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KARAKTERISTIK ORGANOLEPTIK, SIFAT KIMIA DAN AKTIVITAS ANTIOKSIDAN TEH FORMULASI DAUN KAKAO DAN KAYU MANIS Sadimantara, Muhammad Syukri; asranudin, asranudin; Sadimantara, Fahria Nadiryati; Sakir, Sakir; suwarjoyowirayatno, suwarjoyowirayatno
Jurnal Sains dan Teknologi Pangan Vol 3, No 5 (2018): JURNAL SAINS DAN TEKNOLOGI PANGAN
Publisher : Jurnal Sains dan Teknologi Pangan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (25.875 KB)

Abstract

ABSTRACTThe aims of this study was to determine the effect of cocoa and cinnamon leaf formulations onorganoleptic assessment, chemical properties and antioxidant activity of cocoa leaves tea. This studyused a Completely Randomized Design (CRD), consisting of 5 types of cocoa and cinnamon leaveformulation namely 100% of cocoa leaves: 0% of cinnamon (KM0), 95% of cocoa leaves: 5% ocinnamon (KM1), 90% of leaf cacao: 10% of cinnamon (KM2), 85% of cocoa leaves: 15% of cinnamo(KM3) and 80% of cocoa leaves: 20% of cinnamon (KM4). The formulations of cocoa and cinnamoleaves had significant effect (p <0.05) on color and taste organoleptic tests, but was not significantleffect on aroma parameters. Based on organoleptic assessment, cocoa leaves tea can be accepteby panelists. Moisture content, pH, and antioxidant activity of selected sample were 3.96%, 5.12-6.2and 39.55%, respectively. Based on these results, cocoa leaves tea with the addition of cinnamon canmeet the standard.Keywords: Cocoa Leaf Tea, Cinnamon, Organoleptic, Antioxidant activities.
SIFAT FISIKO KIMIA TEPUNG SUWEG (Amorphophallus paeoniifolius) ASAL KEBUN RAYA UNIVERSITAS HALU OLEO Holilah, Holilah; Asranudin, Asranudin; Kholidha S, Andi Noor
Jurnal Sains dan Teknologi Pangan 2017: PROSIDING SEMINAR NASIONAL FKPT-TPI 2017
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

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Abstract

Amorphophallus paeoniifolius adalah jenis umbi-umbian yang melimpah di Sulawesi Tenggara.UmbiAmorphophallus paeoniifolius mengandung garam oksalat yang menyebabkan rasa gatal. Proses penepunganAmorphophallus paeoniifolius mengikuti tahap preparasi yaitu penguapasan, pengecilan ukuran, perendamandalam HCl encer, perendaman dalam natrium karbonat, pencucian, pengeringan dan penggilingan. Berdasarkananalisis XRD menunjukkan tepung Amorphophallus paeoniifolius mengandungan pati A. Analisis DSCmenunjukkan suhu gelatinisasi tepung Amorphophallus paeoniifolius adalah 120ᵒC. Sedangkan analisiskomponem kimia yang meliputi kadar air, abu, lemak, protein dan amilosa berturut-turut adalah 6,2%; 4,3%;0,75%, 6,2% dan 16%.   Kata kunci: Amorphophallus paeoniifolius, garam oksalat, gelatinisasi
KARAKTERISTIK ORGANOLEPTIK, SIFAT KIMIA DAN AKTIVITAS ANTIOKSIDAN TEH FORMULASI DAUN KAKAO DAN KAYU MANIS Sadimantara, Muhammad Syukri; Asranudin, Asranudin; sakir, sakir; Sadimantara, Fahria Nadiryati; Suwarjoyowirayatno, Suwarjoyowirayatno; Rhenyslawaty, Rhenyslawaty
Jurnal Sains dan Teknologi Pangan Vol 3, No 5 (2018): JURNAL SAINS DAN TEKNOLOGI PANGAN
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.766 KB) | DOI: 10.33772/jstp.v3i5.6164

