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All Journal Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Journal of Biomedicine and Translational Research Indonesian Journal of Biotechnology MNJ (Malang Neurology Journal) Universa Medicina Jurnal Info Kesehatan
Tri Yudani MR
Laboratorium Biokimia - Biologi Molekuler Fakultas Kedokteran Universitas Brawijaya Malang
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Journal : MNJ (Malang Neurology Journal)

 1 
DOES THE COMBINATION OF ROSMARINUS OFFICINALIS AND CENTELLA ASIATICA NANOEMULSIONS AFFECT BDNF EXPRESSION IN THE GESTATIONAL DIABETES MELLITUS MODEL IN ZEBRAFISH LARVAE Khotimah, Husnul; Lamadi, Sutini; Amelia, Rizki; Arista, Devi Maya; Prastiwi, Fajar Dwi; Raras, Tri Yudani Mardining
MNJ (Malang Neurology Journal) Vol. 10 No. 2 (2024): July
Publisher : PERDOSSI (Perhimpunan Dokter Spesialis Saraf Indonesia Cabang Malang) - Indonesian Neurological Association Branch of Malang cooperated with Neurology Residency Program, Faculty of Medicine Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2024.010.02.08

Abstract

Background: Pancreatic β-cells compensate for the increased insulin demand due to increasing glucose levels during pregnancy or in gestational diabetes mellitus (GDM). The enzyme that regulates the rate of gluconeogenesis production is PEPCK. Circulatory and metabolic disturbances in the brain caused by hyperglycemia could affect brain-derived neurotrophic factor (BDNF) expression as an agent for neurodevelopment. Objective:  The purpose of this work is to ascertain the impact of combining nanoemulsions of Rosmarinus officinalis and Centella asiatica on the expression of BDNF and PEPCK. Methods: This study was a true experimental laboratory using zebrafish larvae. The larvae were exposed to 3% glucose and a combination of nanoemulsion extracts. The study sample of zebrafish larvae at 72 hpf (hour post fertilization) was divided into control, glucose, treatment 1, 2, and 3, with the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions at 2.5 μg/ml, 5 μg/ml, and 10 μg/ml, respectively. PEPCK and BDNF expression were measured by the real-time PCR method. Results: The expression of PEPCK increased significantly in the glucose group (3.05 ± 0.27). The combination of Rosmarinus officinalis and Centella asiatica nanoemulsions significantly decreased PEPCK (p-value 0,001 < 0.05). The BDNF level did not significantly differ (p-value 0.253 >0.05) among groups, but the highest level (1.06 ± 0.60) was gained in the T2 group, which received an embryonic medium (EM), 3% glucose, and the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions at 5μg/ml. Conclusion: The combination of Rosmarinus officinalis and Centella asiatica nanoemulsions significantly reduces PEPCK expression but does not affect BDNF expression.
Co-Authors AA Sudharmawan, AA AGUSTIN KRISNA WARDANI Agustin, Dita Paramytha Ainiyah, Durotun Ajrullah, Mauludy Jutta Anandasari , Noni Fidya Ayu Anandasari, Noni Fidya Ayu Angelica, Aina Ardyah, Naura Paramitha Cindy Arista, Devi Maya Gunawan, Ellen Fenix Heni Pujiastuti Husnul Khotimah IS HELIANTI Kartika Senjarini Kaspirayanthi, Ni Ketut Devy Kenty Wantri Anita, Kenty Wantri Kridayanti, Novalia Labes, Antje Lamadi, Sutini Maimun Zulhaidah Arthamin, Maimun Zulhaidah Pramesti, Nadya Shafa Prastiwi, Fajar Dwi Prawiro, Sumarno Reto Rahmadani, Nabila - Rike Oktarianti Rizki Amelia Subandi Subandi Susiati, Hani Syubbanul Wathon, Syubbanul Tatit Nurseta Triwahju Astuti Utami, Diah Ayu Wildan Seputra, Maulana Yudhanto, Hendy Setyo