Retno Dyah Puspitarini
Jurusan Hama Dan Penyakit Tanaman, Fakultas Pertanian, Universitas Brawijaya

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INVENTARISASI HAMA PADA TANAMAN SALAK MADURA Salacca zalacca (Gaertn.) Voss Ayu Kurnia Puspasari; Retno Dyah Puspitarini; Sri Karindah
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 8 No. 1 (2020)
Publisher : Universitas Brawijaya

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Abstract

Buah salak Madura yang berasal dari Kabupaten Bangkalan memiliki pemasaran agrowisata yang sukses. Sayangnya, serangan hama menurunkan produksi salak di Bangkalan, Madura. Penelitian ini bertujuan untuk mengetahui OPT, gejala kerusakan, dan keparahan penyakit salak Madura. Penelitian dilakukan di tiga petak lahan dengan melakukan observasi dan identifikasi hama umum pada salak. Enam tanaman sampel pada setiap plot ditentukan secara sistematis mengikuti pola garis tanaman. Organ buah salak yang diamati meliputi pelepah daun, daun, bunga, dan buah. Hasil penelitian ditemukan beberapa jenis hama di empat organ tumbuhan salak. Delapan belas kutu putih Pseudococcid (Homoptera), empat ulat penggerek daun salak noctuid (Lepidoptera), dan 31 kutu perisai Diaspidid (Homoptera) ditemukan pada daun. Sementara itu, tiga puluh dua kumbang penggerek bunga salak Curculionid (Coleoptera) terlihat pada bunganya. Enam belas kumbang pemakan buah Nitidulid (Coleoptera), delapan lalat buah Drosophilid (Diptera), dan lima puluh enam rayap isopteran ditemukan pada bagian buah. Selanjutnya, sembilan puluh delapan isopteran rayap juga ditemukan pada pelepah daun. Intensitas kerusakan absolut pada bunga yang disebabkan famili Curculionidae termasuk dalam kategori kecil. Kerusakan absolut pada buah akibat rayap termasuk dalam kategori sedang, sedangkan famili Nitidulidae dan famili Drosophilidae termasuk dalam kategori kecil. Kerusakan tidak mutlak pada pelepah daun akibat rayap termasuk dalam kategori tinggi dan kerusakan tidak absolut pada daun akibat famili Pseudococcidae termasuk dalam kategori kecil. Serangga lain yang ditemukan pada buah salak adalah lalat buah Tephritid (Diptera), pirate bugs anthocorid (Hemiptera), Pseudoscorpio arachnidan, tungau Erythrqeid (Acari), dan tungau Oribatid (Acari).
Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Muhammad Indar Pramudi; Retno Dyah Puspitarini; Bambang Tri Rahardjo
Journal of Tropical Life Science Vol. 3 No. 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
Effects of Compost on Nematode Pratylenchus sp. (Tylenchida: Pratylenchidae) Population in Patchouli Cecep Subarjah; Toto Himawan; Retno Dyah Puspitarini
Journal of Tropical Life Science Vol. 6 No. 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.07

Abstract

Patchouli is seen as one of the most important export commodities in Indonesia. The presence of parasitic nematode Pratylenchus sp. creates a barrier to plant cultivation. Increasing organic content in soil by applying compost in the plantation is one of approaches in controlling the nematode. This research was mainly aimed to study the effects of compost fertilization on the population growth of nematode Pratylenchus sp. and yields on patchouli. These research used a Completely Randomised Design (CRD) consisting of 12 treatments with three replicates. Selected plant was inoculated with nematodes with the following initial population: 100, 200, 300, 400, and 500 nematodes in soil with and without compost. The plant with no inoculation was used as control. The result of the research confirmed that the addition of compost was capable of suppressing the population of nematode Pratylenchus sp. as much as 58.93 - 67.31% and 40.74 - 59.19% in root and in soil of each treatment, respectively. Compost applied in each treatment positively affected the increasing amount of Patchouli oil yielded, and it could also reduce the loss as much as 18.34 - 43.36%. Trichoderma sp. was found to be dominant in compost in the treatment with its population of 2.1 × 104 propogul/g.
Populasi Elaeidobius kamerunicus Faust (Coleoptera: Curculionidae) pada beberapa umur tanaman kelapa sawit Rahardjo, Bambang Tri; Rizali, Akhmad; Utami, Ika Putri; Karindah, Sri; Puspitarini, Retno Dyah; Sahari, Bandung
Jurnal Entomologi Indonesia Vol 15 No 1 (2018): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.15.1.31