Abstract

ABSTRACT The aims of this study was to determine the effect of cocoa and cinnamon leaf formulations on organoleptic assessment, chemical properties and antioxidant activity of cocoa leaves tea. This study used a Completely Randomized Design (CRD), consisting of 5 types of cocoa and cinnamon leaves formulation namely 100% of cocoa leaves: 0% of cinnamon (KM0), 95% of cocoa leaves: 5% of cinnamon (KM1), 90% of leaf cacao: 10% of cinnamon (KM2), 85% of cocoa leaves: 15% of cinnamon  (KM3) and 80% of cocoa leaves: 20% of cinnamon  (KM4). The formulations of cocoa and cinnamon leaves had significant effect (p <0.05) on color and taste organoleptic tests, but was not significantly effect on aroma parameters. Based on organoleptic assessment, cocoa leaves tea can be accepted by panelists. Moisture content, pH, and antioxidant activity of selected sample were 3.96%, 5.12-6.20 and 39.55%, respectively. Based on these results, cocoa leaves tea with the addition of cinnamon can meet the standard. Keywords: Cocoa Leaf Tea, Cinnamon, Organoleptic, Antioxidant activities. ABSTRAK Tujuan  penelitian  adalah  untuk  mengetahui  pengaruh  formulasi  daun  kakao  dan  kayu  manis  terhadap  penilaian organoleptik, sifat kimia dan aktifitas antioksidan teh daun kakao. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), yang terdiri dari 5 jenis perlakuan formulasi daun kakao dan  kayu manis yaitu daun kakao 100% : kayu manis 0% (KM0), daun kakao 95% : kayu manis 5% (KM1), daun kakao 90% : kayu manis 10% (KM2) daun kakao 85% : kayu manis15% (KM3) dan daun kakao 80% : kayu manis  20% (KM4). Formulasi daun kakao dan kayu manis mempunyai hasil yang berpengaruh nyata (p<0,05) pada uji organoleptik warna dan rasa, namun berpengaruh tidak nyata terhadap parameteraroma. Berdasarkan penilaian organoleptik, teh daun kakao dapat diterima oleh panelis. Kadar air, pH, dan aktivitasantioksidan pada sampel terpilih berturut-turut sebesar 3,96%, 5,12-6,20 dan 39,55%. Berdasarkan hasil tersebut maka teh daun kakao dengan penambahan kayu manis dapat  memenuhi standar teh. Kata kunci: Teh daun kakao,Kayu manis, Organoleptik, Aktivitas antioksidan.   
INCORPORATING MORINGA AND KATUK LEAF EXTRACTS IN THE PRODUCTION OF SAGO BASED LIQUID SUGAR: SENSORY PROPERTIES, GLUCOSE CONTENT AND ANTIOXIDANT ACTIVITY Ansharullah, Ansharullah; Natsir, Muhammad; tamrin, tamrin; Asranudin, Asranudin
Jurnal Sains dan Teknologi Pangan Vol 4, No 5 (2019): JURNAL SAINS DAN TEKNOLOGI PANGAN
Publisher : JURUSAN ILMU DAN TEKNOLOGI PANGAN, UNIVERSITAS HALU OLEO

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (798.859 KB) | DOI: 10.33772/jstp.v4i5.9417

