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All Journal Natural Science: Journal of Science and Technology Agrotrop : Journal on Agriculture Science BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Pelita Perkebunan Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Penelitian Hayati Jurnal Kreativitas dan Inovasi (Jurnal Kreanova)
Edy Setiti Wida Utami
Biology Department, Faculty Of Sciences And Technology, Airlangga University, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN Edy Setiti Wida Utami; Issirep Soemardi; Taryono Taryono; Endang Semiarti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 1 (2007): December 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/320

Abstract

Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L) Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM) e.i.: NP(New Phalaenopsis) medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium) e.i.: NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM). The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.
PENGKAJIAN BERBAGAI KONSENTRASI RAGI ROTI UNTUK PERTUMBUHAN PLANLET TEBU Saccharum spp var PS 61 IN VITRO Edy Setiti Wida Utami; Dwi Setyo Rini; Eka Sugiyarta
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 2 (2001): June 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/499

Abstract

This experiment was expect for the find of alternative substitution of dalapon’s herbicide, its have active materials as 2,2 dichloropropionat acid. The fuction of dichloropropionat is very important on the differentiation and growing of sugarcane plantlet. The purpose of the research was to know the effect some concentration of Baker’s yeast that consist Saccharomyces cereviceae to growth of sugarcane (Saccharum spp) var PS61 plantlet. This experiment was arranged in a Completely Randomized Design with eight treatments and four replications. The treatment were: (K) control; (Po) without dalapon’s herbicide and Baker’s yeast; (0.25 g/l) medium; Bakers yeast 0.5 g/l medium; (P0.75) g/l medium; (P1.0) Baker’s yeast 1.0 g/l medium; (P1.25) Baker’s yeast 1.25 g/l medium; (P1.5) Baker’s yeast 1.5 g/l medium. The observation was done by destruction of the media every two weeks until the twelve weeks to the height of shoots, and length of primary roots. The result of this experiment showed that the application of Baker’s yeast media could be increasing the heigh of shoots and the length of primary roots. Concentration of Baker’s yeast that the suitable for growing of the sugarcane plantlet is 0.25 mg/l.
Callus Induction of Piper betle L. Var Nigra Using 2,4-Dichlorofenoxyacetic Acidand 6-Benzil Aminopurin Junairiah Junairiah; Purnomo Purnomo; Edy Setiti Wida Utami; Ni’matuzahroh Ni’matuzahroh; Lilis Sulistyorini
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.15962

Abstract

Piper betle L var Nigra (black betel) is a member of Piperaceae family which has potential as medicinal plant due to its secondary metabolites. Callus culture is one of the alternative methods to elevate production of secondary metabolites. This study was aimed to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzil aminopurine (BAP) towards callus induction and growth, also to determine the most optimal variation of 2,4-D and BAP concentration for callus induction of black betel leaf explant. This study was a laboratory experimental study with complete randomized design. Black betel leaf explant was planted in Murashige and Skoog (MS) medium supplemented with 2,4-D and BAP growth regulators at concentration of 0.0 mg/L, 0.5 mg/L, 1.0 mg/L, 1.5 mg/L, 2.0 mg/L respectively. Parameter recorded for callus induction and growth including callus induction time (days), percentage of explant forming callus, fresh weight, dry weight, color and texture. After callus planted for 8 weeks, analysis was performed statistically. Result showed that 2,4-D and BAP supplementation to medium affected the growth of black bete l leaf explants. Additional concentration of 0.5 mg/L 2,4-D and 1.0 mg/L BAP growth regulators showed the fastest response in callus formation, at 7.25 days. Growth regulators of 2,4-D 0.5 mg/L and BAP 2.0 mg/L concentration produced the highest fresh and dry weight, at 0.6802 g and 0.0670 g respectively. The best treatment was used as a basis to produce secondary metabolites.
Callus Induction of Gendarussa (Justicia gendarussa) by Various Concentration of 2,4-D, IBA, and BAP Dwi Kusuma Wahyuni; Putri Andriani; Arif Nur Muhammad Ansori; Edy Setiti Wida Utami
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11347

