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Decky D. W. Kamagi
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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Analisis Kandungan Nutrisi pada Daging Tikus Hutan Ekor Putih (Maxomys hellwandii) Kamagi, Decky W.; Rompas, Christny Ferdina; Bawole, Alpon Trisno
JSME (Jurnal Sains, Matematika & Edukasi) Vol 8, No 2 (2020): MARET - APRIL
Publisher : Universitas Negeri Manado

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Abstract

White-tailed forest rats (Maxomys hellwandii) are other meat-producing animals that are consumed by the Minahasa community as exotic food. This study aims to determine the nutritional content of white-tailed forest rat meat. Nutrient content analysis was performed at the UNIMA chemistry laboratory and the BARISTAND laboratory in Manado. The procedure for this study is the analysis of nutrient content using the procedure of the Association of Official Analytical Chemists (AOAC 2005). The results of research on fresh forest rat meat showed the content of water, ash, fat, crude fiber, protein and carbohydrates 71.17%, 0.97%, 3.17%, 0.03%, 19, 16%, 5.53% and roasted forest rat meat, i.e. 69.14% water, 1.29% fat and 4.5% protein   
ISOLASI DAN AMPLIFIKASI mtDNA AYAM HUTAN MERAH DAN AYAM KAMPUNG (Gallus gallus) SULAWESI UTARA Kamagi, Decky D. W.
JSME (Jurnal Sains, Matematika & Edukasi) Vol 5, No 2 (2017): Jurusan Biologi
Publisher : Universitas Negeri Manado

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Abstract

Penelitian ini bertujuan memperoleh metode yang tepat untuk isolasi dan amplifikasi sekuens gen cyt b dan COI mtDNA ayam hutan merah dan ayam kampung (Gallus gallus) Sulawesi Utara. Sampel diperoleh dari jaringan otot dari spesimen yang dikumpulkan dari beberapa daerah dan kawasan Sulawesi Utara. Pengumpulan dan preparasi sampel dilakukan dengan perlakuan alkohol 95% dan disimpan dalam suhu dibawah 50C. Isolasi DNA total menggunakan innu PREP DNA micro kit dengan memodifikasi protokol. Amplifikasi gen cyt b menggunakan primer umum L14841 dan H15149, sedangkan amplifikasi gen COI menggunakan primer BirdR1 dan BirdF1. Isolasi DNA dan amplifikasi DNA target dikerjakan di Laboratorium Molekuler Universitas Negeri Manado. Hasil yang diperoleh Metode preparasi sampel dan isolasi DNA total ternyata menghasilkan isolat yang berkualitas yang untuk proses amplifikasi DNA target. Preparasi komponen dan kondisi PCR yang dilakukan ternyata berhasil mengamplifikasi kedua gen target pada ayam hutan dan ayam kampung yaitu gen cyt b dengan panjang berkisar 400 nukleotida dan COI berkisar 600 nukleotida. Kata kunci: Isolasi dan amplifikasi, mtDNA, Ayam, Sulawesi Utara
IDENTIFIKASI MOLEKULER BAKTERI PUPUK ORGANIK SISTIM BIOPORI MENGGUNAKAN GEN 16S rRNA Feren Pangerapan; Suddin Simanjuntak; Decky Kamagi
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

