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All Journal PROSIDING SEMINAR NASIONAL Biogenesis: Jurnal Ilmiah Biologi Jurnal Kesehatan Masyarakat Andalas (Andalas Journal of Public Health) Prosiding Seminar Biologi Indonesian Journal of Biotechnology Jurnal Teknologi Laboratorium Journal of Health (JoH) Adi Widya : Jurnal Pengabdian Masyarakat Martabe : Jurnal Pengabdian Kepada Masyarakat ABDIMAS SILIWANGI Jurnal Biomedika Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Ilmiah Permas: Jurnal Ilmiah STIKES Kendal Jurnal Nasional Teknologi Terapan Nusantara Science and Technology Proceedings Jurnal Mitra Kesehatan Jurnal Biosense Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) J.Abdimas: Community Health SEMINAR NASIONAL PENELITIAN DAN PENGABDIAN KEPADA MASYARAKAT Jurnal Vokasi Kesehatan Gema Kesehatan Journal Fatmawati Laboratory & Medical Science
Fitri Nadifah
Program Studi D3 Analis Kesehatan STIKES Guna Bangsa Yogyakarta
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Journal : Nusantara Science and Technology Proceedings

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Overabundance of Lactobacillus iners in the Urogenital Microbiome of a Female Patient with Urinary Tract Infection Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/ nstp.2022.2108

Abstract

The microbiome that inhabits the human body plays a role not only in maintaining health but also in causing diseases, such as urinary tract infections (UTIs). This occurs due to changes in the host's body and causes changes in the composition of the microbiota that make up the microbiome. This case report contains the results of 16S rRNA gene sequencing in the urine specimens of a 41-year-old female patient diagnosed with UTI. Illumina MiSeq was carried out for 16S rRNA gene sequencing in the V3-V4 hypervariable region. The 16S rRNA sequence yielded a total of 98,754 high-quality reads with 283 species and 299 operational taxonomic units (OTUs) identified. Based on the sequencing results, we found an overabundance (93%) of Lactobacillus iners as the most abundant and dominant taxa. Lactobacillus iners is one of the bacteria that is difficult to culture. However, with the 16S rNA gene sequencing technology, these bacteria can be detected along with their abundance in the structure of the urogenital microbiome. Bacterial overabundance may have occurred as a result of changes in the host ecology, resulting in UTI clinical symptoms. This result indicates that, in addition to maintaining the health of the urogenital organs, L. iners can also be the causative agent of UTI. An ecological perspective appears to be necessary to determine the composition and function of the microbiota, especially in patients with UTI. The data set has been sent via NCBI as a Sequence Read Archive (SRA).
Overabundance of Lactobacillus iners in the Urogenital Microbiome of a Female Patient with Urinary Tract Infection Fitri Nadifah; Wayan Tunas Artama; Budi Setiadi Daryono; Endah Retnaningrum
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/ nstp.2022.2108

Abstract

The microbiome that inhabits the human body plays a role not only in maintaining health but also in causing diseases, such as urinary tract infections (UTIs). This occurs due to changes in the host's body and causes changes in the composition of the microbiota that make up the microbiome. This case report contains the results of 16S rRNA gene sequencing in the urine specimens of a 41-year-old female patient diagnosed with UTI. Illumina MiSeq was carried out for 16S rRNA gene sequencing in the V3-V4 hypervariable region. The 16S rRNA sequence yielded a total of 98,754 high-quality reads with 283 species and 299 operational taxonomic units (OTUs) identified. Based on the sequencing results, we found an overabundance (93%) of Lactobacillus iners as the most abundant and dominant taxa. Lactobacillus iners is one of the bacteria that is difficult to culture. However, with the 16S rNA gene sequencing technology, these bacteria can be detected along with their abundance in the structure of the urogenital microbiome. Bacterial overabundance may have occurred as a result of changes in the host ecology, resulting in UTI clinical symptoms. This result indicates that, in addition to maintaining the health of the urogenital organs, L. iners can also be the causative agent of UTI. An ecological perspective appears to be necessary to determine the composition and function of the microbiota, especially in patients with UTI. The data set has been sent via NCBI as a Sequence Read Archive (SRA).
Co-Authors Anggraeni Anwarudin, Anwarudin Arum Nuryati Bayu Nur Khotib Bayu Nur Khotib, Bayu Nur Bolosan, Fabio Andre Budi Setiadi Daryono BUDI SETIADI DARYONO Budi Setiadi Daryono Bulu, Martinus Tagu Clara Pattirane, Evelin Dani Setiawan Dapa Ole, Ericson Umbu Robaka De Jesus, Maria Carolina Desto Arisandi Dewi Rahadian, Titah Diah Irawati Dian Wuri Astuti Dian Wuri Astuti, Dian Dieta Dieon Saputri Endah Retnaningrum Endah Retnaningrum Endah Retnaningrum Famela Adelena, Fitria Farida Muhajir, Nurlaili Farida Muhajir, Nurlaili Fitri Nadifah Fitri Retnoningsih Fitriani, Audiah Fuadah Hidayat, Tsamrotul Ika Susilowati Indah Yuliana Barekola Lamablawa Indah Yuliana Barekola Lamablawa, Indah Yuliana Barekola Islami Burhanudin Khatrina, Syahdapuan Dara Lisa Susanti Lisa Susanti, Lisa Liza Novia Lani Maloko, Seprianto Maria D. Owa Lobo Maria Yasintha Bhoga Marthaning Tyas, Wina May Tri Mulyani Melani Lesmono, Inez Merry Elisabeth Mosa, Rambu Pedi Nindhea Ferliza, Yoan Nono, Arianto Nurlaili Farida Muhajir Nurlaili Farida Muhajir, Nurlaili Farida Ohoiwutun, Clara Shinta D.P Puspita, Elisabet Della Reni Aqwil Masithah Retnoningsih, Fitri Sanusi , Kristi Elisabeth Saraswati, Farikhatun Nisaa Sawitri, Nining Eka Siti Fatimah Siti Mahmuda Sofvirah R. Sohy Sofvirah R. Sohy, Sofvirah R. Sri Murtiningrum, Sri SRI RAHAYU Susliyanti, Maria Trisno Agung Wibowo Urfiyah Lisa Azizah, Urfiyah Lisa Velisshia, Velisshia Vixa Meindara Wayan Tunas Artama Wayan Tunas Artama Wayan Tunas Artama Yola Ramadhani Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih Yuliana Prasetyaningsih