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All Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Indian Journal of Forensic Medicine & Toxicology Public Health Research Development
Puspa Wardhani Puspa Wardhani
Stmik Jakarta
Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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ANTI DENGUE IGG/IGM RATIO FOR SECONDARY ADULT DENGUE INFECTION IN SURABAYA Aryati Aryati; Puspa Wardhani; Ade Rochaeni; Jeine Stela Akualing; Usman Hadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 1 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i1.1161

Abstract

Infeksi Virus Dengue (IVD) dibedakan menjadi infeksi primer dan sekunder berdasarkan respons antibodi yang dihasilkan. Infeksisekunder perlu dibedakan dari infeksi primer karena umumnya menimbulkan manifestasi klinis yang berat. Uji hemaglutinasi inhibisisebagai baku emas untuk menentukan infeksi primer atau sekunder dirasa tidak praktis karena membutuhkan sepasang sera denganselang waktu waktu yang cukup lama. Penelitian ini bertujuan mengetahui cut-off rasio IgG/IgM anti dengue untuk infeksi denguesekunder dewasa di Surabaya. Subjek adalah pasien IVD dengan hasil NS1 dan/atau PCR dengue positif. Rasio IgG/IgM anti-denguediperoleh dari pembagian nilai indeks IgG dan IgM metode ELISA. Nilai cut-off rasio ditentukan berdasarkan kurva ROC. Berdasarkanpola reaktivitas IgM dan IgG ELISA, 19 (31,1%) pasien dikelompokkan sebagai infeksi primer dan 42 (68,9%) infeksi sekunder. HasilPCR didominasi DEN-3. Nilai cut-off optimal rasio IgG/IgM ≥0,927 sebagai peramal infeksi sekunder memiliki kepekaan 66,7% dankekhasan 63,2%. Dianalisis pula nilai cut-off optimal IgM dan IgG anti dengue, yaitu IgM ≥1,515 dan IgG ≥2,034 sebagai peramalinfeksi sekunder memiliki kepekaan dan kekhasans masing-masing 85,7% dan 84,2%; 100% dan 100%. Disimpulkan bahwa rasioIgG/IgM ≥0,927 tidak dapat digunakan sebagai tolok ukur tunggal peramal infeksi sekunder sedangkan cut-off IgG ≥2,034 dapatdipertimbangkan sebagai peramal infeksi sekunder.
APPLICATION OF DNA METHYLATION ON URINE SAMPLE FOR AGE ESTIMATION (Penggunaan Metilasi DNA dalam Perkiraan Umur Individu di Sampel Air Kemih) Rosalinda Avia Eryatma; Puspa Wardhani; Ahmad Yudianto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1179

Abstract

Perkiraan umur sangat penting dalam analisis forensik. Umur individu lebih sering diperkirakan dengan menggunakan tulangdan gigi. Namun, hanya terbatas pada kasus tertentu yang berhubungan dengan kerangka manusia. Metilasi DNA merupakan salahsatu cara dalam memperkirakan umur di sampel biologis. Perkiraan umur menggunakan metode metilasi DNA dengan penggunaansampel air kemih hingga saat ini belum pernah ada yang melakukan, oleh sebab itu penelitian ini akan mengunakan isolasi metilasiDNA dalam memperkirakan umur individu di sampel airkemih. Di penelitian ini digunakan 6 sampel air kemih yang didapatkan daripendonor sehat. Tahap pertama adalah isolasi DNA dengan menggunakan DNAzol dan kloroform setelah itu, dikonversi bisulfit dengankit DNA metilasi. Hasil isolasi kemudian di amplifikasi dengan metode PCR dan di elektroforesis dengan gel agarosa. Hasil elektroforesisdapat sebagai acuan panjang pita yang di sekuensing. Hasil sekuensing dianalisis persen metilasinya dan korelasinya dengan perkiraanumur. Hasil dari pembacaan aplikasi dan perhitungan tersebut di sampel 001 menunjukkan 64,99%, sampel 002 menunjukkan 69,45%,sampel 003 menunjukkan 57,52%, sampel 4 menunjukkan 58,61%, sampel 005 menunjukkan 63,66% dan sampel 006 menunjukkan61,19%. Berdasarkan hasil penelitian ini merupakan langkah awal dalam penggunaan metilasi DNA dalam perkiraan umur individu terutamadi sampel air kemih.
CORRELATION OF NEUTROPHILS/LYMPHOCTES RATIO AND C-REACTIVE PROTEIN IN SEPSIS PATIENTS Henny Elfira Yanti; Fery H Soedewo; Puspa Wardhani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1143