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Abstract

Bakteri dari pupuk organik sistim biopori masih perlu ditentukan kedudukan dalam klarifikasi bakteri. Untuk itu perlu dilakukan identfikasi pada tingkat spesies menggunaan analisis sekuen gen 16S rRNA. DNA bakteri dari pupuk organik sistim biopori di ekstrak untuk di identifikasi secara molekuler menggunakan gen 16S rRNA. Isolat yang diperoleh berasal dari sampel pupuk organik sistim biopori. Bakteri di isolasi pada media NA dengan metode tabur dan di inkubasi pada suhu kamar selama 2x24 jam. Isolate yang tumbuh pada media NA diekstrasi menggunakan Presto Mini gDNA Bacteria KIT. Amplifikasi gen 16S rRNA dengan metode PCR, visualisasi amplikon gen 16S rRNA dengan metode elektroforesis, Amplifikasi DNA target dilakukan dengan menggunakan primer 16sA dan 16sB2. Amplicon-amplikon dielektroforesis menggunakan gel agarose 1,6 dengan DNA Ladder 500-1500bp. Sekuensing menggunakan jasa sekuensing First Base Singapura. Hasil analisis penyelarasan menggunakan program BLAST (www.ncbi.nih.gov.com) 16S rRNA urutan isolat bakteri pupuk organik bp4 sistim biopori menunjukkan kemiripan 98% dengan Bacillus cereus strain MH19 (nomor tambahan CP039269.1). Hasil rekonstruksi filogeni dengan Neighbour Joining, urutan gen 16S Rrna isolate bp4 menunjukkan hubungan terdekat dengan Bacillus cereus strain MH19 (nomor tambahan CP039269.1).
WHITE TAILED JUNGLE RAT MORPHOLOGICAL DIVERSITY STUDY (Maxomys sp.) OF NORTH SULAWESI ACCORDING FROM THE TAIL MORPHOLOGICAL CHARACTERISTICS frisky fransiskus patuwo; Decky D. W. Kamagi; Mariana Rengkuan
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

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Abstract

Tail characteristics of the White-Tailed Jungle Rat (Maxomys sp.) of North Sulawesi have been examined by us to reveal the population diversity. Usually, examining population diversity or species uses numerous morphological features as markers for variation. However, in this study, we are only using one morphological feature, which is the characteristic of the tail from the samples. Several experts have used tail characteristics as the sole identifier for species identifications. On another account, diversity studies using various white-tailed jungle rats are also being performed, and the result of the said study can be compared with the result of the study in which only a single feature is the tail characteristics of the white-tailed jungle rat. The characteristics of white-tailed jungle rats that were examined include total length, the total length of the white section and the present/lack of fine hairs on the tip. The number of samples is 35. The samples are obtained from the jungles of North Sulawesi, which are The District of Minahasa, North Minahasa, South Minahasa, and Southeast Minahasa. The results of morphological observation of the white-tailed jungle rat were analyzed using mutual cluster analysis, statistics program, SPSS IBM, 26. The analysis results from a dendrogram showed two main clusters: the first with the numbers 9, 19 and 12 and the second with the remaining sample numbers. On the second cluster, there are two more formed sub-cluster. The results of this study are nearly identical to the study results that use various morphological diversities. This study recommends that the tail characteristics of a white-tailed rat can represent the diversity of white-tailed rats.
Base Substitution Patterns in Partial of the Cytochrome C Oxidase Unit I (COI) mtDNA Genes in the Red Junglefowl (Gallus sp.) of North Sulawesi and some Gallus sp. accessions. Decky Kamagi
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

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Abstract

Partial of the cytochrome C oxidase unit I (COI) mtDNA genes of the red jungle fowl (Gallus sp.) were isolated and sequenced to examine the base substitution pattern compared to several accessions taken from GenBank. Samples were collected from several areas in North Sulawesi in the form of muscle tissue and prepared with 95% alcohol, and stored at temperatures below 500 C. Total DNA was isolated using the innuPREP DNA micro kit with a modified protocol. The target gene amplification used the primers BirdR1 and BirdF1, and the amplicons were sequenced at the Singapore FirstBase laboratory. Target gene sequences have been blasted through the nucleotide blast program provided by the National Center for Biotechnology Information (NCBI)—sequence analysis using MEGA5 and DnaSPv5 software. Target gene sequences were aligned using the Clustal-W program on MEGA5. The amplified target gene sequence length was 490 bp. The blast results showed that the target gene sequence was 98% identical to the COI gene sequence from several Gallus-gallus accessions from GenBank. The results of multiple alignments between the sequences of some of the genes examined showed the presence of polymorphic sites (S). The number of polymorphic sites (S) is 11, while the conserved sequence (C) is 386/397 = 0.977. The number of haplotypes (h) is 5, and the diversity of haplotypes (Hd) is 0.703. The overall genetic distance average was 0.008. The polymorphic sites and base substitution events occurred at sites number 145 (A↔G), 241 (G↔C), 268 (C↔A), 295 (C↔A), 348 (T↔C), 381(A) ↔T), 384 (T↔C), 390 (T↔A), 393 (C↔T), 394 (T↔C) and 395 (C↔T). The substitution pattern at these polymorphic sites consists of substitution transitions and transversions. The ratio of transition substitution and transversion events (Ts/Tv) = 1.4056. The substitution events at these sites are located at the bases in the first and third positions of the codon. Substitution events at these polymorphic sites partially cause the exchange of amino acids. The exchange of amino acids occurs at codons 49, 81, 90, 99 and 130.
Uji Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) Sebagai Insektisida Alami Lalat Rumah (Musca domestica) Melany Piri; Herry M Sumampouw; Emma Mauren Moko; Decky W Kamagi; Helen Lawalata
JURNAL BIOS LOGOS Vol. 12 No. 2 (2022): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v12i2.41034