Abstract

ABSTRACT Incorporation of the extracts of Moringa and Katuk leaves in the production of sago based liquid sugar was one way to diversify and improved its added value. The liquid sugar produced had a better anti-oxidant activity, but had a lower sensory properties. A pilot plant scale production of this liquid sugar was attempted in collaboration with a small scale enterprise in Moramo,Southeast Sulawesi. This study was aimed to determine the fortification effect of Moringa and Katuk leaf extract on the properties of sensory, and anti-oxidant activity of the sago liquid sugar. The sugar was produced enzymatically through liquefaction and saccharification processes employing commercial alfa-amylase and amyloglucosidase. The sugar was then fortified with the extract of Moringa and Katuk leaves, with various concentrations, i.e. G0 (100 % liquid sugar as a control); G1 (95% liquid sugar + 5% Katuk extract), G2 (95% liquid sugar + 5% Moringa extract), G3 (90% liquid sugar + 5% Moringa extract + 5% Katuk extract), G4 (85% liquid sugar + 5% Moringa extract + 10% Katuk extract), and G5 (85% liquid sugar + 10% Moringa extract + 5% Katuk extract). The results showed that fortification of Moringa and Katuk leaf extract had a significant effect on the sensory properties, and anti-oxidant activity. The G1 treatment produced better sensory properties. Increasing the content of leaves extracts had also increased the anti-oxidant activity. There was no significant differences in the glucose content of the sugar. Considering various aspects of the results obtained, the G3 formula was then scaled up for pilot plant production by a small scale enterprise. This study had indicated that a nutritional food ingredients and added value may be made through a diversified process of sago flour. Keywords: Moringa, katuk, sago liquid sugar, anti-oxidant, sensory properties INTRODUCTION The use of sago as a food has long been recognized and has even become
Teknologi Tepat Guna Pembuatan Gula Aren Kristal Di Desa Mabodo Kecamatan Kontunaga Kabupaten Muna Sulawesi Tenggara Asranudin, Asranudin; Holilah, Holilah; Sadimanatara, Muh. Syukri; Karimuna, La; Ernawati Harfi, Wa Ode; Josaphat, Josaphat
Jurnal Pengabdian Masyarakat Ilmu Terapan (JPMIT) Vol 1, No 1 (2019)
Publisher : Vokasi Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.253 KB) | DOI: 10.33772/jpmit.v1i1.9578

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Southeast Sulawesi is a region that rich in various agricultural products including cocoa commodities which are widely known to foreign countries. Southeast Sulawesi is divided into two regions, namely the land divided into nine and the islands divided into eight administrative regions. Muna is the Southeast Sulawesi archipelago rich in various agricultural products, especially from sugar palm plants. Specifically, palm sugar is abundant in Mabodo with a plant density of 30m2 so that it has the potential to develop various diversified products. The technology transfer applied to PPTTG activities was a method or process of palm sugar processing into organic crystal sugar. The purpose of the PPTTG was to apply appropriate technology through the stages of Participatory Rural Appraisal (PRA), participatory Technology Development, community development, Persuasive to Educational. To maintain the existence of PPTTG in Mabodo Village, product assistance was carried out such as design and registration of trademark IPRs, packaging. Packaging of product distribution licenses and product socialization to local government stakeholders. In this activity, it can be seen that all the women who are members of “Kelompok Usaha Nira Sejahtera Mabodo” have been skilled in crystallizing of the roomie manually or with crystallizers. This success has opened the gap for the presence of new MSME centers that produce a variety of palm-based diversified products. Eventhough their entrepreneurial spirit was intrigued through their curiosity to process other parts of the sugar palm plant into food products or crafts of high economic value that will sustain the family economy. The sustainability of the technology and products of PPTTG activities requires evaluation and assistance for the next few years that can bring Mabodo Village to become the Aren Tourism Area.
Role of Fe2+-dependent Reaction in Biodecolorization of Methyl Orange by Brown-rot Fungus Fomitopsis pinicola Adi Setyo Purnomo; Asranudin; Nela Rachmawati; Hamdan Dwi Rizqi; Refdinal Nawfa; Surya Rosa Putra
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.146-154