Abstract

Sepsis merupakan penyebab umum dari kesakitan dan kematian di seluruh dunia. Diagnosis yang cepat dan tepat sangatdiperlukan. Salah satu respons fisiologis pada sistem imunitas terhadap inflamasi sistemik adalah peningkatan jumlah neutrofil danpenurunan jumlah limfosit. Rasio neutrofil/limfosit telah diketahui sebagai petanda inflamasi yang cepat, sederhana dan murah. Tujuanpenelitian ini adalah membuktikan adanya kenasaban antara rasio neutrofil/limfosit dan c-reactive protein di pasien sepsis. Penelitianbersifat ptong lintang observasional, dilakukan masa waktu Januari hingga Juni 2015. Sampel penelitian terdiri dari 42 pasien sepsisyang dirawat di Instalasi Rawat Darurat RSUD Dr. Soetomo Surabaya. Semua sampel dilakukan pemeriksaan jumlah limfosit, neutrofil,CRP. Hasil dianalisis menggunakan uji kenasaban Spearman’s. Rasio neutrofil/limfosit berkisar antara 3,42–57,47 (21,74±14,1). KadarCRP antara 1,22 mg/L–361,86 mg/L (158 mg/L±97,4 mg/L). Hasil penelitian tidak terdapat kenasaban antara RNL dan kadar CRPdi pasien sepsis (p=0,51). Tidak terdapat kenasaban antara RNL dan kadar CRP di pasien sepsis. Hal ini disebabkan adanya perbedaanjalur aktivasi antara neutrofil dengan CRP sehingga menyebabkan peningkatan CRP tidak sebanding dengan peningkatan jumlahneutrofil.
COMPARISON RESULTS OF ANALYTICAL PROFILE INDEX AND DISC DIFFUSION ANTIMICROBIAL SUSCEPTIBILITY TEST TO TECHNICAL DEDICATED REASONABLE 300B METHOD IG Eka Sugiartha; Bambang Pujo Semedi; Puspa Wardhani; IGAA Putri Sri Rejeki
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1134

Abstract

Angka kematian infeksi aliran darah cukup tinggi, berkisar 20–50%. Patogen penyebab dapat dibuktikan dengan pemeriksaan kulturdarah yang dilanjutkan dengan uji kepekaan antibiotika. Metode pemeriksaan dapat dilakukan secara manual atau automatis baiksemiautomatis ataupun automatis penuh. Metode manual relatif tidak memerlukan biaya yang besar dibandingkan metode automatisasi.Penelitian ini merupakan analisis observasional dengan desain potong lintang. Metode identifikasi manual memakai metode API danuji kepekaan antibiotika metode difusi cakram antibiotika Kirby Bauer. Kedua metode ini dibandingkan dengan metode semiautomatisTDR-300B. Metode automatis penuh VITEK 2 digunakan sebagai metode rujukan untuk menilai kinerja metode konvensional dansemiautomatis. Bakteri penyebab infeksi aliran darah didominasi Gram negatif kebanyakan Eschericia coli dan Klebsiella pneumonia.Ketepatan metode identifikasi API terhadap VITEK 2 sebesar 87,87%, ketepatan identifikasi metode TDR-300B terhadap metode VITEK2 adalah 90,9%. Hasil ketepatan uji kepekaan antibiotika metode konvensional difusi cakram antibiotika Kirby Bauer terhadap metodeVITEK 2 adalah 84,64%. Ketepatan uji kepekaan antibiotika metode TDR-300B terhadap metode VITEK 2 sebesar 82,5%. Ketepatanmetode API terhadap metode TDR-300B sebesar 84,84%. Ketepatan uji kepekaan antibiotika metode konvensional terhadap metodeTDR-300B sebesar 78,21%. Hasil metode identifikasi dan uji kepekaan antibiotika konvensional tidak berbeda bermakna secara statistikdengan metode semiautomatis TDR-300B. Metode identifikasi dan uji kepekaan antibiotika konvensional masih dapat dipercaya terutamauntuk daerah dengan keterbatasan biaya atau pemeriksaan masih sedikit.
IMMATURE PLATELET FRACTION DI DEMAM DENGUE DAN DEMAM BERDARAH DENGUE (Immature Platelet Fraction in Dengue Fever and Dengue Hemorrhagic Fever) Izzuki Muhashonah; Juli Soemarsono; Puspa Wardhani; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1257

