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Journal : International Journal of Advanced Multidisciplinary

Evaluation of Molecular Markers and Morphology In Identification of Local Porang Strain in Karawang District Komariah, Ai; Romiyadi; Dyah Setya Wuryani; Luthful Hakim; Nunung Sondari; Lia Amalia; Muhammad Subandi
International Journal of Advanced Multidisciplinary Vol. 2 No. 3 (2023): International Journal of Advanced Multidisciplinary (October - December 2023)
Publisher : Green Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/ijam.v2i3.402

Abstract

Porang plant tubers have the potential to have high economic value, because they contain glucomannan which is good for health and can be processed into a food source to replace carbohydrates. The aim of the study was to identify Karawang Local Porang strains using molecular markers and morphological characters as an effort to conserve and utilize Karawang's local biological resources. Exploration of Local Porang Karawang was carried out at the foot of Mount Sanggabuana which is the habitat of Porang in Karawang district. Molecular identification was carried out at BBPP Biotechnology and Agricultural Genetic Resources with comparisons of Porang Sidrat., Madiun 1 and SS1 Bulukumba varieties. Morphological identification was carried out at the Basic Laboratory of the Faculty of Agriculture, Winaya Mukti University. Based on the results of the DNA amplification of the Porang samples using four RAPD primers, it was found that the three primers showed differences in banding patterns between the Porang Karawang sample and the three Porang varieties for comparison (Porang Madiun 1 Sidrap and SS1 Bulukumba). This shows that there is genetic variation between Porang from Karawang and three other Porang. The results of genetic relationship analysis using four RAPD primers showed that the Porang Karawang sample was different from the Porang samples from the other two areas. The morphological identification results showed differences in characteristics with the comparison. The Karawang Local Porang variety has high quality as indicated by high glucomannan levels of 79.80%.
Pb Heavy Metal Content, Growth and Yield of Four Kale Varieties (Ipomea reptans Poir.) due to Dosage of Chicken Manure with Sediment Media of Sewers Lia Amalia; Nunung Sondari; Budiasih; Tien Turmuktini; Elly Roosma Ria; Kovertina Rakhmi Indriana; Beni Komara
International Journal of Advanced Multidisciplinary Vol. 2 No. 4 (2024): International Journal of Advanced Multidisciplinary (January-March 2024)
Publisher : Green Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/ijam.v2i4.429

Abstract

Food insecurity is a condition in an area, community or household where the level of security and availability does not meet the standards needed for the growth and health of the majority of the population. Kangkung is a heavy metal-absorbing weed plant that many people like as a vegetable. Food security in urban communities is limited by existing land and growing media, so using media from sewer sediment is an alternative. The experiment used a factorial Randomized Block Design (RAK), consisting of two factors, namely the dose of chicken manure (A) with 4 levels as follows: a0 : 0 t ha-1 (control), a1 : 10 t ha-1, a2 : 20 t ha-1 and a3 : 30 t ha-1 and Kangkung Variety (K) with 4 levels as follows: k0 : Serimpi Variety, k1 : Amanda Variety, k2 : Bangkok LP-1 Variety and k3 : Bika Variety, so there are 16 treatment combinations repeated 2 times. The research findings indicated that there was no significant correlation between the dosage of chicken manure and the plant type in terms of plant growth, yield, and absorption of PB heavy metals, with the exception of leaf count. The optimum dose of chicken manure is 5.05 t ha-1 in the Serimpi variety with a maximum number of leaves of 11.30 plants-1, 5.27 t ha-1 in the Amanda variety produces a maximum number of leaves of 16.68 plants-1 , 9.20 t ha-1 in the Bangkok LP-1 variety produces a maximum number of leaves of 16.77 plants-1 and 3.83 t ha-1 in the Bika variety produces a maximum number of leaves of 18.66 plants-1.