Article Per Year (5 Year)
p-Index From 2021 - 2026
2.693
P-Index
This Author published in this journals
All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Sciscitatio : Journal for Biological Science Jurnal Atma Inovasia International Journal of Science, Technology and Applications Berita Biologi
To'bungan, Nelsiani
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Physics Social Sciences Other

Published : 27 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 27 Documents
Search

 1   2   3 
Phytochemical Constituent, Antibacterial and Antioxidant Leaves of Dracaena trifasciata (Prain) Mabb. Dewatisari, Whika Febria; To'bungan, Nelsiani
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33528

Abstract

Sansevieria, commonly known as Dracaena trifasciata. Apart from serving as an ornamental and air-purifying plant, it also exhibits medicinal properties, making it a potential candidate for the development of novel natural drugs. The demand for natural antibacterial agents has significantly increased due to the rising incidence of resistance among pathogenic bacteria, such as Staphylococcus aureus. This research conducted to determine the antibacterial and antioxidant activities of D. trifasciata leaf extracts. The D. trifasciata leaf material was sequentially extracted using sonication, beginning with chloroform and then followed by ethanol. The Kirby-Bauer method was employed to conduct the antibacterial test, wherein the inhibition zones generated by the chloroform and ethanolic extracts were measured. The test bacteria used in this study was Staphylococcus aureus. The assessment of the antioxidant activity was conducted using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Phytochemical compound identification in the extracts was conducted using GC-MS analysis. The ethanolic extract showed a higher yield percentage compared to the chloroform extract. Both extracts exhibited moderate antibacterial activity. The chloroform extract exhibited an inhibition zone of 73.33 mm2, while the ethanolic extract showed an inhibition zone of 110 mm2. The chloroform extract had a minimum inhibitory concentration (MIC) of 500 mg/mL and a minimum bactericidal concentration (MBC) of 1000 mg/mL. The ethanolic extract had an MIC of 31.25 mg/mL and an MBC of 62.50 mg/mL. In terms of antioxidant activity, the chloroform extract had an IC50 of 370.8±0.07 µg/mL, while the ethanolic extract had an IC50 of 647.4±0.12 µg/mL. GC-MS analysis revealed 47 compounds in the chloroform extract and 49 compounds in the ethanolic extract. Based on the results of yield, antibacterial and compound identification, the ethanolic extract of D. trifasciata leaves exhibited greater potential compared to the chloroform extract. Its antibacterial activity showed more promise than its antioxidant activity
Standardization of Simplisia of Dutch Teak Leaves (Guazuma ulmifolia) and Root of Mud (Rheum officinale) To'bungan, Nelsiani
International Journal of Science, Technology and Applications Vol 3 No 1 (2025): Volume 3 Number 1 Tahun 2025
Publisher : Alpatih Harapan Semesta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70115/ijsta.v3i1.272

Abstract

Standardization of simplicia is one of the important stages in ensuring the quality of herbal simplia. This study aims to conduct specific and non-specific standardization of simplicia of Dutch teak leaves (Guazuma ulmifolia) and kelembak root (Rheum officinale) based on the parameters of the Indonesian Herbal Pharmacopoeia (FHI) 2017. The parameters tested included moisture content, total ash content, insoluble ash content, drying shrinkage rate, ethanol soluble juice content, and water soluble juice content. The results showed that the simplicia of Dutch teak leaves had a moisture content of 7.90 ± 0.27%, a total ash content of 8.53 ± 0.19%, an acidic insoluble ash content of 1.95 ± 0.52%, drying shrinkage of 4.36 ± 0.28%, the content of ethanol soluble juice 26.20 ± 0.79%, and water-soluble juice 16.29 ± 0.44%. Meanwhile, the root of the pond showed a moisture content of 3.93 ± 0.25%, a total ash content of 5.76 ± 0.90%, an acidic insoluble ash content of 0%, drying shrinkage of 3.23 ± 0.09%, the content of ethanol soluble juice 88.6 ± 1.03%, and water-soluble juice 3.11 ± 0.51%. All non-specific parameters meet the requirements of FHI. The high value of ethanol-soluble juice in the root of the lembak indicates the richness of semipolar compounds, while Dutch teak leaves excel in the content of polar compounds. These results suggest that both simplicia are suitable for use as raw materials for phytopharmaceuticals, either singly or in combination, particularly in the development of herbal products for metabolic disorders
Pelatihan Pengolahan Produk dari Bunga Mawar pada Masyarakat di Kawasan Sapuangin, Merapi, Klaten Widhiastuti, Stefani Santi; Mursyanti, Exsyupransia; Sidharta, Bernardus Boy Rahardjo; Atmodjo, Patricius Kianto; Junedi, Sendy; Arsiningtyas, Ines Septi; To'bungan, Nelsiani
Jurnal Atma Inovasia Vol. 3 No. 6 (2023)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v3i6.8082

