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ISOLASI Trichoderma spp. DAN DAYA ANTAGONISMENYA TERHADAP SCLEROTIUM ROLFSII SACC. PENYEBAB PENYAKIT LAYU PADA TANAMAN CABAI (Capsicum anuum) SECARA IN-VITRO Costanza Uruilal; Abraham Talahaturuson; Wihelmina Rumahlewang; Jogeneis Patty
JURNAL BUDIDAYA PERTANIAN Vol 13 No 2 (2017): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2017.13.2.64

Abstract

The objective of this study is to isolation and agonistic test ability of Trichoderma spp. againts Sclerotium rolfsii Sacc. cause of wilting on pepper plants and has been conducted in Pathogenicity Laboratory Faculty of Agriculture Unpatti. The study use 5 treatment of isolate Trichoderma spp. (Tc3, Tc4, Tc5, Tc6 and Tc7) with 3 replications so that there are 15 experimental units. The results showed that the five isolates Trichoderma spp. has an antagonistic power to S. rolfsii with an average percentage of inhibition of S. rolfsii of 26,01%. Percentage of inhibition bolth of isolate ware not significantly different at 95% level test results between treatment. Average percentage inhibition of S. rolfsii by Trichoderma spp. each treatment was Tc6 = 27,31%, Tc3 = 26,63%, Tc5 = 26,05%, Tc7 = 25,69% and Tc4 = 24,37%. Keywords: antagonism, Trichoderma spp., Sclerotium rolfsii Abstrak Penelitian ini bertujuan mengisolasi dan menguji kemampuan antagonis Trichoderma spp. terhadap Sclerotium rolfsii Sacc. penyebab layu pada tanaman cabai dan telah dilaksanakan di Laboratorium Patogenisitas Fakultas Pertanian Unpatti, dengan menggunakan 5 perlakuan isolat Trichoderma spp. (Tc3, Tc4, Tc5, Tc6 dan Tc7) dengan 3 ulangan sehingga terdapat 15 satuan percobaan. Hasil penelitian menunjukkan bahwa kelima isolat Trichoderma sp. mempunyai daya antagonis terhadap S. rolfsii dengan rata-rata persentase penghambatan S. rolfsii sebesar 26%. Hasil analisis varians pada taraf 95% menunjukkan tidak ada perbedaan nyata antara perlakuan. Rata-rata persentase penghambatan S. rolfsii oleh Trichoderma spp. masing-masing perlakuan berturut-turut adalah Tc6 = 27,31%, Tc3 = 26,63%, Tc5 = 26,05%, Tc7 = 25,69% dan Tc4 = 24,37%, dengan rata-rata 26,01%. Kata kunci: antagonisme, Trichoderma spp., Sclerotium rolfsii
Efek Perendaman Benih dengan Pupuk Hayati terhadap Pertumbuhan Bibit dan Serangan Penyakit Rebah Semai pada Tomat (Solanum lycopersicum) Cendy Monica Wohel; A. Marthin Kalay; Abraham Talahaturuson
Jurnal Agroekoteknologi Vol 14, No 1 (2022)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v14i1.15255

Abstract

The use of biofertilizers is part of an environmentally friendly plant cultivation. In order to analyze the effect of soaking seeds with several types of biofertilizers on seedling growth and damping off disease in tomatoes, a greenhouse experiment has been carried out using biofertilizers and the fungicide Propineb as control treatment. The biofertilizers used were mixed biofertilizer (BION-UP), Consortium isolates of tomato rhizosphere bacteria (CITRB), consortium of Azotobacter chrococcum and Trichoderma harzianum (Azoto-Tricho). The results showed that soaking tomato seeds in bioagens solution increased plant height, number of petioles, fresh weight and dry weight of plants, as well as suppressed damping off disease in tomato nursery. The CITRB biofertilizer has a better effect on plant growth and diseases suppression than another biofertilizers and Propineb fungicide. CITRB enable to increase plant height by 49.97%, number of petioles by 60.00%, plant fresh weight by 107.23%, dry weight of plants by 129.41%, and suppress damping off disease by 56.67%. BION-UP, CITRB and Azoto-Tricho were able to suppress damping off disease by 22.22%, 27.78% and 16.67% respectively.  The ability of all biofertilizer to suppress damping off disease incidence were better than Propineb fungicide.
Application of Biofertilizer Consortium of Strain Bacillus sp with Different Concentrations and Aplication Methods on Growth of Nutmeg Seeds (Myristica fragrans Houtt) A. Marthin Kalay; Henry Kesaulya; Abraham Talahaturuson; Herman Rehatta; Reginawanti Hindersah
Agrologia Vol 9, No 1 (2020): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v9i1.1060

