 40%. The results of dedication are as follows: 1) Research-based service activities have been carried out well and received positive responses from students, principals, teachers, guardians of students, and employees of SDIT Mutiara Ilmu Sokaraja, 2) Fertilizing NPK 10 g/plant to guava and oranges, 20 g /plant water to apple, 30 g/plant to mango, 40 g/plant to star fruit, and 1 g/l leaf fertilizer, and 3) SDIT Mutiara Ilmu Sokaraja wants further activities in the form of flowering fruit seeds in pots, so it is expected that service activities can be carried out for the second year (in 2019).Keywords: Fruit Plants in Pot, Fertilization.