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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science Jurnal Pengendalian Hayati BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIKA Cropsaver : Journal of Plant Protection Plumula : Berkala Ilmiah Agroteknologi Proceeding International Conference on Global Resource Conservation Jurnal Agroekoteknologi BIOEDUSCIENCE Jurnal SOLMA Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah RESPATI Agrotechnology Research Journal Jurnal Pembelajaran dan Biologi Nukleus Agrienvi : Jurnal Ilmu Pertanian Nusantara Science and Technology Proceedings Berkala Penelitian Hayati AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Eduvest - Journal of Universal Studies Journal of Applied Plant Technology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Arika Purnawati
Agrotechnology, Faculty Of Agriculture, Universitas Pembangunan Nasional “Veteran” East Java, Surabaya 60294, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Social Sciences Veterinary Other

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Journal : Cropsaver : Journal of Plant Protection

 1 
Antagonistic Test of Bacillus spp. against Fusarium sp., the Causal Agent of Wilt Disease of Red Chili Plants Heriyati, Sukma; Prasetyawati, Endang Triwahyu; Purnawati, Arika
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.45386

Abstract

Wilt disease caused by Fusarium oxysporum is one of the diseases affecting chili plants. The utilization of Bacillus spp. can be a solution for plant disease control because it can colonize plants and produce useful microbe compounds to inhibit the development of plant pathogen. This study aimed to determine the ability of Bacillus spp. to suppress the growth of Fusarium sp. isolates from chili plants in vitro. Bacillus sp. isolates Ba-6, Ba-9, Ba-12, Ba-15, Ba-17, and a control (aquadest) were used as treatments, each replicated four times. The observation parameters were the inhibition zone test and microscopic observations of the morphology of Fusarium sp. after the inhibition zone test. The highest inhibition was shown in the treatment of Bacillus sp. isolate Ba-15, which was 20.02%. The observation of Fusarium sp. hyphae after the inhibition zone test showed abnormal growth, which was different for each isolate. Some hyphae were bent, coiled, shrunken, swollen, curled, or lysed.
Eficacy of Paenibacillus sp. Using seed treatment for controlling Bacterial Leaf Blight Caused by Xanthomonas sp. in Rice Plants Al Rahmad, Nirmala Putri; Purnawati, Arika; Prasetyawati, Endang Triwahyu
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.65040

Abstract

Rice (Oryza sativa L.) is one of the important food commodities consumed by the Indonesian people. One of the reasons for the decline in rice production is infection by the pathogen Xanthomonas oryzae pv. oryzae (Xoo) which causes bacterial leaf blight (BLB) and it’s seed-borne pathogen. The purpose of the experiment is to to evaluate the effectiveness of seed soaking using Paenibacillus sp. to control BLB in rice plants. Efforts to control BLB and improve rice plant growth were carried out by seed treatment using biocontrol agent has potential as biological control for BLB in rice plants by producing antibiotic. This experiment used a Completely Randomized Design with 4 treatments (control, Paenibacillus sp., Bacillus sp. BTH22, Paenibacillus polymyxa) and 5 replications. Data analysis was performed using analysis of variance (ANOVA) at a 5% significance level using IBM SPSS Statistics version 22 software. If the analysis results showed significant differences between treatments, an Honestly Significant Difference (HSD) test was performed at the 5% level. The research results showed that seed soaking using Paenibacillus sp. was able to reduce the incubation period of HDB disease by up to 11 days, with the lowest disease attack intensity being 10.21%, the highest seed germination rate being 91.25%, and an efficacy level of 53.32%.
Consortium of Bacillus sp. BTH22 and Trichoderma sp. to Control Bacterial Leaf Blight Disease in Rice Plants Dwirizki Permata, Aprilla Hendiana; Purnawati, Arika; Prasetyawati, Endang Triwahyu
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.65958

Abstract

Rice is a major food commodity in Indonesia that is currently experiencing a decline in production due to several factors, one of which is bacterial leaf blight (BLB) caused by the bacterium Xanthomonas sp. The use of chemicals in controlling this disease has many limitations, so alternatives are needed, such as antagonistic microbes. This study aims to evaluate the effectiveness of Bacillus sp. BTH22 and Trichoderma sp. individually and in consortium in suppressing BLB disease in rice plants in vitro and in vivo. The study was conducted at the Plant Health Laboratory of  Universitas Pembangunan Nasional “Veteran” Jawa Timur and Green House of Kebun Bibit Wonorejo from March to June 2025. The methods used included in vitro diffusion well test and in vivo disease intensity observation with a completely randomized design consisting of 5 treatments, namely control and spraying of antagonistic microbes Bacillus sp. BTH22 and Trichoderma sp. alone or in consortium with 4 replicates. Data analysis used analysis of variance (ANOVA) and post-hoc HSD tests at a 5% significance level. The results show that the treatment with the Bacillus sp. BTH22 and Trichoderma sp. consortium was the most effective, with an inhibition zone of 13 mm, which is classified as strong, and the lowest disease intensity of 30.02% at 55 HST with a suppression rate of 69.98%. These results indicate that the consortium of the two antagonistic microorganisms has high potential as an effective and sustainable biological control agent for BLB based on the observed parameters of inhibition zone and disease attack intensity.
Bioencapsulation of Streptomyces sp. with Sodium Alginate for Control Fusarium sp. in Shallot Plants Widyananda, Kanaya Syafina; Purnawati, Arika; Wiyatiningsih, Sri
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.67882

