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Daya Hambat Jamur Endofitik Terhadap Fusarium oxysporum f.sp. capsici Penyabab Layu Fusarium Pada Tanaman Cabai (Capsicum frutescens L.) Polhaupessy, Vanda C; Taribuka, Johanna; Talahaturuson, Abraham
Jurnal Agrosilvopasture-Tech Vol 4 No 1 (2025): Jurnal Agrosilvopasture-Tech
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/j.agrosilvopasture-tech.2025.4.1.100

Abstract

This research aims to obtain endophytic fungi from healthy chili plants (Capsicum frutescens L.) and test the inhibitory power of endophytic fungi, which are thought to have the potential to control the pathogen that causes fusarium wilt disease (Fusarium oxysporum f.sp. capsici) in chili plants (Capsicum frutescens L.). The exploration results obtained five genera of endophytic fungi from different plant parts and in different villages and hamlets, including Colletotrichum sp.TWtc, Rhizoconia sp.TWdc, Trichoderma sp.TKac, Penicillium sp.TKbc, and Aspergilus sp.TObtc, The percentage of inhibitory power of the five endophytic fungal genera against the pathogenic fungus F.oxysporum f.sp capsici is as follows: Trichoderma sp.TKac, which is 85.29%, followed by the isolate Rhizoctonia sp.TWdc, which is 62.14%, isolates Penicillium sp. TKbc was 61.31%, Colletotrichum sp.TWtc isolate was 60.27% and the lowest inhibitory power was Aspergillus sp.TObtc isolates were 46.42%. Conclusion: The endophytic fungus Trichoderma sp. TKac has the highest inhibitory ability against the pathogenic fungus F.oxysporum f.sp.capsici, so it can be used as a biological control agent.
Potensi Minyak Atsiri Kayu Manis dan Kayu Lawang Sebagai Pengendali Hayati Fusarium oxysporum Hariroh, Siti Harir; Marzuki, Ilyas; Leiwakabessy, Christoffol; Talahaturuson, Abraham
JURNAL PERTANIAN KEPULAUAN Vol 9 No 2 (2025): Jurnal Pertanian Kepulauan
Publisher : Fakultas Pertanian, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jpk.2025.9.2.72x

Abstract

This research aims to analyze the potential of essential compounds of Cinnamonum burmannii and Cinnamonum cullilawan as a bioagent for Fusarium oxysporum and to test the efficacy of both essential oils in controlling the growth of the fungus F. oxysporum. Oil extraction was carried out using the steam-water distillation method, and analysis of the components of the oil compounds of the cinnamon was carried out using the GC-MS method. Essential oils were tested for their efficacy for inhibiting F. oxysporum with four concentrations, namely 0.06%, 0.125%, 0.25%, and 0.5% on PDA and PDB media. The results show that the main essential compound in C. burmannii is cinnamaldehyde at 20.61%, and in C. cullilawan, it is eugenol at 26.33%. The essential compounds of both have antifungal activity against F. oxysporum, with a concentration rate of 0.125% being able to inhibit the growth of F. oxysporum. The main active compounds, such as cinnamaldehyde and eugenol, play an important role in the fungus control mechanism. This research concludes that the essential compounds from both have the potential to inhibit F. oxysporum growth.
Antagonisme In-Vitro Lima Isolat Lokal Trichoderma spp. Asal Rhizosfer Terhadap Rhizoctonia solani Penyebab Busuk Pelepah Jagung Soplanit, Rainhart C; Patty, Jogeneis; Talahaturuson, Abraham
JURNAL BUDIDAYA PERTANIAN Vol 17 No 2 (2021): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp/2021.17.2.89