Abstract

The house fly (Musca domestica) is a vector of mechanical transmission of infectious diseases such as diarrhea, dysentery, cholera, typhoid. One way to control house flies is to use plant-based insecticides. Papaya leaves (Carica papaya L.) can be used as insecticides because of the enzymes saponins, flavonoids, and papain which are toxic to insects. This research aims to determine the effectiveness of papaya leaf extract as a natural insecticide for house flies. The concentration of papaya leaf extract used was 0%, 30%, 40%, 50%, and 60% with 3 replications. Each treatment unit contained 10 house flies. Extract application by spraying directly on house flies. The number of house fly deaths was calculated at 1, 2, 3, 4, 5, 6 and 24 hours. Data were analyzed using One Way Anova test. The result of this research was all the sig values are 0.00 < 0.05, which means that there was a significant difference in the number of house flies deaths at each concentration. In conclusion, the most effective concentration of papaya leaf extract to kill 100% of house flies was at a concentration of 60% after 6 hours of treatment.Keywords: house fly; natural insecticide; papaya leaf ABSTRAKSalah satu vektor penularan penyakit menular mekanis seperti diare, disentri, kolera, tipes adalah lalat rumah (Musca domestica). Salah satu cara pengendalian lalat rumah adalah menggunakan insektisida nabati. Daun pepaya (Carica papaya L.) mengandung enzim saponin, flavonoid, dan papain yang bersifat racun bagi serangga sehingga dapat digunakan sebagai insektisida alami atau insektisida nabati. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas ekstrak daun pepaya sebagai insektisida nabati lalat rumah menggunakan 5 perlakuan konsentrasi ekstrak kasar daun pepaya yakni 0%, 30%, 40%, 50%, dan 60% serta 3 ulangan, setiap unit perlakuan menggunakan 10 ekor lalat rumah, aplikasi ekstrak kasar daun pepaya dengan cara disemprotkan pada lalat rumah, jumlah kematian lalat rumah dihitung pada jam ke-1,2,3,4,5,6 dan 24 jam setelah aplikasi. Data dianalisis menggunakan uji One Way Anova. Hasil penelitian diperoleh semua nilai sig 0,00 < 0,05 yang berarti terdapat perbedaan bermakna jumlah kematian lalat pada setiap pemberian konsentrasi ekstrak daun pepaya. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak daun pepaya yang paling efektif untuk membunuh 100% lalat rumah adalah konsentrasi 60% setelah 6 jam perlakuan.Kata kunci: daun pepaya; insektisida alami; lalat rumah
PEMBELAJARAN DIFERENSIASI MENGGUNAKAN ASSIGN OPEN ENDED DALAM PEMBELAJARAN BIOLOGI MATERI PEMANASAN GLOBAL DI SMA KRISTEN SONDER DENGAN PENELITIAN TINDAKAN KELAS (PTK) Indah Tumelap; Meity Tanor; Decky Kamagi
SOSCIED Vol 6 No 2 (2023): SOSCIED - November 2023
Publisher : LPPM Politeknik Saint Paul Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32531/jsoscied.v6i2.732

