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<![CDATA[AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JERUK PURUT (Citrus hystrix) TERHADAP Staphylococcus aureus DAN Escherichia coli ]]>
<![CDATA[Yuliani, Ratna]]>
<![CDATA[ Pharmacon Vol 12, No 2 (2011) ]]>
Publisher : <![CDATA[Pharmacon ]]>
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<![CDATA[Jeruk purut (Citrus hystrix) merupakan tanaman dari suku Rutaceae yang telah lama dikenal masyarakat sebagai bahan cita rasa. Hasil penelitian sebelumnya melaporkan bahwa minyak atsiri jeruk nipis (Citrus aurantifolia) memiliki aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan untuk mengukur aktivitas antibakteri minyak atsiri daun jeruk purut terhadap Staphylococcus aureus dan Escherichia coli dan mendeteksi kandungan minyak atsiri yang mempunyai aktivitas antibakteri. Minyak atsiri daun jeruk purut yang diperoleh dengan cara destilasi uap dan air diuji aktivitas antibakterinya menggunakan metode dilusi cair. Kromatografi Lapis Tipis (KLT) dilakukan menggunakan silika gel GF254 dan heksan-etil asetat (9:1). Bercak hasil KLT dideteksi dengan UV254nm, UV366nm, anisaldehid-asam sulfat, dan vanilin-asam sulfat. Bioautografi dilakukan dengan metode kontak. Hasil uji aktivitas antibakteri menunjukkan bahwa minyak atsiri daun jeruk purut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dengan nilai Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) berturut-turut sebesar 1 dan 2%. Minyak atsiri juga mampu menghambat dan membunuh Escherichia coli dengan nilai KHM dan KBM £ 0,0625%. Hasil KLT menunjukkan bahwa minyak atsiri daun jeruk purut mengandung beberapa senyawa golongan terpen dengan Rf yang berbeda. Berdasarkan hasil bioautografi, salah satu senyawa golongan terpen tersebut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli.            Kata kunci : antibakteri, Citrus hystrix, E. coli, minyak atsiri, S. aureus]]>
<![CDATA[Skrining Aktivitas Antibakteri Ekstrak Etanol 70% Sepuluh Daun Tanaman Terhadap Klebsiella Pneumoniae ]]>
<![CDATA[Qolbi, Nurul]]>;
<![CDATA[Yuliani, Ratna]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 15, No 1 (2018) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
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DOI: 10.23917/pharmacon.v15i1.6169
<![CDATA[Klebsiella pneumoniae causes several diseases such as chronic obstructive lung disease and community pneumonia. The antibiotics used for treating the infectious diseases cause several side effects. Therefore, alternative medicines which have minimal side effects are needed. This research aimed to investigate antibacterial activity of ten ethanolic extract of leaves against K. pneumoniae, identify compounds and active compounds in extract which has the highest antibacterial activity. Compounds in leaves were extracted using 70% ethanol with maceration method. The disk diffusion method was used for screening antibacterial activity against K. pneumoniae. The identification of group of compounds in extract with the largest inhibitory zone diameter was done using Thin Layer Chromatography (TLC) and bioautographic test was performed to determine the active compounds. The results of antibacterial activity screening tests showed that all extracts have antibacterial activity except bamboo leaf extract. Breadfruit extract (Artocarpus altilis) has the highest antibacterial activity with diameter of inhibition zone of 18,33 ± 5,57 mm. Breadfruit extract contains tannin, polyphenol, alkaloid, terpenoid, saponin, and the active antibacterial compounds were terpenoid and saponin.]]>
<![CDATA[Uji Aktivitas Antibakteri Ekstrak Etanol Bawang Dayak (Eleutherine palmifolia (L.) Merr) Terhadap Bakteri Staphylococcus aureus ]]>
<![CDATA[Warsiti, Warsiti]]>;
<![CDATA[Wardani, Sisca Dwikusuma]]>;
<![CDATA[Ramadhan, Ardea Achmad]]>;
<![CDATA[Yuliani, Ratna]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 15, No 2 (2018) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
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DOI: 10.23917/pharmacon.v15i2.6526