Abstract

The involvement of Fenton reaction on biodegradation of methyl orange (MO) by brown-rot fungus Fomitopsis pinicola was investigated based on Fe2+-dependent reaction. The degradation of MO (final concentration 75 mg/L) was performed in mineral salt media with and without Fe2+ with incubation period at 0, 7, 14, 21, and 28 days. Degradation analysis was performed using UV-Vis Spectrophotometer and LC-TOF/MS. F. pinicola decolorized MO in a medium containing Fe2+ and a medium that lacked the mineral, at percentages of 89.47% and 80.08%, respectively. The optimum decolorization occurred after 28 days of incubation with the fungus on the presence of Fe2+, indicated that the presence of Fe2+ enhanced MO degradation with assumed to correlate with Fenton reaction. Two metabolites were detected through the LC-TOF/MS analysis, namely 4-(2-(4-(dimethyliminio)-2-hydroxycyclohexa-2,5-dien-1-ylidene) hydrazinyl) phenolate (m/z 258, RT: 1.28 min, compound 1) and 4-(2-(4-(dimethyliminio) cyclohexa-2,5-dien-1-ylidene) hydrazinyl) benzenesulfonate (m/z 391, RT: 2.70 min, compound 2). Compound 1 was a transformation product of hydroxylation and methylation, compound 2 was a product of dehydroxylation and desulfonation. This study indicated that the transformation of the metabolite structures was involved hydroxyl radical (OH.) and enzymatic mechanisms, which involved Fe2+-dependent reaction.
Characterization of mannanase-producing bacteria from sago hump Sri Wahyuni; Andi Khaeruni R; Lianto Lianto; Sidarmin Sidarmin; Holilah Holilah; Wahyu Prasetyo Utomo; Asranudin Asranudin
Waste Technology Vol 4, No 1 (2016)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (715.804 KB) | DOI: 10.14710/4.1.1-6

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Sago is a hardwood that can be used as source of fiber hemicellulose mannan. The abundance of mannanase waste is potential for the production of functional foods such as Manno oligosaccharides. Sago hump which is sago processing waste is very potential for growth of microorganisms because it contains lignin, cellulose, starch, minerals and vitamins that can be used as sources of carbon and energy for the growth. In this study, BLS.11-01 and BLS.11-02 isolates have been isolated and characterized from waste of sago hump. Locust bean gum was used as  substrate for measuring the activity of mannanase. Both isolates had optimum temperatures at 70°C and 60°C while they remain stable at temperature range of 30°C - 90°C. Mannanase from BLS.11-01 and BLS.11-02 had optimum pH at 6 and 7, respectively, and were also stable in wide pH range of 2-8. Co2+ was a mannanase activator for both isolates. Cu2+, Zn2+, Ni2+, Ca2+ and Fe2+ were  mannanase inhibitors for two isolates. Tween 20 (0.5% and 1.0%) and NaCl (0.5% and 1.0%) were chemical reagents that could enhance the activity of BLS.11-01 and BLS.11-02 mannanase isolates.  While tween 80 (0.5% and 1.0%), urea (0.5% and 1.0%) and SDS (0.5% and 1.0%) were chemical reagents that were not effective for both isolates mannanase activity
The Biotransformation and Biodecolorization of Methylene Blue by Xenobiotic Bacterium Ralstonia pickettii Adi Setyo Purnomo; Asranudin Asranudin; Didik Prasetyoko; Yulinar Dwi Nur Azizah
Indonesian Journal of Chemistry Vol 21, No 6 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.65806

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The biotransformation and biodecolorization of methylene blue (MB) dye using the bacterium Ralstonia pickettii was investigated. This experiment was conducted in a nutrient broth (NB) medium after adding MB at 100 mg L–1 concentration. Approximately 98.11% of MB was decolorized after 18 h of incubation. In addition, the metabolic products detected by LC-TOF/MS were Azure A (AA), thionine, leuco-MB, and glucose-MB, which indicated the MB degradation through a reductase that attacked the heterocyclic central chromophore group present in the structure. Moreover, azure A and thionine fragments resulted from the N-demethylase enzyme that attacked the auxochrome group. Thus, this research was assumed to be the first scientific report suggesting the potential to use R. pickettii in the biodecolorization and biotransformation of dye waste, particularly MB.
Polyhydroxyalkanoates Production from Ralstonia Pickettii Bacteria: Structural and Mechanical Studies Asranudin Asranudin; Surya Rosa Putra; Adi Setyo Purnomo; Dalia Allouss; Holilah Holilah; Lisman Suryanegara; Alvin Romadhoni Putra Hidayat; Alvin Rahmad Widiyanto; Muchammad Tamyiz
Journal of Fibers and Polymer Composites Vol. 1 No. 2 (2022): Journal of Fibers and Polymer Composites
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (706.887 KB) | DOI: 10.55043/jfpc.v1i2.53