Abstract

Implementasi merdeka belajar menuntut harus ada strategi pembelajaran diferensiasi. Pembelajaran diferensiasi adalah salah satu alternatif pembelajaran yang dapat digunakan untuk memenuhi kebutuhan murid dikelas. Tujuan dalam penelitian ini adalah untuk mengetahui hasil belajar dan aktivitas belajar siswa dalam pembelajaran differensiasi menggunakan pendekatan assign open–ended materi pemanasan global pada siswa kelas X SMA Kristen Sonder. Jenis penelitian yang digunakan adalah Penelitian Tindakan Kelas. Subjek penelitian yaitu siswa kelas X yang berjumlah 16 orang siswa. Teknik pengumpulan data dan instrumen penelitian yang digunakan adalah tes dan observasi. Secara klasikal hasil belajar siswa melalui pembelajaran differensiasi menggunakan pendekatan assign open–ended materi pemanasan global pada siklus I yaitu 56.3 % meningkat pada siklus II sebesar 87.5% dan nilai persentasi aktivitas belajar siswa pada siklus I sebesar 50%, meningkat pada siklus 2 yakni sebesar 85.7%. Dengan demikian dapat disimpulkan bahwa pembelajaran diferensiasi menggunakan pendekatan assign open–ended pada materi pemanasan global dapat meningkatkan hasil belajar dan aktivitas belajar siswa kelas X di SMA Kristen Sonder.
IDENTIFIKASI MOLEKULER BAKTERI PUPUK ORGANIK SISTIM BIOPORI MENGGUNAKAN GEN 16S rRNA Feren Pangerapan; Suddin Simanjuntak; Decky Kamagi
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i1.4414

Abstract

Bakteri dari pupuk organik sistim biopori masih perlu ditentukan kedudukan dalam klarifikasi bakteri. Untuk itu perlu dilakukan identfikasi pada tingkat spesies menggunaan analisis sekuen gen 16S rRNA. DNA bakteri dari pupuk organik sistim biopori di ekstrak untuk di identifikasi secara molekuler menggunakan gen 16S rRNA. Isolat yang diperoleh berasal dari sampel pupuk organik sistim biopori. Bakteri di isolasi pada media NA dengan metode tabur dan di inkubasi pada suhu kamar selama 2x24 jam. Isolate yang tumbuh pada media NA diekstrasi menggunakan Presto Mini gDNA Bacteria KIT. Amplifikasi gen 16S rRNA dengan metode PCR, visualisasi amplikon gen 16S rRNA dengan metode elektroforesis, Amplifikasi DNA target dilakukan dengan menggunakan primer 16sA dan 16sB2. Amplicon-amplikon dielektroforesis menggunakan gel agarose 1,6 dengan DNA Ladder 500-1500bp. Sekuensing menggunakan jasa sekuensing First Base Singapura. Hasil analisis penyelarasan menggunakan program BLAST (www.ncbi.nih.gov.com) 16S rRNA urutan isolat bakteri pupuk organik bp4 sistim biopori menunjukkan kemiripan 98% dengan Bacillus cereus strain MH19 (nomor tambahan CP039269.1). Hasil rekonstruksi filogeni dengan Neighbour Joining, urutan gen 16S Rrna isolate bp4 menunjukkan hubungan terdekat dengan Bacillus cereus strain MH19 (nomor tambahan CP039269.1).
WHITE TAILED JUNGLE RAT MORPHOLOGICAL DIVERSITY STUDY (Maxomys sp.) OF NORTH SULAWESI ACCORDING FROM THE TAIL MORPHOLOGICAL CHARACTERISTICS frisky fransiskus patuwo; Decky D. W. Kamagi; Mariana Rengkuan
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i3.5499

