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All Journal Jurnal Akuakultur Rawa Indonesia Jurnal Veteriner Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Perikanan Universitas Gadjah Mada Ilmu Perikanan Tropis Nusantara Jurnal Kedokteran Hewan Jurnal Perikanan

Gina Saptiani

Universitas Mulawarman

Author-ID : 5482580

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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UJI IN VITRO GABUNGAN EKSTRAK TANAMAN DARI KALIMANTAN, INDONESIA TERHADAP BAKTERI PATOGEN PADA IKAN NILA Esti Handayani Hardi; Gina Saptiani; Nurkadina Nurkadina; Irawan Wijaya Kusuma; Wiwin Suwinarti
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to evaluate the antibacterial activity of ethanol concoction (mixed extract) of three herbs plants, such as: Boesenbergia pandurata, Solanum ferox and Zingimber zerumbet against Aeromonas hydrophila, Pseudomonas sp. and both bacteria. The Plant extracts were obtained by using ethanol and the concentration of the extract was 600 mgL-1 of B. pandurata, 900 mgL-1 of S. ferox and 200 mgL-1 of Z. zerumbet. The inhibition zone was measured after 6, 12, 18, 24 h incubation at 30o C. The ratios of concoction S. ferox and B. pandurata (for 100 mL solution) were 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90. The same ratios were made on the concoction of S. ferox and Z. zerumbet. The concoctions of S. ferox and B. pandurata in the ratio of 50:50 and 60:40, and the concoction of S. ferox and Z. Zerumbet with ratio 60:40 had higher antibacterial activity against A. hydrophila single isolate compared to the other concoctions. Furthermore, the concoction extract of S. ferox and B. pandurate 50:50 and the combined of S. ferox and Z. zerumbet ratio 50:50 and 90:10 were the best combination to inhibit the growth of a single bacterium Pseudomonas sp. The combined S. ferox and B. pandurata ratio 50:50; 10:90 and S. ferox mixed with Z. zerumbet ratio 50:50 and 40:60 were the best combination against the combined bacteria between A. hydrophila and Pseudomonas sp. The conclusion of this research was the combined extract of S. ferox and B. pandurata and S. ferox with Z. zerumbet are effective to suppress the growth of single or combination of A. hydropila and Pseudomonas sp.

Patogenesitas Bakteri Enterobacteriaceae pada Ikan Zebra (Danio rerio) Sebagai Hewan Model Widyaningsih Rahayu; Esti Handayani Hardi; Gina Saptiani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Enterobacteriaceae are Gram negative bacteria contain endotoxin and exotoxins which arerequirements for pathogenic bacteria and act as opportunistic pathogens. The purpose of this research was to determine the ability of Enterobacteriaceae bacteria to infect zebrafish (Danio rerio) by observing anatomical pathology, mortality, time death and cumulative time of death. The method used in thisresearch is a completely randomized design method (CRD). This research was conducted in three stages, first preparation of zebrasfish as animal model by average size 3-5cm and reach three months old. Second, bacterial cultures from 10 species of Enterobacteriaceae were Escherichia coli 1, E. coli 2, Enterobactercloacae 1, Acinetobacter calcoaceticus, Enterobacter amnigenus 1, Enterobacter amnigenus 1, Escherichia coli 3, Pantoea spp., E. cloacae 1, E. cloacae 2, E. cloacae 3, cultured in Brain Heart Infused Broth (BHIB) and later media washed with Phospate Buffer Saline (PBS) 0.45%. The third stage was bacterial infection to zebrafish using immersion method with each treatment repeated 3 times and observed for 120 hours. The results showed 10 species that used caused death (mortality), the highest mortality in fish that infected with E. coli 2 and E. cloacae 1 with a percentage of 66.67% infected by E. coli 2 and E. cloacae 1. Anatomicalpathology in the external organs and internal organs showed post-infectious symptoms. Infection occurred with mean time to death of 12-86 hours, as long as the cumulative time of fish death caused by E. coli 2, A.cinetobacter calcoaceticus and E. cloacae 1 causing death from 24 hours after infection until the end ofobservation.

POTENSI Avicenia marina UNTUK MENGHAMBAT MICROBA DAN MELINDUNGI BENUR UDANG WINDU Gina Saptiani; Andi Noor Asikin; Fikri Ardhani; Esti Handayani Hardi
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The research aims to study potency of Avicennia marina leaf extract to inhibit pathogens in vitro and in vivo on post larvae of tiger prawn. The leaves we re chopped, dried and extracted with water solvents, sea water andethanol. In vitro inhibitory tests were performed using agar disc diffusion (ADD) and minimal inhibitory concentration (MIC) methods. The in vivo test on tiger prawn PL-8 was given by submersion, which then tested with Vibrio harveyi and Saprolegnia sp. The in vitro test showed A. marina extract can inhibit V.harveyi and Saprolegnia sp. In vivo tests of V. harveyi and Saprolegnia sp. infections, showed 60- 88% survival and 57.33-86.67%, with protective ability of the highest pathogen infection of 80.80% and 77.80%. The best A. marina extract that can inhibit microbial and protect the tiger prawn from pathogen infection is ethanol extract with concentration 1,250-1,500 ppm, followed by sea water extract 1,500 ppm and water extract 1,500 ppm respectively

Co-Authors , Agustina Agus Sabdono Agustina Agustina Agustina Agustina Agustina Agustina Aipassa, Marlon Ivanhoe Andi Noor Asikin Andre Jolpano Catur A Pebrianto Eko Budi Prasetyo Esti H Hardi Esti Handayani Hardi Feby Kristina Fikri Ardhani Ida Ayu Putu Sri Widnyani Irawan Wijaya Kusuma, Irawan Wijaya Kiswanto Kiswanto Nurkadina Nurkadina Puput Andriani Rahmi Nohon Salaho Dina Devy Slamet Budi Prayitno Sundari Hidayat Sutrisno Anggoro Suwinarti, Wiwin Taufan Purwokusumaning Daru, Taufan Purwokusumaning Widyaningsih Rahayu Yosep Ruslim

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