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All Journal Prosiding Conference on Research and Community Services Journal of Bio-molecule Research And Engineering
Ana Mariatul Khiftiyah
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Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Immunology & microbiology Mathematics Other

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PELATIHAN PEMBUATAN BAGLOG JAMUR BERBAHAN DASAR JERAMI UNTUK MEWUJUDKAN PERTANIAN ZERO WASTE Canggih Nailil Maghfiroh; Dyah Ayu Sri Hartanti; Yessita Puspaningrum; Ana Mariatul Khiftiyah
Prosiding Conference on Research and Community Services Vol 4, No 1 (2022): Fourth Prosiding Conference on Research and Community Services
Publisher : STKIP PGRI Jombang

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Abstract

Beras merupakan salah satu makanan  pokok masyarakat Indonesia, hal tersebut menyebabkan banyak lahan yang digunakan untuk budidaya tanaman padi. Sisa tanaman berupa jerami dibuang atau dibakar oleh para petani sehingga bisa menimbulkan pencemaran lingkungan. Solusi permasalahan tersebut yaitu pemanfaatan sebagai bahan baglog atau media tanam jamur. Jerami mengandung selulosa sehingga bisa dimanfaatkan sebagai media tanam jamur. Kandungan nutrisi dalam 100 gr jerami padi adalah selulosa 29,63%, kandungan hemiselulosa 17,11%, dan lignin 12,17%. Sasaran kegiatan pengabdian ini adalah Kelompok tani Tejo Selatan terletak  di Dusun Tejo Selatan, Desa Tejo, Kecamatan Mojoagung, Kabupaten Jombang. Lokasi berada di areal persawahan yang cukup strategis, dan banyak limbah tanaman berupa jerami. Berdasarkan hasil dari pengabdian yang telah dilakukan dapat disimpulkan bahwa limbah jerami bisa dimanfaatkan untuk baglog. Pengolahan untuk membuat jerami menjadi baglog dilakukan dengan cara fermentasi dan dilakukan sterilisasi.Penggunaan jerami sebagai baglog bisa mengurangi jumlah limbah pertanian sehingga bisa membantu mewujudkan pertanian zero waste
Hydrolytic Enzyme Producing Bacteria From The Gut Of Oryctes rhinoceros Larvae Inhabiting Household Waste Composer Ana Mariatul Khiftiyah; Ni'matuzahroh; Mochammad Affandi; Salamun; Silvia Kurnia Sari; Miftahul Jannah; Nastiti Trikurniadewi; Achmad Zainal Abidin; Nurul Wahyuni; Rizki Amaliah Zain; Dela Dwi Alawiyah; Brigita Nur Diyan Agustiana
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67564

Abstract

relatively shorter adult phase than the larval stage. The larval stage of this organism lives in piles of organic matter, including in piles of household waste. The ability of larvae to utilize organic matter in their environment is inseparable from the role of potential microbes that produce enzymes associated with their gut. This study aims to isolate, screen, and identify the potential bacteria in producing hydrolytic enzymes from gut of O. rhinoceros larvae. The third instar larvae were dissected aseptically to obtain gut bacterial isolates. The isolates were tested for their ability to produce amylase, cellulose, lipase, and protease using agar plate containing specific substrates. There were eight different isolates, and three of them had the ability to produce all four enzymes. The three isolates were identified as Bacillus. These results can be used as a reference for handling household organic waste.
Pycnoscelus surinamensis Endosymbiont Bacteria: Identification and its Amylase Characteristic Ni'matuzahroh; Fatimah; Sholikah; Ayu Tri Wahyuni; Tri Nurhariyati; Mochammad Affandi; Agus Supriyanto; Almando Geraldi; Tini Surtiningsih; Brigita Nur Diyan Agustiana; Dela Dwi Alawiyah; Ana Mariatul Khiftiyah; Silvia Kurnia Sari
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67571

Abstract

The amylase is an enzyme that widely used in industry. In this research, amylase was isolated from endosymbiont bacteria (EKA 4) of cockroach Pycnoscelus surinamensis from household garbage dump Surabaya. This research aims to identify bacteria EKA 4, produce and characterize amylase from it at various temperature, pH and effect of metal ions. Amylase screening assay was done by imoculating the isolate into medium contain 2% of starch then incubated for 24 hours. Detection of amylase was done qualitatively by measured presence of halozone and quantitatively using DNS method. 16S rRNA analysis found that bacteria EKA 4 has similarity to Bacillus subtilis with 100% of percent identity. Result showed that optimum activity of amylase from B. subtilis were at 40°C, pH 6 and could use ion CaCl2 as a cofactor in a reaction. Thus, utilization of amylase from endosymbiont P. surinamensis could be as an alternative source in big industries.
Co-Authors ACHMAD ZAINAL ABIDIN Affandi, Moch Agus Supriyanto Almando Geraldi Ayu Tri Wahyuni Brigita Nur Diyan Agustiana Canggih Nailil Maghfiroh Dela Dwi Alawiyah Dyah Ayu Sri Hartanti Fatimah MIFTAHUL JANNAH Nastiti Trikurniadewi Ni'matuzahroh NURUL WAHYUNI Rizki Amaliah Zain SALAMUN Sholikah Silvia Kurnia Sari Tini Surtiningsih Tri Nurhariyati Yessita Puspaningrum