<![CDATA[Staphylococcus aureus is an infectious bacteria with pus because of tissue damage. Infectious diseases caused by S. aureus include wound infections, ulcers, pimples, and impetigo. Dayak onions have the benefit of being antibacterial. This research aims to study the antibacterial activity of ethanolic extract of Dayak onions against Staphylococcus aureus. The antibacterial study was carried out using the disk diffusion method. Identification of bacteria by Gram staining showed Gram-positive bacteria which can ferment mannitol with the mannitol test. A bacterial sensitivity test was conducted using three antibiotics i.e. cefoxitin 30μg, vancomycin 30μg, and linezolid 30μg. The bacteria was sensitive to all of the three antibiotics, with the inhibitory zone of 23.5 mm; 17 mm and 27.5 mm, respectively. Vancomycin 30μg was used as a positive control, resulted in average diameter of the inhibitory zone of 17.83 2.25 mm, whereas a control solvent DMSO (Dimethyl sulphoxide) did not produce zones. The ethanolic extract of Dayak onion in the concentration of 25%; 50%; 75% and 100% showed the average diameter of inhibitory zones of 8.17 0.29 mm; 10.67 1.44 mm; 10.33 1.53 mm; and 12.33 1.61 mm, respectively]]>
<![CDATA[AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JERUK PURUT (Citrus hystrix) TERHADAP Staphylococcus aureus DAN Escherichia coli ]]>
<![CDATA[Yuliani, Ratna]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 12, No 2 (2011) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
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DOI: 10.23917/pharmacon.v12i2.31
<![CDATA[Jeruk purut (Citrus hystrix) merupakan tanaman dari suku Rutaceae yang telah lama dikenal masyarakat sebagai bahan cita rasa. Hasil penelitian sebelumnya melaporkan bahwa minyak atsiri jeruk nipis (Citrus aurantifolia) memiliki aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan untuk mengukur aktivitas antibakteri minyak atsiri daun jeruk purut terhadap Staphylococcus aureus dan Escherichia coli dan mendeteksi kandungan minyak atsiri yang mempunyai aktivitas antibakteri. Minyak atsiri daun jeruk purut yang diperoleh dengan cara destilasi uap dan air diuji aktivitas antibakterinya menggunakan metode dilusi cair. Kromatografi Lapis Tipis (KLT) dilakukan menggunakan silika gel GF254 dan heksan-etil asetat (9:1). Bercak hasil KLT dideteksi dengan UV254nm, UV366nm, anisaldehid-asam sulfat, dan vanilin-asam sulfat. Bioautografi dilakukan dengan metode kontak. Hasil uji aktivitas antibakteri menunjukkan bahwa minyak atsiri daun jeruk purut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dengan nilai Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) berturut-turut sebesar 1 dan 2%. Minyak atsiri juga mampu menghambat dan membunuh Escherichia coli dengan nilai KHM dan KBM £ 0,0625%. Hasil KLT menunjukkan bahwa minyak atsiri daun jeruk purut mengandung beberapa senyawa golongan terpen dengan Rf yang berbeda. Berdasarkan hasil bioautografi, salah satu senyawa golongan terpen tersebut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Kata kunci : antibakteri, Citrus hystrix, E. coli, minyak atsiri, S. aureus]]>
<![CDATA[HASIL SKRINING AKTIVITAS SITOTOKSIK EKSTRAK ETANOL DAUN KELENGKENG (DIMOCARPUS LONGAN), DAUN KERSEN (MUNTINGIA CALABURA), DAN DAUN ALPUKAT (PERSEA AMERICANA) TERHADAP SEL T47D DAN WIDR ]]>
<![CDATA[Yuliani, Ratna]]>;
<![CDATA[Santoso, Broto]]>;
<![CDATA[Permatasani, Bella]]>;
<![CDATA[Sari, Diah Mukti]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 2 (2019) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
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DOI: 10.23917/pharmacon.v16i2.9050
<![CDATA[Cancer treatments usually cause adverse drug reactions. Therefore, safe anticancer drugs are needed in the treatment of cancer. One source of medicine that can be explored is plant. Extracts of longan leaves (Dimocarpus longan), jamaican cherry leaves (Muntingia calabura), and avocado leaves (Persea americana) have been tested for cytotoxic activity against several cancer cell lines. This study aims to determine the cytotoxic activity of ethanolic extract of longan leaves, jamaican cherry leaves, and avocado leaves against T47D and WiDr cells and to identify secondary metabolites in the extracts which have the highest activity. Ethanolic extract of longan leaves, jamaican cherry leaves, and avocado leaves were tested for their cytotoxic activity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Identification of secondary metabolites in the ethanolic extract of avocado leaves was carried out by thin layer chromatography method using silica gel GF254 as the stationary phase and a mixture of n-hexane and acetone (6:4) as the mobile phase. Cytotoxic test results show that ethanolic extract of longan leaves and cherry leaves with concentration of up to 1600 ?g/mL do not reduce the T47D and WiDr living cells to 50%. Avocado leaf extract decreases the percentage of living T47D cells and WiDr with IC50 values of 790.679 µg/mL and 1072.2 µg/mL, respectively. The ethanolic extract of avocado leaves contains flavonoid, phenolic, and terpenoid. Ethanolic extract of longan leaves, cherry leaves and avocado leaves do not have cytotoxic activity against T47D and WiDr cells.]]>
