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All Journal Disease Prevention and Public Health Journal Universa Medicina Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Nurmasitoh, Titis
Departemen Fisiologi Fakultas Kedokteran Universitas Islam Indonesia
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Journal : Universa Medicina

 1 
Honey improves lipid profile of diet-induced hypercholesterolemic rats Nurmasitoh, Titis; Pramaningtyas, Miranti Dewi
Universa Medicina Vol 34, No 3 (2015)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.177-186

Abstract

BackgroundCoronary heart disease (CHD) is a major cause of morbidity and mortality throughout the world, including Indonesia. One of the risk factors for CHD is hypercholesterolemia. One of the natural products that has been developed for the treatment of hypercholesterolemia is honey. Honey contains fructooligosaccharides, various vitamins, minerals, and enzymes which are supposedly able to lower blood cholesterol levels. This research aimed to study the influence of honey on the levels of blood total cholesterol, triglyceride, and low density lipoprotein (LDL) levels in Wistar rats. MethodsThis study was of experimental post test control group design. Twenty-four male Wistar rats (Rattus norvegicus) were randomly divided into 4 groups. K1 was the negative control group (with normal diet), K2 was the positive control group (with high-fat diet), P1 was fed a high-fat diet for 7 days, followed by high-fat diet plus honey for the next 7 days. P2 was fed a high-fat diet for 7 days, followed by regular diet plus honey for the next 7 days. After completion of this treatment, total cholesterol, triglycerides, and LDL levels were measured by the cholesterol oxidase phenol+aminophenazone (CHOD-PAP) method using enzymatic spectrophotometry principles. ResultsThere were significant differences in total cholesterol, triglyceride, and LDL levels between all groups after day 15 (p<0.05). ConclusionHoney supplementation was able to reduce the blood levels of total cholesterol, triglycerides, and LDL. Honey supplementation accompanied by non-cholesterol feeds could more effectively lower total cholesterol, triglycerides, and LDL serum levels in Wistar rats.
Black sugarcane decoction reduces rat brain ischemia Handayani, Ety Sari; Nugraha, Zainuri Sabta; Nurmasitoh, Titis; Kuswati, Kuswati; Ahsani, Dwi N.; Nanda, Ajeng G.
Universa Medicina Vol 35, No 1 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.40-45

Abstract

BackgroundThere are people in Yogyakarta, who use black sugarcane decoction (BSD) to prevent stroke. BSD contains policosanol and antioxidants. It has been proven that policosanol can reduce global ischemia in Mongolian gerbils. This study aims to evaluate the effect of BSD on brain ischemia in a rat stroke model. MethodsA laboratory experiment using eighteen 3-month old male Wistar rats without any defects, of 175-250 g body weight. Brain ischemia was produced by a 20-minute bilateral carotid communis artery oclusion (BCCAO).  Using a rat stroke model, brain ischemia was produced by a 20-minute BCCAO. The rats were randomized into three groups: BSD treated stroke model rats (group 1), non treated stroke model rats (group 2), and sham operated rats (group 3). BSD was administered by gavage for 1 week before BCCAO. Decapitation of rats was performed two hours post BCCAO. Brain tissues were stained with 2,3,5-triphenyltetrazolium chloride (TTC). Ischemic areas were analyzed using Image J softwere. Statistical analysis was conducted by one way ANOVA test.ResultsThe mean percentages of rat brain ischemic area differed between group 3 (0.0 ± 0.0%), group 2 (3.13 ± 0.59%) and group 1 (1.15 ± 0.47%) p =0.001). Post hoc test showed that there was no difference between group 3 with group 1. Instead, there was a significant difference between  group 2 and the other groups.ConclusionThe administration of BSD reduced rat brain ischemia after bilateral carotid artery ligation.
Shortening tends to increase aortic foam cell count and wall thickness in male Wistar rats Lusiantari, Rokhima; Pramaningtyas, Miranti Dewi; Nurmasitoh, Titis; Pattimura, Rachmi Hidayati; Dewanti, Anggita
Universa Medicina Vol 37, No 1 (2018)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2018.v37.13-18

Abstract

BackgroundShortening is widely used as raw material for bread and other foods. Hypercholesterolemia increases aortic arch foam cell formation and abdominal aortic wall thickness. This study aimed to determine the effect of shortening on the number of aortic arch foam cells and abdominal aortic wall thickness in rats.MethodsThis study was of experimental posttest control group design. Twenty four male Wistar rats were randomized into 4 groups. The negative control group (C-) received standard feed, the positive control group (C+) standard high-fat feed, group T1 shortening and standard feed at a ratio of 1:5 and group T2 shortening and standard feed at a ratio of 1:10. The interventions were given for 6 weeks through gavage. The foam cell count in the aortic arch and the thickness of the abdominal aortic wall were measured. One-way ANOVA test was used to analyze the data. ResultsThere was no significant difference in the mean foam cell count of the aortic arch between the four groups C- (7.17 ± 4.17), C + (9.33 ± 7.01), T1 (11.83 ± 4.88) and T2 (9.33 ± 6.80) (p=0.598). The mean thickness of the abdominal aortic wall between the four groups C- (741.98 ± 60.67μm), C + (714.29 ± 90.59μm), T1 (838.90 ± 75.86 μm), and T2 (749.88 ± 99.37μm) also was not significantly different (p=0.110).Conclusion Shortening tends to increase the foam cell count of the aortic arch and the thickness of the abdominal aortic wall of rats.
Intermittent fasting decreases oxidative stress parameters in Wistar rats (Rattus norvegicus) Nurmasitoh, Titis; Utami, Shindy Yudha; Kusumawardani, Endah; Najmuddin, Abdulhalim Ahmad; Fidianingsih, Ika
Universa Medicina Vol 37, No 1 (2018)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2018.v37.31-38

