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Efraim Samson, Efraim
Department Of Biology, Universitas Kristen Satya Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Journal : biofaal journal

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ANALISIS FILOGENETIK GEN gyrA DAN gyrB DARI GENUS MESORHIZOBIUM, RHIZOBIUM DAN ENSIFER Apituley, Edwin Thomas; Samson, Efraim; Killay, Amos
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp118-127

Abstract

Nucleotide sequence of gyrA and gyrB genes encoding subunit A and B of gyrase enzyme from genus of Rhizobium, Ensifer and Mesorhizobium were analyzed to determine evolutionary relationship among them. Maximum Likelihood and Kimura 2 parameter methods were used to construct phylogenetic tree and measure genetic distance. Phylogenetic tree based on nucleotide sequence of gyrA gene was compared to phylogenetic tree based on nucleotide sequence of gyrB gene. Comparison of identity percentage among nucleotide sequence of gyrA gene from genus Rhizobium, Ensifer and Mesorhizobium show similarity, where each of them have narrow range of identity percentage, however nucleotide sequence of gyrB gene from genus Rhizobium and Ensifer show wider identity percentage range than genus Mesorhizobium. Several member of genus Rhizobium and Ensifer have double copy of gyrB gene with identity percentage less than 50 percent between them. Topology of phylogenetic tree based on nucleotide sequence of gyrA gene have similar topology to topology of phylogenetic tree based on nucleotide sequence of gyrB gene, except for additional branch formed by one of additional copy of gyrB sequences. .
POTENSI KULIT BATANG Cinnamomum burmannii Bl. DALAM MENCEGAH INFERTILITAS; KAJIAN TERHADAP BERAT TESTIS DAN JUMLAH SPERMATOZOA MENCIT (Mus musculus) MODEL DIABETES MELLITUS TIPE-1 Moniharapon, Mechiavel; Ukratalo, Abdul M; Pattimura, Nadira; Samson, Efraim; Pangemanan, Victory O
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp108-117

Abstract

In individuals with diabetes mellitus, the increase in Reactive Oxygen Species (ROS) can damage the mitochondrial membrane, leading to the loss of potential mitochondrial membrane function in inducing apoptosis of sperm cells. Cinnamomum burmannii is a type of spice that contains antioxidants, thus capable of neutralizing free radicals and reducing oxidative stress caused by diabetes mellitus. Flavonoids also have an effect on increasing spermatogenesis. The aim of this research is to examine the role of methanol extract from Cinnamomum burmannii bark in increasing testis weight and spermatozoa count in type-1 diabetes mellitus mice (Mus musculus). The study employs a Completely Randomized Design (CRD) with five treatments and three replications. Twenty mice are divided into groups: K- (control), K+ mice injected with STZ without medication), P1 (dose of 200 mg/kg BW), P2 (dose of 400 mg/kg BW), and P3 (dose of 800 mg/kg BW). Before administering the Cinnamomum burmannii bark extract, blood sugar levels are measured in the K+, P1, P2, and P3 groups. If blood sugar levels have increased (>128 mg/dL), the P1, P2, and P3 groups are given the Cinnamomum burmannii bark extract according to the predetermined dosage. The treatment is carried out for 14 days. On the last day, surgery is performed to extract the testes and observe the spermatozoa count. The observation data on testis weight and spermatozoa count will be analyzed using ANOVA with SPSS 24.0. The results indicate that the administration of methanol extract from Cinnamomum burmannii bark significantly affects the increase in testis weight and spermatozoa count in type-1 diabetes mellitus mice. The increase in testis weight and spermatozoa count is proportional to the dosage given
PERUBAHAN SIFAT-SIFAT KIMIA PADA TANAH ULTISOL PASCA PEMBERIAN PENGAPURAN DAN KOTORAN KELELAWAR, SERTA EFEKNYA TERHADAP HASIL BIJI KERING KACANG TANAH (Arachis hypogeae L.) Silahooy, Charles; Unitly, Adrien Jems Akiles; Silahooy, Veince Benjamin; Samson, Efraim; Apituley, Edwin T
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp001-009

Abstract

Penelitian ini bertujuan untuk mengetahui perubahan sifat-sifat kimia pada tanah ultisol pasca pemberian pengapuran dan kotoran kelelawar, serta efeknya terhadap hasil biji kering kacang tanah (Arachis hypogeae L.). Penelitian ini dilaksanakan pada rumah plastik di Desa Ouw, Kabupaten Maluku Tengah, berlangsung dari bulan Oktober 2023 sampai Maret 2024. Analisis sifat-sifat tanah dilakukan di Laboratorium Tanah, Fakultas Pertanian Universitas Pattimura. Rancangan percobaan yang digunakan dalam penelitian ini adalah Rancangan Acak Langkap (RAL) berpola faktorial 4 x 4 x 4, dimana parameter yang diteliti adalah pH tanah (H20 1 : 2,5), kemasaman dapat tukar (Aldd + Hdd), Kadar Cadd, Kadar Mgdd dan berat biji kering per tanaman. Hasil penelitian menunjukkan bahwa peranan kapur terutama dalam menaikkan pH tanah dan menurunkan kemasaman dapat tukar (Aldd + Hdd), sedangkan peranan kotoran kelelawar terutama dalam menaikkan kadar Cadd, Mgdd dan hasil biji kering kacang tanah pada tanah ultisol. Selain itu, Hasil setara biji kering kacang tanah terbaik (18,58g / tanaman) diproleh pada taraf kotoran kelelawar 192g per pot tanpa pengapuran pada tanah ultisol dengan pH tanah 5,3 dan kejenuhan Al 66,22%.
Co-Authors Adrien Jems Akiles Unitly Amos Killay Apituley, Edwin T Apituley, Edwin Thomas CECILIA ANNA SEUMAHU Chomsa Dintasari Umi Baszary Daniati Kasale Deli Wakano Deli Wakano Deli Wakano Ferdy S. Rondonuwu Ferdy S. Rondonuwu Haryono Semangun Haryono Semangun Hitalesiakwane, Yesty E. L D Tetelepta La Eddy Martha Kaihena Melati Sopacua Moniharapon, Mechiavel Nadira Pattimura Pangemanan, Victory O Silahooy, Charles Silahooy, Veince Benjamin Ukratalo, Abdul M Umi Kalsum Umi Kalsum Vergenia Sigmarlatu Wahyudi Abdul Hamid Ridwan Wakano, Deli Wempi Elwuar