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Bacterial Polyhydroxyalkanoates (PHAs) are a remarkably versatile category of biodegradable polymers with a variety of applications in the packaging, agricultural, biomedical, and pharmaceutical fields. In the present study, bacterial PHAs films are characterized by Fourier transform infrared (FTIR), Scanning electron microscope (SEM), Gas chromatography-mass spectroscopy (GC-MS), Differential scanning calorimetry (DSC), and Universal testing machine (UTM). It was found that almost 20% (w/w) of PHAs was produced from Ralstonia pickettii, and the five major types of the produced polymer were validated via FTIR analysis, i.e., 1046-1185 cm-1 (C-O stretching), 1723 cm-1 (C=O stretching), 2974-2926 cm-1 (CH3 and CH2 stretching) and 3450 cm-1 (OH stretching). The GC-MS chromatogram generated two main peaks, i.e., 2-butenoic acid methyl ester and 4-hexenoic acid methyl ester, at retention times of 4.62 min and 5.79 min, respectively. The main compounds of 2-butenoic acid methyl ester and 4-hexenoic acid methyl ester had percentage areas of 28% and 43%, respectively. Based GCMS analysis shows two monomer PHAs ie. 2-butenoic acid methyl ester and 4-hexenoic acid methyl ester, correspondingly. The blending of PHAs R. pickettii and PEG-400 positively decreases thermal properties and tensile strength and increases elongation at break.
Penggunaan Adsorben dalam Pengolahan Limbah Pewarna dari Kerajinan Tenun Ikat di Desa Parengan, Kecamatan Maduran, Kabupaten Lamongan Sebagai Upaya Mengurangi Pencemaran Pewarna di Sungai Bengawan Solo Adi Setyo Purnomo; Hendro Nurhadi; Refdinal Nawfa; Herdayanto Sulistyo Putro; Hamdan Dwi Rizqi; Muhammad Lukman Hakim; Asranudin; Taufiq Rinda Alkas; Alya Awinatul Rohmah; Eka Pratiwi Yuniarti; Badzlin Nabila
Sewagati Vol 6 No 1 (2022)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.084 KB) | DOI: 10.12962/j26139960.v6i1.188

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Industri tekstil merupakan salah satu industri yang berkembang pesat di Indonesia saat ini. Selain, menghasilkan produk tekstil, industri juga menghasilkan limbah warna. Desa Parengan yang terletak di Kabupaten Lamongan merupakan Desa yang telah dikenal sejak lama sebagai Desa industri penghasil kain tenun ikat. Pembuangan limbah pewarna di Desa tersebut masih dilakukan secara langsung ke badan Sungai Bengawan Solo. Hal tersebut sangat berdampak negatif terhadap biota Sungai, sehingga sangat diperlukan solusi untuk penanganan pengolahan limbah. Tim pengabdian Institut Teknologi Sepuluh Nopember telah membuat teknologi dekolorator berbasis kolom matriks yang mengandung adsorben super adsorpsi, yang merupakan hasil riset laboratorium kimia. Reaktor terhubung dengan kolom matriks yang berisi adsorben arang aktif-silika-zeolit. Sistem ini dapat menghilangkan limbah pewarna menjadi larutan yang aman dibuang di lingkungan. Tim abdimas ITS telah menyerahkan 1 unit reaktor kepada UMKM tenun ikat di Desa Parengan dimana diharapkan akan memberikan dampak signifikan terhadap pengurangan limbah pewarna di Sungai Bengawan Solo. Selain itu, kegiatan ini diharapkan dapat mengubah pola pikir warga desa untuk tetap selalu menjaga ekosistem lingkungan dan menggalakkan terciptanya Desa cerdas lingkungan.