Abstract

The growth of nutmeg seeds requires adequate nutrition by mechanism of bacteria activity associated with roots and leaves. The aim of study was to determine the effect of application of bacillus strain consortium biofertilizer on nutmeg seedlings. The experiment was arranged by using completely randomized design with 0% of PBH as control, and 0.15% and 0,30% of PBH concentration by spraying to leaves and by pouring in the soil around the stem, respectivelly. The results showed that the application of 0.15% and 0.30% of PBH concentration has significant effect in plant height, shoot dry weight, dry weight root and plant dry weight, but not at the number of root, root length, stem diameter, and the ratio of shoot and root dry weight of nutmeg seedlings after four month planting in the polybag. The concentration of PBH 0.30% has a greater influence on the nutmeg seed growth, and can be a solution to increasee  seed growth. 
Aplikasi Pupuk Hayati Konsorsium Dan Inokulan Padat Trichoderma harzianum Terhadap Produktivitas Tanaman Sawi Pada Lahan Terkontaminasi Rhizoctonia solani A Marthin Kalay; M Riadh Uluputty; Juniart Leklioy; Reginawanti Hindersah; Abraham Talahaturuson
Agrologia Vol 5, No 2 (2016): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v5i2.185

Abstract

Choy sum (Brassica rapa L.) cultivation main obstacle in Maluku was soil fertility and plant diseases. Biological agent and Biofertilizers will play a significant role to reduce the intensity of disease attacks, and increase crop productivity. The objective of this experiment  was to study the effects of nitrogen fixing and phosphate solubilizing microbe (Bion-UP) and Trichoderma harzianum solid inokulan (Bokelas Plus) on choy sum productivity grown on land Rhizoctonia solani-contaminated land. The experimental treatments  were liquid  biofertilizer and Bokelas Plus which has been combined into 6 treatments: Without biofertilizer and Bokelas Plus, 50 kg Bokelas Plus/ha without biofertilizer, 15 mL biofertilizer per plant without Bokales Plus, 15 mL biofertilizer per plant + 50 Kg Bokelas Plus/ha, 15 mL biofertilizer  per plant + 25 kg Bokelas Plus/ha, and 7.5 mL biofertilizer per plant + 50 kg Bokales Plus/ha. The results showed that giving of Bokelas Plus and biofertilizer with different dose levels decreased  leaf blight intensity and increasing fresh weight of choy sum. Biofertilizer application with and without Bokelas Plus decreased the intensity of leaf blight disease by 39.14% - 46, 93%, and increase the fresh weight of choy sum by 15.19% - 36.25%. 
Efek Aplikasi Pupuk Hayati terhadap Populasi Mikroba dan Ketersediaan Unsur Hara Makro pada Tanah Entisol A. Marthin Kalay; Alexander Sesa; Adelina Siregar; Abraham Talahaturuson
Agrologia Vol 8, No 2 (2019): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v8i2.1011

Abstract

Penggunaan mikroba sebagai pupuk hayati untuk meningkatkan produktivitas tanaman merupakan tindakan budidaya yang efisien, ramah lingkungan dan berkelanjutan. Penelitian ini bertujuan untuk pupuk hayati konsorsium yang berasal dari akar bambu (PHK-AB), akar rumput gajah (PHK-ARG) dan pupuk hayati konsorsium Bion Up (PHK-Bion Up) dengan konsentrasi berbeda terhadap populasi total jamur dan bakteri; dan ketersediaan unsur hara makro pada tanah entisol setelah tanaman sawi.  Perlakuan yang dicobakan adalah tanpa perlakuan (kontrol); aplikasi PHK-Bion UP, PHK-AB dan PHK-ARG masing-masing dengan dengan konsentrasi 1%, 1,5%, dan 2%.  Hasil penelitian ditemukan bahwa pemberian pupuk hayati konsorsium Bion  UP, pupuk hayati asal akar bambu dan pupuk hayati konsorsium asal akar rumput gajah dapat : (1) menurunkan populasi total jamur tetapi meningkatkan populasi total bakteri; (2) pH dan kandungan N-total tanah tidak mengalami perubahan; (3) kandungan P2O5 dan K2O tersedia tanah mengalami peningkatan.  Pnelitian ini menjelaskan bahwa perbedaan konsentrasi antara pupuk hayati tidak memberikan pengaruh terhadap populasi total jamur dan bakteri, pH, kandungan N-total, dan kandungan P2O5 dan K2O tersedia tanah.Kata kunci: akar bambo, akar rumput gajah, biokontrol, pupuk hayati.
Pemberian Pupuk Hayati dengan Interval Waktu Aplikasi Berbeda terhadap Perkembangan Penyakit Rebah Semai dan Pertumbuhan Sawi (Brasica Rapa) di Pesemaian Silvia Kilian; A. Marthin Kalay; Abraham Talahaturuson
Jurnal Agroekoteknologi Vol 14, No 2 (2022)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v14i2.17506

Abstract

Seedling fall disease caused by the fungus Rhizoctonia solani is one of the limiting factors in the cultivation of mustard plants. This pathogen can attack plants in the nursery as well as after the plants in the field. The aim of this study was to determine the effect of using biological fertilizers Azoto-Trico and KIBPF with different application time intervals on the development of seedling fall disease and the growth of mustard in the nursery. The study used Azoto-Tricho and KIBPF biological fertilizers with application intervals of 2 days, 4 days and 6 days. The results showed that the application of biological fertilizers Azoto-Tricho and KIBPF with different application time intervals had an effect on controlling seedling fall disease and increasing plant height and plant fresh weight. The application of Azoto-Tricho biofertilizer and KIBPF if applied at intervals of 2 days or 4 days or 6 days had the same ability to control seedling fall disease and increase plant height and fresh weight of mustard plants in nurseries.
Efek Penggunaan Metabolit Sekunder Trichoderma harzianum terhadap Penyakit Busuk Buah Phomopsis, Hama Perusak Daun Epilachna, dan Hasil Tanaman Terung A. Marthin Kalay; Jeffij Hasinu; Widia Eka Putri; Abraham Talahaturuson
Jurnal Agroekoteknologi Vol 15, No 1 (2023)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v15i1.20003