Abstract

Justicia gendarussa Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (α = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. Moreover, the optimum treatment will be used to produce secondary metabolite and seed synthetic by cell suspension culture.
The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro Edy Setiti Wida Utami; Sucipto Hariyanto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5165

Abstract

Dendrobium antennatum has high economic value as cut flowers and flowerpots. Like orchid seeds in general, D. antennatum is difficult to germinate under natural conditions. This study aimed to determine the effect of coconut water on seed germination and embryo development, as well as the effect of NAA on shoots development of D. antennatum. This study consisted of two stages. In the first stage, the 12 weeks-old seeds after pollination were sown on MS medium containing 2 g/L peptone + 0%; 5%; 10%; and 20% coconut water. After 8 weeks of culture, the seeds germinated and the shoot formed were recorded. The highest in seed germination (92.2%) and the formation of shoots (51.4%) were obtained when seeds were cultured on MS medium containing 2 g/L peptone + 20% coconut water. In the second stage, the shoots were sub-cultured on MS medium containing 1 mg/L thidiazuron + 0 mg/L; 1 mg/L; 2 mg/L; and 3 mg/L NAA. After 16 weeks of sub-culture, the height of plantlets, the length of the roots and leaves, number of leaves and roots formed were recorded. MS medium containing 1 mg/L thidiazuron + 1 mg/L NAA was the most suitable for the shoots development of D.antennatum. The embryo development of D.antennatum in vitro begins with the enlargement of embryo, with further it emerges from the seed coat (germinated) followed by the formation of the apical meristems to form the shoots and the roots.How to CiteUtami, E. S. W., Hariyanto, S. (2016). The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro. Biosaintifika: Journal of Biology Biology Education, 8(2), 165-171.
Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510 Dwi Kusuma Wahyuni; Siti Nur Hafida; Tri Muji Ermayanti; Bambang Prajoga Eko Wardoyo; Hery Purnobasuki; Edy Setiti Wida Utami
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8223

Abstract

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be  available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction. 
Optimation of Auxin and Cytokinin on Enhanced Quality and Weight of Coffea liberica Somatic Embryos Fitria Ardiyani; Edy Setiti Wida Utami; Hery Purnobasuki
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 37 No 1 (2021)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v37i1.460

Abstract

Coffea liberica is a variety of coffee that tolerant to marginal land, especially peatlands. One of propagation methods in C. liberica is somatic embryogenesis(SE) which producing large number of true-to-type plant seedlings in a short time. This research aimed at studying the effect of application of plant growthregulator (PGR) on quality and weight of somatic embryo of C. liberica. Somatic embryo in development stage was induced by Murashige and Skoog medium containing cytokinin as benzyl amino purin (BAP) and auxin as 2,4-dichlorophe-noxyacetic acid (2,4-D). While cotyledonary embryo in germination stage was induced by Murashige and Skoog medium containing cytokinin (BAP) and auxins as 2,4-D, indole acetic acid (IAA) and naphthaleneacetic acid (NAA). The resultsshowed that the application of auxins and cytokinins on development stage affected the formation of embryos, texture of calli, color of calli and embryos, and weight of somatic embryo. It also influenced the shoot and root formation, color and weight of geminating embryos of C. liberica at the germinating stage. During the development stage, addition of 1 mg/L BAP in the absence of 2,4-D in MS medium produced the highest quality of somatic embryo of C. liberica. This medium also produced heaviest somatic embryos but with lighter callus. While in germination stage, all medium treatments produced a typical germinating embryo. Coffea liberica germinating embryo growth optimally on MS medium containing 0.5 mg/L BAP as a single chemical or 0.5 mg/L BAP in combination with 0.5 mg/L IAA for shooting development. Whereas on rooting development, addition of 0.5 mg/L NAA on MS medium produced an optimal germinating embryo. Moreover, germination embryo of C. liberica recorded the highest in terms of dry weight on MS media with addition of 0.5 mg/L BAP. Application of appropriate concentration of auxin and cytokinin is needed to support the formation of somatic embryo and germinating embryo.
PELATIHAN BUDIDAYA ANGGREK SECARA IN VITRO PADA KELOMPOK TANI ANGGREK “SANDERIANA” Wahyuni, Dwi Kusuma; Utami, Edy Setiti Wida; Hamidah, Hamidah; Purnobasuki, Hery; Junairiah, Junairiah
Jurnal Kreativitas dan Inovasi (Jurnal Kreanova) Vol 5 No 1 (2025)
Publisher : Sekolah Tinggi Ilmu Ekonomi Indonesia (STIESIA) Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24034/kreanova.v5i1.6924