Abstract

Thrombocytopenia is a hematological abnormality found in the majority of Dengue Virus Infection cases with manifestations suchas Dengue Fever (DF) and Dengue Hemorrhagic Fever (DHF). Bone marrow response to the decrease in platelets is by increasingthrombopoiesis which can be identified by Immature Platelet Fraction (IPF) examination as an indirect indicator of bone marrow responseto thrombocytopenia. The examination of IPF in venous blood was performed on 29 subjects who met the 1997 WHO criteria, carriedout from January until August 2012. The EDTA blood samples were examined twice, on the day of their admittance and two days later,based on a flowcytometry principle using Sysmex XE-2100. The IPF was derived from the immature platelet ratio against the total numberof platelets (IPF %). The test results were statistically analyzed by using SPSS 20. It was found, that IPF in DHF compared between thefirst and the third day of their admittance was statistically significantly different with p = 0.033 compared to DF with p = 0.444. ThePearson’s correlation showed an inverse correlation between IPF and platelets with r = -0.675 and p = 0.01. The statistical analysisrevealed a significant difference in IPF between moderate- and mild-thrombocytopenia on the first and third day of their admittance withp = 0.014 and 0.001, respectively. Based on this study it can be concluded that IPF can be used to indicate the bone marrow response inboth DF and DHF related to thrombocytopenia.
The differences of parasitemia in Plasmodium berghei infected mice treated with extract of mango parasite leaves with Artemisinin combination Muhammad Zulkifly Tasman; Heny Arwati; Nurina Hasanatuludhhiyah; Puspa Wardhani
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 7 No 1 (2023): Qanun Medika Vol 07 No 01 January 2023
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v7i1.13882

Abstract

Malaria is a disease transmitted through the bite of a female Anopheles mosquito caused by the Plasmodium parasite. Plasmodium has been reported to become resistant to artemisinin. Thus, the study on the ethanol extract of mango parasite leaves/Dendrophthoe pentandra (MP) on P. berghei-infected BALB/c mice with and without artemisinin combination (MP+A) was conducted. This study is experimental laboratory research with a post-test only design. The percent parasitemia in mice treated with ethanol extract of MP and MP+A leaves decreased. There was no difference in the spleen index in mice given ethanol extract of MP, and MP+A leaves with p = 0.203 and the spleen weight of mice with p = 0.134 (significance: p <0.005). Pearson Correlation test showed a correlation between spleen index with parasitemia and spleen weight with parasitemia; however, there was no correlation between body weight and parasitemia. The ethanol extracts of MP and MP+A leaves had significant antimalarial activity, and the difference in the percent parasitemia between groups was significant but not so far. The spleen index value was not affected by the ethanol extract of MP and MP+A leaves, but the percent parasitemia was affected.
Lupus Nephritis: A Literature Review Bestri Fortuna; Awalia; Puspa Wardhani
Current Internal Medicine Research and Practice Surabaya Journal Vol. 5 No. 1 (2024): CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cimrj.v5i1.52982

