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All Journal HAYATI Journal of Biosciences Biogenesis: Jurnal Ilmiah Biologi Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati Makara Journal of Science Journal of Biotechnology and Natural Science Molekul: Jurnal Ilmiah Kimia Berkala Ilmiah Biologi
Dwi Umi Siswanti
Faculty Of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Journal : Makara Journal of Science

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The Effect of Acidic pH on Growth Kinetics, Biomass Productivity, and Prima-ry Metabolite Contents of Euglena sp. Nurafifah, Istini; Hardianto, Muhammad Andhi; Erfianti, Tia; Amelia, Ria; Maghfiroh, Khusnul Qonita; Kurnianto, Dedy; Siswanti, Dwi Umi; Sadewo, Brilian Ryan; Putri, Renata Adaranyssa Egistha; Suyono, Eko Agus
Makara Journal of Science Vol. 27, No. 2
Publisher : UI Scholars Hub

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Euglena is a microalga with the potential to be an environmentally friendly renewable energy resource. The pH value is a crucial factor in micro-algal cultivation. Changes in pH affect the growth and development of microalgae, including the production of biomass and primary metabolites, such as proteins, carbohydrates, and lipids. In this study, Euglena sp. was grown on Cramer-Myers medium and subjected to various acidic conditions. This study aimed to determine the effect of pH on the growth kinetics, biomass, carbohydrate, lipid, and protein contents of Euglena sp. The Euglena sp. culture was optimized at various pH values of 2.5, 3.5, and 4.5. The results were analyzed by one-way analysis of variance at a 95% confidence level, followed by Duncan’s multiple range test. As results, Euglena sp. had the best growth rate, the greatest biomass, and the highest carbohydrate, protein, and lipid contents at pH 3.5 compared to the other pH conditions. The average biomass in the pH 3.5 treatment was 1.600 ± 0.229 g/L, and the carbohydrate, protein, and lipid contents were 5.983 ± 0.056 g/L, 0.196 ± 0.023 µg/mL, and 0.300 ± 0.020 g/L, respectively.
Effect of Mercury Dose Variation on Growth and Nitrate Reductase Activity in Aquarius palifolius (Nees & Mart.) Christenh. & Byng Nurhanifah, Tsurayya; Siswanti, Dwi Umi
Makara Journal of Science Vol. 29, No. 2
Publisher : UI Scholars Hub

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Mercury, a byproduct of mining, can harm the environment. One method to reduce this impact is through phytoremediation. This study used the Mexican sword (Aquarius palifolius), a plant with the potential to absorb and accumulate mercury. The aim of this study was to examine the effect of mercury stress on nitrate reductase activity, which plays a role in plant productivity, and to assess its impact on growth parameters, including plant height growth rate, leaf length, and number of leaves. Three-month-old A. palifolius plants obtained from rice fields in Sleman were treated with mercury at doses of 0, 14, 16, 18, and 20 ppm. The experiment was conducted in triplicate. Nitrate reductase activity was measured using a spectrophotometer, and growth rate was observed over 15 days. Data were analyzed using One-way analysis of variance (ANOVA) and evaluated using Duncan’s Multiple Range Test (DMRT). Mercury exposure led to adaptations, including a 9.5%–13.5% change in nitrate reductase activity and a 50%–65% reduction in the number of leaves. Growth rates of plant height and number of leaves showed no significant differences, even under mercury stress. These results indicate that A. palifolius can tolerate mercury concentrations of 14–20 ppm and has the potential to functions as a phytoremediation agent.
Co-Authors Afifah, Marshanda Nur Roosyana Aini, Hasna Nur Akrima Syahidah Akrima Syahidah Alam, Lucky Prabowo Miftachul Amelia, Ria Ananmaimuna, Jovika Syifa Anindita Della Rosa Riyadi Aprida Niken Arianda Poetri Shofia Rochman Azkiya, Nala Azzizah, Rikka Nur BUDI SETIADI DARYONO Cici Darsih Dea Hastaning Tantri Dedy Kurnianto Diah Rachmawati Eko Agus Suyono Eko Agus Suyono Erfianti, Tia Fitria Yulia Rachmawati Hamdian Hamdian Hardianto, Muhammad Andhi Hari Purwanto Hasna Fatin Affifah Hasna Nur Aini Himawan Tri Bayu Murti Petrus Khusnul Qonita Maghfiroh Kurnianto, Dedy Lena Mardiana Lestari, Melinda Fajar Lestari, Nurrisma Ika Maghfiroh, Khusnul Qonita Malika, Pradhika Cikal Marlyn Dian Laksitorini, Marlyn Dian Marshanda Nur Roosyana Afifah Maryani Maryani Melinda Fajar Lestari Melinda Fajar Lestari Mifta Arlinda Nadhifa Athaya Khairunnisa Niken Wulansari Nilamsari, Regina Nindy Senissia Asri Nur Hidayah Pangestuti Nurafifah, Istini Nurhanifah, Tsurayya Okky Shavira Riesty Palupi, Dwi Rekno Putri, Renata Adaranyssa Egistha Rahmasari, Firdaus Salvia Rain, Oryza Enwiera Rega Virgiyana Agustin Rega Virgiyana Agustin Rega Virgiyana Agustin Ria Amelia Riyadi, Anindita Della Rosa Sadewo, Brilian Ryan Sahid, Muhammad Novrizal Abdi Sinambela, Benn Gerson Juan Moreno Soenarwan Hery Poerwanto, Soenarwan Hery Solly Aryza Sudjino Sudjino Tantri, Dea Hastaning Tia Erfianti Untari, Ludmilla Fitri Utami, Indrawati Dian Utaminingsih Utaminingsih Vidhantyka, K.H. Mahadhevy Ryrathna Wardana, Wisnu Eka Wisnu Eka Wardana