Abstract

Environmentally friendly biological control can be an alternative for suppressing the pathogen Rhizoctonia solani, the cause of sheath rot in corn plants. This can be done by utilizing biological agents, such as Trichoderma spp. Research on in vitro antagonism of five local isolates of Trichoderma spp. of rhizospheric origin against R. solani was carried out at the Plant Disease Diagnosis Laboratory, Faculty of Agriculture Unpatti, Ambon. This research aimed to determine the inhibition of five Trichoderma spp isolates against R. solani in vitro and to the mechanism of its inhibition. This study used a Completely Randomized Design to test the five local isolates of Trichoderma spp. The experiment was carried out with 4 replications, and each experimental unit consisted of 2 Petri dishes for testing. The results showed that the five local isolates of Trichoderma spp. from the rhizosphere could suppress the growth of R. solani on corn plants in vitro. On the seventh day of observation, the highest inhibition occurred in Trichoderma isolate from the Banda Baru coconut rhizosphere (TrichoRKlBB), which was 100% and the local Trichoderma isolate rhizosphere of Nuruwe cocoa (TrichoRKN) 100%, and the lowest percentage occurred using the Trichoderma isolate rhizosphere of Waisamu coconut (TrichoRKlW), namely (80.13%). The mechanisms of antagonism that occured were the mechanisms of competition and microparasitism.
Ekspresi Gen PR-1 Melalui Induksi Ketahanan Tanaman Padi terhadap Xanthomonas oryzae pv. oryzae Menggunakan Lysinibacillus sphaericus dan Asam Salisilat Leiwakabessy, Christoffol; Giyanto, Giyanto; Muttaqin, Kikin Hamzah; Trikoesoemaningtyas, Trikoesoemaningtyas; Talahaturuson, Abraham
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1559

Abstract

Xanthomonas oryzae pv.oryzae merupakan salah satu bakteri patogen yang menyerang tanaman padi. Pengendalian terhadap penyakit ini telah banyak dilakukan dan salah satu teknik pengendalian yang dikembangkan saat ini melalui teknologi induksi ketahanan tanaman yang mengekspresikan gen PR-1. Penelitian kami untuk mendeskripsikan ekspresi gen PR-1 pada varietas padi IR64 yang terinduksi resistensinya oleh asosiasi antara bakteri endofit dan asam salisilat. Gen PR-1 dianalisis secara molekuler dan ekspresinya menunjukkan tanaman padi yang terinduksi resistensinya secara tunggal maupun kombinasi oleh kedua agens ini mem­perlihatkan adanya ekspresi protein PR-1. Ekspresi protein ini dipicu oleh aktivitas kedua agens penginduksi yang bekerja secara simultan untuk membentuk protein yang mampu menekan perkembangan penyakit ini. PR-1 bekerja sesuai dengan mekanisme dari masing-masing agens penginduksi resistensi tanaman. Varietas IR64 yang diketahui rentan terhadap penyakit hawar daun bakteri terbukti dapat ditingkatkan ketahanannya menjadi moderat.  Pemanfaatan induksi ketahanan tanaman melalui aktivitas kedua agens pengimbas yang membentuk protein PR-1 perlu dilanjutkan dengan inovasi formulasi teknik pengendalian yang diberikan secara simultan sebagai alternatif strategi pengendalian hawar daun bakteri maupun penyakit lainnya ke depan dengan pendekatan ramah lingkungan dan berkelanjutan.
SOSIALISASI DAN EDUKASI PENGEMBANGAN AGROEKOWISATA LECI DI NEGERI NAKU KECAMATAN LEITIMUR SELATAN KOTA AMBON Hukom, Zakarias Frans Mores; Mardiatmoko, Gun; Kunu, Peiter J.; Talahaturuson, Abraham; Effendy, Jollanda; Taihuttu, Hermina N.
MAANU: Jurnal Pengabdian Kepada Masyarakat Vol 3 No 2 (2025): Maanu Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/maanuv3i2p104-114

Abstract

The lychee tree (Litchi chinensis Sonn.) is perennial fruit plant cultivated in yards and gardens on a small or large scale for the development of agro-ecotourism, agribusiness, and fruit agro-industry. In Naku village, lychee tree havenot been cultivated commercially, so the productivity and quality of the fruit are still relatively low. Developing lychee plantations in Naku for commercial purposes as part of an agro-ecotourism package requires a thorough understanding of the sustainable application of appropriate agro-eco-technology. Therefore, increasing the productivity and quality of lychee fruit in Naku requires a program to raise awareness of the potential for lychee agro-ecotourism development. The lychee agro-ecotourism technology package that was socialized in the Community Service activities in Naku village includes material on the potential for developing lychee agro-ecotourism, the function and benefits of lychee trees as agro-ecotourism landscape plants, land conservation and irrigation techniques for lychee, characterization of the Amboneinsis lychee variety and protection. It is hoped that the technology transfer in this socialization activity can be useful in motivating the people of Naku village towards the potential of developing lychee fruit agro-ecotourism which has high economic value and future business opportunities