Garuda - Garba Rujukan Digital

Dian Hasanah

Laboratorium Fisiologi, Fakultas Kedokteran, Universitas Brawijaya

Author-ID : 437580

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences

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1

Modifying Effect of Anthocyanin from Purple Sweet Potatoes on Visceral Fat Tissue Inflammation and Liver Oxidative Stress in Psychological Stress-Induced Mice: Purple Sweet Potatoes on Psychological Stress Nia Kurnianingsih; Dian Artamevia; Antania Kashira Winarta; Andini Putri Wulandari; Dian Hasanah; Edwin Widodo; Retty Ratnawati
Journal of Tropical Life Science Vol. 13 No. 2 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.02.18

Psychological stress generates inflammation and oxidative stress in various tissues. Visceral fat as the target site of inflammation is then correlated with stress-induced obesity. Redox imbalance following inflammation also has prominent impacts on hepatic tissue. Therefore, the development of anti-inflammatory and antioxidant properties from plant bioactive compounds is necessary to be investigated. Anthocyanin (ANC) from various plants is previously described as a powerful anti-inflammatory and antioxidant even though its effect in psychological stress remains underexplored. Purple sweet potatoes (PSP) has ANC as its natural pigment. Previous studies revealed the potential effect of ANC from PSP on behavior and an antidepressant candidate. Thus, this study was purposed to determine the effect of ANC from PSP on inflammation and oxidative stress in visceral fat and hepatic tissue respectively. A total of 25 adult male BALB/c mice were assigned into groups of control, stress, stress+ANC 10 mg/kgBW, stress+ANC 20 mg/kgBW and stress+ANC 40 mg/kgBW. Restraint stress was applied 2 hours/day for 14 days. Enzyme-linked immunoassay (ELISA) was conducted to measure level of IL-6 and IL-10 in visceral fat as well as SOD and MDA from hepatic tissue. The results demonstrated that the supplementation of ANC reduces the level of IL-6 cytokine (p=0.005), tends to increase IL-10 (p=0.612), reduces hepatic SOD (p=0.03), and does not significantly affect the level of hepatic MDA (p=0.432). Both ratios of IL-6/IL-10 and SOD/MDA were reduced following ANC administration. Total ANC extracts are suggested to have a potential role of resisting inflammation and oxidative stress in the psychologically stressed model. Further studies are necessary to evaluate the benefits of ANC from PSP in other peripheral organs under psychological stress exposure.

Alterations in Blaood Profiles and Spleen Tissue Following Purple Sweet Pota-to Anthocyanin Extract Treatment in Restraint-Stressed Mice: Blood Profiles and Spleen Tissue Following Purple Sweet Potato Anthocyanin Extract Treatment Kurnianingsih, Nia; Artamevia, Dian; Hasanah, Dian; Harbiyanti, Novita Titis; Ratnawati, Retty
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.19

Stress is associated with broad changes in blood and immune cell dysfunction, which are correlated with the spleen as a secondary lymphoid organ. Anthocyanin, a plant bioactive compound from purple sweet potatoes, has previously been reported to have a beneficial effect on stress response behavior. However, the limited studies evaluated anthocyanin's effect on blood profiles and spleen tissue under stress. Therefore, this study aimed to explore the effect of total anthocyanin extract (ANC) from purple sweet potatoes (PSP) on the blood profiles and spleen tissue of restraint stressed mice. A total of 20 male adult BALB/c mice were divided into control (CTRL), stress (STR), stress + ANC 10 mg/kgBW (STR+ANC10), stress + ANC 20 mg/kgBW (STR+ANC20), and stress + ANC 40 mg/kgBW (STR+ANC10). Restraint stress was applied two hours/day for 14 days. An automatic blood analyzer was used for blood profiling. Immunoblotting of caspase-3 and hematoxylin-eosin-stained histological slides were observed for spleen tissue analysis. Combination of STR and ANC treatment reduced the erythrocyte count, hemoglobin level, and leucocyte count. The ANC-treated groups had a higher percentage of lymphocytes and a lower percentage of granulocytes. The expression of caspase-3 declined among ANC-treated groups. Spleen extramedullary erythropoiesis, vacuolization, and white pulp involution were demonstrated in stress and ANC-treated animals. In summary, restraint stress modified the blood cell profiles and spleen tissue in the mice model. Further research is necessary to evaluate the mechanism of ANC during stress on blood cells and splenic tissue repair enhancement.

Alterations in Blaood Profiles and Spleen Tissue Following Purple Sweet Pota-to Anthocyanin Extract Treatment in Restraint-Stressed Mice: Blood Profiles and Spleen Tissue Following Purple Sweet Potato Anthocyanin Extract Treatment Kurnianingsih, Nia; Artamevia, Dian; Hasanah, Dian; Harbiyanti, Novita Titis; Ratnawati, Retty
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.19

Stress is associated with broad changes in blood and immune cell dysfunction, which are correlated with the spleen as a secondary lymphoid organ. Anthocyanin, a plant bioactive compound from purple sweet potatoes, has previously been reported to have a beneficial effect on stress response behavior. However, the limited studies evaluated anthocyanin's effect on blood profiles and spleen tissue under stress. Therefore, this study aimed to explore the effect of total anthocyanin extract (ANC) from purple sweet potatoes (PSP) on the blood profiles and spleen tissue of restraint stressed mice. A total of 20 male adult BALB/c mice were divided into control (CTRL), stress (STR), stress + ANC 10 mg/kgBW (STR+ANC10), stress + ANC 20 mg/kgBW (STR+ANC20), and stress + ANC 40 mg/kgBW (STR+ANC10). Restraint stress was applied two hours/day for 14 days. An automatic blood analyzer was used for blood profiling. Immunoblotting of caspase-3 and hematoxylin-eosin-stained histological slides were observed for spleen tissue analysis. Combination of STR and ANC treatment reduced the erythrocyte count, hemoglobin level, and leucocyte count. The ANC-treated groups had a higher percentage of lymphocytes and a lower percentage of granulocytes. The expression of caspase-3 declined among ANC-treated groups. Spleen extramedullary erythropoiesis, vacuolization, and white pulp involution were demonstrated in stress and ANC-treated animals. In summary, restraint stress modified the blood cell profiles and spleen tissue in the mice model. Further research is necessary to evaluate the mechanism of ANC during stress on blood cells and splenic tissue repair enhancement.

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