Abstract

Elaeidobius kamerunicus Faust is an oil palm pollinator insect that lives on the male inflorescence and visits female inflorescence to pollinate due to attracted by its volatile compound. The number of fruit sets of oil palm relates to the population of E. kamerunicus on a plantation. The objective of this study was to investigate the effect of age of oil palm on the population of E. kamerunicus. Field research was conducted in oil palm plantations that located in Pangkalan Lada, Kotawaringin Barat District, in Central Kalimantan. The research method was by measurement of E. camerunicus population on male inflorescences and set up the sticky trap on female inflorescences. Plot with size 7000 m2 (100 trees) was selected on several ages of oil palm i.e. 6, 10 and 16 years. Each plot, the number of male and female inflorescences was counted and some inflorescences were chosen for measurement of E. camerunicus population that was conducted monthly for three months of observation. The results showed that the age of the oil palm affected the population of E. kamerunicus in male inflorescences but not in female inflorescences. The older age of oil palm, the population of E. kamerunicus in male flowers was increasing. The sex ratio of E. kamerunicus found in male and female inflorescences of oil palms tend to female bias. Based on the comparison of E. kamerunicus population between male and female inflorescences on each plot, it was found that visitation value of E. kamerunicus was highest in the young oil palm. In conclusion, increasing age of oil palm trees affected on increasing of E. kamerunicus population in male inflorescences, but visitation value on female inflorescences prone to decline.
Diversity of Entomopathogenic Fungi in Rice Field Bunds: Role of Soil Properties and Weed Vegetation for Integrated Pest Management Afandhi, Aminudin; Pamungkas, Bayu Aji; Rachmawati, Rina; Syib’li, Muhammad Akhid; Karina, Anisya Putri; Fernando, Ito; Puspitarini, Retno Dyah; Setiawan, Yogo
AGRIVITA Journal of Agricultural Science Vol 47, No 3 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i3.4506

Abstract

Rice field bunds function as a reservoir of biodiversity, one of which is entomopathogenic fungi (EPF), which plays a role in controlling insect populations and increasing plant resistance. This study aimed to explore the interaction between organic matter content, soil pH, and EPF diversity in rice fields from Mulyoagung Subdistrict, Malang Regency. EPF was isolated using the insect bait method, followed by pathogenicity testing on Tenebrio molitor larvae. The Shannon-Wiener index (H'), evenness (E), and dominance (C) were used to assess the diversity of EPF on rice field bunds. Spearman correlation analysis was conducted to test the relationship between organic matter content, soil pH, and EPF diversity. Based on the results of the study, three genera of EPF were found in the rice fields, namely Beauveria, Fusarium, and Metarhizium. The presence of EPF is influenced by understory plants and the amount of organic matter in the soil. Therefore, preserving rice field bunds is very important to maintain the EPF population.
Local factors dominantly influence citrus red mite (Panonychus citri (McGregor)) population over landscape factors in Indonesian citrus orchards: Faktor lokal lebih dominan mempengaruhi populasi tungau merah jeruk (Panonychus citri (McGregor)) daripada faktor lanskap pada pertanaman jeruk di Indonesia Salamah, Luklu Is; Rizali, Akhmad; Puspitarini, Retno Dyah
Jurnal Entomologi Indonesia Vol 22 No 2 (2025): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.2.105

Abstract

The citrus red mite Panonychus citri (McGregor) is a major pest that can reduce the quality and yield of citrus crops. However, information regarding the local and landscape factors that influence its population dynamics remains limited. This study aimed to analyse the influence of local and landscape factors on citrus red mite population density. The research was conducted in nine citrus orchards in Malang, East Java, Indonesia. Data collected included local factors (pesticide application frequency and plant canopy density) and landscape factors (landscape composition, encompassing the number of patches (NP) and class area (CA) of semi-natural habitats). Pesticide application frequency data were obtained through farmer interviews, while canopy density was measured using a photographic method and analysed with ImageJ2 software. Landscape composition data were by digitizing land-use types within a 500-meter radius of the plot center using QGIS software and subsequently analysed using LecoS (QGIS add-ins) to obtain the NP and CA of semi-natural habitats. The generalized linear  model (GLM) analysis indicated no overall significant influence of both local and landscape factors on P. citri population density. However, it was found that the variation in mite population density was significantly influenced by citrus plant canopy density. These findings suggest that local factors, particularly the canopy microhabitat conditions, are more critical in affecting the P. citri population compared to landscape factors. Therefore, the management strategy for citrus red mite should be focused on the local, on-farm scale.
The Effect of Citrus Farming Practices on Huanglongbing (HLB) Disease Severity in Sambas, West Kalimantan Zuhran, M.; Mudjiono, Gatot; Puspitarini, Retno Dyah
Jurnal Hortikultura Indonesia (JHI) Vol. 12 No. 2 (2021): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.12.2.108-116