Abstract

Indonesia sebagai negara megabiodiversitas mempunyai jenis-jenis anggrek yang melimpah. Pengambilan langsung dari alam membuat banyak jenis anggrek yang masuk dalam daftar CITES (Convention On International Trade In Endangered Species Of Wild Fauna and Flora), Apendiks 1. Salah satu upaya pelestarian anggrek adalah perbanyakan melalui metode kultur jaringan. Departemen Biologi, Fakultas Sains dan Teknologi Universitas Airlangga telah melakukan banyak penelitian tentang budidaya anggrek dengan kultur jaringan. Untuk menyebarluaskan hasil penelitian tersebut agar bermanfaat untuk masyarakat luas, telah dilakukan kegiatan pengabdian masyarakat berupa pelatihan budidaya anggrek untuk kelompok tani anggrek “Sanderiana” di Dadaprejo, Junrejo, Batu, Jawa timur yang berkerjasama dengan DD Orchid dan Taman Husada Graha Famili. Pelatihan difokuskan di Malang karena Malang merupakan sentra kegiatan pertanian anggrek nasional. Pelatihan budidaya nggrek juga difokuskan pada teknik kultur jaringan anggrek untuk anggrek langka sebagai upaya pelestarian dan juga untuk meningkatkan perekonomian petani anggrek. Kegiatan pelatihan dilakukan selama 6 bulan, meliputi tahap persiapan, pelatihan dan workshop, evaluasi 1 dan evalusi 2. Teknik budidaya yang dilakukan adalah budidaya kloning anggrek secara in vitro.
Seed Germination of Avicennia marina (Forsk.) Vierh. By Pericarp Removal Treatment Purnobasuki, Hery; Utami, Edy Setiti Wida
BIOTROPIA Vol. 23 No. 2 (2016): BIOTROPIA Vol. 23 No. 2 December 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2016.23.2.349

Abstract

Avicennia marina is one species used for building coastal ecosystem stabilization on the Surabaya shores because of its tolerance to high salinity levels and resistance to wave energy. Its existence is threatened by various factors, including land conversion, pollution, and the construction of inter-island bridges. The growth of A. marina is currently limited by seed dormancy. To increase propagation efficiency, seed viability and vigor were compared based on collection dates and pericarp removal methods. The results showed no relationship between the salinity levels of soaking water or seed buoyancy and seed viability or vigor. Collection dates may influence seed viability and vigor, as seeds collected in October and November germinated more quickly than those collected in December, although there were no differences in total germination. Hand-peeled seeds germinated more slowly than soaked seeds for all collection dates, but with no differences in total germination. Seeds collected in October and November grew more vigorously than those collected in December.
Co-Authors Aldy Bahaduri Indraloka Arif Nur Muhammad Ansori Arif Yachya Bambang Prajoga Eko Wardoyo Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Diah Ayu Wulandari Dwi Kusuma Wahyuni Dwi Setyo Rini Eka Sugiyarta Endang Semiarti Fitria Ardiyani Hamidah . Hery Purnobasuki Issirep Soemardi Junairiah Junairiah Lilis Sulistyorini Nabilah Istighfari Zuraidassanaaz Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh, Ni’matuzahroh Purnomo Purnomo Purnomo Purnomo Putri Andriani Putri Andriani Shilfiana Rahayu Siti Nur Hafida Siti Nur Hafida, Siti Nur Sucipto Hariyanto Taryono Taryono Tri Muji Ermayanti Tri Muji Ermayanti Y. SRI WULAN MANUHARA Y. Sri. Wulan Manuhara