Abstract

Systemic lupus erythematosus (SLE) has diverse clinical presentations, including lupus nephritis (LN), if LN manifest the mortality & morbidity will be elevated. The majority of SLE cases found in females, especially during puberty and childbearing age. A comprehensive understanding of the epidemiology, classification, diagnosis, and management of LN is essential for medical practitioners. Initially, the diagnostic process requires clinical examinations to find clinical manifestations. This process then followed by laboratory assessments to diagnose SLE and identify any indication related to kidney damage, commonly proteinuria. Other findings such as haematuria and leukocyturia may also present. Antinuclear antibodies (ANA) test and anti-dsDNA serves as primary diagnostic tool. In addition, kidney biopsy is the gold standard for LN due to its ability to confirm and measure the stadium for treatment guide. The therapy is specifically based on clinical and biopsy findings, categorized by World Health Organization (WHO) into five classes, namely normal, mesangial, focal and segmental proliferative, diffuse proliferative, and membranous. Accurate diagnosis in the initial stage and suitable therapy is crucial to significantly improve the prognosis of LN. In this review, we address some clinical manifestations related to LN and standardised diagnostic assessment tools, with an ultimate goal to improve the outcome of patients with lupus through an individual-specific management.
Home Sanitation and Personal Hygiene as Risk Factors for Leprosy Incidents in Guluk-Guluk District, Madura Nur Laily Rizki Fajariyah; Imam Thohari; Marlik; Irwan Sulistio; Puspa Wardhani
Public Health Research Development Vol. 1 No. 1 (2024): Public Health Research Development
Publisher : Indonesian Association Environmental Health of West Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/phrd.v1i1.4

Abstract

Leprosy is still a health problem in the Guluk-Guluk District. Factors that contribute to the increase in leprosy cases are home sanitation and personal hygiene. House sanitation that does not meet the requirements creates a conducive environment for leprosy bacteria, and inadequate personal hygiene increases the risk of leprosy transmission. This research aims to analyze the relationship between home sanitation and personal hygiene and the incidence of leprosy. This research uses a case-control design. The independent variables are home sanitation and personal hygiene, while the dependent variable is the incidence of leprosy. Data collection is done through interviews, observation, and measurement. The samples used were 30 case samples and 30 control samples. The research location is in the Guluk-Guluk Health Center Working Area. The research time is January - June 2024. Sampling used a simple random sampling technique via lottery. Data were analyzed using chi-square and odds ratio tests. The results show a relationship between house sanitation, ceilings, floors, ventilation, personal hygiene, cleanliness of hands and feet, and the incidence of leprosy. There is no relationship between temperature, humidity, and bed cleanliness with the incidence of leprosy.
Co-Authors Ade Rochaeni Ahmad Yudianto Aryati aryati . Aryati Aryati atika Awalia Bestri Fortuna Erik Hasudungan Erwin Triyono Fery H Soedewo Henny Elfira Yanti Heny Arwati IG Eka Sugiartha IGAA Putri Sri Rejeki Imam Thohari Irwan Sulistio Izzuki Muhashonah Jeine Stela Akualing Juli Soemarsono M Faizi Marlik Muhammad Zulkifly Tasman Munawaroh Fitriyah Netty Harjantien Nur Laily Rizki Fajariyah Nurina Hasanatuludhhiyah Priangga Adi Wiratama Prihatini Prihatini Probohoesodo M.Y Puspitasari, Yessy Resna Resna Rosalinda Avia Eryatma Roudhotul Ismaillya Noor S Darmadi Semedi, Bambang Pujo Siti Munawaroh Syafarinah Nur Hidayah Akil Ummi Maimunah Usman Hadi