Abstract

Beberapa praktik budidaya dilaporkan mempengaruhi kesesuaian agroekosistem bagi perkembangan penyakit tanaman. Namun demikian, praktik-praktik budidaya jeruk yang mempengaruhi perkembangan penyakit huanglongbing (HLB) dan serangga vektornya yaitu kutu loncat jeruk (KLJ) Diaphorina citri belum banyak diketahui. Penelitian ini mempelajari pengaruh praktik budidaya jeruk terhadap intensitas penyakit HLB melalui survei singkat selama dua minggu pada 37 kebun jeruk yang berlokasi di Kabupaten Sambas. Parameter pengamatan adalah intensitas serangan penyakit HLB, kelimpahan KLJ, dan praktik budidaya yang diterapkan. Pengaruh praktik budidaya terhadap intensitas serangan penyakit HLB dianalisis melalui structural equation modeling berbasis partial least squares (SEM-PLS). Hasil penelitian menunjukkan tingkat penggunaan pestisida, kualitas saluran drainase, dan kepadatan tanaman jeruk berpengaruh positif terhadap intensitas serangan penyakit HLB, sedangkan tingkat keanekaragaman vegetasi dan pemberian pupuk kimia berpengaruh negatif terhadap intensitas serangan penyakit HLB. Pengendalian gulma, kualitas tanah, dan pemangkasan pemeliharaan tidak mempengaruhi intensitas HLB. Oleh karena itu, penanaman jeruk sebaiknya menerapkan sistem polikultur, menggunakan beragam varietas, dan jarak tanam tidak terlalu rapat. Pemberian hara yang dapat meningkatkan ketahanan tanaman jeruk terhadap serangan KLJ hendaknya ditingkatkan. Pengendalian hama dan penyakit tanaman disarankan dilakukan secara terpadu sehingga insektisida hanya digunakan ketika populasi hama mencapai ambang ekonomi. Kata kunci: huanglongbing, jeruk, kutu loncat jeruk, praktik budidaya
Comparison of Five Legumes as Host of Tetranychus sp. (Acari: Tetranychidae) Mass Rearing Puspitarini, Retno Dyah; Qomariyah, Nurul; Afandhi, Aminudin
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.70

Abstract

Two spotted mite (TSM) Tetranychus sp. is often used as host for predator mite Phytoseiidae mass rearing. To obtain high population of TSM in short time, it must be reared in host plants that support optimum growth and development. The objective of this research was to observe population development of TSM on legumes such as mungbean (Vigna angularis), adzukibean (V. radiata), snapbean (Phaseolus vulgaris) and soybean (Glycine max) (wilis and anjasmoro cultivars) and to compare the growth of the legumes based on their fresh leaf weight and leaf area, and cost seed index (CSI). The results showed that mungbean was suitable host plant for TSM mass rearing because its population on this legume was the highest. Mungbean’s fresh leaf weight and leaf area was the highest. If TSM is reared in plant in greenhouse, CSI will become one of criteria for determining the host plant. Although CSI of mungbean was low because TSM population that was reared on it is the highest, the mungbean was the most suitable host plant for TSM growth and development. Another host plant that was suitable for TSM reared was adzukibean.Keywords: legumes, mass rearing, Tetranychus uritcae
Populasi Elaeidobius kamerunicus Faust (Coleoptera: Curculionidae) pada beberapa umur tanaman kelapa sawit Rahardjo, Bambang Tri; Rizali, Akhmad; Utami, Ika Putri; Karindah, Sri; Puspitarini, Retno Dyah; Sahari, Bandung
Jurnal Entomologi Indonesia Vol 15 No 1 (2018): Maret
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.541 KB) | DOI: 10.5994/jei.15.1.31

Abstract

Elaeidobius kamerunicus Faust is an oil palm pollinator insect that lives on the male inflorescence and visits female inflorescence to pollinate due to attracted by its volatile compound. The number of fruit sets of oil palm relates to the population of E. kamerunicus on a plantation. The objective of this study was to investigate the effect of age of oil palm on the population of E. kamerunicus. Field research was conducted in oil palm plantations that located in Pangkalan Lada, Kotawaringin Barat District, in Central Kalimantan. The research method was by measurement of E. camerunicus population on male inflorescences and set up the sticky trap on female inflorescences. Plot with size 7000 m2 (100 trees) was selected on several ages of oil palm i.e. 6, 10 and 16 years. Each plot, the number of male and female inflorescences was counted and some inflorescences were chosen for measurement of E. camerunicus population that was conducted monthly for three months of observation. The results showed that the age of the oil palm affected the population of E. kamerunicus in male inflorescences but not in female inflorescences. The older age of oil palm, the population of E. kamerunicus in male flowers was increasing. The sex ratio of E. kamerunicus found in male and female inflorescences of oil palms tend to female bias. Based on the comparison of E. kamerunicus population between male and female inflorescences on each plot, it was found that visitation value of E. kamerunicus was highest in the young oil palm. In conclusion, increasing age of oil palm trees affected on increasing of E. kamerunicus population in male inflorescences, but visitation value on female inflorescences prone to decline.