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All Journal bionature As-syifaa Jurnal Farmasi Borneo Journal Of Pharmascientech Jurnal Kesehatan Tambusai Journal Microbiology Science Journal of Pharmaceutical and Sciences. Jurnal Novem Medika Farmasi Jurnal Pengabdian Farmasi dan Sains MPSJ

Rachmat Kosman, Rachmat

Universitas Muslim Indonesia

Author-ID : 560858

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

Published : 32 Documents Claim Missing Document

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Articles

Title

Antibacterial Activity Test of Endophytic Fungi Extraction Kasumba Turate (Carthamus tinctorius L.) Leaves From Galesong against Digestive Tract Infection Amin, Muhammad; Kosman, Rachmat; Rusli, Rusli
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1005

Digestive tract infection is still a significant health problem in Indonesia, it caused by pathogenic bacteria. Kasumba turate (Carthamus tinctorius L.) is a medicinal plant that empirically used as measles drug with several compounds such as flavonoids, quinocalcones, polyacetylenes, alkaloids, fatty acids, steroids, proteins and polysaccharides and kasumba turate (Carthamus tinctorius L.) leaves are believed to have antimicrobial activity against several microbes pathogens such as E.coli and S.aureus. This research was conducted to determine endophytic fungi isolates from kasumba turate (Carthamus tinctorius L.) leaves that can provide activity against bacteria that cause digestive tract infections seen from the bioautography profile determination. Three endophytic fungi isolated from kasumba turate (Carthamus tinctorius L.) leaves were purified and observe macroscopically. After purification an antibacterial screening test was conducted by observing the inhibition zone of each isolate; the isolate with largest inhibition zone is IFEDKT-03. Isolates were fermented for 21 days, mycelia and supernatant were separated, and extraction was performed. The endophytic fungi extract of kasumba turate (Chartamus tinctorius L.) leaves has antibacterial activity against Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholera as indicated by Rf values of 0.23, 0.41, 0.49, 0.56, 0.70 and 0.76

LITERATURE STUDY OF ANTIBACTERIAL ACTIVITY OF THE FAMILI RUTACEA Nurfadila, Nurfadila; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 2, No 1 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Indonesia has one of potential plants as an herbal medicine, called Lime (Citrus aurantifolia) and Kaffir Lime (Citrus hystrix DC), both used for different purposes such as cooking, seasoning and traditional medicine. Both plants are derived from the family of Rutaceae which belongs to shrubs that have many branches and twigs. This study aimed to carry out the antibacterial assay of the Rutaceae family. This study used a literature study approach, which references were obtained through 3 Journal sources, namely Google scholar, pubmed, and thscience direct. Furthermore the selected research article which met the criteria was reviewed. Based on the search done, it obtained 7 articles and identified antibacterial activity of lime and Kaffir Lime. The result confirmed that both have antibacterial compounds against bacteria Escherichia coli, and Staphylococcus aureus because they contain secondary metabolite compounds consisting of alkaloids, flavonoids, tannins, and essential oils.

ETHNOPHARMACY STUDY OF PLANTS WITH MEDICINAL PROPERTIES IN NORTH MALUKU PROVINCE Adrias, Cici Rahmayanti; Herwin, Herwin; Jamaluddin, Jamaluddin; Kosman, Rachmat
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

North Maluku Province, especially in Joubela Village, Morotai Island Regency, South Morotai District, is one of the remote areas located in the eastern part of Indonesia. The tribes that still thrive on the island are the Galela and Tobelo, these tribes are some of the largest tribes in North Maluku, recorded to still preserve many plants as traditional medicine. The research aimed to find out the ethnopharmaceutical plants in the village of Joubela. This present study was conducted through direct observation to people who were known to understand the use of traditional medicinal plants such as shamans, community leaders and the public who have used plants for treatment which were selected through purpose sampling method. The results obtained 15 orders, 20 families, 26 genera and 26 species of plants that have empirical potential as medication and the most widely used plant as traditional medicine was garden shrub (Graptophyllum pictum L.), with the efficacy as an ulcer medicine.

