Rinaldi Budi Utomo
Departemen Ilmu Kedokteran Gigi Anak, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta, Indonesia

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Journal : ODONTO Dental Journal

Antibacterial effect of ethanol extract of moringa oleifera seeds against enterococcus faecalis atcc 29212 Pratiwi, Ririn Dwi; Kuswandari, Sri; Utomo, Rinaldi Budi
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.180-187

Abstract

Background: One of the important stages in endodontic treatment is root canal irrigation. The most widely used irrigant is sodium hypochlorite (NaOCl), but it is toxic to periapical tissue. Irrigant solutions from natural ingredients that have antibacterial potential are needed, one of which is Moringa oleifera seeds. Bacteria that are often found persistent after root canal treatment are Enterococcus faecalis. The purpose of this study was to determine the antibacterial effect of the ethanol extract of M. oleifera seeds at concentrations of 37.5%, 50%, 67.5% and 75% against E. faecalis.Method: Experimental laboratory research with post-test only control group design was carried out with 4 times replication. Antibacterial activity was tested against E. faecalis ATCC 29212 using paper disc diffusion method on Mueller Hinton Agar (MHA) media. The diameter of the inhibition zone formed was measured. Data were analysed by one-way ANOVA followed by LSD.Result: The 2.5% NaOCl group produced the largest inhibition zone of 16.38 ± 0.95 mm, followed by the 75% extract group of 13.51 ± 0.49 mm, and the smallest was the 37.5% extract group of 4.42 ± 0.27 mm. The negative control (DMSO) did not produce an inhibition zone. The increase in the concentration of the ethanolic extract of M. oleifera seeds resulted in significantly better inhibiting the growth of E. faecalis (p<0.05).Conclusion: Ethanol extract of M. oleifera seeds at 37.5%, 50%, 62.5% and 75% had antibacterial effects against E. faecalis, with the 75% had the strongest antibacterial effect compared to other extract concentrations. 
COMPARISON OF DENTAL ANXIETY LEVELS MEASURED USING DENTAL ANXIETY SCALE AND GSR-PSYCHOANALYZER IN PATIENTS AGED 6-8 YEARS OLD Imamullah, Adil Yuni; Utomo, Rinaldi Budi; Supartinah, Al
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.1-11

Abstract

Background: Dental anxiety is a feeling of worrying that something unpleasant or unreasonable will happen during dental and oral care so that the child becomes uncooperative. There are two ways to measure dental anxiety in children subjectively and objectively. Subjectively measured using the Dental Anxiety Scale and objectively using the GSR-Psychoanalyzer. The purpose of this study was to determine the comparison of dental anxiety levels measured using the Dental Anxiety Scale and GSR-Psychoanalyzer in patients aged 6-8 years.Method: Anxiety measurements were carried out in the clinic waiting room before treatment for 32 children aged 6-8 years, consisting of 15 boys and 17 girls. There are 12 children aged 6 years, 9 children aged 7 years, and 11 children aged 8 years. The measurement process was done by the first 16 children using the Dental Anxiety Scale than the GSR-Psychoanalyzer and 16 other children were done the other way around.Result: The Chi-Square test showed that there was no significant difference based on gender with a p-value = 0.233 on the Dental Anxiety Scale and a p-value = 0.760 on the GSR-Psychoanalyzer. Chi-Square test results based on age also showed no significant difference with a p-value = 0.540 on the Dental Anxiety Scale and a p-value = 0.475 on the GSR-Psychoanayzer. The level of dental anxiety as measured using the Dental Anxiety Scale and GSR-Psychoanalyzer in children aged 6-8 years after being analyzed using the Wilcoxon Signed Rank Test, showed no significant difference with p=0.322.Conclusion: There is no difference in the level of dental anxiety based on age and gender as measured using the Dental Anxiety Scale and GSR-Psychoanalyzer