Abstract

This study investigated the effects of Streptomyces sp. encapsulated in sodium alginate beads on the growth and health of shallot (Allium cepa L.) plants. Different sodium alginate concentrations (2%, 2.5%, and 3%) and application timings (7 days before transplanting and at transplanting) were tested. The mass and diameter of the beads showed slight decreases over storage time, indicating some shrinkage but maintaining structural integrity to protect the bacteria. Encapsulation efficiency was highest at 3% sodium alginate, which also correlated    with    increased    bead     stability.     Viability     tests     revealed that Streptomyces maintained high colony-forming units (CFU) across the treatments, confirming the protective effect of the encapsulation. Application of beads significantly reduced Fusarium wilt disease intensity and improved bulb weight. Treatments with higher sodium alginate concentrations and earlier application times showed the most pronounced benefits. These findings demonstrate that beads using optimal sodium alginate concentration and timing enhance microbial viability and biological control efficacy, contributing to healthier and more productive shallot cultivation.
Isolation and Identification of Indigenous Bacteria Potential to Degradate Mancozeb from Shallots (Allium cepa L.) Cultivation Land in Nganjuk District Khairunnisa, Nabila Zahra; Prasetyawati, Endang Triwahyu; Purnawati, Arika
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.67683

Abstract

The use of a fungicide containing the active ingredient mancozeb in shallot fields to control moler disease, if not managed in terms of dose and frequency, it has the potential to harm soil in physical, chemical and biological ways. One method for repairing this damage is too use local microbes. This research attempts to determine the types of bacteria in soil treated to mancozeb fungicide through a morphological and physiological characterization process. Soil samples were collected from the shallot producing region of Balongrejo District, Nganjuk Regency. The method in this research is to isolate soil samples at a depth of 0-20 cm. Bacteria were isolated and multiplied using the streak plate technique on nutrient agar medium. Identification was performed on macroscopic and microscopic morphological features well as physiological characters based on the method of Schaad et al. (2001). Bergey's Manual of Determinative Bacteriology Ninth Edition was then used to identify the isolated bacteria. The research results showed that 14 bacterial isolates were isolated, 5 isolates suspected to be the genus Bacillus, 7 isolates suspected to be the genus Corynebacterium, 1 isolate suspected to be Lactobacillus, and 1 isolate unidentified. All indigenous bacterial isolates were able to reduce the concentration of mancozeb fungicide through measurements using a UV-Vis spechtrophotometer with a wavelength of 268 nm. The highest degradation percentage was bacterial isolate I8 (75%) and the lowest was bacterial isolate I7 (19%).
Co-Authors Aditya, Haidar Fari Ahmad Adibul Akrom Akrom, Ahmad Adibul Al Rahmad, Nirmala Putri Alfa, Maria Rodhya Althaf, Marcellino Alvia Cindy Apriliani Amalia, Anisa Rizki Aris Cahyono Bambang HADISUTRISNO Diasi, Ananta Rizki Didik Utomo Pribadi Dwirizki Permata, Aprilla Hendiana Elly Syafriani Endang Triwahyu Prasetyawati Fari A, Haidar Fatimah Nabila Zahra Firmansyah, Moch Ryan Hadi Suhardjono Hasni Wardahni Haykal, Arkan Heriyati, Sukma Herry Nirwanto Iis Purnamawati Ika Noviyanti, Rizky Indah Pemuda Indarwati Indarwati Irsyad Irsyad Ketut Srie Marhaeni Julyasih Khairunnisa, Nabila Zahra Kurniawati Kurniawati Laras Setyowati Laras Setyowati Lestari, Safira Rizka Maharani, Salsabilla Diva Mahfud, Nur Aini Marcellino Althaf Maria Rodhya Alfa Maulina Aizah, Shinta Mujoko , Tri Mukaromah, Fathul Nensi Agustina Nensi Agustina Nensi Agustina Nijma Nurfadila Noni Rahmadhini Nora Augustien Novi Nafisah Najmi Nur Aini Mahfud Pawana Nur Indah Penta SURYAMINARSIH Prasetyawati, Endang Triwahyu Prasetyowati, Endang Triwahyu Presti Mardiyani Rizky Ika Noviyanti Saefur Rohman Safira Rizka Lestari Sayekti, Ninik Ari Setyowati, Laras Sri Wiyatiningsih Syamsul Arifin Tri Mudjoko Tri MUJOKO Triwahyu P, Endang Triwidodo ARWIYANTO Widayati, Wilujeng Widyananda, Kanaya Syafina Wilujeng Widayati Windriyanti, Wiwin Wiwik Harjani Yanti, Vika Yenny WURYANDARI Zakaria Wildan Fachrezzy Zakqy, Nauval