Abstract

Roses are one of the commodities in the Sapuangin area, Pemalang, Klaten. The fluctuating selling price and short shelf life make fresh roses only a secondary product. Therefore, a training is needed to process rose flowers into products with high economic value and longer shelf life. This community service aims to enhance the community's knowledge in processing various products from rose flowers. The activities include survey, socialization, training implementation, and evaluation. The training consists of 7 topics from June to July 2023, where participants receive oral explanations and practical demonstrations of the materials provided. The evaluation includes assessing the participants' knowledge through questionnaires given at the beginning and end of the community service, as well as satisfaction evaluation. The entire community service runs smoothly, with participants able to practice making raw materials, solid soap, bath salts, aromatherapy candles, rosewater, and hand sanitizer. Participants also learn about the different types of roses. The average score of the questionnaires from 15 respondents before the training was 21.33 ± 2.61, increasing by 53.49% to 32.74 ± 3.90 from 19 respondents. 68.42% of participants expressed very satisfied, and 31.58% expressed satisfied with participating in the community service activities. In conclusion, the community service has improved the knowledge and skills of the Sapuangin Village community in creating products from rose flowers that have higher economic value and longer shelf life. Keywords — roses, soap, candles, hand sanitizer, rosewater   Abstrak— Mawar merupakan salah satu komoditi di kawasan Sapuangin, Pemalang, Klaten. Harga jual yang tidak menentu dan masa simpan pendek menyebabkan mawar segar hanya menjadi produk sampingan. Oleh karena itu diperlukan kegiatan pelatihan pengolahan bunga mawar menjadi produk dengan nilai ekonomis tinggi dan masa simpan panjang. Pengabdian pada masyarakat ini bertujuan untuk meningkatkan pengetahuan masyarakat dalam mengolah bunga mawar menjadi berbagai macam produk. Kegiatan meliputi tahap survey, sosialisasi, pelaksanaan pelatihan, dan evaluasi. Pelatihan terdiri dari 7 materi mulai Juni-Juli 2023, dimana peserta diberi penjelasan secara lisan dan mempraktekkan secara langsung materi yang diberikan. Evaluasi meliputi evaluasi tingkat pengetahuan melalui kuesioner yang diberikan di awal dan di akhir kegiatan pengabdian, serta evaluasi kepuasan. Seluruh kegiatan pengabdian berjalan lancar, peserta dapat mempraktekkan pembuatan simplisia, sabun padat, garam mandi, lilin aromaterapi, air mawar dan handsanitizer. Peserta juga mengetahui jenis-jenis bunga mawar. Nilai rata-rata kuesioner dari 15 responden sebelum pelatihan adalah 21,33 ± 2,61, meningkat sebesar 53,49% menjadi 32,74 ± 3,90 dari 19 responden. 68,42% peserta menyatakan sangat puas dan 31,58% menyatakan puas mengikuti kegiatan pengabdian. Dapat disimpulkan bahwa kegiatan pengabdian dapat meningkatkan pengetahuan dan kemampuan masyarakat Desa Sapuangin dalam membuat produk dari bunga mawar yang memiliki nilai ekonomis lebih tinggi dengan masa simpan panjang.   
Pelatihan propagasi anggrek skala rumah tangga kepada kelompok wisata di kawasan Sapuangin, Merapi, Klaten Mursyanti, Exsyupransia; Nelsiani To’bungan; Pantalea Edeilweiss Vitara; Bernardus Boy Rahardjo Sidharta; Patricius Kianto Atmodjo; Sendy Junedi; Stefani Santi Widhiastuti
Jurnal Atma Inovasia Vol. 4 No. 3 (2024)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v4i3.8912