Abstract

Tail characteristics of the White-Tailed Jungle Rat (Maxomys sp.) of North Sulawesi have been examined by us to reveal the population diversity. Usually, examining population diversity or species uses numerous morphological features as markers for variation. However, in this study, we are only using one morphological feature, which is the characteristic of the tail from the samples. Several experts have used tail characteristics as the sole identifier for species identifications. On another account, diversity studies using various white-tailed jungle rats are also being performed, and the result of the said study can be compared with the result of the study in which only a single feature is the tail characteristics of the white-tailed jungle rat. The characteristics of white-tailed jungle rats that were examined include total length, the total length of the white section and the present/lack of fine hairs on the tip. The number of samples is 35. The samples are obtained from the jungles of North Sulawesi, which are The District of Minahasa, North Minahasa, South Minahasa, and Southeast Minahasa. The results of morphological observation of the white-tailed jungle rat were analyzed using mutual cluster analysis, statistics program, SPSS IBM, 26. The analysis results from a dendrogram showed two main clusters: the first with the numbers 9, 19 and 12 and the second with the remaining sample numbers. On the second cluster, there are two more formed sub-cluster. The results of this study are nearly identical to the study results that use various morphological diversities. This study recommends that the tail characteristics of a white-tailed rat can represent the diversity of white-tailed rats.
BASE SUBSTITUTION PATTERNS IN PARTIAL OF THE CYTOCHROME C OXIDASE UNIT I (COI) mtDNA GENES IN THE RED JUNGLEFOWL (Gallus sp.) of NORTH SULAWESI AND SOME Gallus sp. ACCESSIONS Decky Kamagi
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i1.6610

Abstract

Partial of the cytochrome C oxidase unit I (COI) mtDNA genes of the red jungle fowl (Gallus sp.) were isolated and sequenced to examine the base substitution pattern compared to several accessions taken from GenBank. Samples were collected from several areas in North Sulawesi in the form of muscle tissue and prepared with 95% alcohol, and stored at temperatures below 500 C. Total DNA was isolated using the innuPREP DNA micro kit with a modified protocol. The target gene amplification used the primers BirdR1 and BirdF1, and the amplicons were sequenced at the Singapore FirstBase laboratory. Target gene sequences have been blasted through the nucleotide blast program provided by the National Center for Biotechnology Information (NCBI)—sequence analysis using MEGA5 and DnaSPv5 software. Target gene sequences were aligned using the Clustal-W program on MEGA5. The amplified target gene sequence length was 490 bp. The blast results showed that the target gene sequence was 98% identical to the COI gene sequence from several Gallus-gallus accessions from GenBank. The results of multiple alignments between the sequences of some of the genes examined showed the presence of polymorphic sites (S). The number of polymorphic sites (S) is 11, while the conserved sequence (C) is 386/397 = 0.977. The number of haplotypes (h) is 5, and the diversity of haplotypes (Hd) is 0.703. The overall genetic distance average was 0.008. The polymorphic sites and base substitution events occurred at sites number 145 (A↔G), 241 (G↔C), 268 (C↔A), 295 (C↔A), 348 (T↔C), 381(A) ↔T), 384 (T↔C), 390 (T↔A), 393 (C↔T), 394 (T↔C) and 395 (C↔T). The substitution pattern at these polymorphic sites consists of substitution transitions and transversions. The ratio of transition substitution and transversion events (Ts/Tv) = 1.4056. The substitution events at these sites are located at the bases in the first and third positions of the codon. Substitution events at these polymorphic sites partially cause the exchange of amino acids. The exchange of amino acids occurs at codons 49, 81, 90, 99 and 130.
Co-Authors Agustina, Tika Puri Bawole, Alpon Trisno Christny Ferdina Evie Rompas Emma M Moko Feren Pangerapan Fernando Andre Watung frisky fransiskus patuwo Hasmiati Hasmiati Helen Joan Lawalata Herry Maurits Sumampouw Indah Tumelap IRIANI SETYAWATI Jein Reflin Herung Mariana Rengkuan Marthy L.S. Taulu Meity Nelltje Tanor Melany Piri Mokosuli Yermia Samuel Montela Waney Motulo, Serlin J Musma Rukmana Suddin Simanjuntak Supit, Annely