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Daya Hambat Jamur Endofitik Terhadap Fusarium oxysporum f.sp. capsici Penyabab Layu Fusarium Pada Tanaman Cabai (Capsicum frutescens L.) Polhaupessy, Vanda C; Taribuka, Johanna; Talahaturuson, Abraham
Jurnal Agrosilvopasture-Tech Vol 4 No 1 (2025): Jurnal Agrosilvopasture-Tech
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/j.agrosilvopasture-tech.2025.4.1.100

Abstract

This research aims to obtain endophytic fungi from healthy chili plants (Capsicum frutescens L.) and test the inhibitory power of endophytic fungi, which are thought to have the potential to control the pathogen that causes fusarium wilt disease (Fusarium oxysporum f.sp. capsici) in chili plants (Capsicum frutescens L.). The exploration results obtained five genera of endophytic fungi from different plant parts and in different villages and hamlets, including Colletotrichum sp.TWtc, Rhizoconia sp.TWdc, Trichoderma sp.TKac, Penicillium sp.TKbc, and Aspergilus sp.TObtc, The percentage of inhibitory power of the five endophytic fungal genera against the pathogenic fungus F.oxysporum f.sp capsici is as follows: Trichoderma sp.TKac, which is 85.29%, followed by the isolate Rhizoctonia sp.TWdc, which is 62.14%, isolates Penicillium sp. TKbc was 61.31%, Colletotrichum sp.TWtc isolate was 60.27% and the lowest inhibitory power was Aspergillus sp.TObtc isolates were 46.42%. Conclusion: The endophytic fungus Trichoderma sp. TKac has the highest inhibitory ability against the pathogenic fungus F.oxysporum f.sp.capsici, so it can be used as a biological control agent.
Potensi Minyak Atsiri Kayu Manis dan Kayu Lawang Sebagai Pengendali Hayati Fusarium oxysporum Hariroh, Siti Harir; Marzuki, Ilyas; Leiwakabessy, Christoffol; Talahaturuson, Abraham
JURNAL PERTANIAN KEPULAUAN Vol 9 No 2 (2025): Jurnal Pertanian Kepulauan
Publisher : Fakultas Pertanian, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jpk.2025.9.2.72x

Abstract

This research aims to analyze the potential of essential compounds of Cinnamonum burmannii and Cinnamonum cullilawan as a bioagent for Fusarium oxysporum and to test the efficacy of both essential oils in controlling the growth of the fungus F. oxysporum. Oil extraction was carried out using the steam-water distillation method, and analysis of the components of the oil compounds of the cinnamon was carried out using the GC-MS method. Essential oils were tested for their efficacy for inhibiting F. oxysporum with four concentrations, namely 0.06%, 0.125%, 0.25%, and 0.5% on PDA and PDB media. The results show that the main essential compound in C. burmannii is cinnamaldehyde at 20.61%, and in C. cullilawan, it is eugenol at 26.33%. The essential compounds of both have antifungal activity against F. oxysporum, with a concentration rate of 0.125% being able to inhibit the growth of F. oxysporum. The main active compounds, such as cinnamaldehyde and eugenol, play an important role in the fungus control mechanism. This research concludes that the essential compounds from both have the potential to inhibit F. oxysporum growth.
Antagonisme In-Vitro Lima Isolat Lokal Trichoderma spp. Asal Rhizosfer Terhadap Rhizoctonia solani Penyebab Busuk Pelepah Jagung Soplanit, Rainhart C; Patty, Jogeneis; Talahaturuson, Abraham
JURNAL BUDIDAYA PERTANIAN Vol 17 No 2 (2021): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp/2021.17.2.89

Abstract

Environmentally friendly biological control can be an alternative for suppressing the pathogen Rhizoctonia solani, the cause of sheath rot in corn plants. This can be done by utilizing biological agents, such as Trichoderma spp. Research on in vitro antagonism of five local isolates of Trichoderma spp. of rhizospheric origin against R. solani was carried out at the Plant Disease Diagnosis Laboratory, Faculty of Agriculture Unpatti, Ambon. This research aimed to determine the inhibition of five Trichoderma spp isolates against R. solani in vitro and to the mechanism of its inhibition. This study used a Completely Randomized Design to test the five local isolates of Trichoderma spp. The experiment was carried out with 4 replications, and each experimental unit consisted of 2 Petri dishes for testing. The results showed that the five local isolates of Trichoderma spp. from the rhizosphere could suppress the growth of R. solani on corn plants in vitro. On the seventh day of observation, the highest inhibition occurred in Trichoderma isolate from the Banda Baru coconut rhizosphere (TrichoRKlBB), which was 100% and the local Trichoderma isolate rhizosphere of Nuruwe cocoa (TrichoRKN) 100%, and the lowest percentage occurred using the Trichoderma isolate rhizosphere of Waisamu coconut (TrichoRKlW), namely (80.13%). The mechanisms of antagonism that occured were the mechanisms of competition and microparasitism.
Ekspresi Gen PR-1 Melalui Induksi Ketahanan Tanaman Padi terhadap Xanthomonas oryzae pv. oryzae Menggunakan Lysinibacillus sphaericus dan Asam Salisilat Leiwakabessy, Christoffol; Giyanto, Giyanto; Muttaqin, Kikin Hamzah; Trikoesoemaningtyas, Trikoesoemaningtyas; Talahaturuson, Abraham
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1559