Abstract

Upaya peningkatan produktivitas terung seringkali terkendala kerusakan tanaman oleh serangan fitopatogen dan hama. Pemanfaatan metabolit sekunder agens hayati untuk pengendalian fitopatogen dan hama perusak pada tanaman, merupakan cara pengendalian yang ramah lingkungan. Penelitian ini bertujuan untuk mendapatkan konsentrasi metabolit sekunder Trichoderma harzianum yang efektif untuk mengendalikan penyakit busuk buah Phomopsis vexans dan hama perusak daun Epilachna, serta untuk meningkatkan produksi terung. Perlakuan yang dicobakan adalah metabolit sekunder T. harzianum dengan konsentrasi 0%, 10%, 20% dan 30%. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder T. harzianum menekan penyakit busuk buah Phomopsis, tetapi tidak berpengaruh terhadap kerusakan daun Epilachna. Pengobatan dengan konsentrasi 10%, 20% dan 30% masing-masing dapat menurunkan intensitas penyakit 51.65%, 64.25% dan 69.63%. Kerusakan daun oleh Epilachna sp tergolong ringan untuk semua tingkat konsentrasi yakni berkisar 3.65-4.32%. Perlakuan metabolit sekunder T. harzianum dengan berbagai tingkat konsentrasi berpengaruh terhadap panjang buah tetapi terhadap jumlah buah, diameter buah, dan berat buah tidak berpengaruh
NITROGEN FIXING BACTERIA AZOTOBACTER AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN Reginawanti Hindersah; Marthin Kalay; Abraham Talahaturuson; Yansen Lakburlawal
Agric Vol. 30 No. 1 (2018)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2018.v30.i1.p25-32

Abstract

Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.
Potensi Bakteri Filoplan Asal Tanaman Sagu Aksesi Makanaru (Metroxylon longispinum) Sebagai Agens Hayati Terhadap Pertumbuhan Rhizoctonia solani Kuhn Nurlette, Jahra; Leiwakabessy, Christoffol; Talahaturuson, Abraham; Uruilal, Costanza; Ririhena, Rhony Einstein; Rumahlewang, Wihelmina; Patty, Jacob Richard
JURNAL PERTANIAN KEPULAUAN Vol 8 No 1 (2024): Jurnal Pertanian Kepulauan
Publisher : Fakultas Pertanian, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jpk.2024.8.1.24

Abstract

The sago plant is a staple food for the people of Maluku and Papua and has many benefits and advantages to continue to be developed. A group of phylloplane microbes grow and spread on the surface of the leaves. The objective study is to find isolates of phylloplane bacteria from sago accession Makanaru and determine the inhibition of these bacteria against the growth of Rhizoctonia solani Kuhn. Sampling of sago plants (upper, middle, and lower leaves) was conducted in Negeri Seith, Leihitu District, Central Maluku Regency. Based on the isolation results, 21 isolates of phylloplanes bacteria were obtained and ready for further testing. From the results of hypersensitive test selection, 14 non-pathogenic isolates of phylloplane bacteria were obtained. Furthermore, tested the inhibitory power of phylloplane bacteria in suppressing the growth of the fungus, 3 phylloplane bacteria isolates were obtained with strong inhibitory, namely DA3.3, DT3.2, DT3.1 followed by 3 isolates DB4, DB4.2, and DB4.4 (medium inhibitory) which have the potential to be developed as biological agents.
Studi Kerusakan Umbi Kentang Akibat Cendawan Patogen Pascapanen pada Beberapa Pasar di Kota Ambon Samponu, Welmince; Leiwakabessy, Christoffol; Rumahlewang, Wihelmina; Uruilal, Costanza; Talahaturuson, Abraham
JURNAL PERTANIAN KEPULAUAN Vol 8 No 2 (2024): Jurnal Pertanian Kepulauan
Publisher : Fakultas Pertanian, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jpk.2024.8.2.49

Abstract

The study aims to determine the level of damage to potato tubers due to post-harvest pathogenic fungi attacks in several traditional markets in Ambon City. This study is a survey study at the Mardika, Passo, and Rumahtiga markets. The results of the study found one type of post-harvest pathogenic fungus that attacks potato tubers in traditional markets in Ambon City, namely Fusarium spp. which is the cause of dry rot disease in potato tubers. The level of damage to potato tubers due to Fusarium spp. attacks is classified as severe, found in the Mardika market at 70.81% and the Transit Passo market at 60.91%. While in the Rumahtiga market only 31.62% are classified as moderate damage.