Abstract

BackgroundChronic and degenerative diseases are closely related to modern lifestyles that tend to be deficient in physical activity but excessive in food intake. One method used to overcome this problem is dietary restriction through intermittent fasting. Intermittent fasting decreases the risk of chronic and degenerative diseases, e.g. by lowering oxidative stress. Oxidative stress can be determined from the malondialdehyde (MDA) levels and lipid profile in the blood. The present study aimed to determine the effect of intermittent fasting on plasma MDA levels and lipid profile of Wistar rats (Rattus norvegicus).MethodsThis research was a laboratory experimental research using a post test control group design. Twenty four male Wistar rats (Rattus norvegicus) were randomly assigned to 4 groups, ie control group (C), fasting group (F), non-fasting obese group (NFO), and fasting obese group (FO). Fasting treatment given in this research was intermittent fasting, comprising one day of fasting for 12 hours alternating with one day of normal feed ad libitum in the span of 8 weeks. After completion of treatment, blood was taken transcardially for examination of MDA levels and lipid profiles using spectrophotometry.ResultsThere were significant between-group differences in MDA levels and lipid profiles (p<0.05 for all parameters). Groups treated with intermittent fasting had lower levels of MDA, total cholesterol, triglycerides, and ow-density lipoprotein (LDL) than those without intermittent fasting. The high-density lipoprotein (HDL) levels were higher in the intermittent fasting group than the other groups.ConclusionIntermittent fasting has an effect on the oxidative stress parameters of Wistar rats (Rattus norvegicus).
Mild anxiety and depression related to elevated dopamine level Fidianingsih, Ika; Nurmasitoh, Titis; Arjana, Adika Zhulhi; Devita, Ninda; Khoiriyah, Umatul
Universa Medicina Vol 38, No 1 (2019)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (788.451 KB) | DOI: 10.18051/UnivMed.2019.v38.48-55

Abstract

BackgroundDopamine is an important neurotransmitter that plays a role in the pathogenesis of anxiety and depression. Dopamine secretion occurs when there is a pleasurable stimulus. Blood dopamine levels have the potential to be developed as biomarkers of depression or anxiety, but previous studies related to the relationship between dopamine levels and levels of anxiety and depression are still controversial. The purpose of this study was to determine the correlation between anxiety and depression score with dopamine level in young adults. MethodsThis was an observational cross sectional study. A total of 43 subjects aged 18 to 40 years were recruited by consecutive non-random sampling according to inclusion and exclusion criteria. Anxiety and depression were assessed using the Beck anxiety inventory and Beck depression inventory-II questionnaire. Dopamine plasma level was measured using the enzyme-linked immunosorbent assay (ELISA). The Spearman rho correlation test was used to analyze the data and p<0.05 was considered significant.Results The median dopamine levels in respondents with mild, moderate and severe anxiety were respectively: 77.79; 63.43; 62.51 ng/mL (p=0.043). The median dopamine levels in respondents with reasonable depression, mood disorder, moderate and severe depression were respectively: 79.2; 61,32; 62,51; 60.24 (p=0.001). Correlation test results showed a weak correlation between dopamine and anxiety (r=- 0.310) and a moderate correlation for depression (r=-0.505).ConclusionMild anxiety and depression were associated with elevated dopamine level in young adults. Further studies are required to confirm the role of dopamine metabolism in anxiety and depression in young adults.
Co-Authors Adika Zhulhi Arjana Afid, Muhammad Dimas Afid, Muhammad Dimas Ajeng G. Nanda, Ajeng G. Asyahidatul Muchlisah Fitriana Bowo, Endrawati Tri Bunga Nirwani Cahyaningrum, Yeny Dyah Dewa Ayu Citra Dewanti, Anggita Dwi N. Ahsani, Dwi N. Endrawati Tri Bowo Ety Sari Handayani Farida Juliantina Rachmawaty Ika Fidianingsih Istiqomah Kurniawati Khoiriyah, Umatul Kusumawardani, Endah Kuswati Kuswati Lusiantari, Rokhima Mahardina, Dewa Ayu Citra Miranti Dewi Pramaningtyas Najmuddin, Abdulhalim Ahmad Ninda Devita, Ninda Nirwani, Bunga Pattimura, Rachmi Hidayati Rosmelia Malik Taufik Nur Yahya Utami, Shindy Yudha Wijaya, Diani Puspa Yanasta Yudo Pratama Zainuri Sabta Nugraha, Zainuri Sabta