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Journal : Jurnal Agroteknos

INDUKSI TUNAS GADUNG (Diocorea hispida Dennst) SECARA IN VITRO ARIF, NORMA; ANSI, AZHAR; WIJAYANTO, TEGUH
Jurnal Agroteknos Vol 4, No 3 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Yam (Dioscorea hispida Dennst) is a tuber crop that belonges to food sources and has not been widely recognized by the public. Aside from being a food ingredient, it can also be used as a natural pesticide. Until now, efforts to use the potential of yam have not been handled well. This research was conducted at the In vitro Laboratory of the Faculty of agriculture, Haluoleo University, from May to July 2014. The purpose of the study was to obtain the proper concentration of BAP to induce yam plant shoots in vitro. The research design used was a completely randomized design with 5 replications. One segment of the stem explants were grown on MS basic medium with different concentrations of BAP (0.5-4 ppm), with 5 replications and each replication consisted of 1 bottle containing 2 explants. Data were analyzed using Duncan's Multiple Range Test (DMRT). The research results showed that the addition of various concentrations of BAP affected the span of the emergence of the first shoots but did not affect the percentage of explants alive and growing shoots. BAP concentration of 2 mg / L was the optimal concentration to induce shoots of yam (Dioscorea hispida) with a span began to shoot appearance at week 4 after planting.
INDUKSI TUNAS PISANG KEPOK (Musa paradisiaca L.) MENGGUNAKAN KOMBINASI KINETIN DAN IAA SECARA IN VITRO BOER, DIRVAMENA; PRAWANSA, AGUNG; RAKIAN, TRESJIA CORINA; ARSYAD, MIRZA ARSIATY; ARIF, NORMA; MADIKI, ABDUL; ARSANA, I MADE WISANA
Jurnal Agroteknos Vol 14, No 2 (2024)
Publisher : Jurnal Agroteknos

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Abstract

This study aims to investigate the effect of combining Kinetin and IAA on the growth of kepok banana shoots in vitro. The research was conducted at the In Vitro Laboratory, Agrotechnology Unit, Faculty of Agriculture, Halu Oleo University, from January to April 2023. The study utilized a Completely Randomized Design (CRD) with six treatments: P1 = (Kinetin 1 mg L⁻¹ + IAA 2 mg L⁻¹), P2 = (Kinetin 3 mg L⁻¹ + IAA 4 mg L⁻¹), P3 = (Kinetin 5 mg L⁻¹ + IAA 6 mg L⁻¹), P4 = (Kinetin 2 mg L⁻¹ + IAA 1 mg L⁻¹), P5 = (Kinetin 4 mg L⁻¹ + IAA 3 mg L⁻¹), and P6 = (Kinetin 6 mg L⁻¹ + IAA 5 mg L⁻¹). Each treatment had five replicates, resulting in 30 experimental units, with four Kepok banana shoot explants per culture bottle. The observed variables included shoot emergence time, number of shoots, shoot length, and shoot growth percentage. Results indicated that the combination of Kinetin and IAA concentrations significantly affected the number of shoots and shoot emergence time and shoot length, but did not significantly affect the shoot growth percentage. The best treatment was P2 = (Kinetin 3 mg L⁻¹ + IAA 4 mg L⁻¹) for in vitro growth of Kepok banana shoots.