Abstract

Xanthomonas oryzae pv.oryzae merupakan salah satu bakteri patogen yang menyerang tanaman padi. Pengendalian terhadap penyakit ini telah banyak dilakukan dan salah satu teknik pengendalian yang dikembangkan saat ini melalui teknologi induksi ketahanan tanaman yang mengekspresikan gen PR-1. Penelitian kami untuk mendeskripsikan ekspresi gen PR-1 pada varietas padi IR64 yang terinduksi resistensinya oleh asosiasi antara bakteri endofit dan asam salisilat. Gen PR-1 dianalisis secara molekuler dan ekspresinya menunjukkan tanaman padi yang terinduksi resistensinya secara tunggal maupun kombinasi oleh kedua agens ini mem­perlihatkan adanya ekspresi protein PR-1. Ekspresi protein ini dipicu oleh aktivitas kedua agens penginduksi yang bekerja secara simultan untuk membentuk protein yang mampu menekan perkembangan penyakit ini. PR-1 bekerja sesuai dengan mekanisme dari masing-masing agens penginduksi resistensi tanaman. Varietas IR64 yang diketahui rentan terhadap penyakit hawar daun bakteri terbukti dapat ditingkatkan ketahanannya menjadi moderat.  Pemanfaatan induksi ketahanan tanaman melalui aktivitas kedua agens pengimbas yang membentuk protein PR-1 perlu dilanjutkan dengan inovasi formulasi teknik pengendalian yang diberikan secara simultan sebagai alternatif strategi pengendalian hawar daun bakteri maupun penyakit lainnya ke depan dengan pendekatan ramah lingkungan dan berkelanjutan.
SOSIALISASI DAN EDUKASI PENGEMBANGAN AGROEKOWISATA LECI DI NEGERI NAKU KECAMATAN LEITIMUR SELATAN KOTA AMBON Hukom, Zakarias Frans Mores; Mardiatmoko, Gun; Kunu, Peiter J.; Talahaturuson, Abraham; Effendy, Jollanda; Taihuttu, Hermina N.
MAANU: Jurnal Pengabdian Kepada Masyarakat Vol 3 No 2 (2025): Maanu Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/maanuv3i2p104-114

Abstract

The lychee tree (Litchi chinensis Sonn.) is perennial fruit plant cultivated in yards and gardens on a small or large scale for the development of agro-ecotourism, agribusiness, and fruit agro-industry. In Naku village, lychee tree havenot been cultivated commercially, so the productivity and quality of the fruit are still relatively low. Developing lychee plantations in Naku for commercial purposes as part of an agro-ecotourism package requires a thorough understanding of the sustainable application of appropriate agro-eco-technology. Therefore, increasing the productivity and quality of lychee fruit in Naku requires a program to raise awareness of the potential for lychee agro-ecotourism development. The lychee agro-ecotourism technology package that was socialized in the Community Service activities in Naku village includes material on the potential for developing lychee agro-ecotourism, the function and benefits of lychee trees as agro-ecotourism landscape plants, land conservation and irrigation techniques for lychee, characterization of the Amboneinsis lychee variety and protection. It is hoped that the technology transfer in this socialization activity can be useful in motivating the people of Naku village towards the potential of developing lychee fruit agro-ecotourism which has high economic value and future business opportunities
Penilaian Efektivitas Metabolit Sekunder Trichoderma harzianum dalam Menghambat Pertumbuhan Colletotricum capsici dan Cercospora capsici : Kajian In Vitro Baida Watngil; A. Marthin Kalay; Abraham Talahaturuson; Costanza Uruilal
AGROLOGIA: Jurnal Ilmu Budidaya Tanaman Vol 13 No 1 (2024): Agrologia : Jurnal Ilmu Budidaya Tanaman
Publisher : Fakultas Pertanian, Universitas Pattimura Ambon, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/agrologia.v13i1.12635

Abstract

The cultivation of chili plants is often faced with obstacles such as disease attacks, especially anthracnose disease caused by the fungus Colletotricum capsici and leaf spot disease caused by the fungus Cercospora capsici. This study aimed to determine the effective concentration of T. harzianum secondary metabolites (MSTh) in inhibiting the growth of Colletotricum capsici and Cercospora capsici in vitro. The experiments used various MSTh concentrations: 0%, 10%, 20%, 30%, 40%, and 50%. The research results found that administration of MSTh could inhibit the growth of Colletotricum capsici and Cercospora capsici in vitro. Giving MSTh at a concentration of 10% can significantly inhibit the growth of Colletotricum capsici and Cercospora capsici compared to without MSTh. The effective concentration indicated by the percentage value of inhibitory power and colony weight reduction is an MSTh concentration of 40% with a growth inhibition of Colletotricum capsici of 89.49% and an MSTH concentration of 50% with a growth inhibition of Cercospora capsici of 57.78%.
Eksplorasi Jamur Antagonis Pada Rizosfer Tanaman Kelapa (Cocos nucifera L.) Di Pulau Ambon Andi Ubaidillah; Jogeneis Patty; Abraham Talahaturuson
AGROLOGIA: Jurnal Ilmu Budidaya Tanaman Vol 13 No 2 (2024): Agrologia : Jurnal Ilmu Budidaya Tanaman
Publisher : Fakultas Pertanian, Universitas Pattimura Ambon, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/agrologia.v13i2.14566