IDENTIFICATION OF ACTIVE CHEMICAL COMPONENTS OF ETHANOL EXTRACT OF PAINTED NETTLE LEAVES (COLEUS SCUTELLARIOIDES L. BENTH) AGAINST GASTROINTESTINAL INFECTION BACTERIA USING TLCBIOAUTOGRAPHY AND AGAR DIFFUSION METHOD Junaedi, Fhadillah; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Infectious diseases are one of the public health problems caused by the transmission and multiplication of microorganisms. The research on the antibacterial activity of painted nettle leaves (Coleus scutellarioides L. Benth) extracts was carriedout to examine the activity and chemical components that provide inhibition against gastrointestinal infection bacteria. The extraction of painted nettles leaves performed maceration methd using a solvent of 96%. The extraction results were examined to determine the antibacterial activity at 1% concentration of the test bacteria: Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The results showed that 96% ethanol extract of painted nettle leaves inhibited Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The research conducted using agar diffusion indicated that painted nettleleaf extract showed activity against bacteria and had highly strong potential with diameters of 6-20 mm. The results of Thin Layer Chromatography (TLC) Bioautography test of ethanol extract of painted nettle leaves using chloroform:methanol eluent (3:1) showed inhibition of Escherichia coli, Shigella dysenteriae, and Salmonella thypi at Rf values of 0.90, 0.80, 0.56. Therefore, the identification test confirmed that the chemical componentsproviding antibacterial are from flavonoids, phenols, terpenoids, alkaloids, and steroidsKey words: Identification of chemical components, painted nettle leaves, gastrointestinal bacteria, TLC-Bioautography and Agar diffusion

Antibacterial Activity of Extra Ethanol Kopasanda Leaves (Chromolaena Odorate L.) Against Pathogenic Bacteria of Urinary Tract Infection by TLC-Bioautography and Agar Diffusion Wirawan, Alif Yusra; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.979

Kopasanda (Chromolaena odorata L.) leaves are one of the plants (herbs) containing various types of secondary metabolites, including saponins, tannins, alkaloids, and flavonoids, which function as antibacterial agents used as wound medicine for the community. This study aims to determine the antibacterial activity of the ethanol extract of Kopasanda (Chromolaena odorate L.) leaves using the TLC-bioautography and diffusion methods. The results of the antibacterial activity screening test obtained the active extract at a concentration of 0.1% against Escherichia coli and Staphylococcus aureus. The results of the antibacterial activity test using the TLC-Bioautography method with the eluent N-Hexane:Ethyl Acetate (4:1) showed results from Staphylococcus aureus bacteria with Rf values = 0.90, 0.78, 0.67, 0.52, 0.38, 0.27, 0.14, 0.05, and Escherichia coli bacteria with Rf values = 0.90, 0.52, 0.38, 0.27, 0.14, 0.05. Based on the results of testing the antibacterial activity with the agar diffusion method, the largest diameter of the inhibition zone at a concentration of 10% was obtained for Escherichia coli bacteria with an inhibition zone diameter of 13.59 mm, and Staphylococcus aureus with an inhibition zone diameter of 15.52 mm.

Free Antiradical Activity Test of Endophytic Fungi Isolates of Kasumba Turate (Carthamus tinctorius (L) Leaves from Galesong Takalar with KLT-Autography Method Sari, Dewi Mustika; Kosman, Rachmat; Rusli, Rusli
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1027

The kasumba turate plant (Carthamus tinctorius (L.) has the potential as a free antiradical. Free antiradical compounds in kasumba turate plants come from secondary metabolites produced by endophytic compounds known as endophytic fungi. Kasumba turate contains phenolic compounds and carotenoids that have antioxidant activity. This study aims to obtain isolates of endophytic fungi in kasumba turate (Carthamus tinctorius (L.) plants that can act as free antiradicals by KLT-Autography method. Five isolates IFDK 01, IFDK 02, IFDK 03, IFDK 04, and IFDK 05 were obtained. After testing the antioxidant activity of endophytic fungal isolates, two isolates were obtained that gave the best antioxidant activity, namely isolates IFDK 03 and IFDK 04. The results of KLT-Autography testing by spraying DPPH isolates IFDK 03 and IFDK 04 have the potential as free antiradicals showing yellow spots on a purple background having an Rf1 value, IFDK 03 isolate 0.94 and Rf2 0.55 and Rf1 value, IFDK 04 isolate 0.76 and Rf2 0.43