Abstract

Sapuangin area is one of supporting areas for Mount Merapi National Park (TNGM). The endemic Merapi orchid is widely cultivated by people in the Sapuangin, using conventional cultivation. Sapuangin’s youth, who are members of a tourism group (POKWIS) have never received training regarding orchid propagation using in vitro culture principles. This is important to support orchid conservation activities carried out by POKWIS in the Sapuangin greenhouse. Based on requests from the community, lecturers and educational laboratory staff concentrating on Technobio-Industrial Studies, Faculty of Biotechnology, Universitas Atma JayaYogyakarta, provided a series of training on in vitro orchid culture, for household scale. This activity is expected to provide knowledge and skills regarding orchid cultivation techniques for the community. The activity was held on 12-20 December 2023. The training activity was divided into several topics, namely introduction to orchid types and their uses, principles of in vitro orchid culture, introduction to tools, methods for sterilizing and sowing orchid seeds, pollination/pollination, overplanting, and acclimatization. The training carried out can provide additional knowledge for the community regarding in vitro orchid cultivation techniques. The implementation of the training was considered very useful and by community needs, the training resource was competent, the implementation time was by the plan, and was supported by complete facilities and infrastructure. The community hopes for ongoing guidance and assistance regarding orchid propagation in the future.
Budidaya dan Pengolahan Pascapanen Berenuk, serta Pembuatan Ekoenzim: Upaya Kegiatan Laudato Si’ di Paroki Wates To'bungan, Nelsiani; Patricius Kianto Atmodjo; Exsyupransia Mursyanti; Boy Rahardjo Sidharta; Junedi, Sendy; Ines Septi Arsiningtyas
Jurnal Atma Inovasia Vol. 5 No. 2 (2025)
Publisher : Lembaga Penelitian dan Pengabdian pada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/jai.v5i2.10291

Abstract

Kegiatan pengabdian masyarakat ini dilakukan di Paroki St. Maria Bunda Penasihat Baik, Wates. Tujuan kegiatan adalah meningkatkan kesadaran dan pemahaman masyarakat tentang prinsip-prinsip Laudato Si' serta pemanfaatan dan budidaya tanaman berenuk (Crescentia cujete) serta pembuatan ekoenzim sebagai salah satu bentuk pengolahan sampah organik. Metode yang digunakan meliputi survei lokasi, sosialisasi, pelatihan, dan evaluasi. Pelatihan mencakup pembuatan produk berbasis berenuk seperti sari buah, masker-jelly, teh daun berenuk, serta pemanfaatan sampah organik menjadi ekoenzim. Hasil kegiatan menunjukkan peningkatan pengetahuan warga gereja tentang pemanfaatan tanaman berenuk sebagai sumber senyawa fitokimia yang bermanfaat serta metode pengolahan sampah menjadi produk bernilai guna. Evaluasi menunjukkan tingkat kepuasan masyarakat yang tinggi terhadap pelaksanaan kegiatan ini. Program ini tidak hanya meningkatkan kesadaran lingkungan tetapi juga memberikan keterampilan baru yang aplikatif kepada masyarakat setempat.
Analisis Bioinformatika NADH2 sebagai Penanda Seleksi Adaptif Pesut Perairan Tawar dan Pesisir Budi, Trifan; Krisnamurti, Gabriella Chandrakirana; To'bungan, Nelsiani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 1 (2023): February 2023
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i1.5631