<![CDATA[ETHANOLIC EXTRACT OF PAPAYA LEAVES (CARICA PAPAYA) AND ITS FRACTIONS HAVE NO POTENTIAL CYTOTOXICITY ON T47D CELLS ]]>
<![CDATA[Yuliani, Ratna]]>;
<![CDATA[Syahdeni, Faisal]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 17, No 1 (2020) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
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DOI: 10.23917/pharmacon.v17i1.10760
<![CDATA[Breast cancer is one type of cancer that causes high mortality rate in women. Plants produce secondary metabolites with a wide range of activity, one of which is as an anticancer. This research was conducted to investigate the cytotoxic effect of ethanolic extract of papaya leaves (Carica papaya) and its fractions on T47D cell line. Extraction of the natural compounds from papaya leaves was carried out by soaking the leaf powder in 96% ethanol followed by filtration and solvent evaporation. Liquid partition was done to obtain hexane, ethyl acetate, and water fractions. The cytotoxicity of the extract and the fractions was tested using MTT assay. Identification of chemical compounds in the ethyl acetate fraction was conducted using a thin layer chromatography method that used silica gel GF 254 as the stationary phase and acetone and hexane (4:6) as the mobile phase. The MTT assay result shows that the extract does not inhibit the growth of T47D cells. The viability of T47D cells can be reduced by the hexane, ethyl acetate, and water fractions with IC50 values of 2,231.30, 557.33, and 2,112.81 ?g/mL, respectively. The ethyl acetate fraction contains flavonoids, alkaloids, phenolics, and terpenoids. The ethanolic extract of papaya leaves and the fractions has no potential cytotoxicity on T47D cells due to high IC50 values.]]>
<![CDATA[Skrining Aktivitas Antibakteri Ekstrak Etanol 70% Sepuluh Daun Tanaman Terhadap Klebsiella Pneumoniae ]]>
<![CDATA[Nurul Qolbi]]>;
<![CDATA[Ratna Yuliani]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 15, No 1 (2018) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
Show Abstract
|
Download Original
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Check in Google Scholar
|
DOI: 10.23917/pharmacon.v15i1.6169
<![CDATA[Klebsiella pneumoniae causes several diseases such as chronic obstructive lung disease and community pneumonia. The antibiotics used for treating the infectious diseases cause several side effects. Therefore, alternative medicines which have minimal side effects are needed. This research aimed to investigate antibacterial activity of ten ethanolic extract of leaves against K. pneumoniae, identify compounds and active compounds in extract which has the highest antibacterial activity. Compounds in leaves were extracted using 70% ethanol with maceration method. The disk diffusion method was used for screening antibacterial activity against K. pneumoniae. The identification of group of compounds in extract with the largest inhibitory zone diameter was done using Thin Layer Chromatography (TLC) and bioautographic test was performed to determine the active compounds. The results of antibacterial activity screening tests showed that all extracts have antibacterial activity except bamboo leaf extract. Breadfruit extract (Artocarpus altilis) has the highest antibacterial activity with diameter of inhibition zone of 18,33 ± 5,57 mm. Breadfruit extract contains tannin, polyphenol, alkaloid, terpenoid, saponin, and the active antibacterial compounds were terpenoid and saponin.]]>
<![CDATA[AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN JERUK PURUT (Citrus hystrix) TERHADAP Staphylococcus aureus DAN Escherichia coli ]]>
<![CDATA[Ratna Yuliani]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 12, No 2 (2011) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
Show Abstract
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DOI: 10.23917/pharmacon.v12i2.31
