Claim Missing Document
Check
Articles

Found 12 Documents
Search

Study Biopotency of Rambusa (Passiflora foetida L.) Fruit Extract As An Antibacterial for Aeromonas hydrophila Fatmawati, Fatmawati; Hanafie, Agussyarif; Aisiah, Siti; Rini, Ririen Kartika; Olga, Olga; Agustina, Rizka; Aminah, Aminah; Kartiani, Elvina
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol. 14 No. 2: April 2026
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v14i2.15708

Abstract

Motile Aeromonad Septicemia (MAS) is a disease affecting freshwater fish and is caused by Aeromonas hydrophila. The control of MAS commonly relies on synthetic drugs and antibiotics. This study aimed to evaluate the biopotential of rambusa (Passiflora foetida L.) fruit extract as an antibacterial agent against A. hydrophila. The antibacterial activity was assessed using the disc diffusion and Minimum Inhibitory Concentration (MIC) methods, supported by qualitative and quantitative phytochemical analyses. The results showed that rambusa fruit extract produced inhibition zones against A. hydrophila. The inhibition zone of the aqueous extract after 24 hours of incubation was 3.25 mm, while the methanolic extract produced an inhibition zone of 11.42 mm. These findings indicate that rambusa fruit extract prepared using both distilled water and methanol as solvents can inhibit the growth of A. hydrophila. Qualitative phytochemical screening of the aqueous extract revealed the presence of flavonoids, alkaloids, tannins, saponins, and hydroquinones based on the 10% KOH test. Quantitative analysis also detected flavonoids, alkaloids, tannins, saponins, steroids, phenolics, anthocyanins, and triterpenoids. The MIC test showed that concentrations of 100–600 mg/L were effective in inhibiting the growth of A. hydrophila within the weak inhibition category, while concentrations of 700–900 ppm showed higher inhibitory activity. These results suggest that rambusa fruit extract has potential as a natural antibacterial agent against A. hydrophila.
Hematological and Histological Analysis of Tilapia (Oreochromis Niloticus) Cultured in Floating Net Cages After Disease Outbreak Ririen Kartika Rini; Siti Aisiah; Olga; Lutfia Nafisah
Jurnal Penelitian Pendidikan IPA Vol 10 No 4 (2024): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i4.5057

Abstract

Recently, Tilapia fish cultivated in floating net cages in Karang Intan District experienced a sudden death from June 4 to June 5. At some point, mortality reached 50% of the population in floating net cages. The purpose of this study was to analyze the hemtology and histology of tilapia (Oreochromis niloticus) after exposure to disease outbreaks that were cultivated in floating net cages. The research location was carried out at the Laboratory of Fish Pests and Diseases, Faculty of Fisheries and Maritime Affairs, University of Lambung Mangkurat. The animal samples used were tilapia showing clinical symptoms of sick fish obtained from floating net cages in Mali-Mali Village, Karang Intan District, which experienced quite high fish mortality cases and 3 fish were taken. Based on the results of hemtological analysis of tilapia (Oreochromis niloticus) after being exposed to disease outbreaks which were cultivated in floating net cages, the hemoglobin levels of tilapia obtained in this study ranged from 6.9-7.8 g/dl, the average hematocrit of tilapia was obtained in this study has a range between 37.2 - 58.9%, the average tilapia leukocrit obtained in this study has a range between 2.89 - 6.61% and blood plasma is a liquid component of blood consisting of 99% water and 8-9% protein.