Abstract

Pesut merupakan mamalia perairan bersifat fakultatif yang dapat ditemui di wilayah Asia Tenggara. Populasi pesut, terutama pada perairan tawar menghadapi berbagai tekanan untuk dapat bertahan hidup sehingga digolongkan sebagai Critically Endangered pada daftar merah IUCN. Pemahaman mengenai sejarah evolusi dan aspek apa yang memfasilitasi adaptasi pesut pada perairan tawar belum banyak diketahui. Adaptasi penuh terhadap perairan tawar pada beberapa spesies lumba-lumba menunjukan adanya seleksi positif pada gen NADH subunit 2 (NADH2) di DNA mitokondria yang diperkirakan berkaitan dengan mekanisme translokasi jalur proton untuk efisiensi energi yang diperlukan dalam kolonisasi perairan tawar. Oleh sebab itu, penelitian ini dilakukan untuk mengetahui apakah gen NADH subunit 2 (NADH2) pada DNA mitokondria memfasilitasi kolonisasi pesut pada perairan tawar seperti dijumpai pada Cetacea perairan tawar lain. Melalui pendekatan bioinformatika sederhana, hasil dari penelitian ini menunjukan tidak adanya subtitusi basa yang merubah susunan asam amino Threonin menjadi Alanin pada urutan asam amino ke 297 gen NADH2 pada populasi pesut yang mendiami perairan tawar dan pesisir. Hal ini menunjukan tidak adanya seleksi adaptif pada gen NADH2 yang diperkirakan terjadi akibat kolonisasi perairan tawar yang relatif baru,  sehingga perubahan asam amino belum teramati pada populasi pesut.
FREE RADICAL INHIBITORY ACTIVITY AND PHYTOCHEMICAL PROPERTIES ETHANOLIC EXTRACT OF Guazuma ulmifolia LEAVES AND Rheum officinale ROOTS To'bungan, Nelsiani; Patricius Kianto Atmodjo; Yulistiyanto, Anggoro Chandra
Berita Biologi Vol 24 No 1 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.8958

Abstract

Despite the unique characteristics, the investigation of the antioxidant activity of ethanol extract of Guazuma ulmifolia leaves and Rheum officinale roots extracted by sonication method and associated with phytochemical content, especially rutin compound content has never been conducted. Therefore, this study aimed to evaluate the antioxidant activity and content of phytochemical compounds in ethanol extracts of G. ulmifolia leaves and R. officinale roots. Extraction was conducted through the sonication method using absolute ethanol solvents, followed by qualitative phytochemical testing and rutin quantitative analysis with UV-Vis spectrophotometry. Free radical inhibitor activity (antioxidant activity) was measured through the DPPH method. The results showed that extract of G. ulmifolia leaves contained alkaloids, flavonoids, tannins, and steroids, while R. officinale roots had only flavonoids and tannins. The rutin concentration was higher in G. ulmifolia compared to R. officinale root. However, the antioxidant activity of R. officinale roots (IC50 = 21.14±2.17 μg/mL) was higher than extract of G. ulmifolia leaves (IC50 = 46.05±1.93 μg/mL). The synergistic or antagonistic interaction of phytochemical compounds in the crude extract caused this variation. Consequently, further studies were recommended to identify phytochemical compounds' synergy mechanisms or antagonisms contributing to biological activity.
Co-Authors Arsiningtyas, Ines Septi Atmodjo, Patricius Kianto Bernardus Boy Rahardjo Sidharta Boy Rahardjo Sidharta Boy Rahardjo Sidharta Boy Rahardjo Sidharta Budi, Trifan Cahyadi, Martin Aristo Exsyupransia Mursyanti Exsyupransia Mursyanti Exsyupransia Mursyanti, Exsyupransia Felicia Zahida Felicia Zahida Hida, Fitriana Nur Laissya Ines Septi Arsiningtyas Ines Septi Arsiningtyas Jajah Fachiroh, Jajah Krisnamurti, Gabriella Chandrakirana Mahardhika, I Wayan Swarautama Martin Aristo Cahyadi Nastiti Wijayanti P. Kianto Atmodjo Pantalea Edeilweiss Vitara Patricius Kianto Atmodjo Sendy Junedi Sidharta, Bernardus Boy Rahardjo Whika Febria Dewatisari, Whika Febria Wibowo Nugroho Jati Wibowo Nugroho Jati Widhiastuti, Stefani Santi Yulistiyanto, Anggoro Chandra ‘Aliyah, Siti Hamidatul