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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN BIOTROPIA - The Southeast Asian Journal of Tropical Biology Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Window of Health : Jurnal Kesehatan Jurnal Kesehatan Prima BERNAS: Jurnal Pengabdian Kepada Masyarakat Pelita: Jurnal Pengabdian kepada Masyarakat Prosiding Seminar Kesehatan Masyarakat Prosiding Seminar Nasional Unimus
Ragil Saptaningtyas, Ragil
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PRODUCTION OPTIMIZATION, PARTIAL PURIFICATION, AND THROMBOLYTIC ACTIVITY EVALUATION OF PROTEASE OF Bacillus cereus HSFI-10 Ainutajriani, Ainutajriani; Darmawati, Sri; Zilda, Dewi Seswita; Afriansyah, Muhammad Ardi; Saptaningtyas, Ragil; Ethica, Stalis Norma
BIOTROPIA Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1765

Abstract

Cardiovascular disease is the primary cause of mortality in the world due to the formation of blood clots or thrombi in blood vessels. Bacterial proteases commonly function as thrombus dissolver agents in the pharmaceutical industry. Bacterial isolate HSFI-10 (Holothuria scabra Fermented Intestine-10) previously isolated from Rusip fermented sea cucumber had demonstrated thrombolytic activity. This study aimed to produce crude protease of HSFI-10 strain at an optimized incubation time and determine the thrombolytic activity of crude and dialysate proteases on A, B, AB, and O blood types. Isolate HSFI-10 was first molecularly identified and found to be Bacillus cereus with a homology level of 99.80% with Bacillus cereus strain ST06. The optimum crude enzyme was obtained after 48-h incubation with an activity of 222.52 U/mL, which increased to 438.84 U/mL after ammonium sulfate precipitation and dialysis. Clot lysis activity of crude enzymes was measured based on the gravimetry method on blood in the ABO system, showing results that ranged from 68.99% to 69.76%, while the dialysate ranged from 81.16% to 82.52%. In conclusion, partial purification of bacterial protease could increase both its specific and thrombolytic activities on human blood in the ABO system, with only 1% activity variability between A, B, AB, and O blood types.
Co-Authors Afriansyah, Muhammad Ardi Ainutajriani, Ainutajriani Ana Hidayati Mukaromah Andri Sukeksi Annisa Nurul Hikmah Aprilia Indra Kartika, Aprilia Indra Chairunnisaa, Aretha Dewi Seswita Zilda ERMA WIDYA LESTARI, ERMA Fandhi Adi Wardoyo Fauziati, Aditia Maulida Gela Setya Ayu Putri Hikmah, Murti Amalia Nur Izza, Naimatul Joko Teguh Isworo Kurniawan, Sodikin Lestari, Yunita Liza, Novita Vera Maharani, Alifia Inggit Mohd Hanif Zulfakar Musiati, Wa Ode Nadia Maharani Ningrum, Nila Cahya Nova, Dini Adelia Orlin, Diana Prititasari, Rahma Putri Sulfitri Rahajoe, Toeti Renaldi, Muhamad Sari, Sri Mayang Sefrianti, Putri Amelia Shofa Chasani Shofwana, Fatya Sri Darmawati Sri Setianingsih, Sri Stalis Norma Ethica Taufiqurrachman Nasihun Wulandari, Nungky