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All Journal Bioscience Quagga Elkawnie Jurnal Kelautan Tropis Universa Medicina Jurnal Biologi Tropis Jurnal Biotek Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Kenanga : Journal of Biological sciences and Applied Biology Prosiding Seminar Nasional Universitas Serambi Mekkah Aksi Nyata : Jurnal Pengabdian Sosial dan Kemanusiaan Bumi: Jurnal Hasil Kegiatan Sosialisasi Pengabdian Kepada Masyarakat Jurnal Pengabdian Masyarakat Indonesia
Arif Sardi
Universitas Islam Negeri Ar-Raniry
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Journal : Elkawnie

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Biometric Condition of Seurukan Fish (Osteochillus Vittatus Valenciennes, 1842) Exposed to Mercury in Krueng Sabee River Aceh Jaya Indonesia Ilham Zulfahmi; Yunina Rahmi; Arif Sardi; Mahyana Mahyana; Yusrizal Akmal; Rumondang Rumondang; Epa Paujiah
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 1 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i1.8258

Abstract

Abstract: Mercury is a dangerous contaminant for aquatic organisms. Seurukan fish (Osteochilus vittatus) is a type of fish in the Krueng Sabee river that is vulnerable to mercury exposure. The study's purposes were to investigate the effect of mercury on the biometric conditions of Seurukan Fish in the Krueng Sabee River, Aceh Jaya Regency. A total of 90 Seurukan fish consisting of 50 males and 40 females were collected from 3 research stations. The research stations represent the upstream area (Station 1), median river bodies (Station 2), and the downstream area (Station 3). Fish samples were taken from July to August 2019. The main parameters observed included class interval, sex ratio, length-weight relationship, condition factors, mercury concentration in sediment and liver, and hepatosomatic index. The results showed that the mercury content in the sediments of the Krung Sabee River was increased both spatially and temporally. Station 1, located in the upstream area, has the highest mercury content in the sediment was 6.278 ± 0.987 mg/kg. Mercury content in liver of Seurukan Fish ranged from 0.182 ± 0.100 mg/kg to 0.198±0.152 mg/kg. Mercury contamination in the Krueng Sabee river caused a decrease in biometric conditions of Seurukan Fish. Seurukan Fish exposed to mercury tended to have smaller size, an unbalanced sex ratio, low hepatosomatic index value, and negative allometric growth pattern.Abstrak: Merkuri merupakan salah satu jenis kontaminan berbahaya bagi organisme akuatik. Ikan seurukan (Osteochilus vittatus) merupakan salah satu jenis ikan di sungai Krueng Sabee yang rentan terpapar merkuri. Penelitian ini bertujuan untuk mengkaji pengaruh merkuri terhadap kondisi biometrik ikan seurukan di sungai Krueng Sabee, Kabupaten Aceh Jaya. Sebanyak 90 ekor ikan Seurukan yang terdiri dari 50 ekor jantan dan 40 ekor betina dikoleksi dari 3 stasiun penelitian. Stasiun penelitian mewakili wilayah hulu (stasiun 1), badan sungai (stasiun 2) dan wilayah hilir (stasiun 3). Pengambilan  sampel ikan dilakukan pada bulan Juli hingga Agustus 2019. Parameter utama yang diamati meliputi selang kelas, nisbah kelamin dan hubungan panjang bobot ikan, faktor kondisi, konsentrasi merkuri pada sedimen dan hati serta indeks hepatosomatik. Hasil penelitian menunjukkan bahwa kandungan merkuri di sedimen Sungai Krung Sabee mengalami peningkatan baik secara spasial dan temporal. Stasiun 1 yang terletak di wilayah hulu memiliki kandungan merkuri dalam sedimen paling tinggi yaitu sebesar 6,278 ± 0,987 mg/kg. Kandungan merkuri pada hati ikan seurukan di Sungai Krueng Sabee berkisar antara 0,182 ± 0,100 mg/kg hingga 0,198 ± 0,152 mg/kg. Kontaminasi merkuri pada sungai Krueng Sabee menyebabkan dampak negatif terhadap kondisi biometrik ikan seurukan. Ikan seurukan yang terpapar merkuri cenderung memiliki ukuran panjang dan bobot yang lebih kecil, nisbah kelamin yang tidak seimbang, dan nilai indeks hepatosomatik yang rendah serta pola pertumbuhan alometrik negatif.
Konstruksi Primer Untuk Mendeteksi Mutasi Gen rpoB Mycobacterium tuberculosis Dengan Metode Amplification Refractory Mutation System (ARMS)-PCR Arif Sardi
Elkawnie: Journal of Islamic Science and Technology Vol 1, No 1 (2015)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v%vi%i.516

Abstract

Quickly recognize drug resistance in Mycobacterium tuberculosis is very important for the efficiency of the treatment and control of the disease. More than 95% of RIF resistance occurs due to point mutations in the 81-bp region of the rpoB gene M. tuberculosis. Generally, the mutation occurs at codon 531, 526 and 516. One of method to detect the mutation is using ARMS-PCR. In order to apply the ARMS-PCR technique to detection of mutations needed a special and specific primer. Primer design process using computer software "Primer Designer" and the specificity was confirmed with "Bioedit". Accuracy and ability of all these primers to detecting mutation in rpoB gene M. tuberculosis tested using ARMS-PCR method. PCR amplification results were then analyzed using the techniques of electrophoresis on agarose 1.5%. Electrophoresis results showed two bands produced from the amplification with the template H37RV (wild type). Both bands are expected to be in a position 238 bp and 484 bp, in the other hand the result amplification with the template from mutant strains produce one band with sized 484 bp. From these data it is known that ARMS-PCR reactions are performed can be used to detect mutations in the rpoB gene of M. Tuberculosis.
Co-Authors Asman Manaf Dwi Intan Hardila Dwi Yuliandhani Epa Paujiah, Epa Ernia Wati Feizia Huslina Ilham Zulfahmi Jamaluddinsyah Jamaluddinsyah Jamaluddinsyah Jamaluddinsyah Jamsari Jamsari Khairatul Ulya Lina Rahmawati Mahyana Mahyana Maria Maria lisa ML Edy Parwanto Muliari Muliari Nabilla Munawarah Nabilla Munawarah Nailul Muna Nailul Muna Nanda Vidasari Nanda Vidasari Neri Kautsari Novan Karnanto Raharjo Putri Magfirah R Nurlian R Nurlian Raudhah Hayatillah Rizky Ahadi Rosmeri Rosmeri Syafrina Sari Lubis Syamsul Arifin Syamsurizal Syamsurizal Tanwirul Anwar Yanwirasti Yanwirasti Yunina Rahmi Yusrizal Akmal