Abstract

Pengendalian hayati merupakan suatu pemanfaatan mikroorganisme yang bertujuan untuk mengendalikan Organisme Pengganggu Tanaman (OPT). Adapun penelitian ini bertujuan untuk mendapatkan identifikasi jamur antagonis serta menguji daya hambat jamur tersebut terhadap Phytopthora palmivora. Penelitian dilaksanakan di lahan kebun kelapa milik petani di pulau Ambon dan di laboratorium Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan (BBPPTP) Ambon, pada bulan Juni – Oktober 2023. Mengggunakan metode eksplorasi dan eksperimen di Laboratorium BBPPTP Ambon untuk uji antagonisme. Bahan yang digunakan yaitu tanah disekitar perakaran tanaman kelapa yang sehat. Pengambilan sampel dilakukan dengan metode Simple Random Sampling dan menetapkan 4 lokasi di Pulau Ambon. Kemudian sampel tanah dibawa ke laboratorium untuk dilakukan isolasi dan identifikasi serta uji antagonisme. Hasil eksplorasi jamur antagonis rhizosfer tanaman kelapa di pulau Ambon diperoleh empat genus jamur antagonis yaitu Trichoderma, Aspergillus, Rhizopus dan Mucor. Perkembangan penghambatan patogen Phytoptora palmivora oleh isolat Trichoderma menunjukkan bahwa masing-masing isolat tersebut memiliki daya antagonisme. Berdasarkan tingkatan kelas antagonismenya, isolat TrichoRKSW1, TrichoRKSW2, TrichoRKSW4, TrichoRKSW5, TrichoRKLH2, TrichoRKLH4, TrichoRKLH5, TrichoRKNL1, TrichoRKNL3, TrichoRKNL4 dan TrichoRKLBH3 dapat dikategorikan kedalam tingkat antagonisme kelas 1, sedangkan isolat TrichoRKNL2, TrichoRKLBH1, TrichoRKLBH4 dan TrichoRKLBH5 dapat dikategorikan kedalam tingkat antagonisme kelas 2. Isolat Aspergillus, Mucor dan Rhizopus termasuk kedalam tingkat kelas 3.
Karakteristik Tanah dan Status Kesuburan Lahan Ladang Berpindah di Kabupaten Maybrat Provinsi Papua Barat Daya Niko Kareth; Rafael M. Osok; A. Marthin Kalay; Abraham Talahaturuson
AGROLOGIA: Jurnal Ilmu Budidaya Tanaman Vol 14 No 1 (2025): Agrologia : Jurnal Ilmu Budidaya Tanaman
Publisher : Fakultas Pertanian, Universitas Pattimura Ambon, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/agrologia.v14i1.18675

Abstract

Sistem perladangan berpindah menunjukkan beberapa kesamaan, namun juga memiliki variasi tergantung pada kondisi biofisik wilayah dan budaya masyarakat, terutama dalam aspek sosial dan ekonomi yang memengaruhi pola penggunaan lahan. Penelitian ini bertujuan untuk mengkaji (1) karakteristik lahan ladang berpindah, (2) status kesuburan tanah, dan (3) arahan pemanfaatan lahan ladang berpindah untuk pengembangan tanaman pangan lokal di Kabupaten Maybrat. Penelitian dilakukan pada bulan Mei 2024 di lahan ladang berpindah Kampung Huberita, Distrik Ayamaru Timur. Variabel yang diamati meliputi karakteristik lahan (jenis tanah, geologi, topografi, penggunaan lahan, sistem tanam, jenis komoditas), sifat fisik tanah (kadar air, bobot isi, berat jenis partikel, porositas), sifat kimia tanah (pH, KTK, KB, C-organik, N-total, P₂O₅, K₂O, dan basa-basa seperti Ca, Mg, Na, K), Hasil menunjukkan bahwa praktik tebang-bakar memengaruhi sifat fisik, kimia, dan biologi tanah. Kadar air dan bobot isi tanah meningkat pada lahan berumur 3 minggu dan 3 bulan, namun permeabilitas tanah menurun akibat rendahnya kandungan bahan organik. pH tanah meningkat, sementara C-organik menurun pada umur 3 bulan. K dan P tersedia sangat tinggi, terutama di Ayamaru. KTK meningkat seiring naiknya kadar Ca dan Mg. Secara keseluruhan, kesuburan meningkat sementara waktu, tetapi mulai menurun setelah bulan ketiga karena berkurangnya bahan organik dan unsur hara.