<![CDATA[Jeruk purut (Citrus hystrix) merupakan tanaman dari suku Rutaceae yang telah lama dikenal masyarakat sebagai bahan cita rasa. Hasil penelitian sebelumnya melaporkan bahwa minyak atsiri jeruk nipis (Citrus aurantifolia) memiliki aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan untuk mengukur aktivitas antibakteri minyak atsiri daun jeruk purut terhadap Staphylococcus aureus dan Escherichia coli dan mendeteksi kandungan minyak atsiri yang mempunyai aktivitas antibakteri. Minyak atsiri daun jeruk purut yang diperoleh dengan cara destilasi uap dan air diuji aktivitas antibakterinya menggunakan metode dilusi cair. Kromatografi Lapis Tipis (KLT) dilakukan menggunakan silika gel GF254 dan heksan-etil asetat (9:1). Bercak hasil KLT dideteksi dengan UV254nm, UV366nm, anisaldehid-asam sulfat, dan vanilin-asam sulfat. Bioautografi dilakukan dengan metode kontak. Hasil uji aktivitas antibakteri menunjukkan bahwa minyak atsiri daun jeruk purut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dengan nilai Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) berturut-turut sebesar 1 dan 2%. Minyak atsiri juga mampu menghambat dan membunuh Escherichia coli dengan nilai KHM dan KBM £ 0,0625%. Hasil KLT menunjukkan bahwa minyak atsiri daun jeruk purut mengandung beberapa senyawa golongan terpen dengan Rf yang berbeda. Berdasarkan hasil bioautografi, salah satu senyawa golongan terpen tersebut mempunyai aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Kata kunci : antibakteri, Citrus hystrix, E. coli, minyak atsiri, S. aureus]]>
<![CDATA[Uji Aktivitas Antibakteri Ekstrak Etanol Bawang Dayak (Eleutherine palmifolia (L.) Merr) Terhadap Bakteri Staphylococcus aureus ]]>
<![CDATA[Warsiti Warsiti]]>;
<![CDATA[Sisca Dwikusuma Wardani]]>;
<![CDATA[Ardea Achmad Ramadhan]]>;
<![CDATA[Ratna Yuliani]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 15, No 2 (2018) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
Show Abstract
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Download Original
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DOI: 10.23917/pharmacon.v15i2.6526
<![CDATA[Staphylococcus aureus is an infectious bacteria with pus because of tissue damage. Infectious diseases caused by S. aureus include wound infections, ulcers, pimples, and impetigo. Dayak onions have the benefit of being antibacterial. This research aims to study the antibacterial activity of ethanolic extract of Dayak onions against Staphylococcus aureus. The antibacterial study was carried out using the disk diffusion method. Identification of bacteria by Gram staining showed Gram-positive bacteria which can ferment mannitol with the mannitol test. A bacterial sensitivity test was conducted using three antibiotics i.e. cefoxitin 30μg, vancomycin 30μg, and linezolid 30μg. The bacteria was sensitive to all of the three antibiotics, with the inhibitory zone of 23.5 mm; 17 mm and 27.5 mm, respectively. Vancomycin 30μg was used as a positive control, resulted in average diameter of the inhibitory zone of 17.83 2.25 mm, whereas a control solvent DMSO (Dimethyl sulphoxide) did not produce zones. The ethanolic extract of Dayak onion in the concentration of 25%; 50%; 75% and 100% showed the average diameter of inhibitory zones of 8.17 0.29 mm; 10.67 1.44 mm; 10.33 1.53 mm; and 12.33 1.61 mm, respectively]]>
<![CDATA[Ethanolic Extract of Papaya Leaves (Carica papaya) and its Fractions have no Potential Cytotoxicity on T47D Cells ]]>
<![CDATA[Ratna Yuliani]]>;
<![CDATA[Faisal Syahdeni]]>
<![CDATA[ Pharmacon: Jurnal Farmasi Indonesia Vol 17, No 1 (2020) ]]>
Publisher : <![CDATA[Universitas Muhammadiyah Surakarta ]]>
Show Abstract
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Download Original
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Original Source
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DOI: 10.23917/pharmacon.v17i1.10760
<![CDATA[Breast cancer is one type of cancer that causes high mortality rate in women. Plants produce secondary metabolites with a wide range of activity, one of which is as an anticancer. This research was conducted to investigate the cytotoxic effect of ethanolic extract of papaya leaves (Carica papaya) and its fractions on T47D cell line. Extraction of the natural compounds from papaya leaves was carried out by soaking the leaf powder in 96% ethanol followed by filtration and solvent evaporation. Liquid partition was done to obtain hexane, ethyl acetate, and water fractions. The cytotoxicity of the extract and the fractions was tested using MTT assay. Identification of chemical compounds in the ethyl acetate fraction was conducted using a thin layer chromatography method that used silica gel GF 254 as the stationary phase and acetone and hexane (4:6) as the mobile phase. The MTT assay result shows that the extract does not inhibit the growth of T47D cells. The viability of T47D cells can be reduced by the hexane, ethyl acetate, and water fractions with IC50 values of 2,231.30, 557.33, and 2,112.81 g/mL, respectively. The ethyl acetate fraction contains flavonoids, alkaloids, phenolics, and terpenoids. The ethanolic extract of papaya leaves and the fractions has no potential cytotoxicity on T47D cells due to high IC50 values.]]>