ISOLATION AND ACTIVITY ANTIBACTERIAL OF ISOLATES ENDOPHYTE FUNGI OF JATROPH MULTIFIDA L. STEM Herwin, Herwin; Kosman, Rachmat; Wahyuni, Sri
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Endophytic fungi that live on plant tissue has the potential to produce secondary metabolites, same as its host. This study aims to isolation endophyte fungi and antibacterial activity of isolate endophyte fungi of Jatropha multifida L. stem to Escheriacia coli and Staphylococcus aureus bacteria. The preliminary research was performed by isolation endophyte fungi of Jatropha multifida L. stem use PDAC medium and the result of isolation endophyte fungi Jatropha multifida L. stem obtained 6 isolates. Isolates endophyte fungi done examination morphology by microskopic method obtained that islolates have different carasteristic. Based on examination of antibacterial activity by agar diffusion method showed that isolates endophyte fungi that potential as antibacterial is isolate IFJT1 code where isolate IFJT1 code active to Escheriacia coli bacteria obtained inhibitory zone diameter of 17,7 mm and to Staphylococcus aureus bacteria.obtained inhibitory zone diameter of 16,7 mm

LITERATURE STUDY OF ANTIBACTERIAL ASSAY OF AVERRHOA BILIMBI L. AGAINST GRAM POSITIVE BACTERIA Sari, Erwika Dwika; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 2, No 1 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Averrhoa bilimbi L. is one of the traditional medicines that people empirically use as medicine to treat various diseases such as cough, diabetes, rheumatism, mumps, canker sores, toothache, bleeding gums, acne, diarrhea to high blood pressure. The research aimed to determine the antibacterial activity of Averrhoa bilimbi L. against gram-positive bacteria. This research applied a literature study method in which the references were obtained from 3 data bases, namely google scholar, pubmed and mdpi, then selected journals that metthe criteria in the literature study. From the journal exploration, 7 journals were obtained. Then the antibacterial activity of Averrhoa bilimbi L. against gram-positive bacteria was observed. The results confirmed that the fruit and leaves of Averrhoa bilimbi L. had an antibacterial activity against Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, and Propionibacterium acnes due to the presence of secondary metabolites, namely saponins, tannins, alkaloids and flavonoid.

Co-Authors Achmad Rosyadi Yusuf Hibai, Achmad Rosyadi Yusuf Adrias, Cici Rahmayanti Amalia.B, Putri Andi Maulana Kamri Aulia Wati, Aulia Azhary, Nursyahidah Basri, Putri Septiani Bayu Putra, Bayu Faradila, Aulia Febrilia Mahmud, Melly Fitriana FITRIANI, SITTI ANNISA Hamdani, Nina Hamsinah Hamsinah, Hamsinah Hativa, Hativa Herwin Herwin, Herwin Ika Indra Wijaya, Ika Indra Ira Damayanti, Ira Irma Santi, Irma Iskandar , A. Nasyrah Jamaluddin Jamaluddin Junaedi, Fhadillah Kurniati Tappang, Kurniati Melinda, Pina Muhammad Amin Muzakkir Baits, Muzakkir Nur Ismayani, Nur Nurfadila Nurfadila, Nurfadila Nurmaya Effendi Nurul Inayah Nurung, Ayyub Harly Pirman AP, Pirman Putra , Bayu R. Rusli Rabbana, Rahmawati Rahmadani, Andi Syafirah Rahman, Safriana Rahmawati, Rahmawati Ramadhani, Fitrah Anggun Riany Novianti Y., Riany Rizqi Nur Azizah Rusli Safriani Rahman, Safriani Sahide, Rusli Sari, Dewi Mustika Sari, Erwika Dwika Siska Nuryanti, Siska Sitti Amirah Sri Wahyuni Sudrianto Sudrianto, Sudrianto Sukmawati Sukmawati Sukmawati Syarif, Sukmawati Wirawan, Alif Yusra Zainal Abidin

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